Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

檀園 金弘道의 風俗畵 硏究

洪勇善 新羅大學校 1978 論文集 Vol.6 No.-

Kim, Hong-do (pen name:Dan-won) was the greatest genius in the art of the Yi Dynasty. In his genre paintings are expressed the common people's consciousness and the social life of his days. This is remarkable because genre paintings were generally considered vulgar by many and his courage as a genre painter defying the prevalent taste of the nobles was admirable. Thus the painting style of the later Yi Dynasty assumed a new aspect through his example. His paintings expressed the life and feelings of the underprivileged in all its everyday triviality. In contrast to one of his contemproaries, Hye-won, who is wellknown for his paintings of women, he showed marked interests on himan side in his realistic paintings He used humor and irony to paint the plight of the common people in their poverty. Dan-won's genre paintings had a great influence on his contemporeries and those who followed him. They exhibited the features of the epochal symbols in the later period of the Yi-Dynasy, and a new renaissance in the Korean art. It is deeply regretted that his style of painting declined after him during the 19th century without further expansion.

Comparison of methods for library construction and short read annotation of shellfish viral metagenomes

Hong‑Ying Wei,Sheng Huang,Jiang‑Yong Wang,Fang Gao,Jing‑Zhe Jiang 한국유전학회 2018 Genes & Genomics Vol.40 No.3

The emergence and widespread use of high-throughput sequencing technologies have promoted metagenomic studies on environmental or animal samples. Library construction for metagenome sequencing and annotation of the produced sequence reads are important steps in such studies and influence the quality of metagenomic data. In this study, we collected some marine mollusk samples, such as Crassostrea hongkongensis, Chlamys farreri, and Ruditapes philippinarum, from coastal areas in South China. These samples were divided into two batches to compare two library construction methods for shellfish viral metagenome. Our analysis showed that reverse-transcribing RNA into cDNA and then amplifying it simultaneously with DNA by whole genome amplification (WGA) yielded a larger amount of DNA compared to using only WGA or WTA (whole transcriptome amplification). Moreover, higher quality libraries were obtained by agarose gel extraction rather than with AMPure bead size selection. However, the latter can also provide good results if combined with the adjustment of the filter parameters. This, together with its simplicity, makes it a viable alternative. Finally, we compared three annotation tools (BLAST, DIAMOND, and Taxonomer) and two reference databases (NCBI’s NR and Uniprot’s Uniref). Considering the limitations of computing resources and data transfer speed, we propose the use of DIAMOND with Uniref for annotating metagenomic short reads as its running speed can guarantee a good annotation rate. This study may serve as a useful reference for selecting methods for Shellfish viral metagenome library construction and read annotation.

방사선 치료 후 Koebner 현상에 의해 발생한 것으로 사료되는 건선 1예

홍성신,이용주,김홍용 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.2

The Koebner phenomenon is the development of the isomorphic skin lesions in persons with certain skin diseases after an injury occurred on normal-appearing skin. This response can be developed in psoriasis, pityriasis rubra pilaris, verruca etc. Several types of injury can produce the Koebner phenomenon such as irritation, physical injury, surgical wound, and sunburn or radiation therapy. We report a case of psoriasis developed by Koebner phenomenon after radiation therapy and this is the first report in Korea. (Korean J Dermatol 2003;41(2) : 264∼266)

흰쥐에서 간담관 장애가 신장에 미치는 초미세 형태학적 연구

김용임,김일,이길부,최종범,기근홍,김용일,서재홍 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

It has been well-known that biliary cirrhosis with concomittant impairment of renal functions were occured after prolonged obstruction of biliary tract. In the present study, an attempt was made to investigate the mechanism of impaired renal function and the ultrastructural change of the kidney in rats, ligated the common bile duct for 21, 28 and 50 days in order to induce secondary biliary cirrhosis. The results obtained were as follows ; 1. Secondary biliary cirrhosis was produced evidently 28 days after ligation of common bile duct. Prominent proliferations of bile ducts and chronic inflammatory cell infiltrations were seen in pehportal area. 2. 28 and 50 days after ligation of common bile duct, show swelling and degeneration of mitochondria, microcystic formation and lysis of endoplasmic reticulum in the hepatocytes. Loss of microvilli in the bile ductules were noted. 3. Focal fusions, edema and bleb formations of foot processes were seen. Electron dense materials with variable size and global shape were found in foot process, 21, 28 and 50 days after ligation of common bile duct. 4. There were slightly thickening of renal glomerular basement membrane with hump in the subendothelial and widening of the mesangial matrix, 28 days after ligation of common bile duct. 5. There were moderately thickening of renal glomerular basement membrane and widening of the mesangial matrix with electron dense deposits, 50 days after ligation of common bile duct. From the above findings of electron microscopy, this study revealed electron dense deposits on renal glomerular mesangium of the rats as a results of experimental secondary biliary cirrhosis by ligation of common bile duct. Also severe hepatic injury due to swelling or lysis of rough endoplasmic reticulum and destruction of bile ductules resulted in deposited electron dense materials due to inhibition of excretion of immune complex through hepatobiliary tract, therefore this change indicated that immuncomplex could be deposited on renal glomerular mesangium.

음식물류폐기물 공공 자원화시설 운영에 관한 연구 (Ⅱ)

홍용표ㆍ김웅용ㆍ신현곤(Yong Pyo HongㆍUng Yong KimㆍHyun Gon Shin) 유기성자원학회 2016 유기물자원화 Vol.24 No.1

본 연구는 음식물류폐기물 공공 자원화 시설 운영의 문제점과 제도 개선방안을 위하여 실시하였다. 현재 운영중인 음식물류 폐기물의 가동율 현황과 자원화 시설의 확충 계획에 대한 분석을 통해 시설의 무분별한 신규건설로 인한 국가의 예산이 낭비되지 않도록 그 대안을 제시하였으며, 그 결과는 다음과 같다. 공공 자원화시설 신규 확충보다는 기존 민간 자원화시설을 활용하여 예산을 절약하고 음식물류 폐기물의 안정적인 처리는 물론 자원순환을 촉진시킬 수 있는 정책전환이 필요하다. 음식물류 폐기물을 친환경적인 방법으로 안정적으로 처리하기 위해서는 제도개선이 필요하며, 이를 위해 민간 자원화시설 가동률 저하를 줄일 수 있는 준공영제 도입이 필요하다. 또한, 공공 자원화시설의 검증된 기술과 경제성 확보 방안 마련이 필요하다. This study is conducted to find out the problems of the public food waste recycling facility operation and its improvement. In order to save the national budget by analyzing plans to expand including operation rate for the food waste recycling facility, the alternative ways are suggested. The result can be as follows: Policy switch is required not only to save budget and stable disposal of food waste but also to promote resource recycling rather than expanding public facilities. In order to change the policy for stable and eco-friendly disposal of food waste, it is necessary to adopt semipublic system that prevent the decline of the rate of operation of private resource recycling facility. Furthermore, it is required to prepare both qualified technologies of public resource recycling and the ways of profitability secure.

항정신병약물 사용 중인 정신분열병 환자에서 올란자판으로의 교체 방법에 관한 연구(II) : Comparison of Safety 안전성 비교

안용민,권용실,권준수,민성호,박두병,양문정,소형석,송종호,신윤식,우행원,유범희,이홍석,정한용,한창환,김용식 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.5

연구목적: 이 다기관 공동임상연구는 사용 중인 항정신병약물을 ’직접 교체 방법’또는 ’시작-감량 교체 방법’중 한 가지 방법으로 올란자핀으로 교체한 후, 안정성 측면에서 두 교체 방법 간의 비교와 교체후의 변화를 관찰하기 위한 것이다. 방법: 국내 13개 병원의 입원 및 외래에 내원한 환자들 중 ICD-10 지단기준으로 정신분열병에 해당되며, 임상적으로 항정신병약물 교체가 필요한 환자를 대상으로 하였다. 두 가지 교체 방법 중 한 가지를 무작위로 피험자에 적용하였으며, ’직접 교체 방법’에 배정된 경우에는 사용중인 항정신병약물을 일시에 중단하고 10㎎의 올란자핀을 바로 투여하였고, ’시작-감량 교체 방법’에 배정된 경우는 10㎎의 올란자핀 투여하고 2주에 걸쳐서 기존 약물을 감량하여 중단하였다. 올란자핀 사용기간은 총 6주이며, 용량은 5∼20㎎ 범위로 제한하였다. 한정성 평가를 위해서 체중, 생명징후, 자발적인 이상반응 복, 실험실 검사 그리고 Simpson-Angus Scale(SAS), Barnes akathisia rating scale(BARS), Abnormal involuntary movement scale(AIMS). Liverpool University neuroleptic side effect rating scale(LUNSERS)등을 이용하였다. 결과: 총 103명의 정신분열병 환자를 대상으로 하였다. 사용한 올란자핀의 용량, 벤조디아제핀의 병용률, 탈락률과 탈락 사유, 자발적인 이상반응 보고, 생명징후, 실험실 검사 그리고 대부분의 부작용 척도 상에서 임상적으로 의미 있는 차이를 두 교체 방법간에 발견하지 못하였다. 다만 AIMS의 감소는 ’직접 교체 방법’군에서 보다 적었고, 항콜린제의 병용률은 ’시작-감량 교체 방법’군에서 보다 많았다. 기저 상태에서 전체 피험자의 SAS와 BARS 점수는 각각 3.5점과 1.8점이었으며 70% 이상의 피험자가 고프로락틴 혈증을 보였다. 올란자핀으로 교체한 후, SAS, BARS, AIMS 점수의 유의한 감소가 있었으며 고프로락틴 혈증을 보인 피험자 분율도 약 30%이하로 감소하였다. 그러나 교체 방법과 상관없이 올란자핀 교체 후 유의한 체중 증가가 있었다. 결론: 이 연구를 통해 교체 방법에 관계없이 비교적 안전하고 용이하게 올란자핀으로 교체 할 수 있음을 알 수 있었다. 그리고 기존 항정신병약물을 올란자핀으로 교체함으로써 일부 부작용들을 줄일 수 있음을 간접적으로 관찰할 수 있었다. 하지만 이 연구는 여러 제한점과 문제점을 지니고 있기 때문에 보다 체계적인 연구를 통해 검정이 필요하리라 생각된다. Objectives: This multicenter clinical trial involving 13 hospital sites compared the safely of switching to olanzapine between ’direct switching method’ and ’start-tapering switching method’. Method: This study included both inpatients and outpatients who fulfilled the criteria for schizophrenia as defined in the ICD-10, and were in need to be appropriate for switching antipsychotics. Subjects were randomly assigned to one of the two switching methods. For ’direct switching method’group, previous antipsychotics were abruptly discontinued and 10㎎ of olanzapine was administered, and previous antipsychotics was gradually tapered for 2 weeks. Olanzapine was used for 6 weeks and the dose was adjusted within the range of 5-20㎎. The safety of switching to olanzapine was measured with vital sings including body weight, adverse events reported spontaneously, laboratory tests, and various scales such as Simpson-Angus Scale(SAS), Barnes Akathisia Rating Scale(BARS). Abnormal Involuntary Movement Scale(AIMS), and Liverpool University Neuroleptic Side Effect Rating Scale(LUNSERS). Results: 103 patients were switched to olanzapine in this study. The comparison between two switching methods did not show any significant difference in the dosage of olanzapine used, the concomitant use of benzodiazepine, the rate and reasons of drop-out, the adverse events, vital signs, laboratory tests, and most scales for measuring side-effects. However, the decrease in AIMS scores was significantly lower in ’direct switching method’ group, and the concomitant use of anticholinergics was comparatively greater in ’start-tapering switching method’ group. At baseline, SAS and BARS scores were 3.5 and 1.8 points respectively, and more than 70% of the subjects showed hyperprolactinemia. After switching to olanzapine, SAS, BARS, and AIMS scores were significantly decreased and the proportion of the patients with hyperprolactinemia was also decreased to less than 30%. However significant weight gain after the treatment of olanzapine was observed regardless of switching method. Conclusion: This study may suggest that switching to olanzapine can be done with relatively high safety regardless of switching methods and olanzapine can significantly decrease some side-effects induced by other antipsychotics.

만성중이염의 골도청력변화

박선홍,노용훈,이도용,나한조,김용기,최봉남,도남용 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

We have usually experienced bone conduction impairment in chronic otitis media, but it is unclear that raised bone conduction threshold is due to the reflection of cochlear dam- age and / or Carhart effect ( an artificial elevation of bone conduction threshold due to conductive defect ) or both. The purpose of this study is to verify the influence of the artificial elevation of the bone conduction threshold in chronic otitis media and to estimate Carhart effect. A total of 336 cases of tympanoplasty with unilateral chronic otitis media without any other causes of sensorineural hearing loss was studied. The average threshold levels of the bone conduction in diseased ears ranged from 18.5dB at 4kHz to 12.1dB at 250Hz The longer duration of the disease, the larger perforation size, the more severe damage of the middle ear structure and the presence of the cholesteatoma made bone conduction threshold increased. The postoperative thresholds of bone conduction were Improved in 36 cases (40.4%) of the 89 cases that underwent operation with type I tympanoplasty technique without electrie drilling. The degree of the maximal improvement was 14.3dB at 2000Hz and the minimum was 8.8dB at 250Hz. but these result did differ from a statistically significant degree. The result suggest that the bone conduction threshold in chronic otitis media is elevated artificially by conductive defect and improved by successful reconstructive surgery.

$\Theta$-Derivations on Prime Rings

Kyoo-Hong Park,Yong-Soo Jung 한국전산응용수학회 2003 Journal of applied mathematics & informatics Vol.12 No.1-2

In this paper we show the following: Let R be a prime ring (withcharacteristic dierent wo) and a ∈R. Let Θ, :R → R be automorphisms andletd :R → R be a nonzero Θ-derivation. (iif [d(x), a]Θ = 0 (or d([x, a]) = 0 )for all x ∈R, then a + (a) ∈Z, the center ofR, (iiif .d(x), a. = 0 for allx ∈R, then d(a) = 0. (iiiif [ ad(x), x] = 0 for all x ∈R, then eithera = 0 o rR is commutative.AMS Mathematics Subject Classication : 16W25, 16W20, 16U80Key words and phrases : Prime ring, Θ-derivation1. IntroductionThroughout this paper,R will represent an associative ring andZ will be itscenter. Letx,y∈R. The commutatorxy yx will be denoted by [x, y] and theproductxy+ yx by .x, y.. Let:R → R be an automorphism. We write [x, y]forxy (y)x, and will make extensive use of the following basic commutatoridentities: [xy, z] = x[y, z] + [x, z]y, [xy, z] = x[y, z]+ [x, (z)]y =x[y, z]+[x, z]y, [x, yz]=[x, y]z + (y)[x, z].Let Θ :R → R be an automorphism. An additive mapping d :R → R iscalled a Θ-derivation ifd(xy)=d(x)y ( x)d(y) for all x, y∈R.A 1-derivation is called simply a derivation, where 1 :R → R is the identitymap. A derivationd is inner if there exists ana ∈R such thatd(x)=[x, a] forall x ∈R.LetR be a prime ring with characteristic dierent wo andd : R → R anonzero derivation. In [1], I. N. Herstein proved that ifa ∈R and [d(x), a]=0Received July 10, 2002. Revised January 23, 2003This work was done under the support of Seowon University Research Fund 2002.c. 2003 Korean Society for Computational & Applied Mathematics.313314 Kyoo-Hong Park and Yong-Soo Jungfor allx ∈R, thena ∈Z. As a continuation of our earlier works for derivations([2], [3]), we here improve the above result and obtain some analogous results.

음식물류폐기물 공공 자원화시설 운영에 관한 연구 (Ⅰ)

홍용표ㆍ김혜선ㆍ김웅용ㆍ신현곤(Yong Pyo HongㆍHye Sun KimㆍUng Yong KimㆍHyun Gon Shin) 유기성자원학회 2016 유기물자원화 Vol.24 No.1

본 연구는 음식물류 폐기물 공공 자원화 시설 운영의 문제점과 제도 개선방안을 위하여 실시하였다. 기존 음식물류 폐기물 공공자원화 시설에 대한 실태조사를 통해 운영에 따른 문제를 분석하였으며, 이를 위하여 음식물류 폐기물의 발생량 및 자원화 현황, 공공 및 민간 자원화시설 현황, 전국의 주요 음식물류 폐기물 공공자원화 시설의 실제 운영과정에서 나타난 문제점을 분석한 결과는 다음과 같다: 현재 국내 음식물류 폐기물의 분리 수거량은 약 97%인 것으로 나타났다. 그리고 대부분의 공공 자원화시설은 음식물류 폐기물의 자원화가 아닌 단순처리에 급급한 것으로 나타났으며 경제성도 없는 것으로 분석되었다. 특히, 바이오가스시설 대부분은 실시설계와는 달리 가스 생산량 및 수요량이 전혀 맞지 않는 것으로 나타났다. This study is conducted to find out problems of the public food waste recycling facility and its improvement. Through a research on the actual condition, it is possible to analyze the problem of operation. Moreover, for this improvement, with analysis of the current state of recycling rate including its generation and the problem that can be shown from the real operation of the public/private food waste recycling facility, the results are as follows: It can be shown that the current amount of domestic food waste resource recycling is about 97 %. Almost every public recycling facility is analyzed to be economically infeasible and is not for recovery but to simple disposal. Especially, most of Biogas facilities appeared that amount of production and demand is not appropriate differed from enforcement design.