http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ko, Young‐,Ho,Kim, Je‐,Hyung,Jin, Li‐,Hua,Ko, Suk‐,Min,Kwon, Bong‐,Joon,Kim, Joosung,Kim, Taek,Cho, Yong‐,Hoon WILEY‐VCH Verlag 2011 ADVANCED MATERIALS Vol.23 No.45
<P>Electrically driven hybrid light‐emitting diodes (LEDs) consisting of quantum dots, wires, and wells based on the nanometer‐sized pyramid GaN structure are reported by Taek Kim, Yong‐Hoon Cho, and co‐workers on page 5364. The LEDs exhibit mixed emissions from InGaN quantum dots, wires, and wells formed at the tops, edges, and sidewalls of the pyramids, respectively. The hybrid LEDs containing low‐dimensional quantum structures provide a broad‐band, highly efficient visible lighting source. </P>
Molecular Analysis of the Copper-Responsive CopRSCD of a Pathogenic Pseudomonas fluorescens Strain
Yong-hua Hu,Hua-lei Wang,Min Zhang,Li Sun 한국미생물학회 2009 The journal of microbiology Vol.47 No.3
CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.
Yong Xiao,Liang Sun,Qi Wang,Qiang Zhang,Shao-Hua Gu,Adel Khashaveh,Ze-Wen Liu,Yong-Jun Zhang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
The alfalfa plant bug Adelphocoris lineolatus (Goeze) (Hemiptera: Miridae), a serious pest of several agricultural crops, is extremely attracted by flowering plants to perform season host switching. Generally, chemoreception of A. lineolatus plays a crucial role in detecting food sources, locating mates and oviposition sites. In addition, odorant carrier proteins are believed to participate in the initial chemical communication and perception in insects. In this work, we identified 31 putative odorant carrier protein genes from the antennal transcriptomes of A. lineolatus, including 17 new odorant binding proteins (OBPs), 10 new chemosensory proteins (CSPs) and four Niemann-Pick type C2 proteins (NPC2s). Phylogenetic analysis demonstrated that the OBP and CSP genes from four mirid bug species (A. lineolatus, Adelphocoris suturalis, Apolygus lucorum and Lygus lineolaris) had a relatively close evolutionary relationship. Regular patterns and key conserved motifs of OBPs and CSPs in Hemiptera insects are also identified by using Multiple EM for Motif Elicitation (MEME) tool. Tissue expression profiles analysis showed that nine of the 17 OBPs, 10 CSPs and two of the four NPC2s were primarily or uniquely expressed in the adult antennae suggesting their putative roles in chemoreception. Our study provides the first insight into the roles of odorant carrier proteins in chemoreception of A. lineolatus, which will help to facilitate biological functions of odorant carrier proteins and develop novel insect behavioral regulation strategy
PREPARATION OF CINOBUFAGIN-LOADED BOVINE SERUM ALBUMIN NANOPARTICLES FOR HEPATOCARCINOMA THERAPY
YONG-HUA SU,JIAN-GUO ZHANG,JIE SHEN,FENG-QIAN LI,HUA SU,CHANG-QUAN LING 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2009 NANO Vol.4 No.1
Cinobufagin-loaded bovine serum albumin nanoparticles were prepared for treating hepatocellular carcinoma. In this report, cinobufagin-bovine serum albumin-nanoparticles (Cino-BSA-NP) were prepared by an aqueous desolvation process. The physicochemical properties, toxicity, and cancer-related applications of Cino-BSA-NP were investigated. Cino-BSA-NP had a uniform spherical morphology with a particle size in the range of 50–240 nm and an average size of 86.3 nm. The zeta potential of the nanoparticles was -49 mV. The overall embedding ratio was 79.5% and the drug loading was 24.1%. Cino-BSA-NP gave cinobufagin release of up to 53.5% within 3 h, followed by slower controlled release. Cino-BSA-NP inhibited growth of hepatocarcinoma cells in vitro to a similar extent as free cinobufagin, but with a much higher median lethal dose (LD50). Hepatic histomorphological changes indicated that hepatic damage was much less severe with Cino-BSA-NP than with free cinobufagin (2.19 mg/kg). The survival time of nude mice with orthotopic transplantation tumors treated with Cino-BSA-NP was prolonged significantly. The results confirm that Cino-BSA-NP renders cinobufagin completely dispersible in aqueous media, meeting the key requirements for intravenous injection, and show controlled release, thus significantly improving cinobufagin's antitumor activity while reducing its side effects.
Yong Luo,Ling Lan,Yong-Guang Jiang,Jia-Hui Zhao,Ming-Chuan Li,Neng-Bao Wei,Yun-Hua Lin 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2
Although cancer stem cells (CSCs) play a crucial role in seeding the initiation of tumor progression, they do not always possess the same potent ability as tumor metastasis. Thus, precisely how migrating CSCs occur, still remains unclear. In the present study, we first comparatively analyzed a series of prostate CSCs, which exhibited a dynamically increasing and disseminating ability in nude mice. We observed that the transcriptional activity of HIF-1 and -catenin became gradually elevated in these stem cells and their epithelial-mesenchymal transition (EMT) characteristic altered from an epithelial type to a mesenchymal type. Next, we further used cancer-associated fibroblasts (CAFs), which were cultured from surgically re-sected tissues of prostate cancer (PCa) to stimulate prostate CSCs. Similar results were reconfirmed and showed that the protein levels of both HIF-1 and -catenin were markedly improved. In addition, the EMT phenotype displayed a homogenous mesenchymal type, accompanied with increased aggressive potency in vitro. Most importantly, the aforementioned promoting effect of CAFs on prostate CSCs was completely repressed after “silencing” the activity of -catenin by transfection of stem cells with ShRNA. Taken together, our observations suggest that prostate migrating CSCs, with a mesenchymal phenotype, could be triggered by CAFs in a HIF-1alpha/beta-catenin-depen-dent signaling pathway.
Yong Hua Zhang,Yong Cai Li,Zheng Hua Cai,Yang Chen 대한신경외과학회 2004 Journal of Korean neurosurgical society Vol.36 No.4
Objective : Using bilateral carotid artery occlusion, controlled hypotension and in vivo microdialysis, we evaluate the changes in extracellular concentrations of the excitatory amino acids(EAA) glutamate and aspartate in the striatum of ischemic Wistar-Kyoto rats(WKY) and spontaneously hypertensive rats (SHR), as well as the resulting changes of infarct volume and neurological deficit scores. Methods : During 30 minutes of ischemia, microdialysate concentrations of glutamate and aspartate were measured in the presence of infusion of artificial cerebrospinal fluid(aCSF) or the dihydrokinate (DHK, the GLT-1 selective nontransportable inhibitor)-containing aCSF respectively. And infarct volume of neurological deficit were measured for each groups using triphenyltetrazolium chloride(TTC) staining and neurological deficit function tests. Results : In control aCSF infusing WKY group, glutamate and aspartate concentrations increased 36-fold and 14- fold increase to baseline, respectively, and returned to baseline values on reperfusion; while in control aCSF infusing SHR group, glutamate and aspartate increased 51-fold and 25-fold increase respectively. DHK (10nM) significantly attenuated EAA increases in both experimental WKY group and SHR group compared with control (glutamate peak for WKY, 7.09±1.04 versus control, 18.48±1.87 pmol/ul; for SHR, 18.90±1.54 versus control, 40.64±2.38 pmol/ul). There is a significant improving with neurological deficit score in DHK application groups both in WKY and SHR, which was also convinced by comparison of infarct volume between experimental DHK infusing group and control. Conclusion : Our results suggest that the astrocytic glutamate transporter contributes significantly to EAA release in the early phase of ischemia and inhibition of excessive glutamate release by DHK may contribute to effective treatment in ischemia-induced brain damage.
The elastoplastic formulation of polygonal element method based on triangular finite meshes
Yong-chang Cai,He-hua Zhu,Sheng-yong Guo 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.30 No.1
A small strain and elastoplastic formulation of Polygonal Element Method (PEM) is developed for efficient analysis of elastoplastic solids. In this work, the polygonal elements are constructed based on traditional triangular finite meshes. The construction method of polygonal mesh can directly utilize the sophisticated triangularization algorithm and reduce the difficulty in generating polygonal elements. The Wachspress rational finite element basis function is used to construct the approximations of polygonal elements. The incremental variational form and a von Mises type model are used for non-linear elastoplastic analysis. Several small strain elastoplastic numerical examples are presented to verify the advantages and the accuracy of the numerical formulation.
Thermodynamics and Kinetics of Lysozyme Adsorption onto Two Kinds of Weak Cation Exchangers
Yong-Mei Cheng,Xiong-Hua Jin,Dong Gao,Hai-Feng Xia,Jing-Hua Chen 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5
The present work investigated the adsorption behaviors of lysozyme onto weak cation exchangers at different temperatures. The adsorption isotherm, adsorption thermodynamics and adsorption kinetics were studied. The results indicate that the adsorption of lysozyme onto acrylic acid copolymer based beads (Hydrolite D115) is spontaneous and exothermic, while that onto agarose based beads (CM Sepharose 6 Fast Flow) is also spontaneous, but endothermic. The pseudo second-order kinetic model fits well to the dynamic adsorption experimental data, and the kinetic results are also in concert with the adsorption thermodynamics.