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( Young Yil Bahk ),( Hyun Sook Kim ),( Ok-jae Rhee ),( Kyung-a You ),( Kyung Seon Bae ),( Woojoo Lee ),( Tong-soo Kim ),( Sang-seob Lee ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10
Climate change is expected to affect not only availability and quality of water, the valuable resource of human life on Earth, but also ultimately public health issue. A six-year monitoring (total 20 times) of Escherichia coli O157, Salmonella enterica, Legionella pneumophila, Shigella sonnei, Campylobacter jejuni, and Vibrio cholerae was conducted at five raw water sampling sites including two lakes, Hyundo region (Geum River) and two locations near Water Intake Plants of Han River (Guui region) and Nakdong River (Moolgeum region). A total 100 samples of 40 L water were tested. Most of the targeted bacteria were found in 77% of the samples and at least one of the target bacteria was detected (65%). Among all the detected bacteria, E. coli O157 were the most prevalent with a detection frequency of 22%, while S. sonnei was the least prevalent with a detection frequency of 2%. Nearly all the bacteria (except for S. sonnei) were present in samples from Lake Soyang, Lake Juam, and the Moolgeum region in Nakdong River, while C. jejuni was detected in those from the Guui region in Han River. During the six-year sampling period, individual targeted noxious bacteria in water samples exhibited seasonal patterns in their occurrence that were different from the indicator bacteria levels in the water samples. The fact that they were detected in the five Korea’s representative water environments make it necessary to establish the chemical and biological analysis for noxious bacteria and sophisticated management systems in response to climate change.
완두(Pisum sativum L.) 葉肉細胞 原形質體의 分雜 및 融合
權一瓚,金仁燮,金達雄 慶北大學校 農業科學技術硏究所 1988 慶北大農學誌 Vol.6 No.-
완두葉肉細胞의 原形質體分離 및 融合에 있어서 酵素의 處理時間, CaCl₂·2H₂O의 效果, 酸素의 濃度와 葉의 位置에 따른 原形質體의 分離에 미치는 影響과 Mole 濃度에 따른 遠心分離效果, PEG溶液의 處理時間, 酸度에 따른 融合效果 그리고 dimethyl sulfoxide 添加效果를 究明하기 위하여 實施한 實驗의 結果는 다음과 같다. 1. 原形質體의 分離에는 4時間의 酸素處理가 가장 良好했고 CaCl₂·2H₂O의 添加에 따라 原形質體의 分離速度가 늦어졌으며 活性度는 4時間까지 變動이 없었고 CaCl₂·2H₂O에 의해서 滑性度가 長期間 維持되었다. 2. 酸素의 濃度는 1%이상의 處理에서 거의 同一한 原形質體分離率을 나타냈으며 上位 1∼2葉이 가장 좋은 原形質體分離率을 보였으며 遠心分離效果는 0.4M, 0.5M에서 良好하였다. 3. 原形質體融合은 PEG 6000溶液 75分 處理에서 좋은 融合率을 보였다. 그러나 30分 以上의 處理가 活性度에 影響을 미치지 않았으며 pH 5.8인 PEG 6000의 0.2M溶液에서 가장 높은 62.84%의 融合率을 나타내었다. dimethyl sulfoxide에 의한 效果는 融合率이 3∼7% 減少하지만 binucleate의 誘道에 有效한 것으로 推定된다. This experiment was conducted to identify the enzyme treatment time, calcium ion effect, enzyme concentration and leaf position for protoplast isolation. It was also performed to determine the adequate molarity on protoplasts, and to investigate the incubation time, pH, PEG concentration and DMSO effect for protoplast fusion. The results obtained were summarized as follows; The optimal time of incubation in enzyme solution was 4 hours. And the protoplast releasing time was delayed by CaCl₂·2H₂O addition to the enzyme solution compared with no added one. The viability had kept up to above 95% until the 4 hours after digestion. The high viability of the protoplast was preserved more than 16 hours by adding CaCl₂·2H₂O to digestion solution. The enzyme concentration had no effect on protoplast yield in range from 1% to 5% and the first or second leaf from the top of the plant produced the highest protoplast yield among the leaf position tested. The purity of healthy protoplast was better in 0.4M and 0.5M sucrose than in others, and the percentage of protoplast aggregation was more 20% to 50% in PEG 6,000 compared with 4,000 and PEG 1,500. Even though the percentage of protoplast aggregation was less increased by 3% to 7% than without DMSO, its treatment was effective to induce binucleated protoplasts.
The Mechanism of Antibacterial Activity of Tetrandrine Against <i>Staphylococcus aureus</i>
Lee, Young-Seob,Han, Sin-Hee,Lee, Su-Hwan,Kim, Young-Guk,Park, Chung-Berm,Kang, Ok-Hwa,Keum, Joon-Ho,Kim, Sung-Bae,Mun, Su-Hyun,Seo, Yun-Soo,Myung, Noh-Yil,Kwon, Dong-Yeul Mary Ann Liebert 2012 Foodborne pathogens and disease Vol.9 No.8
( Woosung Hong ),( Yil-seob Lee ),( Chun-wook Park ),( Moon-soo Yoon ),( Young Suck Ro ) 대한피부과학회 2018 Annals of Dermatology Vol.30 No.4
Background: With the approval of topical retapamulin ointment in 2011, it was officially required to conduct a post-marketing surveillance (PMS) study to obtain further data of its safety profile and effectiveness, in accordance with the requirement of the Korean Ministry of Food and Drug Safety (MFDS). Objective: This study had prospectively designed to monitor safety and tolerability with the effectiveness of topical retapamulin in clinical practices. Methods: Open label, multi-center, non-interventional observational study was done from May 2011 to October 2015. All subjects had bacterial skin infections of locally approved prescribing information accordingly. The study mainly focused on safety issues in the local target population (3,612 eligible subjects). And, drug effectiveness was also evaluated by physicians. Results: The incidence of adverse events (AEs) and adverse drug reactions (ADRs) were 2.53% and 0.97%, respectively. In terms of the incidence of unexpected AEs and ADRs, 1.45% and 0.33%, and for the incidence of serious AEs, 0.28%, whereas no serious ADRs reported. And, the effectiveness of topical retapamulin rate was 96.1% (1,697 of total 1,765 subjects). Conclusion: Topical retapamulin is to be well-tolerated and effective in patients with bacterial skin infections of locally approved prescribing information. (Ann Dermatol 30(4) 441∼450, 2018)
Protective Effect of a Chrysanthemum indicum containing Formulation in Cadmium-Induced Ototoxicity.
Kim, Su-Jin,Myung, Noh-Yil,Shin, Bong-Gi,Lee, Jeong-Han,So, Hong-Seob,Park, Rae-Kil,Um, Jae-Young,Hong, Seung-Heon Institute for Advanced Research in Asian Science a 2011 The American journal of Chinese medicine Vol.39 No.3
<P>Chungshinchongyitang (CSCYT) is an herbal drug formula containing Chrysanthemum indicum and 13 other herbs used for treating auditory diseases. Irreversible hearing loss is a characteristic effect of a number of heavy metals. Cadmium (Cd(2+)) is an environmental contaminant that causes a variety of adverse effects. In the present study, we investigate the protective effects of CSCYT against Cd(2+) induced ototoxicity in vitro and ex vivo. The findings of this study show that CSCYT prevents the destruction of hair cell arrays induced by Cd(2+) in the rat organ of Corti primary explants. CSCYT inhibited cell death, release of cytochrome c and generation of reactive oxygen species induced by Cd(2+) in HEI-OC1 auditory cell line. In addition, we also demonstrated that CSCYT exerted its effect by modulating of apoptosis via the caspase-3 activation and extracellular signal-regulated kinase activation. These results are expected to improve the understanding of the pharmacological mechanism of CSCYT and aid in the development of potential therapeutic strategies against ototoxicity.</P>
정재훈(Jae-Hun Jeong),김일섭(Yil-Seob Kim) 전남대학교 수산과학연구소 2021 수산과학연구소논문집 Vol.30 No.2
It is essential to lessen the needed volume of the sand nourishment for the economic beach management. However, the uncertainty factors of seasonal weather and wave climate change hinder to save the budget for the beach management. To reduce the sand loss rate, one can think of ways to lessen the sand movement. If the predicted sedimentation zone due to the jetty could be filled with sand in advance, the smaller volume of sand might come from upstream zone. The study defines it as pre-beach nourishment. This study shows the design process of the pre-beach nourishment. The efficiency of the pre-beach nourishment will be investigated with the monitoring results of its real sea application.
Oh, Ki Won,Kim, Deog-Yoon,Lee, Yil-Seob,Kang, Moo Il Japanese Society of Bone and Mineral Metabolism ; 2012 Journal of bone and mineral metabolism Vol.30 No.3
<P>The Osteoporosis Patient Treatment Satisfaction Questionnaire (OPSAT-Q) is a psychometric measure of patient satisfaction with bisphosphonate treatment for osteoporosis. The study was a multicenter, nationwide, cross-sectional, patient-reported outcome study conducted to evaluate treatment satisfaction and quality of life using the OPSAT-Q in patients receiving oral bisphosphonate therapy. This study enrolled postmenopausal women from 43 hospitals and 112 clinics who had intermittently taken oral bisphosphonates for treatment of osteoporosis. 4,220 postmenopausal Korean women with a mean age of 65.3 years and a mean body mass index of 22.9 kg/m(2) participated in the study. All items within each subscale domain were more highly correlated with their hypothesized subscale domain relative to the other subscale domains, and all 16 items were significantly correlated with an overall composite satisfaction score (CSS). All scores showed acceptable internal consistency reliability (Cronbach's alpha >0.70, range 0.88-0.91). Comparisons of OPSAT-Q scores were made between selective subgroups of participants: monthly versus weekly administration, years of taking bisphosphonates, smoking habitus, acid-related medication and comorbid conditions. Mean OPSAT-Q subscale domains and CSS were higher for users of monthly bisphosphonates, with shorter duration, non-smokers, and non-users of acid-related medication. Mean OPSAT-Q subscale domain scores of side-effects were high for absence of comorbid conditions. The OPSAT-Q demonstrated acceptable measurement properties, including validity and reliability of subscale domains and CSS, in oriental women with postmenopausal osteoporosis. Postmenopausal women intermittently using oral bisphosphonate therapy reported increased satisfaction with monthly administration, with shorter duration, non-smokers, non-users of acid-related medication, and an absence of comorbid conditions.</P>