Lactobacillus (LA-1) and butyrate inhibits osteoarthrits through controling autophagy and inflammatory cell death in chondrocyte

Keun hyung Cho,Hyun-Sik Na,JooYeon Jhun,Jiyoung Kim,Seung Yoon Lee,Jeong soo Lee,In Gyu Um,Seok Jung Kim,Mi-La Cho 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Osteoarthritis (OA) is a disease that reduces quality of life due to pain caused by persistent joint destruction. In addition, as a representative chronic disease, it causes inflammation and affects immunity, and it is one of the diseases that is difficult to cure, so treatment and improvement methods are urgently needed. In a previous study, we published that LA-1 improves osteoarthritis and has cartilage protection by controlling inflammation. However, it was not known how LA-1 improves osteoarthritis in the body. So in this study, it was confirmed that the administration of LA-1 to the MIA-induced OA rat model reduces the pain threshold, protects cartilage, and regulates inflammation markers in the articular synovium. Additionally, collecting and analyzing the feces of the disease model, it affected the gastrointestinal system and improved the environment of the microbiome. Interestingly, by providing LA-1, it was confirmed that the diversity and abundance of microbiome in the intestine were changed, and that the bacteria that produced SCFAs increased. In addition, daily supply of butyrate, one of the SCFAs produced by certain bacteria, triggers autophagy activation and tends to decrease necroptosis. This suggests that systemic immunity as well as OA is regulated according to changes in the intestinal microbial community, and that activation of autophagy can indirectly reduce abnormal cell death. In addition, assuming that osteoarthritis is a chronic degenerative disease, cell analysis was performed using splenocyte and blood assuming that the immune system is deteriorated. As a result, both splenocytes and PBMCs confirmed that regulatory T cells increased and Th17 cells decreased. In summary, providing LA-1 leads to increased production of SCFAs by altering the microbes in the intestine. Accordingly, it is possible to suppress the progression of OA and control pain due to OA, and improve an abnormal joint environment by controlling autophagy and necroptosis.

Diagnostic approach for intra-abdominal and retroperitoneal mass

Yoon La Choi 대한종양외과학회 2020 대한임상종양학회 학술대회지 Vol.2020 No.7

Case Report : Wire-Guided Endoscopic Snare Retrieval of Proximally Migrated Pancreatic Stents after Endoscopic Papillectomy for Ampullary Adenoma

( La Young Yoon ),( Jong Ho Moon ),( Hyun Jong Choi ),( Seul Ki Min ),( Sang Woo Cha ),( Young Koog Cheon ),( Young Deok Cho ),( Moon Sung Lee ),( Jae Seon Kim ) 대한간학회 2011 Gut and Liver Vol.5 No.4

With the increasing use of pancreatic duct (PD) stents after endoscopic papillectomy (EP), complications such as proximal migration of the stent have become increasingly prevalent. A PD stent that migrates within a nondilated PD may be difficult to remove endoscopically. We performed endoscopic retrieval of proximally migrated PD stents after EP in 5 patients. Endoscopic retrieval was performed immediately after EP in one patient, the next day in 3 patients, and 2 weeks later in one patient. Wire-guided endoscopic retrieval was attempted in 4 patients, and the migrated stents were removed successfully in these 4 patients. No signifi cant procedure-related complications occurred, other than mild pancreatitis in a single patient. In one patient, endoscopic retrieval performed immediately after EP failed when using the conventional method, and the migrated stent was removed using a minisnare without a guidewire the next day; this patient developed severe pancreatitis. Wire-guided endoscopic snare retrieval seems to be a safe and effective method for removing proximally migrated PD stents after EP. (Gut Liver 2011;5:532-535)

Time Domain Analysis of Pulse Rate Variability according to the Sampling Rate Change

Yoon La Yang,Hangsik Shin 한국지능시스템학회 2016 한국지능시스템학회 학술발표 논문집 Vol.26 No.1

Hypothermia Inhibits Endothelium-Independent Vascular Contractility via Rho-kinase Inhibition

( Yoon Hee Chung ),( Keon Woong Oh ),( Sung Tae Kim ),( Eon Sub Park ),( Hyun Dong Je ),( Hyuk-jun Yoon ),( Uy Dong Sohn ),( Ji Hoon Jeong ),( Hyen-oh La ) 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.2

The present study was undertaken to investigate the influence of hypothermia on endothelium-independent vascular smooth muscle contractility and to determine the mechanism underlying the relaxation. Denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Hypothermia significantly inhibited fluoride-, thromboxane A_2-, phenylephrine-, and phorbol ester-induced vascular contractions regardless of endothelial nitric oxide synthesis, suggesting that another pathway had a direct effect on vascular smooth muscle. Hypothermia significantly inhibited the fluorideinduced increase in pMYPT1 level and phorbol ester-induced increase in pERK1/2 level, suggesting inhibition of Rho-kinase and MEK activity and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxing effect of moderate hypothermia on agonist-induced vascular contraction regardless of endothelial function involves inhibition of Rho-kinase and MEK activities.

단일공법 복강경하 난소낭종절제술과 고식적 방법과의 비교

이윤정 ( Yoon Jung Lee ),김명주 ( Myung Joo Kim ),성석주 ( Seok Ju Seong ),김인현 ( In Hyun Kim ),김미라 ( Mi La Kim ),송태종 ( Taejong Song ),윤보성 ( Bo Sung Yoon ),정용욱 ( Yong Wook Jung ),주원덕 ( Won Duk Joo ),전혜선 ( Hye S 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.5

목적 양성 난소낭종절제술에서 단일공과 고식적 복강경수술의 결과를 비교하여 단일공수술의 유용성과 안전성을 알아보고자 하였다. 연구방법2010년 1월부터 2011년 7월까지 단일공 복강경하 양성 난소낭종절제술을 받은 28명을 진단명에 따라 3군(자궁내막종, 성숙기형종, 그외)으로 분류하여 각 1:2비율로 고식적 복강경하 난소낭종절제술 받은 56명을 선정하여, 후향적인 의무기록 분석을 시행하였다. 결과환자의 나이, 체질량지수, 기존의 복강내 수술 여부나 낭종의 양측성 및 낭종의 평균 크기는 두 군 간 유의한 차이가 없었다. 유착박리술여부, 동시에 시행한 수술 여부, 실혈량, 수술시간, 수술 전후 혈색소 수치변화, 추가 진통제 사용 여부, 재원기간의 수술 결과에 있어서 양군 간 유의한 차이가 없었다. 그러나 낭종 종류별 분석결과, 자궁내막증에서 실혈량의 중위값은 단일공군 30 mL (범위, 30-200 mL), 고식적 복강경군 100 mL (범위, 30-450 mL)로 유의한 차이를 보였고( P = 0.017) 수술시간 또한 고식적 복강경 군의 중위값 60분(범위, 40-85분)으로 단일공군의 40분(범위, 30-100분)보다 유의하게 길었다( P = 0.004). 성숙기형종에서는 수술시간, 실혈량은 두 군 간 유의한 차이가 없었으나 수술 후 혈색소 수치감소 중위값은 단일공군에서 2.3 g/dL (범위, 2.0-3.1 g/dL)로 고식적 복강경수술을 받은 군인 1.4 g/dL 범위, 0.1-1.8 g/dL)보다 의미 있게 컸다( P = 0.002). 결론 배꼽 절개를 통한 단일공 복강경수술은 양성 난소낭종절제술에 있어 안전하고 유용하며, 기존의 고식적 복강경하 수술의 대안이 될 수 있다. Objective This study was conducted to compare the surgical outcomes between single-port access (SPA) and conventional laparoscopic ovarian cystectomy. Methods This retrospective, matched case-control study was performed in 84 cases, who underwent SPA laparoscopic ovarian cystectomy (cases: 28) by a single surgeon and conventional laparoscopic ovarian cystectomy (control: 56) by another surgeon who had similar surgical skill at our hospital between January 2010 and July 2011. Results All procedures were performed successfully in both groups without conversion to explo-laparotomy. There was no significant difference in demographic characteristics including size of ovarian cysts, estimated blood loss (EBL), operation time, hemoglobin change, postoperative hospital stay and number of patients needed extra anti-infl ammatory drugs between two groups. Analysis according to the type of cysts, median EBL (30 mL vs. 100 mL, P = 0.017) was higher in conventional group and operation time (40 minutes vs. 60 minutes, P = 0.004) was also longer in conventional group in case of endometrioma. The EBL, median operation time and postoperative hospital days were similar in both group, but median hemoglobin change was less in conventional group (2.3 g/dL vs. 1.4 g/dL, P = 0.002) in mature cystic teratoma patients. There were no signifi cant difference of surgical outcomes in others group. Conclusion SPA laparoscopic ovarian cystectomy using conventional laparoscopic instruments is feasible, effective and reliable technique, which can substitute conventional laparoscopy.

생체신호 기반 IoT 디퓨저 개발을 위한 기초연구

양윤라(Yoon La Yang),신항식(Hangsik Shin) 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.7

생체신호기반 조명, 향기 요법 제공을 위한 IoT 디퓨져 개발

양윤라(Yoon La Yang),강성탁(Seongtak Kang),신항식(Hangsik Shin),김동준(Dong Jun Kim) 대한전기학회 2015 정보 및 제어 심포지엄 논문집 Vol.2015 No.10

광용적맥파 진폭변이도 시간영역 분석을 통한 술후 통증 평가 가능성 고찰

양윤라(Yoon La Yang),신항식(Hangsik Shin) 한국지능시스템학회 2018 한국지능시스템학회논문지 Vol.28 No.3

이 연구는 광용적맥파(PPG, photoplethysmogram) 진폭관련 파라미터 분석을 통해 술후 통증 평가에 있어 광용적맥파 활용가능성을 고찰한다. 이를 위해 수술 전, 후의 광용적맥파를 획득 하였고 광용적맥파의 수축기 및 이완기 특징점으로부터 진폭과 관련된 단일 파라미터인 기저선-수축기 진폭(ACAbaseline), 이완기-수축기 진폭(ACAdia), 인접 박동간 수축기 값 차이(PPGAVsys), 인접 박동간 이완기 값 차이(PPGAVdia)와 이를 정규화한 파라미터인 PPGAVsys/ACAbaseline, PPGAVsys/ACAdia, PPGAVdia/ACAbaseline, PPGAVdia/ACAdia를 도출하였다. 도출된 파라미터로부터 연속된 이산 데이터 시간영역분석에 주로 사용되는 평균(AVG) 표준편차(SD), 차분표준편차(SDSD), 차분실효값(RMSSD)을 계산하였고 수술 전과 후에 유의미한 변화를 보이는 파라미터를 확인하였다. Paired t-test 결과 단일 파라미터의 경우 ACAbaseline의 AVG와 SD, ACAdia의 AVG과 RMSSD가 수술 전과후 유의미한 차이(p<0.01)를 보였고 정규화된 파라미터의 경우 모든 분석에서 유의미한 차이(p<0.001)를 보였다. 피험자간 편차를 의미하는 변동계수는 PPGAVsys/ACAbaseline의 AVG에서 가장 작은 값을 보였다. In this research, we investigate the feasibility of photoplethysmogram(PPG) in assessing the postoperative pain through amplitude analysis of PPG. PPG was recorded in pre- and post-operation. Parameters which reflects amplitude, AC amplitude from baseline to systolic peak(ACAbaseline), AC amplitude from diastolic peak to systolic peak(ACAdia); parameters which reflects the amplitude change, systolic PPG amplitude variation(PPGAVsys), diastolic PPG amplitude variation(PPGAVdia); normalized parameters; PPGAVsys/ACAbaseline, PPGAVsys/ACAdia, PPGAVdia/ACAbaseline and PPGAVdia/ACAdia were extracted with systolic and diastolic feature of PPG waveform. Then, we calculated average(AVG), standard deviation(SD), standard deviation of successive difference(SDSD), and root mean square of standard deviation(RMSSD), which are commonly used for time domain analysis of consecutive discrete data from the derived parameters, and observed parameters that showed significant changes pre- and post-operation. Consequently, significant difference(p<0.01) was found in AVG, SD of ACAbaseline and AVG, RMSSD of ACAdia, and every normalized parameters(p<0.01). Coefficient of variation, which reflects the individual difference, has the lowest value in AVG of PPGAVsys/ACAbaseline.

혈액종양 ; 비편평세포, 비소세포폐암 환자에서 erlotinib, gefitinib 및 pemetrexed의 효과

윤라영 ( La Young Yoon ),양미연 ( Mi Yean Yang ),윤진아 ( Jin A Yun ),김현정 ( Hyun Jung Kim ),김한조 ( Han Jo Kim ),김경하 ( Kyung Ha Kim ),김세형 ( Se Hyung Kim ),이상철 ( Sang Cheol Lee ),김찬규 ( Chan Kyu Kim ),이남수 ( Nam Su 대한내과학회 2010 대한내과학회지 Vol.79 No.4

목적: 본 연구는 비소세포폐암의 2차 이상의 항암 화학 요법에서 EGFR TKIs와 pemetrexed의 임상적인 특징에 따른 치료 성적을 비교함으로써 구제치료의 선택에 도움을 주고자 하였다. 방법: 2002년 2월부터 2009년 8월까지 부천 순천향대학교 병원 혈액종양내과에서 비소세포폐암을 진단받은 환자들 중 편평상피암을 제외한 선암과 대세포암 환자들을 대상으로 하였으며 이들 중 gefitinib이나 erlotinib 및 pemetrexed로 치료받은 환자를 의무기록을 통해 후향적으로 조사하였다. 결과: 총 61명이 연구에 참여하였고 그 중 erlotinib군이 18명, gefitinib군은 18명, pemetrexed군은 25명이었으며 중앙관찰기간은 37개월(7-97개월)이었다. 세 군간에 반응율과 무진행 생존기간에는 의미있는 차이가 없었으며 EGFR TKIs 를 투여받은 환자들 중에서는 여성에서 보다 좋은 반응율은 보였고, erlotinib 을 투여받은 환자들 중 피부발진이 있었던 환자들과 Pemetrexed를 투여 받은 환자들 중 선암으로 진단된 경우에서 무진행 생존기간이 의미있게 길었다. 결론: EGFR TKIs 와 Pemetrexed 는 모두 비편평세포암, 비소세포암 환자에서 2차 이상의 치료로 사용하였을 때 비교적 효과적이었으며 부작용도 적은 것으로 나타났다. 향후 EGKR TKIs 및 pemetrexed군 치료 효과 및 치료 효과에 영향을 미치는 다양한 임상적 예후인자에 대한 보다 많은 대규모 전향적 비교 연구가 필요할 것이다. Background/Aims: This study compared the clinical benefits of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) with pemetrexed to identify the clinical parameters that correlated with response. Methods: A retrospective chart review examined patients who were 1) treated with EGFR TKI or pemetrexed, 2) diagnosed with advanced non-squamous non-small-cell lung cancer, and 3) previously treated with platinum-based chemotherapy in Soonchunhyang Bucheon Hospital. Results: Sixty-one patients (18 erlotinib, 18 gefitinib, 25 pemetrexed) were investigated from February 2002 to August 2009. The median follow-up period was 37 months (7~97 months). Overall, their median age was 63 years, 41 patients were non-smokers, 57 patients had adenocarcinoma, and 55 patients were at stage IV. Twenty-one patients received the study drugs as second-line chemotherapy, and others as third-line or more. No significant differences in the overall response rate (erlotinib 33.3% vs. gefitinib 38.9% vs. pemetrexed 20.0%) and progression-free survival (erlotinib 1.9 months vs. gefitinib 3.0 months vs. pemetrexed 2.9 months) were found among the three groups. Female gender was related to a good response to EGFR TKIs (p=0.047). Skin rash in the erlotinib group (p=0.037) and adenocarcinoma in the pemetrexed group (p=0.02) were related to improved progression-free survival. Few side effects were reported. Conclusions: Both EGFR TKIs and pemetrexed therapy for non-squamous non-small-cell lung cancer were efficient and tolerable after the failure of first-line platinum-based chemotherapy. Further prospective studies are needed to validate the predictive role of the suggested clinical parameters in this study. (Korean J Med 79:394-403, 2010)