Determination of Acyl-lysophosphatidic Acid in Plasma by Tandem Mass Spectrometry

Yoon, Hye-Ran 덕성여자대학교 약학연구소 2005 藥學論文誌 Vol.16 No.1

Analysis of acyl lysophosphatidic acids(LPAs) has clinical importance as a potential biomarker for ovarian and other gynecological cancers or obesity in the aspect of prevention. Here we report simple sample preparation and analytical method developed with high sensitivity and specificity for the early detection of gynecological cancers to improve the overall outcome of this disease. We established a novel quantification method of acyl-LPAs in plasma by electrospray negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring mode without conventional TLC step. Protein-bound lipids, acyl-LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as internal standard under acidic condition. Following back extraction with chloroform and water, centrifuged lower phase was evaporated and reconstituted in methanol and then analyzed. Using ESI-MS-MS with negative ionization MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:3 LPA, respectively. Q2 ions selected for MRM was m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAa were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01 - 25 (mol/L. The method detection limit was 0.3 pmol/ml with correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas with normal and gynecological cancer patient, this new method differentiated two different groups by way of total LPA level.

Do Subtypes of Focus Really Exist in Spanish?

Hye-Yoon Chung 한국언어학회 2016 언어 Vol.41 No.1

Chung, Hye-Yoon. 2016. Do Subtypes of Focus Really Exist in Spanish? Korean Journal of Linguistics, 41-2, 97-118. This article deals with the term focus in Spanish, in particular, two subtypes of focus, called Contrastive Focus and Non-contrastive Focus (cf. Gutiérrez-Bravo, 2005). According to Zubizarreta (1998), in Spanish, the two types of focus most noticeably differ syntactically: While Non-contrastive Focus should appear at the utterance-final position, Contrastive Focus may appear in-situ. Inspired by a handful of studies (e.g. Cabrera-Abreu & García-Lecumberri, 2003; Kim & Avelino, 2003; Toledo, 1989) and motivated by empirical data gathered for the pilot study conducted prior to the current study, I set out to reinvestigate Zubizarreta’s (1998) claim on the distinction between the focus subtypes. The findings of the current study show that the pragmatically defined notion of focus (Lambrecht, 1994) is not further divided into two types in Castilian Spanish at least syntactically. Across the focus scopes, there were little differences in Contrastive Focus and in Non-contrastive focus. (Korea University)

소방공무원의 정신건강 문제 예방을 위한 해피아트테라피 프로그램 적용 사례연구

윤혜선 ( Hye-sun Yoon ),윤현서 ( Hyunseo Yoon ) 한국무용과학회 2022 한국무용과학회지 Vol.39 No.1

이 연구의 목적은 소방공무원 대상 해피아트테라피(Happy Arts Therapy: 이하 H.A.T.)프로그램의 적용 사례를 통해 소방공무원의 정신건강 문제 예방을 위한 통합예술치유 프로그램의 효과적인 적용 방안을 탐색하고자 하였다. 이에 이 연구는 2018년 8월부터 2020년 2월까지 서울소방재난본부 소속 소방학교와 소방서·119안전센터에서 787명의 소방공무원을 대상으로 총 88회 시행된 H.A.T.프로그램을 연구하기 위해 다양한 정보원들을 활용하여 상세하고 심층적으로 보고하는 사례연구 방법을 통해 ‘H.A.T. 프로그램의 구성과정’과 ‘프로그램 참여경험의 의미’ 및 ‘평가내용’을 분석하였다. 첫째, 소방공무원 대상 H.A.T. 프로그램의 내용 구성은 윤혜선(2009)이 개발한 H.A.T. 프로그램의 기본 구조를 기반으로 소방공무원의 특성을 반영하여 ‘긍정적 대인관계’, ‘스트레스 해소’, ‘자기이해’, ‘심리적 회복탄력성 증진’을 프로그램 내용 구성의 주안점으로 도출하였다. 또한 소방공무원 대상 H.A.T. 프로그램의 체계적인 운영 시스템 구축을 위하여 운영과정에 참여한 구성원들은 상호 협력 관계를 통해 역할분담과 환경구축 과정에서 지속적으로 협의하여 운영 체계를 구성하였다. 둘째, 소방공무원의 H.A.T. 프로그램 참여 경험의 의미를 범주화하여 분석한 결과, ‘동료 간의 소통 및 유대감 경험’, ‘심리적·신체적 회복의 경험’, ‘자기 이해의 경험’으로 분류 할 수 있었다. 셋째, 이 연구에서 H.A.T. 프로그램의 평가는 참여 기관 자체 평가자료와 언론보도, 참여자 평가와 운영 구성원의 평가 자료를 중심으로 평가사항을 분석하였는데, 이를 통해 프로그램에 대한 소방기관의 긍정적인 평가를 확인하였고, 차후 프로그램 운영과정의 개선방안을 탐색하였다. 이 연구의 결과는 향후 소방공무원의 정신건강을 위한 예술치유 프로그램 및 심리지원 프로그램의 효과적인 적용방안을 모색하는 연구의 기초자료로 활용되길 기대한다. The purpose of this study is to explore effective application plans for integrated art healing program to prevent mental health problems in fire-fighting officers through the application cases of the Happy Arts Therapy(Hereinafter referred to as H.A.T.) program for fire-fighting officers in this study. Accordingly, in this study ‘the composition process of the H.A.T. program’, ‘meaning of program participation experience’, and ‘evaluation contents’ were conducted in order to study the H.A.T. program implemented a total of 88 times through a case study method that uses various sources of information. The subjects of the study are 787 fire-fighting officers at a fire school, fire station, and 119 Safety Center belonging to the Seoul Fire and Disaster Headquarter from Aug. 2018 to Feb. 2020. The results are as follows. First, the content composition of the H.A.T. program for fire-fighting officers was mainly drawn as ‘positive interpersonal relationship’, ‘relieve stress’, ‘self-understanding’, and ‘promote psychological resilience’ reflecting the characteristics of fire-fighting officers based on the basic structure of the H.A.T. program developed by Hye-Sun Yoon(2009). In addition, members who participated in the operating process continuously consulted in the process of division of roles and environmental construction through mutual cooperation in order to establish a systematic operation system for the H.A.T. program for fire-fighting officers. Second, it could be classified into ‘communication and bonding experience between colleagues’, ‘psychological and physical recovery experience’, and ‘self-understanding experience’ as a result of categorizing and analyzing the meaning of participation experience in the H.A.T. program of fire-fighting officers. Third, the evaluation of the H.A.T. program was analyzed focusing on proprietary evaluation data of participating agencies, media reports, participant evaluations, and evaluation data from the operation members in this study. A positive evaluation of the program was confirmed by the fire institution and a plan to improve the program operation process in the future was explored. It is expected that the results of this study can be used as basic data for research seeking effective application of art healing programs and psychological support programs for the mental health of fire-fighting officers in the future.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

호산구성 간농양 54예의 임상상

강혜련,권혁수,송우정,이상민,김태범,박혜경,장윤석,김윤근,조상헌,민경업,김유영 대한알레르기학회 2004 천식 및 알레르기 Vol.24 No.2

Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats

Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*

치매환자 조호자를 위한 교육지지프로그램의 효과성에 관한 비교연구

송 흡(Heup Song),강재명(Jae Myeong Kang),김병규(Steven B. Kim),조성진(Seong Jin Cho),윤선경(Seon kyung Yun),윤혜진(Hye jin Yoon),연병길(Byeong Kil Yeon) 대한노인정신의학회 2017 노인정신의학 Vol.21 No.1

Objective:We studied the effect of Hyu-Sig-Gong-Gan and Hye-Ah-Rim on several evaluations and compared the two programs. We then investigated factors associated with the difference between pre- and post-ratings. Methods:Thirty-six patients were randomized to Hyu-Sig-Gong-Gan (n=23) or Hye-Ah-Rim (n=13). We tested for the effect of each program on several evaluations and compared the two programs through Zarit Burden Interview (ZBI), Center for Epidemiologic Studies-Depression Scale, Dementia Attitude Scale (DAS)-intelligence, DAS-comfort and DAS-total. We then investigated whether the difference between pre- and post-rating depends on other factors. Results:We observed significant positive effect of Hyu-Sig-Gong-Gan on DAS-comfort (t=1.843, p=0.039) and significant positive effect of Hye-Ah-Rim on ZBI (t=-2.327, p=0.019), DAS-comfort (t=2.241, p=0.022), and EuroQol-5 Dimensions-Visual Analogue Scale (t=2.537, p=0.013). For comparing the two programs, we found a lack of evidence for the difference in any of the five commonly observed evaluations. In the secondary analysis, we observed a strong trend that the two programs were particularly effective for caregivers with negative pre-ratings. Conclusion:The two programs showed positive effects on the various evaluations, but some evaluations could not reach statistical significance. The programs appear to be particularly helpful for those who are in need before the program participation.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

입원전담전문의 진료지원시스템의 설계 및 도입과 병원정보시스템과의 통합 방안

정윤빈,신형식,우가희,정혜경,정진경,고미영,김진수,김소원,이강영 건강보험심사평가원 2021 HIRA RESEARCH Vol.1 No.2

분지형 협착 혈관 모형을 이용한 도플러 파형에 관한 연구

윤혜경,박재형,이동혁,원형진,김성현,서준범,연경모 대한초음파의학회 1998 ULTRASONOGRAPHY Vol.17 No.2