Genetic Variants in ASCT2 Gene are Associated with the Prognosis of Transarterial Chemoembolisation-Treated Early-Stage Hepatocelluar Carcinoma

Ge, Nai-Jian,Shi, Zhi-Yong,Yu, Xiao-He,Huang, Xiao-Jun,Wu, You-Sheng,Chen, Yuan-Yuan,Zhang, Jin,Yang, Ye-Fa Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies worldwide. Transarterial chemoembolisation (TACE) is the standardized therapy for intermediate stage HCC. However, the prognosis for HCC patients treated by TACE greatly varies. Thus, there is a critical need for finding biomarkers to predict the prognosis of HCC patients. The amino acid transporter-2 (ASCT2) is involved in tumorigenesis and progression of many malignancies. This study aimed to evaluate the predictive role of two single nuclear polymorphisms (SNPs, rs3826793 and rs2070246) in the ASCT2 gene in HCC patients treated by TACE. Materials and Methods: Two functional SNPs (rs3826793 and rs2070246) in the ASCT2 gene were selected and genotyped using the Sequenom iPLEX genotyping system in a cohort of 448 unresectable Chinese HCC patients treated by TACE. Univariate and multivariate Cox proportional hazards models and Kaplan-Meier curves were used for the prognosis analyses. Results: There was no significant association between two SNPs (rs3826793 and rs2070246) in the ASCT2 gene and overall survival of TACE treated HCC patients. However, we demonstrated that patients with early stage HCC carrying T genotype in rs2070246 showed better OS than those carrying CC genotype (P=0.023). Conclusions: We demonstrated that patients with early stage HCC carrying T genotype in rs2070246 showed better OS than those carrying CC genotype.

Stereo-digital image correlation in the behavior investigation of CFRP-steel composite members

Xiao-Yuan He,Yun-Tong Dai,Hai-Tao Wang,Tian-Yuan Ge,Gang Wu,Jian-Xiao Wan,Shuang-Yin Cao,Fu-Jun Yang 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.23 No.6

The application of carbon fiber reinforced polymer (CFRP) in steel structures primarily includes two categories, i.e., the bond-critical application and the contact-critical application. Debonding failure and buckling failure are the main failure modes for these two applications. Conventional electrometric techniques may not provide precise results because of the limitations associated with single-point contact measurements. A nondestructive full-field measurement technique is a valuable alternative to conventional methods. In this study, the digital image correlation (DIC) technique was adopted to investigate the bond behavior and buckling behavior of CFRP-steel composite members. The CFRP-to-steel bonded joint and the CFRPstrengthened square hollow section (SHS) steel column were tested to verify the suitability of the DIC technique. The stereo- DIC technique was utilized to measure continuous deformation. The bond-slip relationship of the CFRP-to-steel interface was derived using the DIC data. Additionally, a multi-camera DIC system consisting of four stereo-DIC subsystems was proposed and applied to the compressive test of CFRP-strengthened SHS steel column. The precise buckling location and CFRP delamination of the CFRP-strengthened SHS steel column were identified. The experimental results confirm that the stereo-DIC technique can provide effective measurements for investigating the behaviors of CFRP-steel composite members.

Characteristics, Controlled-release and Antimicrobial Properties of Tea Tree Oil Liposomes-incorporated Chitosan-based Electrospun Nanofiber Mats

Yan Ge,Jiapeng Tang,Haihong Fu,Yijun Fu,Yuan-Yuan Wu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

In this paper, a notable chitosan/poly(ethylene oxide) nanofiber mats containing tea tree oil liposomes (TOLCENs)were successfully fabricated using electrospinning process. The microstructures and morphology were characterizedby scanning electron microscopy. The porosity, fluid absorbability, water vapor permeability and mechanical properties ofnanofiber mats were also estimated by ethanol density method, gravimetric method, dish method and tensile test,respectively. Compared to the chitosan/poly(ethylene oxide) composite freeze-dried sponges containing tea tree oilliposomes, TOL-CENs had greater porosity, water absorption, breathability and better mechanical properties. In addition, thecontrolled-release properties and long-term bactericidal capability of the material were also assessed. From the analysis of therelease kinetics and mechanism, it was found that the significant decreased terpinen-4-ol concentration gradient fromliposomal surface to the outside of material was the key to the sustained terpinen-4-ol release in virtue of liposomalencapsulation. TOL-CENs exhibited long-term and more excellent microbicidal effects against Staphylococcus aureus,Escherichia coli and Candida albicans than chitosan/poly(ethylene oxide) nanofiber mats. The combination of tea tree oilliposomes and chitosan in nanofiber mats synergistically destroyed cell membrane, prevented cell adhesion and caused theirregular aggregation of cytoplasm, resulting in cell disintegration observed by transmission electron microscope. Insummary, TOL-CENs had potential application value as a long-term antimicrobial nonwoven materials.

중국 흑룡강성 간흡충증 관리사업(2001-2004)

지주오,이즈민,왕수유,위안런,거타오,위안샹,추이창위안,거홍안,평정,슈롱치,홍성태,최민호,조승열,최동일,홍광선,임한종,이순형,Ji, Zhuo,Li, Zhimin,Wang, Shuyu,Yuan, Ren,Ge, Tao,Yuan, Shang,Cui, Changyuan,Ge, Hongan,Feng, Zheng,Xu, Longqi,Hong, Sung-Tae,Choi, 한국건강관리협회 2005 한국건강관리협회지 Vol.3 No.1

The present pilot project was executed to recommend a strategy of clonorchiasis control in China. The pilot area of this project was Zhaoyuan, Hailin, and Ningan, Heiloagjiang province. A baseline survey subjecting 4,865 residents in Heilongjiang confirmed Zhaoyuan asa high endemic area and Hailin and Ningan as moderate endemic areas. Six different control strategies were implemented in Zhaoyuan, two were in Hailin, and one was in Ningan. Including the baseline survey and project programs from 2000 to 2004, total 63,274subject-times were examined of their feces for Clonorchiseggs, 26,680 were treated, 10,082 were screened by ELISA, and 6,130 subjects were examined of their liver by sonography. The egg Positive rates in 6 villages of Zhaoyuan were as high as 44.8% 70,0%. Following the protocolof each strategy, the subjected residents were examined of their feces and treated with 25 mg/kg praziquantel, 3 times. Except the control group, all of the villages showed 72.8% to 92.0% reduction of their original egg Positive rates at Zhaoyuan. Mass treatments of all subjected residents in 2001 and 2003 reduced the egg rate from 68.8% to18.7% and 4 annual mass treatments reduced the rate from 44.8% in 2001 to 8.7% in 2004.Selective annual treatments of egg positive subjects reduced the egg rates from 50.8% in2001 to 13.8% in 2004 or from 70.0% in 2001 to 11.6% in 2004, and two treatments in a year reduced the rate from 57.6% in 2001 to 4.6% in 2004. According to repeated treatments, EPG counts decreased remarkably. In moderate endemic areas, the original egg rates were 22.6% and 28.3% in 2001 but were 1.7% and 1.1% after 2 or 3 selective treatments. The present findings of the chemotherapeutic control of clonorchiasis prove that repeated medication is important. The reduction is directly correlated with dose of praziquantel but not with mass or selective treatments. Chemotherapeutic control of reservoirhosts has little effect on reinfection of clonorchiasis because the field along the Songhua-jiang is too wide to be impacted. ELISA confirmed many serologically positive cases to Clonorchisantigen but only a few cases were positive to other antigens (Paragonimus, cysticercus, sparganum). The abdominal soaography visualized intrahepatic bile duct dilatation and periductal echo in 2,002 of 6,070 examined subjects. In addition to these examinations and treatment, health education supplemented tㅗe control activities. The present findings prove clonorchiasis is very widely prevalent and heavily endemic along the rivers in Heiloagjiang. The results suggest that group chemotherapy with praziquantel is effective to reduce endemicity of clonorchiasis. Mass treatment without individual fecal examination is recommended in heavy endemic areas where the egg rate is over 40% while one selective treatment is effective enough in moderate endemic areas.

Carotenoid Intake and Esophageal Cancer Risk: a Meta-analysis

Ge, Xiao-Xiao,Xing, Mei-Yuan,Yu, Lan-Fang,Shen, Peng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

This meta-analysis was conducted to evaluate the association between intake of carotenoids and risk of esophageal cancer. A systematic search using PubMed, Cochrane Library, Web of Science, Scopus, CNKI, and CBM (updated to 6 May 2012) identified ten articles meeting the inclusion criteria with 1,958 cases of esophageal cancer and 4,529 controls. Higher intake of beta-carotene, alpha-carotene, lycopene, beta-cryptoxanthin, lutein, and zeaxanthin reduced esophageal cancer risk with pooled ORs of 0.58 (95% CI 0.44, 0.77), 0.81 (95% CI 0.70, 0.94), 0.75 (95% CI 0.64, 0.86), 0.80 (95% CI 0.66, 0.97), and 0.71 (95% CI 0.59, 0.87), respectively. In subgroup analyses, beta-carotene showed protective effects against esophageal adenocarcinoma in studies located in Europe and North America. Alpha-carotene, lycopene, and beta-cryptoxanthin showed protection against esophageal squamous cell cancer. This meta-analysis suggested that higher intake of carotenoids (beta-carotene, alpha-carotene, lycopene, beta-cryptoxanthin, lutein, and zeaxanthin) is associated with lower risk of esophageal cancer. Further research with large-sample studies need to be conducted to better clarify the potentially protective mechanisms of carotenoid associations risk of different types of esophageal cancer.

The domestication event of the Tibetan pig revealed to be in the upstream region of the Yellow River based on the mtDNA D-loop

Ge, Qianyun,Gao, Caixia,Cai, Yuan,Jiao, Ting,Quan, Jinqiang,Guo, Yongbo,Zheng, Wangshan,Zhao, Shengguo Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4

Objective: Evidence from previous reports indicates that pig domestication in East Asia mainly occurred in the Mekong region and the middle and downstream regions of the Yangtze River. Further research identified two new origin centers for domestic pigs in the Tibetan Plateau and the islands of Southeast Asia. However, due to the small sample size of Tibetan pigs, details of the origin and spread of Tibetan pigs has not yet been established. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces. Comprehensive Tibetan pig samples were taken to perform the most detailed analysis of Tibetan pigs to date. Results: The result indicate that Rkaze pigs had the lowest level of diversity, while Changdu pigs had the highest diversity. Interestingly, these two populations were both in the Tibetan Plateau area. If we calculate diversity in terms of each province, the Tibetan Plateau area had the lowest diversity, while the Chinese province of Gansu had the highest diversity. Diversity gradient analysis of major haplotypes suggested three domestication centers of Tibetan pigs in the Tibetan Plateau and the Chinese provinces of Gansu and Yunnan. Conclusion: We found two new domestication centers for Tibetan pigs. One is in the Chinese province of Gansu, which lies in the upstream region of the Yellow River, and the other is in the Chinese province of Yunnan.

Evaluating genetic diversity and identifying priority conservation for seven Tibetan pig populations in China based on the mtDNA D-loop

Ge Qianyun,Gao Caixia,Cai Yuan,Jiao Ting,Quan Jinqiang,Guo Yongbo,Zheng Wangshan,Zhao Shengguo 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

Transcriptome and proteomics-based analysis to investigate the regulatory mechanism of silk gland differences between reciprocal cross silkworm, Bombyx mori

Ge Qi,Xiao Rui,Yuan Yi,He Suqun,Chen Liang,Ma Shangshang,Taha Rehab Hosny,Yao Qin,Chen Keping 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

The silkworm, Bombyx mori, is an important model economic insect. Cross breeding is an important way to select excellent silkworm varieties, and different combinations among the same parents often show different genetic characters. To explore the regulatory mechanism of silk gland differences in reciprocal cross silkworm, the 3rd day of the 5th instar of the orthogonal silkworm (306♀ × NB♂, 798♀ × NB♂) and the reverse cross silkworm (NB♀ × 306♂, NB♀ × 798♂) silk glands were used as experimental materials, then using Label-free-based proteomics and RNA-Seq-based transcriptomics for analysis. Besides, the qRT-PCR was used to validate the expression of differentially expressed genes. The results showed that in NB♀ × 306♂ VS 306♀ × NB♂ group, 280 differentially expressed genes and 238 differential proteins were up-regulated while 40 differentially expressed genes and 149 differential proteins were down-regulated; in NB♀ × 798♂ VS 798♀ × NB♂, 82 differentially expressed genes and 325 differential proteins were up-regulated while 113 differentially expressed genes and 258 differential proteins were down-regulated. Further analysis indicated that the up-regulation of mitochondrial aldehyde dehydrogenase gene and cytochrome P450 gene, which are related to mitochondria, might be regulated by maternal inheritance. The GO and KEGG enrichment initially showed that the differential genes and proteins are mainly involved in processes such as protease metabolism, ATP hydrolysis, lipid transport, insect hormone synthesis, and TCA cycle. The down-regulated expression of ecdysone oxidase and Serpin-32 in the reverse cross group will reduce the energy utilization in the process of silk gland synthesis, increase the hydrolysis of serine, affect the synthesis and transport of silk protein, and cause the difference between reciprocal crosses.

Expression of lncRNA NEAT1 in endometriosis and its biological functions in ectopic endometrial cells as mediated via miR-124-3p

Yuan Donglan,Zhu Dandan,Yin Boyu,Ge Hongshan,Zhao Yinling,Huang Aihua,Wang Xiaosu,Cao Xiuhong,Xia Nan,Qian Hua 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: Endometriosis (EM) is a gynecological disease that poses severe health risks to women, although its pathogenesis has yet to be fully elucidated. It has been shown that long non-coding RNAs (lncRNAs) are closely associated with EM initiation and have a role in the development of this disease. Previous studies exploring the expression of the lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) have shown that this lncRNA functions as a tumor promoter in endometrial cancer. However, its exact mechanism of action in EM remains unclear. Objective: This report was designed to illustrate the potential molecular mechanisms of lncRNA NEAT1 on EM. Methods: Endometrial tissues were extracted from EM model rats and patients with EM. Hematoxylin and eosin staining was applied to detect the morphological changes that occurred in rats after construction of the model. Endometrial stromal cells (ESCs) were extracted from either ectopic endometrium (EC) or eutopic endometrium (EU) tissues from patients with EM. LncRNA NEAT1 and miR-124-3p expression in EM tissues and cells were subsequently evaluated by reverse transcription-quantitative (RT-q)PCR analysis. MTT assay, flow cytometric analysis, western blot assay and Transwell assay were then employed to examine the effect of NEAT1 and miR-124-3p on EC-ESC proliferation, apoptosis, migration and invasion, respectively. The targeted relationship between lncRNA NEAT1 and miR-124-3p was subsequently confirmed by dual-luciferase and co-transfection assays. Results: MiR-124-3p was identified as a target of NEAT1, and could be negatively regulated by NEAT1 in EC-ESCs. The expression level of NEAT1 was evidently increased, whereas that of miR-124-3p was decreased, in the EM in vivo model, EM tissues and EC-ESCs from patients with EM. The loss-of-function assays further established that silencing of NEAT1 could inhibit EC-ESC proliferation, migration, and invasion, but it led to the promotion of apoptosis via targeting miR-124-3p. Conclusions: NEAT1 is significantly upregulated in EM, promoting malignant behavior in EM through targeting miR-124-3p expression.

Microstructure and Magnetic Properties of Fe-doped MnBi Permanent Magnet Alloys

Yuan-Hao Tu,Qiong Wu,Hong-Liang Ge,Zi-Sheng Wang,Min-Xiang Pan,Yu Zhao 한국자기학회 2021 Journal of Magnetics Vol.26 No.2