Suppression of long non-coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis through regulation of MELK targeted by miR-142-5p

Yu‑hua Xie,Jiao Hu 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Glioma has the chief type of primary brain tumors worldwide. The glioma may be controlled by regulatorsincluding some lncRNAs, miRNAs, and proteins. Objective Our study aims to discover the underlying mechanism for lncPCAT19/miR-142-5p/MELK axis in gliomaprogression. Methods The clinical samples were from patients with gliomas in our Hospital. Hematoxylin–eosin staining (H&E) wasapplied to determine the clinical pathological changes. Real time PCR was performed to measure the levels of lncPCAT19,miR-142-5p, MELK, and expression of other genes. Western blot was conducted to detect the protein level of MELK. RIPassay was performed to analyze the interaction between lncPCAT19 and miR-142-5p, and dual-luciferase reporter assay wasused to determine the binding site between lncPCAT19 and miR-142-5p. CCK-8, colony formation assay, flow cytometry,and trans-well assay were carried out to confirm cell proliferation, colony formation, apoptosis, and invasion, respectively. Results LncPCAT19 was increased in cancer tissues. Then, lncPCAT19 could interact with and down-regulate miR-142-5p. Knockdown of lncPCAT19 distinctly inhibited tumor growth in vivo. Interfering lncPCAT19/overexpression of miR-142-5pdecreased glioma cell proliferation, colony formation and invasion, and promoted cell apoptosis by down-regulating expressionof Cyclin B1, CDK2, N-cadherin, Bcl-2, and by up-regulating expression of Bax and E-cadherin. Moreover, overexpressionof lncPCAT19 overturned tumor-suppressing role of miR-142-5p in cells. Additionally, lncPCAT19 and miR-142-5psynergistically regulated expression of MELK. In conclusion, lncPCAT19 enhanced glioma development via increasingMELK by performing as a sponge of miR-142-5p. Conclusions LncPCAT19 promotes glioma progression by sponging miR-142-5p to upregulate MELK levels. Thus, lncPCAT19/miR-142-5p/MELK signaling would be a potential target for glioma treatment.

Honokiol : A Noncompetitive Tyrosinase Inhibitor from Magnoliae Cortex

Yu-Hua Tian,Tai-hyun Kang,Hyun-Chul Kim,Youn-Chul Kim 한국생약학회 2005 Natural Product Sciences Vol.11 No.2

Effect of the neolignans, honokiol (1) and magnolol (2), isolated from Magnoliae Cortex onmushroom tyrosinase activity was investigated in vitro using L-tyrosine as a substrate. Honokiol (1) inhibited2) did notshow tyrosinase inhibitory effect. Honokiol exhibited tyrosinase inhibitory effect with IC50 value of 67.9M, andproved to act as a non-competitive inhibitor by the analysis of Lineweaver-Burk plot.

Influence of Korean Celebrity Endorsement on Chinese Consumers' Purchase Intention towards Fashion Goods

( Yu Hua Cui ),( Yu Ling Bai ) 한국패션비즈니스학회 2020 패션 비즈니스 Vol.24 No.6

Korean celebrity endorsement is a common practice adopted by companies in China. This study assessed the impact of celebrity endorsement (trustworthiness, attractiveness, congruency, and expertise) on Chinese consumers’ intention to purchase clothes endorsed by Korean celebrities. This study used reliability and validity tests and structural equation model analysis to explore the relationship between variables using SPSS 22.0 and AMOS 22.0. This study conducted an online survey (www.sojump.com) from August 10, 2020 to August 20, 2020 and collected 370 respondents. Results show that all constructs of celebrity endorsement positively affected purchase intention and that celebrity expertise had the strongest impact. With respect to the influence of demographic variables on the constructs, gender, age, and education had a significant effect on trustworthiness; gender and age had a significant effect on attractiveness; and income had an effect on purchase intention. Marketing executives should consider using Korean celebrities for different purposes in brand advertising. Particularly, celebrity expertise, such as experience, knowledge, and skills, should receive attention while publicizing to Chinese consumers. This study contributes to the academic and practical implications of Korean celebrity endorsement.

To control floating drug delivery system in a simulated gastric environment by adjusting the Shell layer formulation

Yu-Tung Hsu,Chen-Yu Kao,Ming-Hua Ho,Shiao-Pieng Lee 한국생체재료학회 2021 생체재료학회지 Vol.25 No.4

Background: Gastroretentive drug delivery system (GDDS) are novel systems that have been recently developed for treating stomach diseases. The key function of all GDDS systems is to control the retention time in the stomach. However, research into the bulk density or entanglement of polymers, especially regarding their effects on drug float and release times, is scarce. Methods: In this research, we prepared the floating core-shell beads carrying tetracycline. The ratio of chitosan and xanthan gum in the shell layer was changed to modify polymer compactness. Tetracycline was encapsulated in the alginate core. Results: Using scanning electron microscopy (SEM) techniques, we observed that the shell formulation did not change the bead morphology. The cross-sectional images showed that the beads were highly porous. The interaction between anionic xanthan gum and cationic chitosan made the shell layer dense, resisting to the mass transfer in the shell layer. Due to the high mass transfer resistance to water penetration, the longer float and delivery time were caused by the dense surface of the beads. The cell culture demonstrated that floating core-shell beads were biocompatible. Importantly, the beads with tetracycline showed a significant prolonged anti-bacterial effect. Conclusion: Research results proved that the floating and releasing progress of core-shell beads can be well controlled by adjusting the shell layer formulation that could promote the function of gastroretentive drugs.

Consumer awareness about mask repurchase intention during coronavirus: The case of Chinese sample

Yu Hua Cui 한국의상디자인학회 2021 한국의상디자인학회지 Vol.23 No.2

중국 신시장인 창업판(創業板, GEB) 개설과 중국 사모펀드시장 투자방안

Yu Hua An 한국재무관리학회 2009 財務管理論叢 Vol.15 No.1

Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes

Yu, Qin Ping,Feng, Ding Yuan,He, Xiao Jun,Wu, Fan,Xia, Min Hao,Dong, Tao,Liu, Yi Hua,Tan, Hui Ze,Zou, Shi Geng,Zheng, Tao,Ou, Xian Hua,Zuo, Jian Jun Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.11

Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.

A Pragmatic Cognitive Oriented-Analysis of Long Conversations in Listening Ccomprehension

Yu-hua Gao 한국영어교육연구학회 2012 영어교육연구 Vol.- No.43

This paper aims to take a tentative pragmatic-cognitive approach to the analysis of long conversations in listening comprehension involved in EFL teaching environment. On the philosophical basis of Experiential Realism, it is intended to integrate Relevance Theory (RT), Adaptation Theory (AT) and the tripartite model of situated discourse analysis proposed by Yue-guo Gu to account for English long conversations. What is worth noting here is that the concept of context defined as psychological or mental construct, which consists of ICM and the general background knowledge, serves as the interface for the synthetic study presented here. Meanwhile, topic, precisely the central discourse topic, serves as a desirable starting point for the data analysis. Moreover, the present study mainly follows Ronald Carter and Michael McCarthy’s (2004) description of the generic organization and speech genres in CANCODE, which stands for ‘Cambridge and Nottingham Corpus of Discourse in English’.Moreover, in this paper, we mainly focus on the dynamic generation of meaning, the dynamic notion of context as well as the integrated progress of linguistic choices and contextualcorrelates. Furthermore, the argumentation is mainly done from the three aspects as follows: goal development analysis, talk exchange development analysis, and interpersonal management analysis. As for the latter, the author will mainly concentrate on strategies involved in goal development and interpersonal management, both of which are realized in talk exchange development. The author highlights such strategies as indirectness, politeness, power, metaphor, topic progression, the activation of ICM and encyclopedia knowledge etc. The major findings of the study are as follows: To start with, it is proved that, in such highly institutionalized social situations as a library, a TV studio, or a workplace, both the speaker and the hearer tend to pursue the institutionalized goals by virtue of the roles they play. Secondly, it is crucial for the students to identify the central discourse topic as well as the topic progression strategies at the initial stage of the on-going process of a particular long conversation. Thirdly, activation of the corresponding ICM and the general background knowledge in a particular conversation can help a lot to bridge the gap caused by the cultural disparity between the students’ cognitive environment and that of the interactants in the target culture. Accordingly, they are able to fill in the information slot and thus successful comprehension will be ensured. Moreover, various strategies are involved in the goal development and interpersonal management, which are goal-oriented and genre-specific. However, some strategies overlap. Furthermore, when status relations, affective involvement, familiarity come into the picture, it makes the matter even worse and far more complicated. The author also explores its implications for EFL teaching from two perspectives: meta-pragmatic awareness and cognitive environment.

Identification and Characterization of the Antifungal Substances of a Novel Streptomyces cavourensis NA4s

( Hua Qi Pan ),( Su Ya Yu ),( Chun Feng Song ),( Nan Wang ),( Hui Ming Hua ),( Jiang Chun Hu ),( Shu Jin Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soilborne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological, and phylogenetic analyses based on its 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and a broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soilborne fungal diseases of plants.

Performance Analysis of a Hydrodynamic Separator for Treating Particulate Pollutants in Highway Rainfall Runoff

Yu Jiang Hua,Qi Tao Yi,Young Chul Kim 대한환경공학회 2009 Environmental Engineering Research Vol.14 No.4

This study examined the separation characteristics of particles in the rainfall runoff from paved roads using a φ7.5 cm hydrocyclone. The volume fraction and total suspended solids concentrations in the overflow and underflow from the hydrocyclone, as well as the separation efficiency were determined. The results indicated that the overflow volume increased with increasing operational pressure, but decreased with decreasing ratio of underflow outlet to inlet sizes (Du/Di), while the underflow to overflow volumes showed contrary behavior. The total suspended solid (TSS) concentration ratio between the overflow and inflow (TSSover/in) decreased as a function of the operational pressure, while the corresponding ratio of underflow to inflow (TSSunder/in) increased. There was no visible difference in the TSSover/in with increasing Du/Di ratio, but the TSSunder/in decreased sharply. The particle removal efficiency was mainly affected by the particle size.