韓國人에서 VNTR D1S80 遺傳座位의 遺傳的 多樣性 및 集團의 均質性 檢定

明賢君,黃迪駿,洪鎔杓,金致弘 大韓法醫學會 1994 대한법의학회지 Vol.18 No.1

Human genomic DNA prepared from whole blood sample was analyzed to estimate allelic frequencies and genetic diversity at a hypervariable variable number of tandem repeats(VNTR) D1S80 locus in 127 Korean. Target DNA fragments were amplified by polymerase chain reaction(PCR) and separated by polyacrylamide gel electrophoresis. Then, the following results were obtained. 1. A total of 23 alleles were identified at this locus, which is the biggest number of alleles observed at this locus so far(cf. 16 from U. S. Caucasians and 15 from Finnish). 2. Sixty-two genotypes, included 3 homozygotes and 59 heterozygotes, out of a total of 276 probable genotypes were also observed, which might be resulted from the small sample size compared to the number of alleles observed in this study. 3. General pattern of allelic distributions was concordant with those observed in previous studies from Finnish and U. S. Causccasians where two alleles, M18 and M23, were observed most frequently and the rest of them were observed rare(<10%) with the exception of M30 allele(14.6%) in Korean. The number of M30 allele is suspected to be increased in Korean at or after the divergence of Korean from the hypothetical ancestor probably caused by random genetic drift such as founder effect of bottleneck effect. 4. Higher level of heterozygosity was obtained in Korean(H=0.866 and H=0.881) than in Finnish(H=0.77 and H=0.79) and U. S. Cauccasians(H=0.808 and H=0.797). 5. Population homogeneity was tested to corroborate the obtained population genetic parameters calculated based on the assumption of Hardy-Weinberg equilibrium, which could be done by Chi-square test between the observed number of alleles and the expected number of alleles, because of the small sample size analyzed in this study which resulted in high proportion of undetected genotypes (78.5%) and several genotypes significantly deviated from hardy-Weinberg expectation in a sample population. The result suggests that samples were drawn from geneticically homogeneous population. 6. This study suggests that Korean is more diversified than Finnish and U. S. Cauccasians on the basis of the number of alleles and the level of heterozygosity, although Korean is known to be entirely composed of single race. Though we need more informations from other Asian countries, this result suggests frequent gene flow from neighbor countries, which is also expected based on the old Korean history of frequent invasions from Japan and China. 7. Based on the Hardy-Weinberg expectation, 216 out of 253 genotypes at D1S80 locus can be shared by less than 5 individuals among 1,000 unrelated individuals by chance. Our results suggest that the analysis of VNTR D1S80 locus can provide powerful, but imperfect by itself, DNA markers for forensic study, which can be corroborated by analyzing additional VNTR loci.

플라즈마 침탄과 CrN 코팅된 Ti-6Al-4V 합금의 미세구조 및 기계적 특성

박용권,위명용 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.12

In order to improve the low hardness and low wear resistance of Ti-6Al-4V alloy, plasma carburization treatment and CrN film coating was carried out. Effects of the plasma carburization and CrN coating were analyzed and compared with the non-treated alloy by microstructural observation, structural characterization and mechanical property test. The plasma carburization treated alloy formed a carburized layer of about 150 mm in depth from the surface, where a fine and hard particles of TiC and V₄C₃ were evenly homogeneously dispersed through the layer. CrN thin film thickness was about 7.5 mm and grew with (111) orientation. Hardness value of about Hv 402 in the matrix was improved as Hv 1,600, 1,390 by plasma carburization, CrN thin film, respectively. Roughness test is an important method to evaluate the surface treatment effect on the wear resistance. The optimum surface roughness of about 0.063 mm could be obtained by CrN film coated, while, about 0.321 mm and 0.211 ㎛ in the matrix and plasma carburization specimens, respectively.

기대손실함수를 이용한 다특성치 파라미터 설계

조용욱,박명규 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

When designing the parameter on the multiple quality characteristics, there has been a study for optimization of problems, but there has been no former study on the possible conflicting phenomena in consideration of the correlations among the characteristics. To solve the issue on the optimal design for multiple quality characteristics, this study modelled the expected loss function with cross-product terms among the characteristics and derived range of the coefficients of the terms. The model will be used to determine the global optimal design parameters where there exists the conflict among the characteristics, which shows difference in optimal in optimal design parameters for the individual characteristics.

밀폐계 측정장치를 이용한 물-노말프로판올 계의 인화점

하동명,최용찬,이성진 한국산업안전학회 2002 한국안전학회지 Vol.17 No.4

The Flash Point is one of the most important combustible properties used to determine the potential for fire and explosion hazards of chemical materials. An accurate knowledge of the flash point is important in developing appropriated preventive and control measures in industrial fire protection. The lower flash points for the Water + n-Propanol systems were measured by using Pensky-Martens closed cup tester. The experimental data were compared with the values calculated by the laws of Raoult and van Laar equation. The calculated values based on the van Laar equation were found to be better than those based on the Raoult's law.

분광 엘립소메트리의 응용을 위한 0차 Retardation Plate의 Alignment

이용달,박명규,김세덕,방경윤,안일신 한양대학교 이학기술연구소 2000 이학기술연구지 Vol.2 No.

A zero-order retarder was fabricated using two pieces of magnesium fluoride plates. In order to achieve desired zero-order retardation for specific wavelength, both the thickness of plates and the alignment of two fast axes were very important. We found that the alignment should be checked using broad band detection system for spectroscopic application, We devised alignment method by which the two fast axes lie perpendicularly to each other. The periodic oscillation feature in retardation angle versus wavelength disappeared with good alignment. We also developed calibration method to deduce the retardation angle over a wider spectral range using rotating compensator ellipsometer. 두 조각의 마그네슘 플로라이드 판을 이용한 0차 retarder를 제작하였다. 특정한 파장에 대하여 원하는 0차 retardation을 얻기 위해서는 판의 두께와 alignment가 매우 중요하였다. 분광학적인 응용을 위해서는 넓은 파장 영역을 측정할 수 있는 detection system의 사용이 필요하였다. 이를 이용하여 두 판의 fast axes가 서로 수직으로 놓이게 할 수 있는 alignment 방법을 개발하였다. 파장에 대한 retardation 각도에서의 주기적인 진동의 모양은 alignment를 잘하게 되면 사라지게 된다. 또한 rotating compensator ellipsometer를 사용 할 때 넓은 분광영역에 걸친 retardation angle을 측정해 낼 수 있는 calibration 방법을 개발하였다.

유산균 투여가 건강한 성인의 분변미생물 및 부패산물 생성에 미치는 영향

신명수,김용재,배형석,백영진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

유산균의 장내균총과 부패산물 생성에 미치는 영향을 알아보기 위하여, 건강한 성인 남녀 6명의 지원자에게 Lactobacillus acidophilus 와Bifidobacterium longum으로 제조한 유산균분말(각각 1.5×10 exp (9) cells)을 하루에 2회씩 2주동안 섭취하도록 하였다. 그리고 지원자 분변으로부터 균총수 변화와 부패산물인 암모니아, indole, skatole, p-cresole량을 측정하였다. 유산균의 투여 기간중에 분변내 bifidobacteria의 수가 8.78±0.39(log cfu/g feces±S.D)에서 9.27±0.29로 증가하였고(p<0.05) 투여 중지후에는 약간 감소하였으며, lactobacilli의 수는 6.15±0.80에서 6.76±0.48로 증가하였다(p<0.05). Enterococcus 균수는 투여 기간중에 6.66±0.80에서 7.72±0.40으로 증가하였다가 투여 중지후에는 감소하는 경향을 보였다. 그리고 혐기성균인 Bacteroides는 투여중에 8.45±0.34에서 9.15±0.21으로 증가하였으나(p<0.01), 유해균주로 알려진 Closridium(lecithinase negative)과 Staphylococcus 균수는 현저히 감소하였다. 그러나, 유산균 투여 실험기간인 6주 동안 분변내 암모니아, indole, skatole, p-cresole의 유의적인 농도변화는 없었다. To investigate the effects of lactic acid bacteria administration on fecal microflora and putrefactive metabolites in human being, Lactobacillus acidophilus and Bifidobacterium longum powder (1.5×10 exp (9) cells, respectively) was administrated to six healthy volunteers (average 28 years old) twice a day for 2 weeks. During the administration of lactic acid bacteria, the numbers of bifidobacteria, lactobacilli, and enterococci in feces were increased significantly, whereas those of Staphylococcus and lecithinase-negative Clostridium were decreased considerably. In addition, a number of anaerobic Bacteroides were increased. However, the contents of fecal ammonia and putrefactive metabolites (indole, skatole, p-cresole) were not changed during the administration.

플라즈마 침탄 및 CrN 코팅된 Ti-6Al-4V 합금의 기계적특성

박용권,위명용 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.2

In order to improve the low hardness and low wear resistance of Ti-6Al-4V alloy, plasma carburization treatment and CrN film coating was carried out. Effects of the plasma carburization and CrN coating were analyzed and compared with the non-treated alloy by microstructural observation, structure characterization and mechanical property test. The plasma carburization treated alloy formed a carburized layer of about 150㎛ in depth from the surface, where a fine and hard particles of TiC and V_4C_3 were homogeneously dispersed through the layer. CrN thin film thickness was about 7.5㎛ and grew with (111) orientation. Hardness value of about Hv 402 in the matrix was improved as Hv 1600, 1390 by plasma carburization, CrN thin film, respectively. Roughness test is an important method to evaluate the surface treatment effect on the wear resistance. The optimum surface roughness of about 0.063 ㎛ could be obtained by CrN film coated, while, about 0.321 ㎛ and 0.211 ㎛ in the matrix and plasma carburization specimens, respectively.

가중치를 고려한 다특성치 파리미터 설계에 관한 연구

조용욱,박명규 明知大學校 産業技術硏究所 1999 産業技術硏究所論文集 Vol.18 No.-

Taguchi's parameter design is to determine optimal settings of design parameters of a product or a process such that the characteristics of a product exhibit small variabilities around their target values. the critical problem in dealing with mutiple characteristics is how to compromise the conflicts among the selected levels of the design parameters for each individual characteristic. In this paper, Methodology using SN ratio optimized by univariate technique is proposed and a parameter design procedure to achive the optimal compromise among several different response variables is developed. one new case studies are solved by the proposed method and the results are compared with ones by the sum of SN ratios, the expected weighted loss, the desirability function, and EXTOPSIS model.

光照射에 의한 콩나물의 質的 變化

金明姬,元惠心,李正和,林容順,張美花 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

光의 照射에 의한 콩나물의 質的變化를 調査할 目的으로 光度 및 光質別 處理를 하여 成長狀態, Vitamin C, chlorphnyll, cellulose등의 含量과 質感에 對한 官能檢査를 行하였으며 chlorophyll과 vitamin C의 關係를 chloramphenicol 處理에 의하여 調査해 보았다. 그 結果 白色光의 경우 50 lux와 100 lux 處理로 vitamin C의 含量 增加와 cellulose등의 生成이 促進되었으며 質感이 暗所보다 良好하였다. 그러나 200 lux 하에서는 매우 不良하였다. blue光은 他光보다 cellulose와 vitamin C生成에 效果的 이었다. chlorophyll과 vitamin C의 含量과는 直接的인 關係가 없고 다만 光照射와의 關聯性이 있는 것으로 推定되었다. This study was carried out to evaluate the quality of green soybean sprouts grown under light. The soybean was germinated under light through blue, red and white polyethylene films at 25℃ for 10 hours a day, and light intensity was controlled 50 to 200 lux. The contents of Vitamin C. cellulose and chlorophyll were determined and checked up texture by sensory evaluation of the soybean sprouts soup. In addition, the relationship of chlorophyll and Vitamin C was measured by treatment of chloramphenicol which has inhibitory effect of chloroplast, chlorophyll accumulating site. Vitamin C and cellulose contents were all increased by irradiation of white light at 50 and 100 lux and also texture of the sprouts was better than it of the sprouts grown under darkness while, it was very tough under 200 lux. Blue light was more effective for biosynthesis of Vitamin C and cellulose than white and reg light. Biosynthesis of chlorophyll was not relative to Vitamin C content directly. but the relationship may be concerned with light irradiation.

도시하천의 이용행태와 물리적 요소에 대한 만족도

김용수,이동명,박찬용 경북대학교 농업과학기술연구소 2006 慶北大農學誌 Vol.24 No.-

The purpose of this study is to plan, design and reflect to the future naturalization of the urban rivers by using the literatures, field survey results and aerial photography of Milyang River as the case regarding the effect of physical factors of urban rivers on landscape, defining those factors as Sammon fine forest, outdoor stage, Youngnamlu, RC stadium, stroll roads and bicycle roads, artificial island, outdoor swimming pool and sculpture park, and by suggesting the specific characteristics of the landscape according to those factors. The questionnaire survey was made to understand the users' satisfaction and attributes. The statistical analysis was applied, the frequency analysis and percentage calculation were used to find out the citizenship and perception toward the physical factors, and paired-sample T test to compare the average.