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Yin Zhou,Tong Wen,Xia Chen,Longqin Liu,Fan Yang,Yu Zheng 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.8
Structures with curved-holes are of great value for industrial application, but how to realize the formation of integral curved hole is still a huge challenge. In the current study, a novel methodology, called Bulk Forming of Curved Hole (BFCH), was proposed to obtain integral structures with curved holes by combining traditional drilling and plastic forming processes. An analytic formula of cross-section distortion in BFCH was derived based on the upper bound method. The formula shows the influence of three factors, including the bending angle θ, billet thickness-to-hole diameter ratio h/d , and material properties index Øk related to material strength, on the wall collapse of the curved hole formed. Moreover, three types of curved holes were designed to verify the concept, and the results of experiments and fi nite element simulation using Al6061 specimens proved that the method is feasible to form integral structures with inside curved-holes. The oval-shaped distortion of the hole cross-sections is the main defect, which increases with the bending angle θ , as well as the value of h/d and Øk. In addition, proper initial temperature difference inside the billet can decrease the distortion of the formed holes.
Site-specific deletions in the tomato genome by the CinH-RS2 and ParA-MRS recombination systems
Yin Zhou,Ying Wang,Yuan-Yeu Yau,David W. Ow 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
We have tested the CinH-RS2 and ParA-MRS site-specific deletion systems in tomato (Solanum lycopersicum L.). The ParA-MRS system is derived from the broad-host-range plasmid RK2, where the 222 aa ParA recombinase recognizes a 133 bp multimer resolution site (MRS). The CinH-RS2 system is derived from Acinetobacter plasmids pKLH2 and pKLH204, where the 188 amino acid CinH recombinase recognizes a 113-bp recombination site known as RS2. In this study, target lines containing a DNA segment flanked by recombination sites were crossed to recombinase-expressing lines producing CinH or ParA recombinase. CinH-mediated recombination of RS2 substrates was detected in 2 of 3 F1 plants that harbor both the target and recombinase loci. On the other hand, recombination mediated by ParA was not detected among F1 plants,but was found among 13 of 47 F2 plants. These data show that both systems can mediate site-specific DNA deletion in the tomato genome, and, upon further refinement, can provide additional molecular tools for tomato improvement through precise genome manipulation. As the target construct also contains additional recombination sites for sitespecific integration by other recombination systems, these tomato lines could be used for future testing of gene stacking through site-specific integration.
A Distributed Workflow Mapping Method Oriented Homogeneous Activity
Fei Yang,Yinzhou Zhu 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.3
Workflow model based-on homogeneous activity needs to be transformed into basic workflow model in order to run in distributed environment. A workflow model mapping algorithm oriented homogeneous activity is proposed which can generate independent basic workflow fragments for homogeneous activities of different configuration modes using different processing mechanisms, and then form the basic workflow model based on the assembly of these workflow fragments. The algorithm provides a complete set of virtual mapping mechanism to solve it effectively focusing on the problem of the topological relationship of redundant activities difficult to describe in the dynamic selection model of homogeneous activity.
Liu Yize,Qiu Guanzhen,Luo Yinzhou,Li Shanshan,Xu Yeqiu,Zhang Yuanzhuang,Hu Jiayuan,Li Peifeng,Pan Hai,Wang Yong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
As the most prevalent bone tumor in children and adolescents, the pathogenesis and metastasis of osteosarcoma (OS) remain largely unclear. Here, we investigated the expression and function of a novel circular RNA (circRNA), circROCK1-E3/E4, which is back-spliced from exons 3 and 4 of Rho-associated coiled-coil containing protein kinase 1 (ROCK1) in OS. We found that circROCK1-E3/E4, regulated by the well-known RNA-binding protein quaking (QKI), was downregulated in OS and correlated with unfavorable clinical features of patients with OS. Functional proliferation and cell motility assays indicated that circROCK1-E3/E4 serves as a tumor suppressor in OS cells. Mechanistically, circROCK1-E3/E4 suppressed proliferation and migration by upregulating phosphatase and tensin homolog (PTEN) through microRNA-532-5p (miR-532-5p) sponging. In the constructed nude mouse model, circROCK1-E3/E4 inhibited tumor growth and lung metastasis in vivo. This study demonstrates the functions and molecular mechanisms of circROCK1-E3/E4 in the progression of OS. These findings may identify novel targets for the molecular therapy of OS.
Lina Xiong,Shuqin Mao,Baiyi Lu,Jiajia Yang,Fei Zhou,Yinzhou Hu,Yirong Jiang,Canxi Shen,Yajing Zhao 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.1
Osmanthus fragrans flower extract (OFE) is an organic extract from O. fragrans flower, which exhibits neuroprotective, free radical scavenging, and antioxidant effects. Therefore, the protective effect of OFE and acteoside against aging was studied. An aging ICR mouse model was established by chronically administering d-galactose (250 mg/kg) for 8 weeks. d-galactose induced spatial learning and memory impairments that were successfully inhibited by OFE and acteoside, which could shorten escape latency, improve platform crossing times, and increase zone time. The antioxidant potential of OFE and acteoside in vivo was evaluated by estimating the following: activities of antioxidant enzymes, such as glutathione peroxidase and aging-related enzyme, particularly monoamine oxidase; contents of lipid peroxidation methane dicarboxylic aldehyde, advanced glycation end products, and 8-hydroxy-2'-deoxyguanosine (a DNA damage product); and levels of nuclear factor-erythroid 2-related factor 2. OFE and acteoside also inhibited d-galactose-induced neurological aging by suppressing the increase in glial fibrillary acidic protein and neurotrophin-3. Considering the dose-dependent protective effects of OFE and acteoside, we concluded that OFE, rich in acteoside, was a good source of natural antiaging compounds.