Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

Comparison of stomach microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing

Wen‑kui Sun,Yanyue Li,Chi Cheng,Yi‑hui Chen,Kai Zeng,Xiaohui Chen,Yiren Gu,Rui Liu,Xuebin Lv,Rong Gao 한국유전학회 2018 Genes & Genomics Vol.40 No.9

MiRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in different physiological processes, yet little is known about the stomach microRNAome (miRNAome) of the Tibetan pig. The objective of this experiment was to investigate differentially expressed stomach miRNAs participating in digestion. Firstly, we isolated total RNA by Trizol reagent from three Tibetan and three Yorkshire purebred pigs stomach samples at 90-day-old. Secondly, a comprehensive analysis of Tibetan and Yorkshire pig stomach miRNAomes was performed by small RNA sequencing in the Illumina HiSeq 2000 system. Finally, SYBR Green Real-time RT-PCR was performed to validate the differentially expressed miRNAs. We identified 318 unique miRNAs, 260 were co-expressed in both libraries, 17 and 31 miRNAs were specifically expressed in Tibetan and Yorkshire pigs respectively. Fifty six differentially expressed miRNAs were identified by the identifying differentially expressed genes 6 (IDEG6). Kyoto encyclopedia of genes and genomes analysis revealed that some of the differentially expressed miRNAs were associated with protein and fat digestion. Two differentially expressed miRNAs (miR-214-3p and ssc-un39) participating in the digestion of lipid were identified. Additionally, qRT-PCR results suggested that a higher expression of miR-214-3p in the Tibetan pig stomach could lead to relatively lower expression of calcium-dependent phospholipase A2, which is an enzyme important for the digestion of glycerol phospholipid. This study has delineated the different stomach miRNAs expression patterns of Tibetan and Yorkshire pigs, which would help explain the regulatory mechanisms of miRNAs in digestion of Tibetan pigs, and contribute to utilize a the unique digestion merits of Tibetan pig in future porcine hybridization breeding.

Research on Current Execution of Customer Support Knowledge Management System of Medical Appliances Industry

( Yi Chan Chung ),( Chih Hung Tsai ),( Shiaw Wen Tien ),( Lin Yi Lin ) 한국품질경영학회 2007 The Asian Journal on Quality Vol.8 No.3

Simulation and Comparison of the Lighting Efficiency for Household Illumination with LEDs and Fluorescent Lamps

Wen-Shing Sun,Chuen-Lin Tien,Jui-Wen Pan,Tsung-Hsun Yang,Chih-Hsuan Tsuei,Yi-Han Huang 한국광학회 2013 Current Optics and Photonics Vol.17 No.5

The design of the LEDs lighting in general household illumination was proposed and compared with the fluorescent lighting in this study. Using the LED as a light source would promote energy saving lighting for household illumination purposes. We used the LightTools and DIALux software to design and simulate different standards of illuminance, different correlated color temperatures and different color rendering indices for household environments. The power consumption and efficiency of traditional illuminated light sources and an LED light source with the same standard of illuminance for lighting the household environment were analyzed and compared with each other. Finally, our results show the advantages of using white-light LEDs for lighting and household illumination.

중국 윈난성 뎬츠(滇池)보호에서의 공중참여에 관한 연구 : 자원동원이론의 시각으로

Yi-Hua Wen,Yue Sun,Ming Liu 한국환경사회학회 2019 환경사회학연구 ECO Vol.23 No.2

This research takes the private environment organization--Green Kunming as an example, to explore the growth of resources by mobilization. The study includes a public participation activity--Protecting Lake Denz, the largest lake in Kunming, Yunnan Province. To ensure this organization survive and thrive, we must have sufficient resources and are able to mobilize them. This study focuses on the development of Green Kunming ancestry conservation activities based on institutional resources, financial resources, human resources and social resources. These resources are organically integrated with each other and are characterized by collective actions. The survey results show that Green Kunming is limited by institutional resources, but this is also a prerequisite for obtaining legitimacy and obtaining new resources. Financial resources are mainly funded by private fund meetings. and these funds are a major participatory survival method including projects. In mobilizing human resources, the members are mainly volunteers. Doing this will ensure the sustainability of group development, promote the formation of social capital, and fully mobilize new media as a social resource. This study aims to determine the growth of citizens in our society, and hope to use it as an indicator to measure the degree of the political structure openness to the Chinese public participation and the degree of democratization in the public sphere. 본 연구는 중국 윈난성 쿤밍시에서 가장 큰 호수인 뎬츠(滇池)에 대한 보호를 비롯한 공중의 참여활동 중에서 민간 환경단체-녹색곤명을 사례로, 자원동원론의 시각으로 참여과정에 자원을 어떻게 동원하면서 성장하였는가를 살펴본다. 단체가 생존하고 성장하려면 다양한 자원을 소유함과 아울러 자원을 충분히 동원하여야 한다. 이런 자원들은 또 서로 유기적으로 결합되면서 단체활동 전개의 특성을 가진다. 본고에서는 자원을 동원하기 위한 전제조건, 재정적 자원, 인적 자원, 사회적 자원을 토대로 뎬츠보호의 주요한 행위주체‒녹색곤명의 뎬츠보호 활동의 전개에 대한 분석이 중심을 이룬다. 연구결과 녹색곤명은 합법적인 지위를 취득하여 새로운 자원을 얻을 수 있는 정치적 기회구조를 확충하고, 재정적 자원은 주로 민간 기금회의 자금 지원을 통해 얻는데 이러한 자금조달은 프로젝트를 비롯한 참여방식이 된다. 인적 자원의 동원을 볼때 구성원은 주로 자원봉사자들인데 이러한 특성은 인력동원이 용이하고 자발적으로 활동할 수 있으며 단체 발전의 지속성을 보장하며, 사회자본의 형성을촉진할 수 있다. 사회적 자원으로는 주로 뉴미디어를 활용했는데 전파속도가빠르고 전파범위가 넓어서 공론장의 형성을 촉진하였다. 이러한 현상을 통해중국에서 공중참여에 대한 제도적 보장은 이미 갖춰져 있고, 정치적 기회 구조는 질적으로 변화하고 있으며, 정부의 집권이념의 변화가 시민사회의 성장을추진하고 있다는 결론을 얻을 수 있었다.

Fabrication of Photopolymer Hierarchical Micronanostructures by Coupling Electrospinning and Photolithography for SERS Substrates

Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3

Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.

An NIR-Absorbing Nanoparticle System Loaded with TLR-7/8 Ligand for Combinational Photothermal Immunotherapy

Wen-Yu PAN,Po-Ming CHEN,Yi-Hsuan TSAI,Hsing-Wen SUNG 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

The Influence of Reconstruction Algorithm and Heart Rate on Coronary Artery Image Quality and Stenosis Detection at 64-Detector Cardiac CT

Yi-Ting Wang,Chung-Yi Yang,Jong-Kai Hsiao,Hon-Man Liu,Wen-Jen Lee,Yun Shen 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.3

Objective: We wanted to evaluate the impact of two reconstruction algorithms (halfscan and multisector) on the image quality and the accuracy of measuring the severity of coronary stenoses by using a pulsating cardiac phantom with different heart rates (HRs). Materials and Methods: Simulated coronary arteries with different stenotic severities (25, 50, 75%) and different luminal diameters (3, 4, 5 mm) were scanned with a fixed pitch of 0.16 and a 0.35 second gantry rotation time on a 64-slice multidetector CT. Both reconstruction algorithms (halfscan and multisector) were applied to HRs of 40-120 beats per minute (bpm) at 10 bpm intervals. Three experienced radiologists visually assessed the image quality and they manually measured the stenotic severity. Results: Fewer measurement errors occurred with multisector reconstruction (p = 0.05), a slower HR (p < 0.001) and a larger luminal diameter (p = 0.014); measurement errors were not related with the observers or the stenotic severity. There was no significant difference in measurements as for the reconstruction algorithms below an HR of 70 bpm. More nonassessable segments were visualized with halfscan reconstruction (p = 0.004) and higher HRs (p < 0.001). Halfscan reconstruction had better quality scores when the HR was below 60 bpm, while multisector reconstruction had better quality scores when the HR was above 90 bpm. For the HRs between 60 and 90 bpm, both reconstruction modes had similar quality scores. With excluding the nonassessable segments, both reconstruction algorithms achieved a similar mean measured stenotic severity and similar standard deviations. Conclusion: At a higher HR (above 90 bpm), multisector reconstruction had better temporal resolution, fewer nonassessable segments, better quality scores and better accuracy of measuring the stenotic severity in this phantom study. Objective: We wanted to evaluate the impact of two reconstruction algorithms (halfscan and multisector) on the image quality and the accuracy of measuring the severity of coronary stenoses by using a pulsating cardiac phantom with different heart rates (HRs). Materials and Methods: Simulated coronary arteries with different stenotic severities (25, 50, 75%) and different luminal diameters (3, 4, 5 mm) were scanned with a fixed pitch of 0.16 and a 0.35 second gantry rotation time on a 64-slice multidetector CT. Both reconstruction algorithms (halfscan and multisector) were applied to HRs of 40-120 beats per minute (bpm) at 10 bpm intervals. Three experienced radiologists visually assessed the image quality and they manually measured the stenotic severity. Results: Fewer measurement errors occurred with multisector reconstruction (p = 0.05), a slower HR (p < 0.001) and a larger luminal diameter (p = 0.014); measurement errors were not related with the observers or the stenotic severity. There was no significant difference in measurements as for the reconstruction algorithms below an HR of 70 bpm. More nonassessable segments were visualized with halfscan reconstruction (p = 0.004) and higher HRs (p < 0.001). Halfscan reconstruction had better quality scores when the HR was below 60 bpm, while multisector reconstruction had better quality scores when the HR was above 90 bpm. For the HRs between 60 and 90 bpm, both reconstruction modes had similar quality scores. With excluding the nonassessable segments, both reconstruction algorithms achieved a similar mean measured stenotic severity and similar standard deviations. Conclusion: At a higher HR (above 90 bpm), multisector reconstruction had better temporal resolution, fewer nonassessable segments, better quality scores and better accuracy of measuring the stenotic severity in this phantom study.

Research on a Multi-Objective Control Strategy for Current-source PWM Rectifiers under Unbalanced and Harmonic Grid Voltage Conditions

Yi-Wen Geng,Hai-Wei Liu,Ren-Xiong Deng,Fang-Fang Tian,Hao-Feng Bai,Kai Wang 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1

Unbalanced and distorted grid voltages cause the grid side current of a current source PWM rectifier to be heavily distorted. They can also cause the DC-link current to fluctuate with a huge amplitude. In order to enhance the performance of a current-source PWM rectifier under unbalanced and harmonic grid voltage conditions, a mathematical model of a current-source PWM rectifier is established and a flexible multi-objective control strategy is proposed to control the DC-link current and grid-current. The fundamental positive/negative sequence, 5th and 7th order harmonic components of the grid voltage are first separated with the proposed control strategy. The grid current reference are optimized based on three objectives: 1) sinusoidal and symmetrical grid current, 2) sinusoidal grid current and elimination of the DC-current 2nd order fluctuations, and 3) elimination of the DC-current 2nd and 6th order fluctuations. To avoid separation of the grid current components, a multi-frequency proportional-resonant controller is applied to control the fundamental positive/negative sequence, 5th and 7th order harmonic current. Finally, experimental results verify the effectiveness of proposed control strategy.

PF2405, standardized fraction of Scutellaria baicalensis, ameliorates colitis in vitro and in vivo

Wen-Yi Jiang,서검석,김윤철,손동환,이성희 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Standardized extraction procedures for herb areas important as their authentication to maintain theirquality and ensure their safe use. We had prepared astandardized and purified Scutellaria baicalensis Georgiextract, PF2405, which was enriched with three majorcomponents, baicalein, oroxylin A and wogonin. In thepresent study, we investigated the potential anti-inflammatoryeffects of PF2405 in vitro and in two differentexperimental animal models of inflammatory bowel disease. Effect of PF2405 studied in tumor necrosis factor(TNF)-a-induced HT-29 cells in vitro. In vivo experimentalcolitis models were induced by administration oftrinitrobenzene sulfonic acid (TNBS) or dextran sulfatesodium (DSS). PF2405 (50 lg/ml) decreased TNF-ainducedcyclooxygenase (COX)-2 expressions throughinhibition of phosphorylation of c-Jun N-terminal kinasesand p38 mitogen-activated protein kinase in HT-29 cells. Combination of baicalein (20 lg/ml), oroxylin A (8 lg/ml),and wogonin (2 lg/ml) markedly inhibits TNF-a-inducedCOX-2 expression when compared with individual components. PF2405 (25 mg/kg b.w.) treatment significantlyreduced histopathological severity; suppressed expression ofCOX-2, TNF-a, and interleukin-1b in TNBS-induced mice. Moreover, PF2405 (25 mg/kg b.w.) has both potent preventiveand therapeutic activities in DSS-induced colitis. Collectively, PF2405 shows prominent anti-inflammatoryeffect that can be used as a new therapeutic approach forintestinal inflammatory disorders.