메치실린 내성 포도상구균에 대한 SK-302의 시험관내 및 생체내 효과

박윤선,김수기,신운섭,최선주,정종순,이종수 關東大學校醫科大學醫科學硏究所 1998 關東醫大學術誌 Vol.2 No.1

SK-302 is the active compound purified from culture filtrate of a streptomyces species (streptomyces sp. 302). The in vitro and in vivo antibacterial activities of SK-302 were evaluated against 6 clinically isolated MRSA strains and compared with the activities of vancomycin. SK-302 had node in virto antistaphylocoal activity stronger than that of vancomycin exhibiting MIC's, while vancomycin showed very weak 4 to 8fold lower than that of vancomycin. In SK-302 showed killing activity at the MIC, so SK-302 was superior to vancomycin. Furthermore, in acute systemic infections in mice, the ED_50s of SK-302 against infections by high-resistance MRSA were 30 to 40-feold less than that of vancomycin, therefore SK-302 showed potent in vive therapeutic efficacy against MRSA infection, These results suggest that SK-302 has excellent in virto in and in vivo activities against high-resistance MRSA.

소아 야뇨증의 치료

송윤섭,박영호 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Nocturnal enuresis is a symptom rather than a disease. Lots of theories have been proposed to explain why children with nocturnal enuresis fail to recognize or respond to their full or contracting bladder during sleep. Although these theories, which include behavioral, genetic, developmental, neurologic, psychological, urodynamic, and organic causes, are diverse and may be able to explain selected cases, there is no single explanation for this symptom, and in each individual multiple factors may be operative. Clearly, the vast majority of children with nocturnal enuresis do not suffer from psychiatric, neurologic, or urologic disturbances, and investigation and treatment along these lines is both inappropriate and unrewarding. A number of treatment modalities have been used for nocturnal enuresis, but their effectiveness, even in controlled studies, has been difficult to assess because of the high spontaneous annual remission rate (15%) and the extremely high placebo improvement effect, which can exceed 65%. Proven, reproducible, and effective therapy has evolved along two lines: drug therapy and behavioral modification. The physician who cares for patients with enuresis must develop an orderly, practical, and effective approach to evaluating and treating the children who present with nocturnal enuresis.

3D-CT를 이용한 골격성 III급 개방교합자의 악교정 수술 전, 후 설골 및 상기도의 변화

이윤섭,백형선,이기준,유형석 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.2

본 연구는 골격성 III급 개방교합자의 악교정 수술 전, 후 설골과 상기도의 변화를 3D-CT를 이용하여 관찰하고 이를 정상교합자와 비교하기 위하여 시행하였다. 전치부 개방교합을 동반한 골격성 III급 부정교합으로 진단되어 악교정 수술을 받은 환자 중 12명을 선정 후, 3D-CT를 통해 설골과 상기도의 3차원 입체영상을 분석하여, 악교정 수술 전, 후의 설골의 위치 변화 및 상기도의 부피 변화를 살펴보고, 이의 결과를 정상교합자 10명과 비교하였다. 설골은 골격성 III급 개방교합군에서 악교정 수술 전에 정상교합군의 설골에 비해 전방에 위치하고 있었고 악교정 수술 후 설골은 후, 상방으로 변화하였으나 유의성은 관찰되지 않았다. Hyoid plane과 mandibular plane이 이루는 각은 악교정 수술 전의 골격성 III급 개방교합군이 정상교합군보다 큰 값을 나타내었고, 악교정 수술 후에는 그 차이가 더욱 증가하였다. 골격성 III급 개방교합군의 상기도 부피는 정상교합자보다 작으며 이는, 악교정수술 후에 더욱 감소하였다. 3차원 영상 분석 결과, 골격성 III급 개방교합자의 상기도는 정상교합자에 비해 좁으며, 하악골 후퇴술 이후 더욱 감소하기 때문에 이는 수술적 개선의 안정성에 영향을 줄 수 있음을 알 수 있었다. Objective: The purpose of this study was to investigate the structural changes of the hyoid bone and upper airway after orthognathic surgery for skeletal class III anterior open bite patients, and make comparisons with normal occlusion. Methods: Pre- and post-operative computed tomography (CT) examinations were performed on 12 skeletal class III anterior open bite patients who were treated with mandibular setback osteotomy. Using the V-works 4.0 program, 3-dimensional images of the total skull, mandible, hyoid bone, and upper airway were evaluated. Results: In the Class III openbite group, the hyoid bone were all positioned anteriorly, compared to the Normal group (p < 0.05). The angle between the hyoid plane and mandibular plane in the Class III openbite group before surgery was greater than in the Normal group (p < 0.05), and the difference increased after surgery (p < 0.01). In the Class III openbite group, the volume of the upper airway decreased after surgery (p < 0.001) and the volume of the upper airway was smaller than the Normal group before and after surgery (p < 0.001). Conclusions: The narrow upper airway space in skeletal Class III openbite patients decreased after mandibular setback osteotomy. This may affect the post-surgical stability.

메치실린 내성 황색포도알균의 신속한 검출을 위한 multiplex PCR

박윤선,신운섭 關東大學校 醫科大學 醫科學硏究所 2004 關東醫大學術誌 Vol.8 No.2

Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of nosocomial infections as well as community acquired infections. The rapid identification of MRSA is very important for the appropriate antibiotic therapy, and MRSA must be differentiated from coagulase-negative staphylococci, which may be members of normal microbial flora. However, the conventional phenotypic methods for MRSA detection often lack sensitivity, because of the hetrogeneous expression of methicillin resistance and the dependence of resistance expression on the test conditions. In this study, we developed a multiplex PCR (MPCR) assay for rapid and accurate detection of MRSA. Two pairs of primers were used, which yielded two PCR products. One PCR product is a 310-bp mecA-based PCR fragment specific for methicllin resistance, and the other is a 108-bp S. aureus-specific DNA fragment. The multiplex PCR developed in this study showed excellent specificity and sensitivity for MRSA detection. When three methods for DNA extraction from MRSA were compared, lysostaphin methods and bead beating methods were more efficient than the heating methods. Therefore, the MPCR using two pairs of primers is thought to be a very sensitive and specific tool for rapid detection of MRSA, especially when the DNA of MRSA is extracted by bead beating method.

항이디오타입 종양 백신

신운섭,박윤선 관동대학교 의과대학 의과학연구소 2005 關東醫大學術誌 Vol.9 No.1

Anti-idiotypic antibodies (Ab2) that bind to the antigen-combining sites(paratopes) of anti-tumor antibodies (Ab1) may functionally and structurally mimic the tumor antigen defined by the Ab1. Thus, the anti-idiotypic antibodies resembling the original tumor antigen are called internal images of the tumor antigen and can be used as surrogate antigens for active specific immunotherapy. The advantages of Ab2 vaccines over conventional antigen vaccines are their high specificity, safety, ease of production, and potential to break immune tolerance to tumor antigen. The basic mechanism for immune tolerance breakage by Ab2 vaccines is a kind of cross-reaction between tumor antigen and Ab2 at T cell level. Anti-idiotypic antibodies have been shown to induce antigen-specific humoral and cellular immune reponses in experimental animals and cancer patients. In this paper, we will describe the idiotypic network, the advantages of Ab2 vaccines over conventional antigen vaccines, the mechanism of action of Ab2 vaccines, and the preclinical and clinical trials of active specific immunotherapy with Ab2 vaccines.

Trinitrophenyl Cellulose의 조제

맹정섭,남윤규,박승희,최우영 충남대학교 농업과학연구소 1995 농업과학연구 Vol.22 No.2

Two types of modified celluloses which contain trinitrophenyl groups as chromophore were synthesized from carboxymethyl cellulose Whatman CM 70 and CM 32. Diaminoethyl groups were added to the CM 70 and CM 32 to make DAE-CM celluloses and then the DAE-CM groups were substituted by 2,4,6-trinitrophenyl groups to produce TNP-celluloses. Average particle size of the TNP-cellulose from CM 32 was 44.6 ±9.6 μm in diameter and 127.9±22.5 μm in length, which was much smaller than those from CM 70, however its TNP-moiety per gram determined by using the molar extinction coefficient 1.33×10^4 of ε-TNP-lysine at 345 nm, was 0.68 millimoles, which was 5.6-fold greater than those from CM 70. The absorption spectrum of TNP-oligosaccharides which were the soluble products of TNP-celluloses by a cellulase preparation Onozuka R-10, showed a maximal peak at 344 nm. Increases in the absorbance during hydrolysis were linear with the enzyme concentration, and the differences of slope values between two types of TNP-celluloses that the more semsitive assay could be achieved by using those from CM 32 as substrate at the low range of the enzyme concentration.

韓國産 小麥의 製빵 敵性에 關한 硏究

林潤熙,盧完燮 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.2

The primary objective of this study was tested the baking properties of Korean wheat. In test results, the wheat which was harvested from all over the Korea, not suitable for making bread and cake products. However, some of possibilities to make bread were found by using vital gluten as well as additives. Out of Korean wheat classes, the Tapdong wheat showed good properties for bread making, and it is recommended to develope it as bread making wheat by segregating from other classes in seeding and harvesting.

인도메타신이 개의 유치 치근 흡수에 미치는 영향

신강섭,강윤구,이기수 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

이 연구는 흡수중인 유치를 대상으로 인도메타신이 치근흡수에 미치는 영향을 조사하고, 치근흡수에 관련된 주위 조직의 변화를 관찰하기 위하여시행되었다. 인도메타신은파골세표의 수를 감소시키고 골흡수를 감소시키며, 골형성도 감소시키는 것으로 보고되어 왔으나 형태와 기능이 유사하다는 파치세포에 미치는 영향에 관한 연구는 희소하다. 생후 12-13주된 잡견 6마리를 통상적으로 복용량인 인도메타신 2㎎/㎏/day를 14일간 투여한 군과 과량의 8㎎/㎏/day를 14일간 투여한 군과 대조군으로 구분하였으며 흡수중인 하악 절치를 연구대상으로 하였다. 연구대상 치아는 5㎛ 두께의 절편을 만들고, H & E 중염색. Masson의 trichrome 염색을 시행하고 광학현미경으로 검경하였으며,파치세포의 수와 핵의 수를 비교하였다. 관찰 결과 유치 치근 흡수 조직은 골개조 소견과 함께 염증 소견과 유사한 소견을 보였다. 흡수중인 유치의 치수는 치근흡수부위에 가까운 조상아세포층은 변성의 소견을 보이나 멀리 떨어진 치수는 정상인 소견을 보였으며 인도메타신이 투여된 실험군에서는 파치세포의 수적 감소와 핵의 수적 감소를 미약하게 나타냈다. 그러나 인도메타신이 흡수중인 치아의 치수에 미치는 영향은 관찰되지 않았다. 이상의 결과에 의하면 인도메타신은 파치세포의 수적 감소를 미약하게 일으키며, 장기간의 인도메타신 투여는 유치 치근흡수의 지연을 초래할 가능성이 있음을 시사한다. The study was aimed to invesigate the effects of indomethacin on physiologic root resorption and to examine the dental pulp and tissue changes around the resotbing teeth. 13-14 week old six mongrel dogs were divided into 3 groups: two experimental groups administered indomethacin 2㎎/㎏/day and 8㎎/㎏/day orally two times a day for 14 days respectively, and control group administered a placebo. The deciduous incisors showing root resorption were selected, fixed for 24hrs in 10% formalin solution, demineralized in 10%EDTA solution, invested in paraffin and sectioned in 5㎛ thick sections. The preparations were staine with H & E staining and Masson's trichrome staining and examined unger the light microscope. Observation revealed that deciduous root resorbing tissue resembles inflammatory tissue and accmpanies bone remodelling. The dental pulp was normal except the area near root resorption, well organized columnar odontoblasts layer under the predentin, and the odontoblasts near root resorption were cuboidal or flat cells in the disrupted layer under the predentin. Indomethacin administered group showed a partial decrease in the number of odontoclasts and nucleus. But there was no sign of pulp change by indomethacin. These results suggest that indomethacin inhibits recruitment of odontoclasts partially and that of osteoclasts more, and so when it is administered for long periods, deciduous root resorption can be delayed and eruption of the successor can be delayed for a short period.

TNP-cellulose의 섬유소 분해효소 활성도 측정을 위한 기질로서의 특성

맹정섭,남윤규,최우영 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.2

Characteristics of TNP-cellulose which prepared from carboxymethyl cellulose powder, CM32, as substrate for cellulase activity assay were investigated. Enzymatic hydrolysis of TNP-cellulose occured on the cellulose moiety but not on amide bonds, following Michaelis-Menten kinetics. Tree cellulase preparations from Trichoderma viride, Aspergillus niger. and Cellulomonas sp. were tested for their pH and temperature dependences and compared with the method determining the increase in reducing power. The enzyme activity was found to have the same temperature range in both methods, however the pH range was broadened in the case of using TNP-cellulose as substrate. The colorimetric method for cellulase assay using TNP-cellulose as substrate was compared with the other methods: one based on determination of the increase in reducing power; and the other based on determining the decrease in viscosity of Na-CM-cellulose solution. The activities measured by the colorimetric method showed a linear correlation with the enzyme concentration of certain range in all three enzymes tested, and the activity values were proportional to those obtained from the other methods. Depending on the enzyme. however, the activity values from this method were not always in proportion to those from the viscometric method, suggesting that this method was not specific for determination of the endo-type cellulase.

임신과 동반된 난소섬유난포막종 1례

류은석,김윤숙,전섭,김종수,최승도,선우재근,배동한,양승화 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Fibrothecoma are mesenchymal tumors deriving from the ovarian stroma and consisting of theca-like elements and fibrous tissues. The reported incidence is between 0.4 and 8.0% of all ovarian tumors and this side variability can be attributed to the difficulty in differentiation fibrothecoma from ovarian fibroma or hyperplastic stroma nodules. We experienced a rare case of ovarian fibrothecoma in woman at 38 weeks gestation with previous repeat cesarean section diagnosed after tertiary cesarean delivery and bilateral tubal ligation with a brief review of literature.