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( Hyung Sik Kim ),( Min Young Park ),( Sung Kyun Lee ),( Joon Seong Park ),( Ha Young Lee ),( Yoe-sik Bae ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.8
Emergency granulopoiesis is a very important strategy to supply efficient neutrophil number in response to infection. However, molecular mechanism involved in this process remains unclear. Here, we found that administration of WKYMVm, an immune modulating peptide, to septic mice strongly increased neutrophil number through augmented emergency granulopoiesis. WKYMVm-induced emergency granulopoiesis was blocked not only by a formyl peptide receptor 2 (FPR2) antagonist (WRW4), but also by FPR2 deficiency. As progenitors of neutrophils, Lin<sup>-</sup>c-kit<sup>+</sup>Sca-1<sup>-</sup> cells expressed FPR2. WKYMVm-induced emergency granulopoiesis was also blocked by a phospholipase C inhibitor (U-73122). These results suggest that WKYMVm can stimulate emergency granulopoiesis via FPR2 and phospholipase C enzymatic activity. [BMB Reports 2018; 51(8): 418-423]
Tae, Gun-Sik,Jeong, Kyoung-Yong,Bang, In-Seok,Yoe, Sung-Moon 한국곤충학회 1998 Entomological Research Vol.28 No.3
E. coli K12로 면역시킨 배추흰나비 유충의 지방체로부터 항균 펩티드와 유사한 단백질을 암호하는 3종류의 cDNA를 부분적으로 클로닝하였다. 이들 유전자의 염기서열은 서로 매우 유사하였으며 일차구조 분석 결과 연역된 아미노산 서열은 cecropin류의 항균단백질의 특성을 나타내었다. 그리고, 나방류와 파리류에서 밝혀진 항균단백질에서 잘 보존된 모든 아미노산이 새로이 클로닝된 단백질들에서도 모두 잘 보존되어 있음이 확인되었다. Three cDNA clones encoding the partial sequence of the antimicrobial factor-like peptide were isolated from the fat body of the cabbageworm, Artogeia rapae immunized with E. coli K12. Nucleotide sequences of three cloned genes have a high degree of homology one another. The primary structure analysis revealed that their deduced amino acid sequences showed the characteristics of the cecropin family antibacterial peptides and that the amino acid residues which are highly conserved in the antibacterial peptides from moths and flies are also conserved in butterfly, implying that these clones are encoding the antibacterial peptides.
Eun-Jung Lim,Yoe-Sik Bae,백석환,Dae-Weon Park,Jin-Gu Lee,이추희,Young-Chul Hwang,Jae-Weon Jeong,진병로 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.10
Synthetic oligodeoxynucleotides (ODN) with a CpGmotif are recognized by Toll-like receptor 9 (TLR9) and pleiotropic immune responses are elicited. Stimulation of macrophages with TLR9 agonist prevented apoptosis induced by serum deprivation through increased expression of FLICE-like inhibitory protein (FLIP). CpG ODN-mediated anti-apoptosis depended on the TLR9-Akt-FoxO3a signaling pathway. Inhibition of TLR9 by small interfering (si) RNA or an inhibitor suppressed CpG ODN-mediated anti-apoptosis. Analysis of signaling pathways revealed that the anti-apoptotic effect of CpG ODN required phosphorylation of FoxO3a and its translocation from the nucleus to the cytosol. Overexpression of FoxO3a increased apoptosis induced by serum deprivation and CpG ODN blocked these effects through FLIP expression. In contrast,siRNA knock-down of FoxO3a decreased apoptosis by serum deprivation. In addition, Akt activation was involved in CpG ODN-induced phosphorylation of FoxO3a, expression of FLIP, and anti-apoptosis. Taken together, these results demonstrate the involvement of Akt-FoxO3a in TLR9-mediated anti-apoptosis and indicate that FoxO3a is a distinct regulator for FLIP expression.
Kim, Jin‐,Sik,Yeo, Seungeun,Shin, Dong‐,Gu,Bae, Yoe‐,Sik,Lee, Jae‐,Jin,Chin, Byung‐,Rho,Lee, Chu‐,Hee,Baek, Suk‐,Hwan Blackwell Publishing Ltd 2010 FEBS JOURNAL Vol.277 No.13
<P>Macrophage activation contributes to the pathogenesis of atherosclerosis. In the vascular system, the major source of reactive oxygen species is the NADPH oxidase (Nox) family. Nox1 is induced by lipopolysaccharide (LPS) in macrophages, but the expression mechanism is not fully understood. We found that LPS causes β‐catenin accumulation by glycogen synthase kinase 3β (GSK3β) inactivation, and that β‐catenin accumulation increases Nox1 expression. LPS induced Nox1 mRNA expression and reactive oxygen species generation in Raw264.7 cells. Using bone marrow‐derived macrophages from toll‐like receptor 4 mutant mice, we also tested whether LPS‐induced Nox1 expression is toll‐like receptor 4 dependent. LPS caused GSK3β phosphorylation, induced β‐catenin accumulation and increased nuclear translocation. The GSK3β inhibitor LiCl potentiated LPS‐induced Nox1 expression in accordance with β‐catenin accumulation and nuclear translocation. Conversely, ectopic expression of a constitutively active GSK3β mutant severely attenuated Nox1 expression. These findings identify a novel regulatory pathway controlling Nox1 expression by LPS‐stimulated macrophages.</P>
최정식,여성문,김웅석,채권석,Choi, Chung-Sik,Yoe, Sung-Moon,Kim, Eung-Seok,Chae, Kwon-Seok,Kim, Hak R. The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.3
Antibacterial activity was induced in the haemolymph of the common cutworm, Spodoptera litura by the artificial injection of E, coli Ek132. Antibacterial peptides were purified from the immunized haemolymph by heat treatment, ion-exchange chromatography, gel filtration chromatography, and reverse phase FPLC, and their physicochemical characteristics were investigated. These purified antibacterial peptides designated as spodopsin la and Ib were named after Spodoptera litura. Spodopsin la and Ib had the apparent molecular masses of 3, 823 Da and 3, 886 Da, respectively, and about 20% of the sequences had basic amino acids, such as lysine and arginine but no cysteine. Also, spodopsin l was confirmed to be a new member of cecropin family having a similar amino acid sequence to cecropin of lepidopteran insects, such as Bombyx mori and Hyalophora cecropia. The purified spodopsin was active against gram-positive as well as gram-negative bacteria.
Sphingosylphosphorylcholine stimulates human monocyte-derived dendritic cell chemotaxis
LEE, Ha-young,SHIN, Eun-ha,BAE, Yoe-sik Springer Science and Business Media LLC 2006 Acta pharmacologica Sinica. Vol.27 No.10
<P>Aim: To investigate the effects of sphingosylphosphorylcholine (SPC) on human monocyte-derived dendritic cell (DC) chemotaxis. Methods: Human DC were generated from peripheral blood monocytes by culturing them with granulocyte macrophage-colony stimulating factor and interleukin-4. The effect of SPC on the DC chemotactic migration was measured by chemotaxis assay. Intracellular signaling event involved in the SPC-induced DC chemotaxis was investigated with several inhibitors for specific kinase. The expression of the SPC receptors was examined by reverse transcription polymerase chain reaction. Results: We found that SPC induced chemotactic migration in immature DC (iDC) and mature DC (mDC). In terms of SPC-induced signaling events, mitogen activated protein kinase activation and Akt activation in iDC and mDC were stimulated. SPC-induced chemotaxis was mediated by extracellular signal-regulated protein kinase and phosphoino-sitide-3-kinase, but not by calcium in both iDC and mDC. Although mDC express ovarian cancer G protein-coupled receptor 1, but not G protein-coupled receptor 4, iDC do not express any of these receptors. To examine the involvement of sphingosine-1-phosphate (S1P) receptors, we checked the effect of an S1P receptor antagonist (VPC23019) on SPC-induced DC chemotaxis. VPC23019 did not affect SPC-induced DC chemotaxis. Conclusion: The results suggest that SPC may play a role in regulating DC trafficking during phagocytosis and the T cell-stimulating phase, and the unique SPC receptor, which is different from S1P receptors, is involved in SPC-induced chemotaxis.</P>
이용웅 ( Lee Yong Woong ),조종식 ( Cho Jong Sik ),주종길 ( Ju Jong Gil ),신창선 ( Shin Chang Sun ),여현 ( Yoe Hyun ),이종현 ( Lee Jong Hyun ),신한호 ( Sin Han Ho ),염창열 ( Yum Chang Yeol ) 한국농공학회 2010 한국농공학회논문집 Vol.52 No.2
In this paper, we propose a ubiquitous Farming Diary System which can support the easy and reliable recording of a farming diary for the certificate on environment-friendly agricultural products by using the USN(Ubiquitous Sensor Network) technologies. By using growth-related data, the system can also control farming facilities remotely and automatically. To achieve this goal, the UFDS(Ubiquitous Farming Diary System) is consisted with 3 layers. The first ‘physical layer’ can collect data from sensors, cameras and facilities then controls the growth environment based on the analyzed information. The second ‘Middle layer’ can process and store the data from ‘physical layer’ to sensor manager, image manager, control manager and diary manager separately. The third ‘application layer’ can provide growth-related services to users through various applications. The UFDS can recording grow history information automatically and Easily. Besides, the system can make an accurate and reliable farming diary with multimedia information such as motion and sound. Furthermore, environmental information such as temperature, humidity, luminance and soil conditions (soil temperature, soil humidity, soil EC) can be monitored in real-time and the facilities managed in remote sites.
Shin, Myeong Heon,Lee, Young-Ah,Bae, Yoe-Sik,Kita, Hirohito,Kim, Youngdong,Ryu, Sung Ho S. Karger AG 2005 International archives of allergy and immunology Vol.137 No.suppl1
<P><I>Background:</I> Eosinophils play a key role in allergic inflammation and parasitic infections. The synthetic peptide, Trp-Lys-Tyr-Met-Val-<I>D</I>-Met (WKYMVm), has been previously shown to activate eosinophils and thus to enhance respiratory burst through the formyl peptide receptors. <I>Objective:</I> This study was undertaken to determine the intracellular signaling pathway involved in WKYMVm-stimulated superoxide production by human eosinophils. <I>Methods:</I> Purified eosinophils from peripheral blood were stimulated with various concentrations (10<SUP>-3</SUP> to 10 μ<I>M</I>) of WKYMVm and the involvement of PI3-kinase and MAP kinases in WKYMVm-triggered superoxide production was investigated using pharmacological inhibitors. <I>Results:</I> WKYMVm-induced superoxide production by eosinophils was strongly inhibited by pretreatment with the PI3-kinase inhibitor LY294002. In addition, pretreatment with the ERK1/2 kinase inhibitor PD98059 resulted in marked inhibition of superoxide production induced by WKYMVm. Indeed, WKYMVm strongly induced phosphorylation of ERK1/2. The ERK1/2 activation by the peptide was transient and peaked after 2 min of stimulation. Furthermore, ERK1/2 activation by WKYMVm was completely inhibited by pretreatment with the PI3-kinase inhibitor LY294002, but not by the PKC inhibitor Ro-31-8220. <I>Conclusion:</I> These results suggest that WKYMVm stimulates human eosinophils to induce superoxide production via a PI3-kinase-mediated ERK1/2 pathway.</P><P>Copyright © 2005 S. Karger AG, Basel</P>