우리나라에서 分離된 살모넬라菌群의 同定에 關한 報告 : 1967-1969

金英子,趙敏基,柳榮海 國立保健硏究院 1970 국립보건연구원보 Vol.7 No.-

Impact of White Matter Changes on Activities of Daily Living in Mild to Moderate Dementia

Moon, So Young,Na, Duk L.,Seo, Sang Won,Lee, Jun-Young,Ku, Bon D.,Kim, Seong Yoon,Park, Kyung Won,Shim, Yong S.,Youn, Young Chul,Chung, Chan-Seung,Cheong, Hae-Kwan,Choi, Seong Hye,Cha, Kyung Ryeol,Kim S. Karger AG 2011 European neurology Vol.65 No.4

<P>The association between white matter changes and activities of daily living (ADL) in a large, well-defined cohort of patients with mild-to-moderate dementia (either Alzheimer’s disease or subcortical vascular dementia) were investigated. A total of 289 patients were divided into three groups (140 mild, 99 moderate, and 50 severe) depending on the degree of white matter changes as indicated on brain magnetic resonance image scans. Further, we analyzed the three groups’ performances on basic and instrumental ADL. The degree of white matter changes was associated with greater age, hypertension, previous history of stroke, higher Hachinski Ischemic Score, worse global cognitive and functional status, and an increased impairment of basic ADL and instrumental ADL. The increased impairment with regard to the severe group’s performance on both the basic and instrumental ADL remained significant after adjustment for age and hypertension. Tasks involving physical activities were most significant. This was the first study investigating the association between white matter changes and ADL in a large, well-defined dementia cohort. The present study suggests that severe white matter changes may be associated with higher impairment on both basic and instrumental ADL.</P><P>Copyright © 2010 S. Karger AG, Basel</P>

왕겨에 의한 신령버섯균사체 액체배양액의 생쥐 항복수암성 증가

Young S. Kim(김영숙),Wook J. Jang(장욱진),Md. A. Rakib(라키브),Jung M. Kwon(권정민),Chae R. Ahn(안채린),So Y. Kim(김소영),Yong U. Cho(조용운),Young K. Ha(하영권),Jeong O. Kim(김정옥),Yeong L. Ha(하영래) 한국생명과학회 2010 생명과학회지 Vol.20 No.9

왕겨가 Agaricus blazei Murill (AB: 신령버섯)균사체 액체배양 추출물의 항암성을 증가시키는지에 관한 연구를 수행하였다. AB균사체를 대두박을 기본으로 한 액체배지에 다양한 조건으로 배양하여, β-glucan 함량, Brix, 균사체를 측정하여 적정 생육조건을 선정하고, 이들의 열수추출물의 S-180 cell로 유도한 mouse 복수암에 대한 항암성을 조사하였다. AB균사체는 25℃에서 5일간 배양하였을 때 최적 생육을 나타내었고, 이 배양물이 다른 조건에서 배양한 배양물보다 우수한 항암성을 나타내었다. AB균사체의 생육 및 항암성은 변온배양에 따른 효과는 없었다. 따라서 이 최적배양조건(25℃, 5일 배양)에서 AB균사체를 1% 왕겨분말이 함유된 액체배지에 배양하고, 이의 열수 추출물의 항암성을 검증하였다. 1% 왕겨가 함유된 액체배지에서 배양한 열수추출물의 항암성은 왕겨가 함유되지 않은 배지의 열수추출물보다 항암성이 유의성 있게 증가되었다(p<0.05). 왕겨의 첨가는 AB균사체의 생육을 오히려 촉진시켰다. 이 결과는 왕겨가 AB균사체 뿐 만 아니라 다른 버섯균사체 액체배양물의 항암성 증진을 위한 원료로 활용될 수 있을 것임을 의미한다. The effects of rice hull (RH) powder on the anticarcinogenic activity of submerged-liquid cultures of Agaricus blazei Murill (AB) were assessed for mouse ascites cancers induced by mouse Sarcoma S-180 (S-180) cancer cells. Optimal growth of AB mycelia in the basal liquid culture medium, containing soybean meal, was achieved by culturing at 25℃ for 5 days, when evaluated by β-glucan content, Brix, and mycelial weight, relative to other culture conditions. Hot-water extract (HWE) of the submergedliquid culture of AB mycelia grown at 25℃ for 5 days exhibited a stronger anticarcinogenic activity, relative to HWE from other culture conditions. No such effects were obtained from AB mycelial cultures by alternative temperature-controlling cultures. Both cytotoxicity for S-180 cells and anticarcinogenic potentials for mouse ascites cancer of the HWE from AB mycelia grown in the basal medium containing 1% RH powder for 5 days at 25℃ were significantly (p<0.05) enhanced, relative to HWE from the AB mycelia culture of the basal medium without RH powder. These results indicate that HWE of submerged-liquid culture of AB mycelia, incubated in media containing 1% RH powder at 25℃ for 5 days, enhanced anticarcinogenic activity against S-180 cell-induced mouse ascites cancer, and suggest that RH powder is an excellent ingredient for the improvement of the anticarcinogenic potentials of the submerged-liquid culture of mushroom mycelia.

살모넬라의 Fimbrial Adhesion Group에 포함되는 Type 1 Fimbriae 유전자 분석

나훈택 ( Hun-taek Na ),정맹식 ( Maeng Sig Joung ),김홍선 ( Hong S. Kim ),이시경 ( Si Kyung Lee ),홍지애 ( J. A. Hong ),김수영 ( Soo Young Kim ) 한국자연치유학회 2017 Journal of Naturopathy Vol.6 No.1-2

본 연구에서는 살모넬라 표준균주 4종과 분리균주 2종을 대상으로 type 1 fimbriae 유전자 fimC, fimD, fimI, fimZ를 각각 비교한 결과 많은 아미노산 변이가 관찰되었다. S. typhimurium ATCC 14028 균주에서 가장 많은 14개의 아미노산 변이가 관찰되었다. 반면 S. typhimurium ATCC 13311 균주에서는 가장 적은 2개의 아미노산 변이가 관찰되었다. 그중에서 유전자 fimC와 fimZ에서는 아미노산 변이가 관찰되지 않았고, fimD와 fimI 유전자에서 Leu527→Pro, Ile130→Val으로 각각 아미노산 변이가 관찰되었다. In this study, a number of amino acid mutations were observed in the type 1 fimbriae genes fimC, fimD, fimI, and fimZ in four Salmonella reference strains and two isolates. S. typhimurium ATCC 14028 strain showed most numerous 14 amino acid variations. On the other hand S. typhimurium ATCC 13311 strain, the least two amino acid variants were observed. Among them, amino acid mutations were not observed in the genes fimC and fimZ, and in the fimD and fimI genes, amino acid variations were observed as Leu527→Pro, Ile130→Val.

Cloning and expression patterns of 14-3-3ζ from the beet armyworm, Spodoptera exigua

Mi Young Noh,Yong Hun Jo,Cheol Hong Park,Young S. Hong,San Sun Kang,Yong Seok Lee,Iksoo Kim,Namjung Kim,Seongon Kim,Jong Dae Park,Yeon Soo Han 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

14-3-3 proteins are known to play a pivotal role in a diverse array of cellular events such as cell survival, apoptosis, and signal transduction. Numerous 14-3-3 ζ have been cloned and characterized from a host of eukaryotic organisms including human, plants, yeast, fruit fly and silkworm. However, no study on Spodoptera exigua 14-3-3ζ in conjunction with virus infection has so far been reported in insects. It appears that expression of Se14-3-3ζ was decreased starting 24 h post-SeNPV infection as SeNPV titers seemed to increase as evidenced by intense bands of SeNPV IAP3. Interestingly, confocal microscopic analysis revealed that Se14-3-3ζ is expressed at the apical side of the NPV-uninfected gut cells, whereas it was detected mainly in the nucleus of the NPV-infected cells. Thus, despite the biological significance of Se14-3-3ζ in S. exigua in conjunction with molecular interactions between SeNPV and S. exigua is unclear now, our data suggest that Se14-3-3 ζ protein plays a role to protect S. exigua from the infection or inhibit replication of SeNPV.

Ribosomal Protein S6, a Target of Rapamycin, Is Involved in the Regulation of rRNA Genes by Possible Epigenetic Changes in <i>Arabidopsis</i>

Kim, Yun-Kyoung,Kim, Sunghan,Shin, Yun-jeong,Hur, Yoon-Sun,Kim, Woo-Young,Lee, Myung-Sok,Cheon, Choong-Ill,Verma, Desh Pal S. American Society for Biochemistry and Molecular Bi 2014 The Journal of biological chemistry Vol.289 No.7

<P>The target of rapamycin (TOR) kinase pathway regulates various biological processes, including translation, synthesis of ribosomal proteins, and transcription of rRNA. The ribosomal protein S6 (RPS6) is one of the well known downstream components of the TOR pathway. Ribosomal proteins have been known to have diverse functions in regulating cellular metabolism as well as protein synthesis. So far, however, little is known about other possible role(s) of RPS6 in plants, besides being a component of the 40 S ribosomal subunit and acting as a target of TOR. Here, we report that RPS6 may have a novel function via interaction with histone deacetylase 2B (AtHD2B) that belongs to the plant-specific histone deacetylase HD2 family. RPS6 and AtHD2B were localized to the nucleolus. Co-expression of RPS6 and AtHD2B caused a change in the location of both RPS6 and AtHD2B to one or several nucleolar spots. ChIP analysis suggests that RPS6 directly interacts with the rRNA gene promoter. Protoplasts overexpressing both <I>AtHD2B</I> and <I>RPS6</I> exhibited down-regulation of pre-18 S rRNA synthesis with a concomitant decrease in transcription of some of the ribosomal proteins, suggesting their direct role in ribosome biogenesis and plant development. This is consistent with the mutation in <I>rps6b</I> that results in reduction in 18 S rRNA transcription and decreased root growth. We propose that the interaction between RPS6 and AtHD2B brings about a change in the chromatin structure of rDNA and thus plays an important role in linking TOR signaling to rDNA transcription and ribosome biogenesis in plants.</P>

Preparation and Characterization of Sulfonated Poly(ether Sulfone) Using 4,4-Bis(4-Hydroxylphenyl)valeric Acid for Proton Exchange Membrane Fuel Cell Application

Seo, Dong Wan,Lim, Young Don,Islam Mollah, M. S.,Lee, Soon Ho,Moon, Sang Ho,Pyun, Sang Young,Kim, Whan Gi Trans Tech Publications, Ltd. 2009 Materials science forum Vol.620 No.-

<P>Poly(ether sulfone)s (PES) containing 25-75 mol % valeric acid were prepared with bisphenol A, bis(4-chlorophenyl)sulfone and 4,4-Bis(4-hydroxylphenyl)valeric acid using potassium carbonate in DMAc (dimethylacetamide) at 160 °C. Copolymers containing carboxylacid group were reduced to hydroxy group by BH3 solution 1M in THF and NaBH4 co-catalyst. Sulfonated poly(ether sulfone)s (S-PES) were obtained by reaction of 1,3-propanesultone and the reduced copolymer (PES-OH) with potassium t-butoxide. A series of copolymers were studied by 1H-NMR spectroscopy, differential scanning calorimetry (DSC), and thermo gravimetric analysis (TGA). Sorption experiments were conducted to observe the interaction of sulfonated polymers with water and methanol. The S-PES membranes exhibited proton conductivities from 1.20  10-3 to 3.40  10-3 S/cm, water swell from 12.25 to 31.50 %, IEC from 0.43 to 0.72 meq/g and methanol diffusion coefficients from 3.60  10-7 to 4.90  10-7 cm2/S at 25 °C.</P>

Nonplanar structure of C₆H₅SCF₃ facilitates πσ^∗-mediated photodissociation reaction on the S₁ state

Kim, So-Yeon,Lee, Jeongmook,Kim, Sang Kyu,Choi, Young S. North Holland 2016 Chemical physics letters Vol.659 No.-

<P>Vibrational structure of trifluoromethylthiobenzene (C6H5SCF3) on the S-1 state has been investigated by resonance-enhanced two-photon ionization spectroscopy and nature of predissociation dynamics is inferred from homogeneously broadened spectral features. As C6H5SCF3 adopts a nonplanar structure in both the S-0 and S-1 states, the effective adiabatic barrier generated by avoided crossing of optically bright bound S-1 (pi pi*) and dark-repulsive Sy (pi sigma*) surfaces along the reaction coordinate is significantly lowered, giving the S-1 lifetime of similar to 300 fs. This experiment demonstrates that the molecular structure spanned by the reactive flux near the curve-crossing region dictates reaction rate as well as nonadiabatic transition probability. (C) 2016 Elsevier B.V. All rights reserved.</P>

Personalized Skin Care Solution from Facial Analysis to Treatment

( Jeong-gun Lee ),( Shin Hee Cho ),( Bo Young Shin ),( Minseok S. Kim ),( Jung-hwan Park ) 한국피부장벽학회 2017 한국피부장벽학회지 Vol.19 No.1

Global home beauty market is booming at an average annual rate of 17% over US 4.1 Billion dollars. Because Steady and professional skin care is very important to maintain good skin condition. S-Skin`s goal is to prepare for the smart beauty era by combining traditional beauty technologies with IT technologies. S-Skin`s solution consists of 3 components. First, S-Skin beauty device that can measure skin condition and LED therapy automatically, General beauty devices on the market have only measurement function or care function, no both, Whereas S-Skin beauty device is an all-in-one type. Our beauty device is based on acquiring accurate skin information from skin measurement and providing skin care based on it. Second, micro needle patches that deliver the active ingredient deep into the skin, Also by using the patch material that changes color with body temperature makes it easy to visually check whether or not it is attached correctly. Further, with the smartphone, when tagging the NFC sticker on the patch or product package. the tagged date is displayed in the app`s calendar to easily manage the patches` frequency of usage. Finally, it consists of an app that recommends skin care method. It then recommends a suitable microneedle patch and LED light care for better skin. Microneedle patches, portable skin measurement and treatment device and smartphone application all working together to create professional-quality skin care solutions.

Phenotypic characteristics of<i>Streptococcus iniae</i>and<i>Streptococcus parauberis</i>isolated from olive flounder (<i>Paralichthys olivaceus</i>)

Nho, Seong-Won,Shin, Gee-Wook,Park, Seong-Bin,Jang, Ho-Bin,Cha, In-Seok,Ha, Mi-Ae,Kim, Young-Rim,Park, Yon-Kyoung,Dalvi, Rishikesh S.,Kang, Bong-Jo,Joh, Seong-Joon,Jung, Tae-Sung Oxford University Press 2009 FEMS microbiology letters Vol.293 No.1

<P>The etiological agents of streptococcosis were isolated from diseased olive flounder collected on the Jeju island of Korea. A total of 151 bacterial isolates were collected between 2003 and 2006. The isolates were examined using various phenotypic and proteomic analyses, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting, and glycoprotein assays. In addition, isolates were grown on blood agar to assess hemolytic activity, and biochemical assays were performed using the API20 Strep kit. Our results revealed that all isolates were nonmotile, Gram-positive cocci that displayed negative catalase and oxidase activities. Multiplex PCR assays revealed that 43% and 57% of the isolates were Streptococcus iniae and Streptococcus parauberis, respectively. These results were consistent with those of the SDS-PAGE and immunoblot analyses using whole-cell lysates of bacterial isolates. Significant differences were observed with respect to the Voges-Proskauer, pyrrodonyl arylamidase, alkaline phosphatase, and hemolytic activities of the S. iniae and S. parauberis isolates. Isolates of S. iniae displayed uniform profiles in the immunoblot and glycoprotein assays; however, immunoblot assays of S. parauberis isolates (using a chicken IgY antibody raised against a homologous isolate) revealed three distinct antigenic profiles. Our findings suggest that S. parauberis and S. iniae are endemic pathogens responsible for the development of streptococcosis in olive flounder.</P>