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Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zou, Shi-geng,Huang, Zhi-yi,Wang, Xiao-lan,Tao, Lin,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2
In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human $y^{+}$LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $y^{+}$LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat $y^{+}$LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest $y^{+}$LAT1 mRNA abundance and the lung had the lowest $y^{+}$LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine $y^{+}$LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.
Zhi-gang Tai,Yi-ren Zhu,Yi-bo Yuan,Jin Liu,Zhen-jie Li,Zhi-hua Liu,Kun-miao Wang 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.3
In this work, a highly sensitive method using a colorimetric probe coupled to dispersive liquid?liquid microextraction (DLLME) was developed for the quantitative determination of dopamine (DA) in serum. The DA in serum was concentrated by DLLME to increase the detection sensitivity and reduce the matrix effects. After the DLLME process, a colorimetric probe of silver triangular nanoparticles (AgTNPs) was used to detect DA, which was based on the plasma transformation of AgTNPs caused by strong interactions with melamine (MA). The results showed that DA could inhibit the aggregation of AgTNPs induced by MA, resulting in the recovery of the surface plasmon resonance (SPR) peak of AgTNPs. Thus, the DLLME method followed by colorimetric probe detection of DA can be achieved. The parameters affecting the proposed method were optimized, under the optimal conditions, a linear calibration curve was obtained over a concentration range of 5 to 250?nM with a recovery from 94.4 to 101.3%. The detection limit was 1.6 nM (at an S/N ratio of 3). The present method was successfully applied to determine DA in human serum.
Zhi, Ai-Min,Feng, Ding-Yuan,Zhou, Xiang-Yan,Zou, Shi-Geng,Huang, Zhi-Yi,Zuo, Jian-Jun,Ye, Hui,Zhang, Chang-Ming,Dong, Ze-Min,Liu, Zhun Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.8
Cationic amino acid transporter $b^{0,+}AT$ (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine $b^{0,+}AT$. Based on the sequence of porcine $b^{0,+}AT$ deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $b^{0,+}AT$ was isolated. The porcine $b^{0,+}AT$ cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse $b^{0,+}AT$, respectively. Real-time RT-PCR indicated porcine $b^{0,+}AT$ transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest $b^{0,+}AT$ mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest $b^{0,+}AT$ mRNA abundance while the colon had the lowest (p<0.05). The $b^{0,+}AT$ mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest $b^{0,+}AT$ expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).
Online Hop Timing Detection and Frequency Estimation of Multiple FH Signals
Zhi-Chao Sha,Zhang-Meng Liu,Zhi-Tao Huang,Yi-Yu Zhou 한국전자통신연구원 2013 ETRI Journal Vol.35 No.5
This paper addresses the problem of online hop timing detection and frequency estimation of multiple frequencyhopping (FH) signals with antenna arrays. The problem is deemed as a dynamic one, as no information about the hop timing, pattern, or rate is known in advance, and the hop rate may change during the observation time. The technique of particle filtering is introduced to solve this dynamic problem, and real-time frequency and direction of arrival estimates of the FH signals can be obtained directly, while the hop timing is detected online according to the temporal autoregressive moving average process. The problem of network sorting is also addressed in this paper. Numerical examples are carried out to show the performance of the proposed method.
Micro-scale PLA Fibrous Membranes for Adsorption of Cigarette Smoke
Yi-qing Gu,Zhe Wang,Ying Sun,Yi-Kai Wang,Zhi-juan Pan 한국섬유공학회 2018 Fibers and polymers Vol.19 No.3
Porous PLA fibrous membranes with different morphologies were successfully fabricated by varying the relative humidity. When the relative humidity was higher than 45 %, nanopores morphology appeared on the surface of PLA fibers. The micro-structures of PLA fibers and the packing densities and through-pore sizes of the PLA fibrous membranes were measured and found to greatly influence the smoke adsorption properties. The smoke adsorption was recorded in photos, and the process of how smoke adsorbed on the membranes was observed by SEM. The max removal efficiencies of nicotine, tar, water vapor, and carbon monoxide were 40.0 %, 36.9 %, 5.8 % and 13.6 % measured by a cigarette-smoking machine, and sensory evaluation was conducted to assess the practical application of fibrous membranes for smoke adsorption.
Seismic performance and optimal design of framed underground structures with lead-rubber bearings
Zhi-Yi Chen,Hu Zhao,Meng-Lin Lou 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.58 No.2
Lead-rubber bearings (LRBs) have been used worldwide in seismic design of buildings and bridges owing to their stable mechanical properties and good isolation effect. We have investigated the effectiveness of LRBs in framed underground structures on controlling structural seismic responses. Nonlinear dynamic time history analyses were carried out on the well-documented Daikai Station, which collapsed during the 1995 Hyogoken-Nanbu earthquake. Influences of strength ratio (ratio of yield strength of LRBs to yield strength of central column) and shear modulus of rubber on structural seismic responses were studied. As a displacement-based passive energy dissipation device, LRBs reduce dynamic internal forces of framed underground structures and improve their seismic performance. An optimal range of strength ratios was proposed for the case presented. Within this range, LRBs can dissipate maximum input earthquake energy. The maximum shear and moment of the central column can achieve more than 50% reduction, whereas the maximum shear displacement of LRBs is acceptable.
Zhi-Zhun Mo,Zhi-Xiu Lin,ZiRen Su,Lin Zheng,Hui-Lin Li,JianHui Xie,Yan-Fang Xian,Tie-Gang Yi,Shui-Qing Huang,Jian-Ping Chen 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.9
Angelica sinensis (AS, Danggui in Chinese) is an important herbal component of various traditional formulae for the management of asthenia and its tonic effects. Although AS has been shown to ameliorate cognitive damage and nerve toxicity in D-galactose (D-gal)-elicited senescent mice brain, its effects on liver and kidney injury have not yet been explored. In this work, mice were subjected to hypodermic injection with D-gal (200 mg/kg) and orally gavaged with AS (20, 40, or 80 mg/kg) once a day for 8 successive weeks. Results revealed that AS significantly improved liver and kidney function as assessed by organ index and functional parameters. In addition, AS pretreatment effectively ameliorated the histological deterioration. AS attenuated the MDA level and markedly enhanced the activities and gene expressions of antioxidative enzymes, namely Cu, Zn-SOD, CAT, and GPx. Furthermore, AS markedly inhibited the D-gal-mediated increment of expressions of inflammatory cytokines iNOS, COX-2, IκBα, p-IκBα, and p65 and promoted the IκBα expression level in both hepatic and renal tissues. In sum, AS pretreatment could effectively guard the liver and kidney of mice from D-gal-induced injury, and the underlying mechanism was deemed to be intimately related to attenuating oxidative response and inflammatory stress.
Testing for diffusion in a discretely observed semimartingale
Bing-Yi Jing, Zhi Liu,Xin-Bing Kong 한국통계학회 2010 Journal of the Korean Statistical Society Vol.39 No.3
Using pth power variation, we propose a nonparametric method to test whether a continuous diffusion is present in a semimartingale. Under the discrete observation of high frequency data, the test statistic converges to a known constant if a diffusion is present,otherwise it tends to another number. The test procedure is valid for all Itô semimartingales and does not depend on the law of a process or the coefficients of a semimartingale. We also implement the test on simulation and real asset return data.
( Xiu Zhi Gao ),( Hui Liu ),( Xin Xin Yi ),( Yi Qian Liu ),( Xiao Dong Wang ),( Wen Sheng Xu ),( Qi Gen Tong ),( Zong Jun Cui ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.12
Traditional soybean paste from Shandong Liangshan and Tianyuan Jiangyuan commercial soybean paste were chosen for analysis and comparison of their bacterial and fungal dynamics using denaturing gel gradient electrophoresis and 16S rRNA gene clone libraries. The bacterial diversity results showed that more than 20 types of bacteria were present in traditional Shandong soybean paste during its fermentation process, whereas only six types of bacteria were present in the commercial soybean paste. The predominant bacteria in the Shandong soybean paste were most closely related to Leuconostoc spp., an uncultured bacterium, Lactococcus lactis, Bacillus licheniformis, Bacillus spp., and Citrobacter freundii. The predominant bacteria in the Tianyuan Jiangyuan soybean paste were most closely related to an uncultured bacterium, Bacillus licheniformis, and an uncultured Leuconostoc spp. The fungal diversity results showed that 10 types of fungi were present in the Shandong soybean paste during the fermentation process, with the predominant fungi being most closely related to Geotrichum spp., an uncultured fungal clone, Aspergillus oryzae, and yeast species. The predominant fungus in the commercial soybean paste was Aspergillus oryzae.