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Yuan Mei,Qiyu Song 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.9
Based on Winkler elastic foundation beam theory, Peck modified formula and Laplace transform are used to solve the approximate differential equation of the deflection curve. The settlement calculation method for important homogeneous structures where the tunnel passes underneath is studied. The analytical solution for the settlement of the homogeneous structure is derived and verified. The field monitoring and numerical analysis results are compared. Laplace transform method requires fewer parameters to solve the approximate differential equation of the deflection curve, and its calculation efficiency is high, and the result is the same as that of the classical literature. The method can be applied to practical engineering, and satisfactory results can be obtained. The analytical process in this paper provides a reference for solving similar problems, and the solution can be used for ground settlement and providing an early warning for settlement when the underground engineering passes through the important homogeneous structure from below.
Yuanmei Chen,Yutang Xiao,Guanping Wang,Wei Shi,Linquan Sun,Yanfang Chen,Aichun Miao 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.4
In this pilot-scale test, the ozonation-biological treatment-catalytic ozonation system was performed to treat complex organics and highly-concentrated total nitrogen (TN) in biological pretreated incineration leachate. The test results showed that the ratio of five-day biochemical oxygen demand (BOD5) / chemical oxygen demand (COD) increased from 0.059 to 0.237, which indicated that the concentration of biodegradable COD (CODbio) increased by ozonation pre-treatment process. In addition, the TN removal mainly occurred in anaerobic zone due to direct denitrification by the activated bacteria, which were domesticated through different influent ratio. Moreover, it was necessary to add catalytic ozonation process to reach higher direct effluent discharge criteria. After 60 days repeated debugging, the removal rate of COD and TN reached 88.5% and 98.2%, respectively. Finally, the total cost of this system was ¥ 6.65 /㎥ ($ 0.95 /㎥), which was acceptable for the treatment of biological pretreated leachate. This pilot-scale test could provide some guiding information for the treatment of leachate containing highly-concentrated TN with low CODbio/N by the composite system.
Jiuxiu Ji,Lisheng Zhou,Yuanmei Guo,Lusheng Huang,Junwu Ma 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8
Objective: Growth-related traits are important economic traits in the swine industry. However, the genetic mechanism of growth-related traits is little known. The aim of this study was to screen the candidate genes and molecular markers associated with body dimension and body weight traits in pigs. Methods: A genome-wide association study (GWAS) on body dimension and body weight traits was performed in a White Duroc×Erhualian F2 intercross by the illumina PorcineSNP60K Beadchip. A mixed linear model was used to assess the association between single nucleotide polymorphisms (SNPs) and the phenotypes. Results: In total, 611 and 79 SNPs were identified significantly associated with body dimension traits and body weight respectively. All SNPs but 62 were located into 23 genomic regions (quantitative trait loci, QTLs) on 14 autosomal and X chromosomes in Sus scrofa Build 10.2 assembly. Out of the 23 QTLs with the suggestive significance level (5×10–4), three QTLs exceeded the genome-wide significance threshold (1.15×10–6). Except the one on Sus scrofa chromosome (SSC) 7 which was reported previously all the QTLs are novel. In addition, we identified 5 promising candidate genes, including cell division cycle 7 for abdominal circumference, pleiomorphic adenoma gene 1 and neuropeptides B/W receptor 1 for both body weight and cannon bone circumference on SSC4, phosphoenolpyruvate carboxykinase 1, and bone morphogenetic protein 7 for hip circumference on SSC17. Conclusion: The results have not only demonstrated a number of potential genes/loci associated with the growth-related traits in pigs, but also laid a foundation for studying the genes’ role and further identifying causative variants underlying these loci.
Tang, Jianhong,Zhang, Zhiyan,Yang, Bin,Guo, Yuanmei,Ai, Huashui,Long, Yi,Su, Ying,Cui, Leilei,Zhou, Liyu,Wang, Xiaopeng,Zhang, Hui,Wang, Chengbin,Ren, Jun,Huang, Lusheng,Ding, Nengshui Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.1
Objective: Three genome-wide association studies (GWAS) and a meta-analysis of GWAS were conducted to explore the genetic mechanisms underlying variation in pig teat number. Methods: We performed three GWAS and a meta-analysis for teat number on three pig populations, including a White Duroc${\times}$Erhualian $F_2$ resource population (n = 1,743), a Chinese Erhualian pig population (n = 320) and a Chinese Sutai pig population (n = 383). Results: We detected 24 single nucleotide polymorphisms (SNPs) that surpassed the genome-wide significant level on Sus Scrofa chromosomes (SSC) 1, 7, and 12 in the $F_2$ resource population, corresponding to four loci for pig teat number. We highlighted vertnin (VRTN) and lysine demethylase 6B (KDM6B) as two interesting candidate genes at the loci on SSC7 and SSC12. No significant associated SNPs were identified in the meta-analysis of GWAS. Conclusion: The results verified the complex genetic architecture of pig teat number. The causative variants for teat number may be different in the three populations
Chengbin Wang,Hao Chen,Xiaopeng Wang,Zhongping Wu,Weiwei Liu,Yuanmei Guo,Jun Ren,Nengshui Ding 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12
Objective: Copy number variations (CNVs) are a major source of genetic diversity complementary to single nucleotide polymorphism (SNP) in animals. The aim of the study was to perform a comprehensive genomic analysis of CNVs based on high density whole-genome SNP markers in Chinese Dongxiang spotted pigs. Methods: We used customized Affymetrix Axiom Pig1.4M array plates containing 1.4 million SNPs and the PennCNV algorithm to identify porcine CNVs on autosomes in Chinese Dongxiang spotted pigs. Then, the next generation sequence data was used to confirm the detected CNVs. Next, functional analysis was performed for gene contents in copy number variation regions (CNVRs). In addition, we compared the identified CNVRs with those reported ones and quantitative trait loci (QTL) in the pig QTL database. Results: We identified 871 putative CNVs belonging to 2,221 CNVRs on 17 autosomes. We further discarded CNVRs that were detected only in one individual, leaving us 166 CNVRs in total. The 166 CNVRs ranged from 2.89 kb to 617.53 kb with a mean value of 93.65 kb and a genome coverage of 15.55 Mb, corresponding to 0.58% of the pig genome. A total of 119 (71.69%) of the identified CNVRs were confirmed by next generation sequence data. Moreover, functional annotation showed that these CNVRs are involved in a variety of molecular functions. More than half (56.63%) of the CNVRs (n = 94) have been reported in previous studies, while 72 CNVRs are reported for the first time. In addition, 162 (97.59%) CNVRs were found to overlap with 2,765 previously reported QTLs affecting 378 phenotypic traits. Conclusion: The findings improve the catalog of pig CNVs and provide insights and novel molecular markers for further genetic analyses of Chinese indigenous pigs.