An Equivalent Modeling Method for Honeycomb Sandwich Structure Based on Orthogonal Anisotropic Solid Element

Yuan Jian,Zhang Lei,Huo Zhanwei 한국항공우주학회 2020 International Journal of Aeronautical and Space Sc Vol.21 No.4

An equivalent modeling method for honeycomb sandwich structure is presented in this paper. Honeycomb core is regarded as an interlayer and orthogonal anisotropic solid elements are used to model it, while the panels of honeycomb sandwich structure are represented by shell elements. This method not only controls model size and ensures computational efficiency, but also solves the problem that two-dimensional model cannot represent the internal stress distribution and local deformation. Based on the orthogonal anisotropy of honeycomb and the actual cellular size, 9 independent elastic parameters of the interlayer are given, so that the physical properties of the interlayer are described completely. In the example, the displacement errors under typical static loadcases are less than 3.12% and the frequency errors of the first six orders are less than 4.07%, compared with the precise model. A modal tapping test was carried out on a payload mounting panel with honeycomb sandwich structure. By comparing the test data with the analysis data of the equivalent model, it was shown that the frequency errors of the first six orders were all within 5%, and the analysis modes were consistent with the experimental fitting modes, which further verified the validity of the equivalent method.

Reactive Oxygen Species Scavenging Hydrogel Regulates Stem Cell Behavior and Promotes Bone Healing in Osteoporosis

Ye Yuanjian,Zhong Haobo,Huang Shoubin,Lai Weiqiang,Huang Yizhi,Sun Chunhan,Zhang Yanling,Zheng Shaowei 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.6

BACKGROUND: Implantation of bone marrow mesenchymal stem cells (BMSCs) is a potential alternative for promoting bone defects healing or osseointegration in osteoporosis. However, the reactive oxygen species (ROS) accumulated and excessive inflammation in the osteoporotic microenvironment could weaken the self-replication and multi-directional differentiation of transplanted BMSCs. METHODS: In this study, to improve the hostile microenvironment in osteoporosis, Poloxamer 407 and hyaluronic acid (HA) was crosslinked to synthetize a thermos-responsive and injectable hydrogel to load MnO2 nanoparticles as a protective carrier (MnO2@Pol/HA hydrogel) for delivering BMSCs. RESULTS: The resulting MnO2@Pol/HA hydrogel processed excellent biocompatibility and durable retention time, and can eliminate accumulated ROS effectively, thereby protecting BMSCs from ROS-mediated inhibition of cell viability, including survival, proliferation, and osteogenic differentiation. In osteoporotic bone defects, implanting of this BMSCs incorporated MnO2@Pol/HA hydrogel significantly eliminated ROS level in bone marrow and bone tissue, induced macrophages polarization from M1 to M2 phenotype, decreased the expression of pro-inflammatory cytokines (e.g., TNFa, IL-1b, and IL-6) and osteogenic related factors (e.g., TGF-b and PDGF). CONCLUSION: This hydrogel-based BMSCs protected delivery strategy indicated better bone repair effect than BMSCs delivering or MnO2@Pol/HA hydrogel implantation singly, which providing a potential alternative strategy for enhancing osteoporotic bone defects healing.

Evaluation of a Novel Array-Based Toxoplasma, Rubella, Cytomegalovirus, and Herpes Simplex Virus IgG Enzyme Linked Immunosorbent Assay and Its Comparison with Virion/Serion Enzyme Linked Immunosorbent Assays

Dongsheng Wu,Yuanjian Wu,Liuhong Wang,Weidong Xu,Qiao Zhong 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.1

Background: The dramatic increase in use of the IgG test for toxoplasma, rubella, cyto- megalovirus (CMV), and herpes simplex virus (HSV) [TORCH] has led to the requirement for a high-efficiency method that can be used in the clinical laboratory. This study aimed to compare the results of BGI-Array ELISA TORCH IgG (BGI-GBI, China) screening method to those of Virion/Serion TORCH IgG ELISA (Virion/Serion, Germany). Methods: Serum specimens (n=400) submitted for routine IgG testing by Virion/Serion ELISA were also tested using the BGI-Array ELISA method. The agreements of these two kinds of method were analyzed by κ-coefficients calculation. Results: Following repeat testing, the BGI-Array ELISA TORCH IgG assays demonstrated agreements of 99.5% (398/400 specimens), 98% (392/400 specimens), 99% (396/400 specimens), and 99.5% (398/400 specimens), respectively. The BGI-Array ELISA IgG as- says provided results comparable to Virion/Serion ELISA results, with κ-coefficients show- ing near-perfect agreement for the HSV (κ=0.87), rubella (κ=0.92) and CMV (κ=0.93) and substantial agreement for the toxoplasma (κ=0.80) IgG assays. The use of the BGI- Array ELISA TORCH IgG assays could reduce the turnaround time (1.5 hr vs. 5 hr by Vi- rion/Serion ELISA for 100 specimens) and were easy to use. Conclusions: BGI-Array ELISA TORCH IgG shows a good agreement with Virion/Serion ELISA methods and is suitable for clinical application.

Clonal Spread of Carbapenem Non-susceptible Acinetobacter baumannii in an Intensive Care Unit in a Teaching Hospital in China

Qiao Zhong,Weidong Xu,Yuanjian Wu,Hongxing Xu 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.6

Background: This study was aimed to investigate the genetic diversity and antibiotic resistance profile of the nosocomial infection agent Acinetobacter baumannii from a medical intensive care unit (ICU) in a teaching hospital in Suzhou, China. Methods: The genetic relationship among A. baumannii isolates in an ICU was investigated using multilocus sequence typing (MLST). The antibiotic resistance pattern was determined by performing an antibiotic susceptible test, which included an agar dilution method and an E-test method. Resistant determinants, e.g., carbapenemase genes, metallo-β- lactamases, and class 1 integron, were analyzed by specific PCR and DNA sequencing. Results: In the present study, 33 non-duplicate isolates were identified as 5 existing sequence types (STs) (ST92, ST75, ST112, ST145, and ST345) and 1 new sequence type STn, which has a G-A mutation at nt268 on ropD40 of ST251. These results reveal limited diversity in carbapenem non-susceptible A. baumannii (CNSAb) isolates in our ICU, which are comprised of only 2 distinct STs, with ST92 and ST75 clustering into a clonal complex (CC) 92. Most CNSAb isolates (94.4%, 17/18) harbored the OXA-23 gene, while no carbapenem-susceptible A. baumannii (CSAb) isolates harbored it. In addition, 66.7% (22/33) isolates were positive for class 1 integrase, and gene cassette analysis showed there are 3 gene arrays among them, i.e., aacA4-catB8-aadA1 (77.3%, 17/22), aacA4 (22.7%, 5/22), and aacC1-orfX-orfX’-aadA1 (4.5%, 1/22). Conclusions: When all these data are combined, the antibiotic resistance and wide distribution of CNSAb isolates in our ICU are probably caused by expansion of the CC92 clone. Background: This study was aimed to investigate the genetic diversity and antibiotic resistance profile of the nosocomial infection agent Acinetobacter baumannii from a medical intensive care unit (ICU) in a teaching hospital in Suzhou, China. Methods: The genetic relationship among A. baumannii isolates in an ICU was investigated using multilocus sequence typing (MLST). The antibiotic resistance pattern was determined by performing an antibiotic susceptible test, which included an agar dilution method and an E-test method. Resistant determinants, e.g., carbapenemase genes, metallo-β- lactamases, and class 1 integron, were analyzed by specific PCR and DNA sequencing. Results: In the present study, 33 non-duplicate isolates were identified as 5 existing sequence types (STs) (ST92, ST75, ST112, ST145, and ST345) and 1 new sequence type STn, which has a G-A mutation at nt268 on ropD40 of ST251. These results reveal limited diversity in carbapenem non-susceptible A. baumannii (CNSAb) isolates in our ICU, which are comprised of only 2 distinct STs, with ST92 and ST75 clustering into a clonal complex (CC) 92. Most CNSAb isolates (94.4%, 17/18) harbored the OXA-23 gene, while no carbapenem-susceptible A. baumannii (CSAb) isolates harbored it. In addition, 66.7% (22/33) isolates were positive for class 1 integrase, and gene cassette analysis showed there are 3 gene arrays among them, i.e., aacA4-catB8-aadA1 (77.3%, 17/22), aacA4 (22.7%, 5/22), and aacC1-orfX-orfX’-aadA1 (4.5%, 1/22). Conclusions: When all these data are combined, the antibiotic resistance and wide distribution of CNSAb isolates in our ICU are probably caused by expansion of the CC92 clone.

WSN Missing Data Imputing Based on Multiple Time Granularity

Jianfeng Xu,Yu Li,Yuanjian Zhang,Azhar Mahmood 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.6

Missing data is a common phenomenon in the data collection process of wireless sensor network (WSN), and the missing data imputing is an important issue of WSN stream data mining. Currently WSN missing data imputing method has little considered about the dynamic characteristics of internal data time structure during the data collection process, which makes data imputing difficult to reflect the real monitoring change objectively. In order to analyze the internal structure and dynamics of WSN time sequence data systematically, with the equivalence relation of the monitored object the time domain can be regarded as a series of integral time granule (ie atomic time point set), a wireless sensor network timing information system (WTIS) is established. The system can reason logically at different time granularity, and a multiple optimal time granularity strategy of WTIS based on hierarchical successive approximation approach is proposed. Finally, based on the research, a multiple optimal time granularity WSN missing data clustering imputing algorithm is proposed. Compared with traditional fixed time granularity missing data imputing algorithm, experiments show that the algorithm can lower error rate when imputing WSN missing data.

Lexical Recoding via Bilingual Memory in Sight Interpreting : A Combined Eye-tracking and Corpus-assisted Study

Yue Lang,Linping Hou,Yuanjian He 이화여자대학교 통역번역연구소 2018 T&I review Vol.8 No.-

This paper reports the results of a triangulated study that combined eye-tracking and corpus-assisted methods on lexical recoding via bilingual memory in sight interpreting of cultural specificity (CF) from English (L2) into Chinese (L1) by student interpreters. The results show that the patterns of eye-tracking and those of the experimental corpus both indicate that CF causes processing load to increase lexical recoding, and the fact that the two patterns converge on one another implies that lexical recoding relies also crucially on bilingual memory. Moreover, we argue that a theoretical element should be an integral part of triangulation. To a larger extent the theory may reflect the underlying bilingual-processing process for translating and interpreting, the more feasible it is in offering a unified account for experimental as well as corpus-extracted data patterns. Further research is needed.

A Novel Dynamic Time Wrapping Similarity Algorithm Optimized by Multi-Granularity

Xu Jianfeng,Tang Tao,Zhang Yuanjian 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.10

Dynamic time warping algorithm (DTW) is a method of measuring the similarity of time series. Concerning the problem that DTW cannot keep high classification accuracy when the computation speed improved, a FG-DTW method based on the idea of naive granular computing is proposed. In this method, firstly, better temporal granularity is acquired by calculating temporal variance feature and it is used to replace original time series; Secondly, the elastic size of under comparing time series granularity allow dynamic adjustment through DTW algorithm and optimal time series corresponding granularity is obtained; Finally, DTW distance is calculated by optimal corresponding granularity model. At the same time, the early termination strategy of infimum function is introduced to improve the efficiency of FG-DTW algorithm. Experiments show that the proposed algorithm improves the running rate and accuracy effectively.

Removal of Cu2+ from aqueous solution by adsorption onto mercapto functionalized palygorskite

Jun Han,Xuefeng Liang,Yingming Xu,Yuanjian Xu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.23 No.-

In order to enhance sorption capacity of palygorskite for heavy metals, mercapto functionalized palygorskite (MPAL) were prepared and utilized for the sorption of Cu2+ from aqueous solution. Sorption kinetic and thermodynamic experiments were conducted to assess the sorption behavior and application potentials for the remediation of heavy-meta-polluted soil. Sorption mechanisms were investigated by XRD, FT-IR, 29Si CP/MAS NMR, SEM-EDS and XPS in detail. The maximum sorption amount of Cu2+ on MPAL was approximately 30 mg/g, which was 10 times more than pristine palygorskite. Freundlich isotherm describes the sorption data better than other isotherms, and pseudo second order kinetic model fit the sorption kinetic processes well. The positive enthalpy change confirmed that the sorption process of MPAL was endothermic and the positive entropy changes revealed that sorption of Cu2+ was driven by entropy changes. Further characterization analyses and the ‘‘Hard and Soft Acids and Bases theory’’ for mechenism research exhibited the complexation existence of Cu2+ with mercapto groups. The good performance demonstrated that MPAL has great potential application values in removal of heavy metals both from aqueous and soils.

Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Yang Sheng,Xie JiaJun,Pan ZhiJie,Guan HongMei,Tu YueSheng,Ye YuanJian,Huang ShouBin,Fu ShiQiang,Li KangXian,Huang ZhiWei,Li XiaoQi,Shi ZhanJun,Li Le,Zhang Yang 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

The meniscus is vital for maintaining knee homeostasis and function. Meniscal calcification is one of the earliest radiological indicators of knee osteoarthritis (KOA), and meniscal calcification is associated with alterations in biomechanical properties. Meniscal calcification originates from a biochemical process similar to vascular calcification. Advanced glycation end products (AGEs) and their receptors (RAGEs) reportedly play critical roles in vascular calcification. Herein, we investigated whether targeting AGE-RAGE is a potential treatment for meniscal calcification. In our study, we demonstrated that AGE-RAGE promotes the osteogenesis of meniscal cells and exacerbates meniscal calcification. Mechanistically, AGE-RAGE activates mTOR and simultaneously promotes ATF4 accumulation, thereby facilitating the ATF4-mTOR positive feedback loop that enhances the osteogenic capacity of meniscal cells. In this regard, mTOR inhibits ATF4 degradation by reducing its ubiquitination, while ATF4 activates mTOR by increasing arginine uptake. Our findings substantiate the unique role of AGE-RAGE in the meniscus and reveal the role of the ATF4-mTOR positive feedback loop during the osteogenesis of meniscal cells; these results provide potential therapeutic targets for KOA.