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( Yong-fei Zhou ),( Jiao-jiao Nie ),( Chao Shi ),( Ke Ning ),( Yu-feng Cao ),( Yanbo Xie ),( Hongyu Xiang ),( Qiuhong Xie ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10
COVID-19 is an emerging disease that poses a severe threat to global public health. As such, there is an urgent demand for vaccines against SARS-CoV-2, the virus that causes COVID-19. Here, we describe a virus-like nanoparticle candidate vaccine against SARS-CoV-2 produced by an E. coli expression system. The fusion protein of a truncated ORF2-encoded protein of aa 439~608 (p170) from hepatitis E virus CCJD-517 and the receptor-binding domain of the spike protein from SARSCoV-2 were expressed, purified and characterized. The antigenicity and immunogenicity of p170-RBD were evaluated in vitro and in Kunming mice. Our investigation revealed that p170-RBD selfassembled into approximately 24 nm virus-like particles, which could bind to serum from vaccinated people (p < 0.001) and receptors on cells. Immunization with p170-RBD induced the titer of IgG antibody vaccine increased from 14 days post-immunization and was significantly enhanced after a booster immunization at 28 dpi, ultimately reaching a peak level on 42 dpi with a titer of 4.97 log10. Pseudovirus neutralization tests showed that the candidate vaccine induced a strong neutralizing antibody response in mice. In this research, we demonstrated that p170-RBD possesses strong antigenicity and immunogenicity and could be a potential candidate for use in future SARS-CoV-2 vaccine development.
Insect-specific microRNA involved in the development of the silkworm Bombyx mori
Yong Zhang,Xue Zhou,Xie Ge,Jiang-Hao Jiang,Mu-Wang Li,Shi-Hai Jia,Xiao-Nan Yang,Yun-Chao Kan,Xue-Xia Miao,Guo-Ping Zhao,Fei Li,Yong-Ping Huang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. Insects are the largest group of animals and are extremely valuable in biological and agriculture research. Insects are also important pests to human health and agriculture, and efforts are necessary protect both humans and plants from disease and damage. Despite their importance, insects lag behind mammals, nematodes, and plants in miRNA research. At present, only 279 insect miRNAs have been identified from Drosophila melanogaster, Anopheles gambiae, Apis mellifera, Bombyx mori, and D. pseudoobscura in miRBase, and most of these miRNAs were computationally predicted without experimental validation. Functional analysis of insect miRNAs has only been conducted in D. melanogaster.
Han, Fei-Fei,Li, Liang,Shang, Bo-Yang,Shao, Rong-Guang,Zhen, Yong-Su Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Inhibition of heat shock protein 90 (Hsp90) leads to inappropriate processing of proteins involved in DNA damage repair pathways after DNA damage and may enhance tumor cell radio- and chemotherapy sensitivity. To investigate the potentiation of antitumor efficacy of lidamycin (LDM), an enediyne agent by the Hsp90 inhibitorgeldanamycin (GDM), and possible mechanisms, we have determined effects on ovarian cancer SKOV-3, hepatoma Bel-7402 and HepG2 cells by MTT assay, apoptosis assay, and cell cycle analysis. DNA damage was investigated with H2AX C-terminal phosphorylation (${\gamma}H2AX$) assays. We found that GDM synergistically sensitized SKOV-3 and Bel-7402 cells to the enediyne LDM, and this was accompanied by increased apoptosis. GDM pretreatment resulted in a greater LDM-induced DNA damage and reduced DNA repair as compared with LDM alone. However, in HepG2 cells GDM did not show significant sensitizing effects both in MTT assay and in DNA damage repair. Abrogation of LDM-induced $G_2/M$ arrest by GDM was found in SKOV-3 but not in HepG2 cells. Furthermore, the expression of ATM, related to DNA damage repair responses, was also decreased by GDM in SKOV-3 and Bel-7402 cells but not in HepG2 cells. These results demonstrate that Hsp90 inhibitors may potentiate the antitumor efficacy of LDM, possibly by reducing the repair of LDM-induced DNA damage.
Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12
Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.
Yong Sun,Jing Ping Zhang,Fei Guo,Lian Zhang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.9
Hydrochar was produced from hydrothermal treatment of corn straw black liquor. Response surface methodology (RSM) and the central composite design (CCD) were employed for determination of optimal char with maximum Pb2+ removal capacity. The operational parameters such as hydrothermal temperature (oC), duration (min) and solid liquid ratio (LSR) were chosen as independent variables in CCD. The statistical analysis indicates that the effects of hydrothermal temperature, duration, LSR and combined effect of hydrothermal temperature and duration are all significant for the Pb2+ removal capacity. The optimal condition for achieving the maximum Pb2+ adsorption capacity is obtained as the following: hydrothermal temperature (205 oC), duration (28min), LSR (12) with Pb2+ removal capacity reaching 47mg/g. The BET specific surface area of char prepared at optimal conditions could reach 85m2/g.
Yong Ding,Kebin Jiang,Fei Shao,Anzhong Deng 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.46 No.4
To have a better understanding of the torsional mechanism and influencing factors of PC composite box-girder with corrugated steel webs, ultimate torsional strength of four specimens under pure torsion were analyzed with Model Test Method. Monotonic pure torsion acts on specimens by eccentric concentrated loading. The experimental results show that cracks form at an angle of 45° to the member’s longitudinal axis in the top and bottom concrete slabs. Longitudinal reinforcement located in the center of cross section contributes little to torsional capacity of the specimens. Torsional rigidity is proportional to shape parameter η of corrugation and there is an increase in yielding torque and ultimate torque of specimens as the thickness of corrugated steel webs increases.
Fei Huang,Shuqing Li,Yanlin Zhao,Yong Liu 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.9
This paper presents an in-depth investigation into the transient impulsive pressure of an arc-curved water jet impacting a solid surface. The emphasis of this study is on the variations of the surface shape, which are classified into four types: The flat surface, the concave surface, the convex surface and the inclined surface. The numerical tool of arbitrary Lagrangian-Eulerian formulations is used to model the arc-curved jet impacting these different solid surface types. Elaborately designed experiments were conducted to test the impulsive pressure profile; the experimental results are found to be in approximate agreement with the numerical results. The impulsive pressure profiles of water jet impacting the flat and inclined solid surface are observed to exhibit two quintessential stages, in line with the traditional pressure profile; however, a double/multiple-peaked pressure structure is observed for the cases of the water jet impacting the concave and convex solid surfaces. Additionally, the value of the peak pressure is found to be a quadratic representation with the jet velocity, and the duration of the peak pressure is found to be an exponential representation with the jet velocity. The compression degrees of the liquid jet impacting the different surfaces are validated to be the root cause for the discrepancy of the impulsive pressure.
Yong-Peng Luo,Linkun Wang,Xu Guo,Jinlin Zheng,Fei-Yu Liao,Zixiong Guo 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8
This article proposes a structural damage identification method based on one-dimensional convolutional neural network group considering sensor faults. The method aims to reduce the damage misjudgment caused by sensor faults. In the proposed method, according to the sensor layout, some convolutional neural network sub-models are established to extract the features from raw vibration data for sensor fault diagnosis and structural damage identification; then two convolutional neural networks groups, namely the sensor fault diagnosis group and the damage identification group are designed on the basis of the functions of each sub-model. The sensor fault diagnosis group determines whether the sensor data is abnormal and truncates the abnormal signal. The remaining normal signal are entered into the damage identification group and the final damage identification results are calculated according to the statistical decision module. The effectiveness of the devised method is verified by the IASC–ASCE benchmark structure and laboratory experiments. The results demonstrate that the sensor fault diagnosis and damage identification accuracy of each sub-model ranges from 98.54% to 99.77% and from 87.21% to 91.74% respectively at different noise levels; the damage identification group can reduce the impact of sub-model misjudgment on the structural damage identification. The accuracy of the final damage identification results is 100%. The identification time of all samples in the test set is 53.09 s and 22.93 s, respectively, for SHM benchmark and Laboratory experiment cases. And the average judgment time of each submodel in the sensor fault diagnosis group was 278 and 94 ms, and that of each submodel in the damage identification group was 294 and 105 ms, respectively, for a single test sample, which fulfills the requirements of online damage identification for structural health monitoring.
Dynamic Characteristics of Saturated Soft Clays under Cyclic Loading in Drained Condition
Yong-hong Miao,Ruo-Yu Sheng,Jie Yin,Fan-Bo Zhou,Jian-fei Lu 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2
This study presents an experimental investigation on the effects of cyclic loading waveform and cyclic stress ratio (CSR) on dynamic behaviors through laboratory tests on a source of soft clay under cyclic loading in drained condition. Test results showed that under different cyclic loading waveforms at a given frequency (f = 1 Hz) and CSR = 0.6, the axial strain increases significantly at a relatively lower number of cycles (N) and then became stable, while the axial strain consistently decreases with the increasing N and tend to be stable. At a given N, specimen under sine wave cyclic loads shows a higher axial strain rate than that under triangle wave or square cyclic loads. Test results also indicated that at different CSRs, the axial strain increases rapidly at a relatively lower N and then gradually tends to be stable with the increasing N at lower CSR yet there is no stable trend for specimens at higher CSR, which indicates there is a critical value of CSR. It is recommended to apply the cyclic loading in sine waveform with CSR close to and less than the critical value to get a better treatment effect.