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Haemophilus influenzae 티올 과산화효소의 특성
황영선(Young Sun Hwang),김강화(Kanghwa Kim) 전남대학교 생활과학연구소 2000 生活科學硏究 Vol.10 No.-
Haemophilus influenzae HI0571 protein that shows homology to E. coli thiol peroxidase uses yeast thioredoxin system as an electron donor[Hwang. Y. S., Chae. H. Z., and Kim. K. (2000) J. Biochem. Mol. Biol. 33, 514~518]. The microorganism has two open, reading frames. HI1158 and HI0084, showing homology to a thioredoxin reductase and a thioredoxin, respectively. Both proteins consisting thioredoxin system were expressed in E. coli, and then the proteins were subsequently purified. The H. influenzae thioredoxin system showed 5.5’-dithiobis-(2-nitrobenzoic acid) reduction activity. H, influenzae HI0751 protein, thiol peroxidase was characterized using the thioredoxin system as an electron donor. The thiol peroxidase showed the thioredoxin dependent peroxidase activity. And glutamine synthetase protection activity of thiol peroxidase against Thiol/Fe<sup>3+</sup>/O₂ system was enhanced by addition of the thioredoxin. An antioxidant function of thiol peroxidase in intact cells was demonstrated by the observation that E. coli cells overexpressed with H. influenzae thiol peroxidase were less sensitive to growth inhibition by alkyl hydroperoxides.
분말형 프로바이오틱스 섭취방법에 따른 구강 내 pH 변화
황영선 ( Young-sun Hwang ),이민경 ( Min-kyung Lee ),김명희 ( Myoung-hee Kim ) 한국치위생학회(구 한국치위생교육학회) 2019 한국치위생학회지 Vol.19 No.6
Objectives: The purpose of this study was to investigate the changes in pH in the oral cavity using the probiotic intake method. Methods: A total of 109 participants were enrolled and randomly assigned to three groups. Participants in the control group did not ingest powdered probiotics, those in experimental group 1 ingested powdered probiotics by dissolving them on the tongue, and those in experimental group 2 dissolved powdered probiotics on the tongue and rinsed with water. pH values were measured 5 times in all. The significance of each group was examined by the Kruskal-Wallis test. The trend over time was expressed as a graph with groupwise means and confidence intervals, considering repeated measurement data. Results: A significant difference was found between the control group and experimental group 1 at two time-points, i.e., immediately after intake and 3 min after ingestion. As a result of the time trend, the pH value of experimental group 2 was smaller than that of experimental group 1, compared to the control group. Conclusions: Studies have shown that taking probiotics with water may help reduce changes in oral pH. Probiotics should be aware of live bacteria and provide consumers with more detailed information on proper dosage and precautions.
황영선 ( Young-sun Hwang ),김기쁨 ( Gi-ppeum Kim ),안경근 ( Kyung-geun Ahn ),김선태 ( Sun Tae Kim ),윤원병 ( Won Byong Yon ),한원영 ( Won Young Han ),정명근 ( Myoung-gun Choung ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-
The distribution and variation of the contents of targeted metabolites in soybean [Glycine max (L.) Merr.] seeds are affected by both genetic and environmental factors. In this study, the 192 soybean germplasm accessions collected from two provinces of Korea to elucidate the effects of seed coat color and 100 seeds dry weight on the metabolic variation and responses of targeted metabolites. The main and interaction effects of seed coat color and 100 seeds dry weight were present in sucrose, total oligosaccharides, total carbohydrates and all measured fatty acids. The targeted metabolites were clustered within three groups, 1) daidzein and genistein, 2) glycitein, protein, and linoleic acid, and 3) the rest of the targeted metabolites. These metabolites were not only differently related to 100 seeds dry weight, based on the difference of seed coat color by regression analyses but also responded differentially to seed coat color in principle component analysis(PCA) loading plots. The inter- relationship between the targeted metabolites was highly present in the result of correlation analysis. Overall, results revealed that the targeted metabolites were diverged in relation to seed coat color and 100 seeds dry weight within locally collected soybean seed germplasm accessions.
Transcription and Post-Transcription Regulation of Primordial Germ Cell in Chicken
Young Sun Hwang(황영선),Sang In Lee,Bo Ram Lee,Sujung Kim,Deivendran Rengaraj,Min Yoo,Kyung Je Park,Gwonhwa Song,Jae Yong Han 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-
초기 생식세포 발달은 전사와 전사 후 조절에 의하여 복잡하게 이루어진다. 따라서 mRNA와 miRNA는 발달에 중요한 역할을 할 것이다. 하지만 miRNA가 어떻게 발달과정에 관여하는지는 많이 알려져 있지 않다. 이 연구에서 닭의 6일령 생식선으로부터 분리한 원시생식세포(PGCs)와 생식선 기질세포(GSCs)로부터 RNA를 얻어 microarray 분석을 하였다. 이를 토대로 특정 miRNA가 원시 생식세포 특이적으로 발현하는 것을 알아냈고, 또한 이 miRNA가 표적 mRNA의 3' 비번역영역(Untranslated reigon)에 결합하여 유전자의 발현을 억제한다는 것을 확인하였다. 이러한 결과는 원시생식세포 특이적 mRNA나 miRNA가 발생에 중요한 역할을 하며 이를 이해하는데 기여할 것이다.
고속액체크로마토그래피를 이용한 비타민 B<SUB>5</SUB> 및 B<SUB>6</SUB>의 정량 분석
김기쁨(Gi-Ppeum Kim),황영선(Young-Sun Hwang),정명근(Myoung-Gun Choung) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.10
식품 함유 비타민 B<SUP>5</SUP> 및 B<SUP>6</SUP>의 최적 HPLC 분석 조건을 검토한 결과 비타민 B<SUP>5</SUP>의 경우 YMC-Pack ODS-AM(250×4.6 mm I.D.) 칼럼을 이용하고, A용매로 50 mM KH₂PO₄(pH 3.5)을, B용매는 아세토니트릴을 이동상 용매로 사용하는 A용매 95% 등용매용리 조건에서 200 nm의 파장으로 분석하는 HPLC/DAD법을 최적조건으로 확립하였다. 한편 비타민 B6의 최적분석조건은 여기파장(excitation) 290 nm, 방출파장(emission) 396 nm로 분석하는 HPLC/FLD법으로써, 칼럼은 YMC-Pack Pro RS C18(250×4.6 mm I.D.), 이 동상 용매는 A용매 20 mM CH₃CO₂Na(pH 3.6), B용매 아세토니트릴을 A용매 97% 등용매용리 조건으로 사용하였다. 비타민 B5 및 B6의 표준검량선은 R<SUP>2</SUP>값이 각각 0.9998 및 0.9999로 고도의 직선성을 나타내었고, 검출한계 및 정량한계는 비타민 B5의 경우 각각 0.4 mg/L 및 1.3 mg/L, 비타민 B6의 경우 각각 0.006 mg/L 및 0.02 mg/L로 산출되었다. Recently, many people have demanded reliable nutritional data even for minor-components. On the other hand, an analytical method for the analyses of vitamin B5 and B6 is lacking. Therefore, this study attempted to validate with accuracy and precision the analysis of vitamin B5 and B6 using a high-performance liquid chromatography (HPLC) method. The vitamin B5 and B6 contents were analyzed using an Agilent 1260 series HPLC system. YMC-Pack ODS-AM (250×4.6 mm I.D.) and YMC-Pack Pro RS C18 (250×4.6 mm I.D.) columns were used for the analyses of vitamin B5 and B6, respectively. In the case of vitamin B5, the flow rate was set to 1.0 mL/min by isocratic elution using the 50 mM KH2PO4 solution (pH 3.5)/acetonitrile (ACN) (95:5, v/v) with monitoring at 200 nm using HPLC/DAD, whereas the flow rate for vitamin B6 was set to 1.0 mL/min of flow rate by isocratic elution using a 20 mM CH₃CO₂Na solution (pH 3.6)/ACN (97:3, v/v) with monitoring by excitation at 290 nm and emission at 396 nm using HPLC/FLD. The column temperature was set to 30°C. The injection volume was 20 μL for each experiment. The specificity of the accuracy and precision for vitamin B5 and B6 were also validated by HPLC. The results showed high linearity in the calibration curve for vitamin B5 (R<SUP>2</SUP>=0.9998**), the limit of detection (LOD) and limit of quantitation (LOQ) were 0.4 mg/L and 1.3 mg/L, respectively, In contrast, for the calibration curve of vitamin B6, which showed high linearity (R<SUP>2</SUP>=0.9999**), the LOD and LOQ were 0.006 mg/L and 0.02 mg/L, respectively.
증기화광산란 검출기를 이용한 콩 함유 수용성 탄수화물의 분석
김경하(Gyeong-Ha Kim),황영선(Young-Sun Hwang),안경근(Kyung-Geun Ahn),김기쁨(Gi-Ppeum Kim),김민지(Min-Ji Kim),홍승범(Seung-Beom Hong),문중경(Jung-Kyeong Moon),정명근(Myoung-Gun Choung) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.7
In the present study, a new analytical method was devised for the simultaneous determination of soluble carbohydrates in soybean seeds using high performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD). The limit of quantification (LOQ) for soybean soluble carbohydrates ranged from 5.6~7.6 mg/kg using the HPLC/ELSD method and from 16.2~33.9 mg/kg using the high performance liquid chromatography/refractive index detection (HPLC/RID) method. Therefore, the HPLC/ELSD method was more sensitive than HPLC/RID. The precision values for retention time and peak area of the HPLC/ELSD method were evaluated by inter-day (n=5) and intra-day (n=10) assays using a standard solution. All precision values (CV<2.5%) for soybean soluble carbohydrates were acceptable and fulfilled international acceptance criteria. All linear calibration curves were obtained with a correlation coefficient of R<SUP>2</SUP>>0.999. The contents of soluble carbohydrates for the "Shingikong" (yellow soybean) and "Cheongjakong 3" (black soybean) samples were analyzed using the HPLC/RID and HPLC/ELSD methods. The difference in carbohydrate contents between the two detection methods was significant. Carbohydrate contents in the HPLC/ELSD method were higher than those in the HPLC/RID method. Overall, the HPLC/ELSD method showed satisfactory resolution with a favorable LOQ and reproducibility. Therefore, these results indicate that the HPLC/ELSD method may be applied to determine the contents of soluble carbohydrates in soybean seeds and related food stuffs.