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        Evaluation of Kinetic Parameters and Mass Transfer of Glucose-fed Granules under Hypoxic Conditions

        Yuancai Chen,Shiyu Fu,Huaiyu Zhan,Che-Jen Lin,Huaixia Lan 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6

        This study demonstrated that the availability of oxygen influenc the kinetic parameters of sludge granules for the utilization and mass transfer of substrates. Batch experiments revealed that substrate utilization of the coupled sludge granules followed Monod’s kinetic model under hypoxic conditions and at initial substrate concentrations ranging from 1,350 to 4,456 mg/L. The corresponding kinetic coefficients of ks (maximum specific substrate glucose utilization rate), Ks (half saturation coefficient),and Y (growth yield) were 5.6 ~ 7.8/day, 58 ~ 64 mg/L, and 0.11 ~ 0.17 mg of MLSS/mg of COD, respectively. Low dissolved oxygen content suppressed the activity of aerobic enzymes, which resulted in a ks value between those of aerobic granules and anaerobic granules. The maximum oxygen consumption rate (ko = 0.89/day) was relatively higher while the half-saturation constant (Ko = 1.71 mg/L)was significantly lower than those of aerobic granules. These results imply that dissolved oxygen was used more efficiently under hypoxic conditions. Thiele modulus (φ)and effectiveness factor (η) analysis revealed that the activity of microorganisms inside the granules was limited by the availability of oxygen. These properties differed from those found in aerobic granules, anaerobic granules,and activated sludge.

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        Effect of Texture and Microstructure on Tensile Behaviors in the Polycrystalline Pure Niobium

        Shanshan Zhang,Yuancai Liu,Tiewei Xu,Mingxue Sun,Qi Zhang,Yong Wan 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.10

        Pure polycrystalline niobium had different texture and microstructure due to the RCD-60, RCD-84 and CD-84 fabricationprocesses and recrystallization at 1000 °C for 2 h. The tensile behaviors and texture evolutions of the niobium were investigatedto reveal the correlation of initial texture/microstructure and tensile properties. The crucial differences of microstructureand orientations after recrystallization would influence tensile properties of the niobium. The differences of tensile behaviorsduring tension testing correspond to the different microstructure and texture evolution in the niobium. The enhanced ⟨110⟩ //RD texture during the tensile deformation indicates that orientations of sub-structures are arranging along the tensile axis inthe RCD-60 niobium. The RCD-84 and CD-84 niobium present the other phenomenon, that sub-structures near the fractureoccur a significant rotation and the ⟨110⟩ // RD texture has been slightly changed after tensile failure. The recrystallizedniobium fabricated by the RCD-84 obtains excellent tensile properties with ultimate tensile strength of 364 MPa and elongationof 55%. The strain hardening rate of the CD-84 niobium is the highest during the initial stage of tensile deformation,and becomes lower than that of the RCD-84 niobium after the 16% strain. Furthermore, the analysis of Taylor factors isapplied to reveal the difference of hardening rates during tensile deformation due to different fabrication and recrystallizationprocesses of the polycrystalline niobium.

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        Kinetics Study of Enzymatic Hydrolysis of Paulownia by Dilute Acid, Alkali, and Ultrasonic-assisted Alkali Pretreatments

        Xiao-kun Ye,Yuancai Chen 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2

        Paulownia, a fast-growing and high-fiber plant (cellulose: 41.66% and hemicellulose: 19.61%), has the potential to serve as an interesting source for production of bioethanol. The aim of this paper is to study and compare the kinetics of enzymatic hydrolysis of Paulownia pretreated by dilute acid (DA), alkali (AL) and ultrasonic-assisted alkali (UA). The enzymatic hydrolysis was performed at 50°C, atmospheric pressure with 40 FPU/g-cellulose cellulase and 80 CBU/g-cellulose cellobiase. The hydrolysis process can be successfully described by the Michaelis-Menten model under the three pretreatment conditions. Due to the high removal of lignin and increased porosity of the substrate, UA pretreatment is proved to be the most effective method in improving enzymatic saccharification, followed by DA pretreatment and alkali (AL) pretreatment. Inhibition constant KI of all experiments (DA: 2.16 g/L, AL: 3.12 g/L and UA: 1.83 g/L) suggests that glucose has a strong inhibition for enzymatic hydrolysis, for lower KI is proportional to higher inhibition performance. The experimental date fits well with kinetics model. This indicates that the model is suitable for performance monitoring, conditions optimization and process control at full-scale studies.

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