Silencing MR-1 attenuates atherosclerosis in ApoE–/– mice induced by angiotensin II through FAK-Akt –mTOR-NF-kappaB signaling pathway

Yixi Chen,Jianping Cao,Qihui Zhao,Haiyong Luo,Yiguang Wang,Wenjian Dai 대한생리학회-대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.2

Myofibrillogenesis regulator-1 (MR-1) is a novel protein involved in cellular proliferation, migration, inflammatory reaction and signal transduction. However,little information is available on the relationship between MR-1 expression and the progression of atherosclerosis. Here we report atheroprotective effects of silencing MR-1 in a model of Ang II-accelerated atherosclerosis, characterized by suppression focal adhesion kinase (FAK) and nuclear factor kappaB (NF-κB) signaling pathway,and atherosclerotic lesion macrophage content. In this model, administration of the siRNA-MR-1 substantially attenuated Ang II-accelerated atherosclerosis with stabilization of atherosclerotic plaques and inhibited FAK, Akt, mammalian target of rapamycin (mTOR) and NF-kB activation, which was associated with suppression of inflammatory factor and atherogenic gene expression in the artery. In vitro studies demonstrated similar changes in Ang II-treated vascular smooth muscle cells (VSMCs) and macrophages: siRNA-MR-1 inhibited the expression levels of proinflammatory factor. These studies uncover crucial proinflammatory mechanisms of Ang II and highlight actions of silencing MR-1 to inhibit Ang II signaling, which is atheroprotective.

Dye Adsorption from Aqueous Solution by Cellulose/Chitosan Composite: Equilibrium, Kinetics, and Thermodynamics

Yixi Wang,Hao Wang,Huili Peng,Zhicun Wang,Jianning Wu,Zhiyong Liu 한국섬유공학회 2018 Fibers and polymers Vol.19 No.2

A novel eco-friendly porous adsorbent of cellulose (CE)/chitosan (CS) aerogel was prepared through sol-gel process and freeze-drying to remove Congo Red (CR). A series of aerogels were prepared by adjusting the mass ratios of CE and CS. Composite aerogels were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). SEM images showed that it was possible to change the structure of the aerogel by adjusting the amount of chitosan. The effects of dosage of chitosan, initial pH, temperature, adsorbent dosage, contact time, and initial dye concentration on adsorption capacities for CR were studied in detail. Batch adsorption studies showed that aerogel exhibited maximum removal efficiency to CR at a composite ratio of 1:3 and dosage of 2.5 g/l. CE/CS aerogel had excellent adsorption capacities for CR at a pH range of 3-11, which indicated stability of the aerogel in both acidic and alkaline conditions. CR adsorption on the composite aerogel fitted pseudo-second-order kinetics and Langmuir isotherm. The Langmuir isotherm model revealed that the maximum theoretical adsorption capacity of this material for CR was 381.7 mg/g at pH 7.0 at 303 K for 24 h. The adsorption mechanism included electrostatic and chemical interactions. The results indicated that the adsorption capacity of CE/CS aerogels was higher than the other chitosan composites adsorbents.

GAN생성 경영교육 콘텐츠의 학습효과에 대한 연구

동혁희(Yixi Dong),권오병 한국지능정보시스템학회 2022 한국지능정보시스템학회 학술대회논문집 Vol.2022 No.6

Silencing MR-1 attenuates atherosclerosis in ApoE^-/- mice induced by angiotensin II through FAK-Akt -mTOR-NF-kappaB signaling pathway

Chen, Yixi,Cao, Jianping,Zhao, Qihui,Luo, Haiyong,Wang, Yiguang,Dai, Wenjian The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.2

Myofibrillogenesis regulator-1 (MR-1) is a novel protein involved in cellular proliferation, migration, inflammatory reaction and signal transduction. However, little information is available on the relationship between MR-1 expression and the progression of atherosclerosis. Here we report atheroprotective effects of silencing MR-1 in a model of Ang II-accelerated atherosclerosis, characterized by suppression focal adhesion kinase (FAK) and nuclear factor kappaB ($NF-{\kappa}B$) signaling pathway, and atherosclerotic lesion macrophage content. In this model, administration of the siRNA-MR-1 substantially attenuated Ang II-accelerated atherosclerosis with stabilization of atherosclerotic plaques and inhibited FAK, Akt, mammalian target of rapamycin (mTOR) and NF-kB activation, which was associated with suppression of inflammatory factor and atherogenic gene expression in the artery. In vitro studies demonstrated similar changes in Ang II-treated vascular smooth muscle cells (VSMCs) and macrophages: siRNA-MR-1 inhibited the expression levels of proinflammatory factor. These studies uncover crucial proinflammatory mechanisms of Ang II and highlight actions of silencing MR-1 to inhibit Ang II signaling, which is atheroprotective.

EFFECT OF POLYOXYMETHYLENE DIMETHYL ETHERS/DIESEL BLENDS ON FUEL PROPERTIES AND PARTICULATE MATTER OXIDATION ACTIVITY OF A LIGHT-DUTY DIESEL ENGINE

Jing Tian,Yixi Cai,Yunxi Shi,Yingxin Cui,Runlin Fan 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.2

Polyoxymethylene dimethyl ethers (PODE) was blended in diesel at volume ratios of 0 %, 10 %, 20 %, and 30 % (denoted as P0, P10, P20, and P30). The experimental study was carried on an unmodified YD480Q diesel engine. An engine exhaust particle sizer was introduced to analyze the particulate matter (PM) concentration and particle size distribution of diesel engine emission. The evaporation-oxidation characteristics of the PODE/diesel blends and the effect of the fuel blends on the oxidative activity of PM were investigated by thermogravimetric analysis and Arrhenius theorem. The results showed that blending PODE in diesel improved the evaporation-oxidation characteristics of the fuel and decreased the apparent activation energy of the fuel blends. PODE played a positive role in reducing PM emissions. The particle total number concentration of P30 decreased 28.29 ~ 66.60 % and the particle total volume density decreased 54.16 ~ 80.06 % compared to diesel. The particle size distribution shifted to a smaller particle size as the PODE blending ratio was increased. The mass fraction of the volatile substances (VS) increased and the mass fraction of the dry soot (DS) decreased by employing PODE as a diesel additive. Also, the oxidation activity of VS increased as the PODE blending ratio was increased. The oxidation activity of DS climbed to the peak when the PODE blending ratio was 20 % and then decreased.

EFFECTS OF RESIDUAL ASH ON DPF CAPTURE AND REGENERATION

Yingxin Cui,Yixi Cai,Runlin Fan,Yunxi Shi,Linbo Gu,Xiaoyu Pu,Jing Tian 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.5

To study the effects of residual ash on the capture and regeneration of a diesel particulate filter (DPF), repeated capture and complete regeneration experiments were conducted. An engine exhaust particulate sizer was used to measure the particle size distribution of diesel in the front and back of DPF. Discrepancies in the size distribution of the particulate matter in repeated trapping tests were analyzed. To achieve complete DPF regeneration, a DPF regeneration system using nonthermal plasma technology was established. The regeneration carbon removal mass and peak temperatures of DPF internal measuring points were monitored to evaluate the effect of regeneration. The mechanism explaining the influence of residual ash on DPF capture and regeneration was thoroughly investigated. Results indicate that the DPF trapping efficiencies of the nuclear-mode particles and ultrafine particles have significant improvements with the increase quantity of residual ash, from 90 % and 96.01 % to 94.17 % and 97.27 %, respectively. The exhaust backpressure of the DPF rises from 9.41 kPa to 11.24 kPa. Heat transfer in the DPF is improved with ash, and the peak temperatures of the measuring points accordingly increase. By comparing the regeneration trials, the elapsed time for complete regeneration and time difference for reaching the peak temperature between adjacent reaction interfaces are extended with increased quantity of ash. The carbon removal mass rises by 34.00 %.

Experimental studies on the diesel engine urea-SCR system using a double NOx sensor system

Wei Tang,Yixi Cai,Jun Wang 대한환경공학회 2015 Environmental Engineering Research Vol.20 No.4

SCR has been popularly approved as one of the most effective means for NOx emission control in heavy-duty and medium-duty vehicles currently. However, high urea dosing would lead to ammonia slip. And NH₃ sensor for vehicle emission applications has not been popularly used in real applications. This paper presents experimental studies on the diesel engine urea-SCR system by using a double NOx sensor system that is arranged in the downstream of the SCR catalyst based on ammonia cross-sensitivity. It was shown that the NOx conversion efficiency rised as NH₃/NOx increases and the ammonia slip started from the NH₃/NOx equal to 1.4. The increase of temperature caused high improvement of the SCR reaction rate while the space velocity had no obvious change. The ammonia slip was in advance as catalyst temperature or space velocity increase and the ammonia storage reduced as catalyst temperature or space velocity increase. The NOx real-time conversion efficiency rised as the ammonia accumulative storage increase and reached the maximum value gradually.

CARBON DEPOSIT INCINERATION DURING ENGINE FLAMEOUT USING NON-THERMAL PLASMA INJECTION

Xiaoyu Pu,Yixi Cai,Yunxi Shi,Jing Wang,Linbo Gu,Jing Tian,Runlin Fan 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.3

−In order to investigate the influence of initial regeneration temperatures on diesel particulate filter (DPF) regeneration, an experimental study of DPF regeneration was implemented using a dielectric barrier discharge (DBD) reactor, aided by exhaust waste heat after engine flameout. DPF trapping characteristics and carbon deposit mass were discussed to facilitate further data analysis and calculation. DPF regeneration was then investigated by comparison analysis of deposit removal mass, backpressure drop, and internal temperature change. The results revealed that a large amount of particulate matter (PM) was deposited in DPF with a high filtration efficiency of about 90 %. The deposit removal rate and percentage drop of the backpressure both maximized at the initial temperature of 100 oC. During DPF regeneration, the sharp rise of internal temperature indicated vigorous PM incineration and high CO2 emission. The results successfully demonstrated DPF regeneration using non-thermal plasma injection during engine flameout, and prominent heat durability was achieved in this method.

Coking characteristics and deactivation mechanism of the HZSM-5 zeolite employed in the upgrading of biomass-derived vapors

Yongsheng Fan,Yixi Cai,Xiaohua Li,Haiyun Yin,Jisheng Xia 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.46 No.-

HZSM-5 zeolite was employed in the upgrading of biomass pyrolysis vapors in this study. The cokingcharacteristics were investigated by means of TG, FT-IR, XRD, N2 adsorption–desorption, NH3-TPD, Py-IR,SEM and TEM methods. When the zeolite was used for three times about 120 min, its activity had beenlost and the amount of coke was 12.15% composed of 9.90% of I typefilamentous coke and 2.25% of II typegraphite-like coke. The skeleton structure of zeolite was basically intact, which had not been seriouslydestroyed by coke. The surface area, pore volume and acidity of zeolite were all deteriorated vary degreeswith the usage time. The zeolite granules became larger after deactivated, and thefilamentous coke canbe observed both on the surface and in the pore. The compositions of the coke precursors were analyzedby FT-IR and GC/MS. The precursors of coke deposited in the pore were mainly aromatic hydrocarbons,while the species of the precursors deposited on the outer surface was more including many long-chainsaturated hydrocarbons. The deactivation of HZSM-5 zeolite began from inner, large molecularsubstances blocked the pores which resulted in the zeolite deactivation eventually

Design of Non-fragile Robust H<SUB>∞</SUB> Filter for Fuzzy Stochastic Systems with Time-Varying Delay

HUA Mingang,CAI Yixi,TAN Huasheng 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10

This paper concerns the design of the non-fragile H∞ filter for the fuzzy system with time-varying delays. Attention is focused on the design of the filter which is subject to gain variations, such that the filtering system is robustly stable with a prescribed H∞ performance level for all admissible uncertainties. A delay-dependent sufficient condition for the existence of such filters is obtained in terms of a linear matrix inequality, and a numerical example is given to proof the effectiveness of the method.