치성 낭종의 임상-병리학적 소견과 TGF-α 및 TGF-β 발현

한경민,김용환,박진혁,윤형기,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Periapical cyst is a relatively common disease but heterogenous in histogenesis, and inflammatory reaction and fibrosis of varying degree are associated in the cystic wall. The aim of thes study is to evaluate the clinico-pathologic characteristics such as patient's age, ses, location, and the degree of inflammatory reaction and fibrosis of periapical cyst and the role of TGF-α and TGF-β cytokines in the inflammed periapical cyst. Methods and Material : The materials were 43 cases of periapical cyst and clinical information with age, sex, location(maxilla vs mandible, right vs left, region involved) were recorded. Histopathological examination on the degree of inflammatory reaction and fibrosis and immunohistochemical stining for TGF-α and TGF-β were perfomed. Satistical analyses between clinical parameters, the degree of inflammation and fibrosis and expressions for TGF-α and TGF-β were followed. Results : 1. Thirt(69.8%) of 43 cases were older than 20 year-old. and no significant differences of incidence according to patient's sex was noted. In 25(58.1%) out of 43 case. 20(54.1%) out of 37 cases, and 20(48.8%) of 41cases, the cyst was located on the maxilla. in right side and in premolar region respectively. 2. The cysts of developmental and inflammatory origin were 24(55.8%) and 19(44.2%) of 43 cases, respectively and there were no siginificant differences of patient's age, sex, and location of cysts according to the origin of cysts. 3. Fibrosis was more conspicuous in cases older than 20 year-old. (p=0.0179), but no significant difference in the degree of inflammatory reaction according to patient's age and in the degree of inflammatory reaction and fibrosis according to sex and location of the cysts were seen. 4. Inflammatory reaction was slightly more in cysts of inflammatory origin, but no significant defferences in degree of inflammation and fibrosis according to origin of the cysts. 5. The degree of inflammatory reaction and TGF-α and TGF-β expressions showed a tendency of positive correlation(p=0.1563), but there were no statistically significant relationships between the degree of inflammation and fibrosis and TGF-α and TGF-β expressions. 6. In the cysts of developmental origin, TGF-α expression in the epithelial cells was significantly increased than inflammatory origin, but TGF-α and TGF-β expressions in the inflammatory cells and stromal cells showed no significant difference according to the origin Conclusion : The degree of inflammatory reaction and fibrosis of periapical cysts was different according to patient's age and location of the cyst. TGF-α and TGF-β cystokines seemed to be involved in the inflammatory reaction and fibrosis of periapical cyst, however, TGF-α and TGF-β expressions was not parallel to the degree of inflammatory reaction and fibrosis. These results suggest that other cytokines in addition to TGF-α and TGF-β may also be involved in the inflammatory reaction and fibrosis of periapical cyst. Increased TGF-expression in the epithelial cells of the cysts of developmental origin could be a helpful finding to defferentiate the origin of the periapical cyst.

X-線 照射에 의한 小腸粘膜의 走査電子顯微鏡像

김용가,손경락,손태중 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

저자들은 전신 방사선 조사후 소장의 점막 변화를 주사전자 현미경으로 관찰하여, 급성 방사선 상해의 일단을 알아보기 위해, 흰토끼에 2,000rad를 1회 전신 조사하여 말단회장의 절편을 채취하여 시료로 사용하였다. 융모의 변화는 방사선 조사후 20분 군에서 가장 심하게 나타났으며, 이후 시간이 경과함에 따라 융모의 변화는 경미해 지면서 1일후는 거의 정상 융모의 모양을 나타내었으며, 미융모의 크기 및 분포는 시간의 경과와 관계없이 대체로 일정하였으나 3시간 군까지는 미융모의 일부에서 수포형성이 관찰되었다. 미융모의 밀도는 대조군보다는 방사선 상해를 받은 군에서 더욱 높게 나타났다. 개개 세포는 1시간 군까지 부분적으로 세포의 탈락이 있을 뿐 시간경과에 따른 유의성있는 변화는 관찰되지 않았다. 이상의 검사성적으로 미루어 다음과 같은 결론을 얻었다. 첫째,전신 방사선 조사시 상해는 20분 군에서 가장 심하게 나타났으며, 둘째, 융모의 모양은 방사선 조사후 1일 군부터 거의 대조군과 가까운 소견을 보인 것으로 미루어 상피세포의 재생이 왕성하다고 생각된다. 셋째, 편평한 융모나 거대세포는 관찰되지 않았다. 넷째, 미융모는 방사선 조사후 3시간 군까지 밀도가 증가하였으며, 상피세포가 재생되면서 대조군과 유사해 질수록 미융모의 밀도는 다소 낮아진다. 다섯째, 점막 세포는 1시간 군까지 경한 세포탈락이 있을 뿐 시간경과에 따른 유의성이 없었다. 여섯째, SEM은 방사선 조사시 소장 점막의 3차원적 구조를 관찰하는데 민감한 도구이다. 일곱째, 미융모는 방사선 상해를 받은 군에서 더 발달되어있다. The authors studied acute radiation injury of small intestine by scanning electron microscope. Rabbit as an experimental model was irradiated 2,000rads/once. After that, a segment of terminal ileum was obtained and examined with time. Changes of villi are most severe on the 20 min experimental group and after that the severity was gradually regressed. With time, change of villi becomes mild and nearly normal on the 4day experimental group. Change of size and distribution of microvilli are relatively constnat with time except mild microvesicular changes to the 3hr group. Density of microvilli is usually higher on the irradiated group than control group. Focally cell loss is present but there is no remarkable changes with time on the individual cells. Following are conclusions of this experiment. First, radiation injury is most severe on the 20 min group. Second, shape of villi is close to normal from 1hr group, which suggests that this radiatio ninjury is reversible one. Third, flat villi or giant cell was not observed. Fourth, density of microvilli is increased to the 3hr group but regressed after that. Fifth, mucosal cells reveal relatively unremarkable change with time except mild cell loss to the 1hr group. Sixth, SEM is relatively sensitive method to see 3 dimensional structure of mucosa after radiation injury. Seventh, change of microvilli are more severe on the irradiated group.

裡里貴金屬·寶石 輸出團地의 加工技術의 改善 및 製品디자인 開發에 對한 硏究

吳隆京 圓光大學校 1982 論文集 Vol.16 No.2

A. Views and purpose of the study As one of enterprises for academic research and exploitation study in Korean Trades Scholarship Foundation, I was met with study of improvement in technology and invention in productions design in I Ri Jewelry Export Industrial Complex, receiving academic research funds of 1979. The prupose of this study is to act Korean Trades Scholalarship, contributing to development of local society, accelerating improvement and application of technology, specially contributing and development of I Ri Jewelry Export Industrial Complex, as donig this study with assistants and commission from Korean Trades Scholarship Foundation. B. Contents of the study (A) Range of the study present conditions and problems for technology processing precious metals. (B) Epitome of the study 1. Problems in process of jewelry casting and method of improvement to the problems. 2. Problems in metal face treatment. 3. Improvement of functional diagram in soldering and method of application in international creterion to solder. 4. Present conditions in method of Gold alloy and Silver alloy and comparison with international creterion. 5. Method of invention to productions design. 6. Various problems through on-the-spot survey.

두경부 편평상피암에서의 MIB-1, p53, bcl-2와 apoptosis의 발현 및 상호 연관성

손용준,한지용,윤형기,장수원,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Squamous cell carcinoma of the head and neck represents approximately 2.0∼4.0% of all malignant tumors, with an etiology closely associated with smoking, drinking and various genetic alterations. The purpose of this study was to investigate the correlation of MIB-1, p53, bcl-2 and apoptosis according to degree of differentitation of squamous cell carcinoma. Methods and Material : forty-eight cases of squamous cell carcinoma of the head and neck were selected for this study. MIB-1, p53, bc1-2 expression rate were studied by iromunogistochemical staining and the number of apoptosis observed by TUNEL method. Results : The expressioins of MIB-1, p53, bcl-2 and apoptosis were found in 56.3%, 50.0%, 14.6%, 85.4%, of 48 cases of squamous cell carcinoma, respectively. The expression of MIB-1, p53 and apoptosis were more higher in poorly differentiated cases than well differentiated cases. MIB-1 expression was related with p53 mutation (p<0.05) and apoptosis rate(p<0.05). Conclusion : From these results. the expressions of MIB-1, bcl-2 and apoptosis were related with degree of differentiation of squamous cell carcinoma and correlation among these parameters.

太陰人 男學生의 血液變化에 對한 硏究

金敬堯,韓宗鉉,洪淳用 圓光大學校 韓醫學硏究所 1992 원광한의학 Vol.2 No.1

Comparative blood analysis was carried out to investigate the change of blood constituent in Taeeumin(太陰人) group and control group. RBC, WBC, hemoglobin, hematocrit, total protein, triglyceride, phospholipid, total cholesterol, LDL-cholesterol, HDL-cholesterol, BUN, creatinine, ACTH, cortisol and prostaglandin E and F_2α were measured. And the following results were obtained: 1. In the change of blood cell, the value of hematocrit showed significant difference, while that of RBC, WBC and himoglobin didn't. 2. In the change of protein, the value of total protein showed significant difference, but that of albumin didn't. 3. In the change of lipid and cholesterol in serum, the value of triglyceride, phospholipid, total cholesterol and LDL-cholesterol indicated significant difference, while that of HDL- cholesterol indicated significant difference, while that of HDL-cholesterol didn't. 4. The value of BUN in serum represented significant difference, but that of creatinine didn't. 5. The value of cortisol in plasma showed significant difference, but that of ACTH didn't. 6. The value of prostaglandin E and F_2α in plasma showed significant difference. These results suggest that investigation on hormones and blood constituents enables objectification in differentiation of four types of physical constitution.

구강 편평세포암종의 Matrix Metalloproteinase(MMP)-1, 2, 3, 9와 Tissue Inhibitors of MMP(TIMP)-1, 2의 발현 양상의 비교 및 상호 연관성

노영서,한지용,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : nvasiveness of squamous cell carcinoma in oral cavity is associated with poor prognosis, and MMPs have an important role in degradation of extracellular matrix and bawement membrane of vessil walls. Increased expressions of MMPs is related to infiltrative growth and lymph node metastases in human cancers. In normal tissue, there is balanced secretions of MMPs and TIMPs, but impaired balance of MMPs and TIMPs was noted in cancer tissue. The aim of this study is to evaluate the expression rates of MMP-1, 2, 3 and TIMP 1 and 2 in squamous cell carcinoma of the oral cavity, and relationship between MMPs and TIMPs expression and histologic grade and invasion pattern. Methods and Material : Pathologic review of fifty cases of squamous cell carcinoma during a period of Mar. 1994 to Feb. 2000 based on histologic grade and invasion pattim and immunohistochemical stains for MMP-1, 2, 3 and 9 and TIMP-1, 2 were done. Statistical analysis between MMPs and TIMP s expression rate and pathologic parameters and relationship between MMPs and TIMPs were performed. Results : Expression rates of MMP-1, 2, 3 and 9were 32.0%, 38.0%, 22.0% and 20.0%, respectively. MMP-2 expression rate was higher in well differentiated carcinomas and in cases with less-infiltrative pattern. Other MMPs show slightly higher expression rate in well differentiated carcinomas, but there were no statistical significances. There were no significant differences of MMP-1, 2, 3 and 9 expression rates according to invasion pattern. Expression rates of TIMP-1 and TIM-2 were 22.2% and 48.0%, respectively, and there were no significant differences according to histologic grade and invasion pattern. Significant correlations of MMP-1, 2, 3 and 9 expressions except between MMP-2 and MMP-3 were noted, but there were no significant correlations between MMPs and TIMPs expression. Conclusion : Conclusions: In well differentiated and less infiltrative squamous cell carcinomas of the oral cavity, expression rates of MMPs, especially MMP-2, were high. And there were positive correlation between MMPs, but no correlation between MMPs and TIMPs. These findings suggest that MMPs may have a role in tumor developent of a subset of squamous cell carcinoma with less aggressive behavior and MMPs may involve in early stage of tumor progression.

재생 폴리에텔렌의 기계적 성질 : 충격 보강재 효과에 대하여

계형산,강경민,박종훈,정융권,이상환 목원대학교 자연과학연구소 2002 自然科學 硏究論文集 Vol.11 No.2

본 연구는 재활용이 가능한 폴리에틸렌 필름 등에 비산회(Fly Ash) 등의 충진재를 사용하여, 신재와 물성을 비교하여 재생 PE의 재활용 가능성 및 사용 가능한 충진재의 종류를 알아보는데 그 목적이 있다. 또한 본 연구에서는 치합형 동방향 회전 이축 압출기(Fully Intermeshing Co-rotating Twin Screw Extruder)를 이용해서 재생 Polyethylene에 비산회 및 다양한 충진재(filler)를 첨가하여 ASTM에 의거하여 충격시편을 만들었고, 각 충진재의 무게비에 따른 충진재 충격 시험을 하였으며 Capillary Rheometer를 이용하여 유변학적인 성질을 분석해 보았다.

구강에 발생한 편평상피암종의 MMP-2, MMP-9, TIMP-1, TIMP-2 발현과 EGFR 및 E-cadherin 발현의 상호연관성

조성재,권창석,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Matrix metalloproteinases(MMPs) are involved in the degradation of extracellular matrix, which is related to infiltrative growth and metastasis of tumor. Positive correlation between MMPs expression and clinico-pathologic parameters were reported, and MMPs expressions were regulated by tissue inhibitor of metalloproteinases(TIMPs) or cell adhesion molecules such as E-cadherin and epidermal growth factor receptor EGFR). The aim of this study is to evaluate the relationship between MMP-2, MMP-9 expressions and clinico-pathologic factors such as clinical stage. recurrence. histologic grade and invasion pattern, and between MMP-2 and MMP-9 Expressions and TIMP-1, TIMP-2, and E-cadherin expressions. Methods and Material : The materials were 55 cases of squamous cell carcinoma of the oral cavity, and immunohisdtochemical studies for MMP-2, MMP-9, TIMP-1, TIMP-2, E-cadherin and EGFR were performed. Clinico-pathologic factors such as clinical stage, recurrence, histologic grade and invasion pattern were recorded through microscopic examination and clinical record review. Statistical analyses between MMP-2, MMP-9 expressions and clinico-pathologic factors, TIMP-1, TIMP-2 expressions and EGFR and E-cadherin expressions were done. Results : 1. MMP-2 and MMP-9 expressions were noted in 30(54.5%) and 22(40.0%) of 55 cases, respectively. TIMP-1 and TIMP-2 expressions were observed in 21(38.2%) and 33(60.0%), and E-cadherin and EGFR expressions were found in 35(63.6%) and 26(47.3%) of 55 cases, respectively. 2. MMP-2 expression rate was slightly higher in cases without recurrence, and TIMP-2 expression rate was slightly higher in cases showing more infiltrative growth pattern. However, there were no significant differences of MMP-2, MMP-9, TIMP-1, and TIMP-2 expression rates according to other clinico-pathologic parameters were noted. 3. The expression rate of EGFR was higher in cases with well differentiation (p=0.0047), but no positive relationship between the expression rate of E-cadherin and histologic grade was found. There were no significant differences of the expression rates of E-cadherin and EGFR according to other clinico-pathologic parameters. 4. Cases with positive reaction for MMP-9 showed an increasing tendency of negative reaction for TIMP-1. However, signigicant relationships between MMP-2, MMP-9 expressions and TIMP-1, TIMP-2 expressions were not noted. 5. The expression rate of MMP-2 was higher in cases with positive reaction for Ecadherin and EGFR with no statistical significance. The expression rate of MMP-9 was significantly higher in cases with positive reaction for E-cadherin(p=0.0221), but there was no significant relationship between MMP-9 and EGFR expression. Conclusion : These results suggest that MMP-2, MMP-9, TIMP-1 and TIMP-2 expressions are involved in the development of oral squamos cell carcinomas, but MMP-2, MMP-9, TIMP-1, and TIMP-2 expressions might not seem to be a sueful prognostic factors because there were no significant relationship between clinicopathologic parameters, EGFR expression showed positive correlation with low histologic grace, so EGFR expression could be regarded as a good prognostic factor. In the progression of squamous cell carcinoma of oral cavity, MMP-9 and E-cadherin might be involved with positive correlation, but there were no significant correlations between MMP-2, MMP-9 and TIMP-1, TIMP-2 and EGFR expressions rate. Further study for other factors such as cytokines or growth factors except TIMP-1, TIMP-2 and EGFR will be needed.

Filtration Mechanism of nC₆₀ Transport in Porous Media Caused by not Straining but Attachment

( Yoong Kyung Jung ),( Yeong Min Kim ),( Jae Young Kim ) 한국폐기물자원순환학회 2011 ISWA Vol.2011 No.0

Tarantella in the Fourth movement of Franz Schubert’s Piano Sonata in C minor, D. 958

Yoong-Kyung Ahn The Pianissimo 음악예술학회 2012 음악예술연구 Vol.2 No.2

슈베르트는 그의 마지막 세 개의 피아노 소나타 D. 958-960를 1828년 9월, 약 두 달여의 생애를 남겨두고 완성하였다. 그 가운데에서 다단조 (C minor) 소나타, D. 958는 장조의 조성을 지닌 나머지 두 소나타에 달리 소용돌이치는 듯한 격한 감정과 음산한 분위기가 두드러진다. 특히, 이 소나타의 마지막 악장은 타란텔라 (tarantella) 음악에 전형적으로 보여지는, 쉼없는 셋잇단음표 음형의 지속적 사용이 돋보인다. 남부 이탈리아 지방의 민속춤에서 유래한 타란텔라는 끊임없는 셋잇단음표 음형을 특징적으로 나타내며, 체력적 에너지를 모두 고갈시킬 때까지 광적으로 추는 춤이라고 전해진다. 타란텔라 민속춤이 거미 (tarantula)의 독을 치유한다고 믿어진 전설의 진위 여부와 상관없이, 19세기 작곡가들은 그 춤이 지닌 이국적 활기나 생명력에 매료되었다. 그리하여 슈베르트를 비롯하여 쇼팽, 베버, 리스트와 같은 작곡가들은 타란텔라 리듬을 이용한 연주회용 작품을 내놓기도 하였다. 슈베르트의 소나타, D. 958의 마지막 악장 역시 이러한 타란텔라 음악의 예로써, 일종의 론도 형식으로 쓰여져 있다. 반복적으로 나타나는 후렴구 (Refrain)는 피할 수 없는 운명을 표현하는 듯하며, 대조적인 성격의 삽입구 (Episode)가 긴장감을 한층 높인다. 이 형식적 구성에 더하여, 악장 내 다채로운 화성적 변화는 고조된 극적인 전개를 부각시킨다. 예를 들어, 슈베르트의 전형적인 음악적 특징 중 하나로 지적되는 장조와 단조 사이의 잦은 전조는 희망의 환영과 가차없는 현실 사이의 모순을 드러내는 듯하다. 또, B major의 대조적인 중간 부분을 지배하는 달콤한 선율선은 슈베르트의 유명한 가곡, “마왕 (Erlkönig)”의 중간 부분에서 소년을 죽음으로 유혹하는 마왕의 감미로운 선율을 연상하게 한다. 슈베르트는 피아노 소나타 D. 958의 마지막 악장에서 악장 전체에 타란텔라의 전형적인 특징들을 도입함으로써 숨막히는 긴박감을 표현하고 있다. 후렴구를 지배하는 이 리듬적 추진력과 함께 삽입구에 드러나는 유혹적인 선율은 그의 임박한 죽음을 예견하는 듯하다. This paper examines how Schubert incorporates tarantella in the last movement of his piano sonata in C minor, D. 958. Written in 1828 a few months before he died, the movement exhibits darkness and restless vitality, as if foreshadowing the impending death. Its persistent triplets in a rapid tempo, typical of tarantella, express urgency and highly-charged tension throughout the movement. Along with the driving rhythmic energy, the melodic and harmonic language of the movement evokes the image of death. This paper first discusses the origin of tarantella, with regard to its notorious legend. Derived from a folk dance in Taranto, a city of Southern Italy, tarantella is believed to heal the fatal bite of tarantula, although its authenticity is doubtful. The mysterious “tarantella” legend, however, attracted the 19th-century composers. The anxiety and vigor associated with the folkdance often inspired the composers to conceive a so-called ‘tarantella rhythm.’ Schubert’s C-minor Sonata, D. 958, is one of the examples to use tarantella in the finale to bring the entire work to a brilliant close. The paper then analyzes form, melody, rhythm, and harmony of the movement. Cast in a variant of a rondo form, the movement consists of refrains and episodes. Episodes contrasting in character increases the tension accumulated by recurring refrains. Particularly, the middle episode in B major recalls the Erlking in Schubert’s song, “Erlkönig.” As in the song, its thrilling melody in the remote key elevates the intensity into an extremely tense atmosphere. Besides, the frequent modulations between major and minor keys are evident. In result, the kaleidoscopic harmony highlights the driving force by juxtaposing breathtaking sweetness and inevitable fear of death.