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Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1
The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.
The Development and Current State of Inclusive Education at Universities in Taiwan
Hsin Yi Wang 아시아지적장애인연맹 산하 아시아저널센터 2019 Asia-Pacific Journal of Intellectual Disabilities Vol.6 No.2
The purpose of this study is to investigate the development of current state of inclusive education at universities in Tiwan. In Tiwan, inclusive education is for all students who are disabled/non-disabled. We believe that inclusive education ensure the educational right of all students regardless of the type and degree of individual difference. However, challenges remain in the inclination of teaching staff to modify their teaching materials, as this is currently not in line with the United Nations Convention on the Rights of Persons with Disabilities (CRPD) obligations. Furthermore, higher education is not part of compulsory education, a fact which can be a point of debate and represent a challenge to implementing inclusive education in this setting. In conclusion, students with developmental disabilities who cannot comprehend the theoretical or academic subjects being studied in higher education may be better served by appropriate transfers or bridge courses aimed to deliver career-oriented knowledge and training.
王一川 中國語文硏究會 2003 中國語文論叢 Vol.24 No.-
본 논문은 세계화 맥락 속에서 중국의 문예현황을 논술하고 있다. 세계화는 단순히 세계의 일체화를 가리키는 것이 아니라, 세계의 일체화, 세계체계론과 사회전환론 등 세 종류의 의의를 함축하고 있어 세계화에 대한 단편적인 오해를 바로 잡을 필요가 있다. 세계화는 실제적으로 현대인의 전세계적 공생과 상호적인 체험을 가리키는 것으로 16세기 이래 세계 각 민족간의 상호의존과 삼투가 나날이 긴밀해지는 생활방식이며, 특정지역의 생존체험과 멀리 떨어진 지역의 생존체험이 이러 저러한 연관을 맺는 것을 의미한다. 이러한 세계화의 맥락 속에서 예술을 고찰하기 위해서는 생산과 소비영역에서의 예술의 종족, 매개, 과학기술, 금융과 이데올로기 등 다층적인 상황을 고려해야 하며, 예술의 주류문화, 고급문화, 대중문화와 민간문화 등 다양한 형태간의 다원적인 상호삼투상황, 그리고 새로운 다원화된 변화생성의 양태를 살펴보아야 한다.
半夏瀉心湯이 CCl4 로 유도된 간중독 흰쥐에 미치는 영향
朱旺錫,朴賢俊,尹炳局,鄭成伊,朴宣東 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2
The purpose of this study is to observe the effect of Banhasasimtang on serum reaction in CCI₄ treated rats. In this study the experimental rats divided five group(Normal, Control, Sample A, Sample B and Sample C group) Under the same condition Normal group was fed basal diet and water, Control group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and basal diet for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄0.5㎖/㎏) and fed the Bahasasimtang extract(10㎖/㎏) for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks, Sample B group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks and injected carbon tetrachloride(CCI₄ 0.5㎖/㎏), Sample C group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks. The change of GOT, GPT, γ-GPT, ALP, LDH activity and Bilirubin level in blood serum. The obtained results are summarized as follows : 1. In the change of GOT GPT contents, as compared with control group, sample group was significantly decreased. 2. In the change of γ-GPT contents, as compared wth control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDP contents, as compared with control group, sample group was significantly decreased. 5. In the change of Bilirubin contents, as compared with control group, sample group was significantly decreased.
Studies on Layered Modulation for SVC Signals in DVB-S2 System
Yi Wang,Seung-Chul Kim,Kye-San Lee,Won Sohn 한국방송·미디어공학회 2008 한국방송공학회 학술발표대회 논문집 Vol.2008 No.-
The paper describes a Layered Modulation using the SVC signals and studies the properties of the modulation with respect to several parameters by the computer simulation. The SVC signals will include a base layer signal and an enhancement signal, and the base layer signal is the more important one in its channel robustness. The parameters will include a carrier frequency, a bandwidth, power level, modulation type and code rate. We analyze the demodulating and decoding process of the Layered Modulation system through several scatter plots. And then we discuss the affect of the layer signal power difference to the BER performance, which also proves the base layer signal is more important than the enhancement layer signal.
Yi, Yi,Qiao, Dairong,Bai, Linhan,Xu, Hui,Li, Ya,Wang, Xiaolin,Cao, Yi The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.2
5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.
Influence of Atmospheric Turbulence Channel on a Ghost-imaging Transmission System
Wang, Kaimin,Wang, Zhaorui,Zhang, Leihong,Kang, Yi,Ye, Hualong,Hu, Jiafeng,Xu, Jiaming Optical Society of Korea 2020 Current Optics and Photonics Vol.4 No.1
We research a system of compressed-sensing computational ghost imaging (CSCGI) based on the intensity fluctuation brought by turbulence. In this system, we used the gamma-gamma intensity-fluctuation model, which is commonly used in transmission systems, to simulate the CSCGI system. By setting proper values of the parameters such as transmission distance, refractive-index structure parameter, and sampling rates, the peak signal-to-noise ratio (PSNR) performance and bit-error rate (BER) performance are obtained to evaluate the imaging quality, which provides a theoretical model to further research the ghost-imaging algorithm.
Tazarotene-Induced Gene 1 Interacts with DNAJC8 and Regulates Glycolysis in Cervical Cancer Cells
Wang, Chun-Hua,Shyu, Rong-Yaun,Wu, Chang-Chieh,Chen, Mao-Liang,Lee, Ming-Cheng,Lin, Yi-Yin,Wang, Lu-Kai,Jiang, Shun-Yuan,Tsai, Fu-Ming Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6
The tazarotene-induced gene 1 (TIG1) protein is a retinoidinducible growth regulator and is considered a tumor suppressor. Here, we show that DnaJ heat shock protein family member C8 (DNAJC8) is a TIG1 target that regulates glycolysis. Ectopic DNAJC8 expression induced the translocation of pyruvate kinase M2 (PKM2) into the nucleus, subsequently inducing glucose transporter 1 (GLUT1) expression to promote glucose uptake. Silencing either DNAJC8 or PKM2 alleviated the upregulation of GLUT1 expression and glucose uptake induced by ectopic DNAJC8 expression. TIG1 interacted with DNAJC8 in the cytosol, and this interaction completely blocked DNAJC8-mediated PKM2 translocation and inhibited glucose uptake. Furthermore, increased glycose uptake was observed in cells in which TIG1 was silenced. In conclusion, TIG1 acts as a pivotal repressor of DNAJC8 to enhance glucose uptake by partially regulating PKM2 translocation.
Energy Efficient Feedback Design for Coordinated Multi-Point Transmission in Downlink
Yi Wang,Yanjun Hu,Yiqing Zhou 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.6
Coordinated multi-point (CoMP) has been raised to increase the average cell throughput and the cell-edge user throughput. However, the energy consumption of mobile stations (MSs) is a key problem restricting the wide application of CoMP systems. Considering the energy consumption of feeding back channel state information (CSI) by MSs and the influence of CSI accuracy on the throughput of system, it is proposed to measure the relationship between feedback energy consumption and throughput with energy efficiency feedback utility (EEFU) function which can adapt to different application scenes by adjusting utilization coefficient. The feedback utilization of two precoders in CoMP systems is analyzed respectively, including the optimization allocation of feedback bits with utility function. The complete EEFU optimized expression and the optimizing flow path satisfying the actual application demands of CoMP systems are then derived. The simulation results illustrate the practicability and necessity of the EEFU function and verify the performance of EEFU with different precoders.
Modeling and Simulation of Discharging Characteristics of External Melt Ice-on Coil Storage System
Wang Yi,Wei Dong 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.2
In case of energy storage air conditioning system, due to load fluctuation, the traditional control methods can't obtain good effect, the model predictive control method can realize the optimization control system, and can overcome the influence of load fluctuation. In order to realize the predictive control, need to study model of air conditioning system. Ice storage air-conditioning system, by analyzing the mechanism of ice tank, ice melting system outside the tank model is established. First, analyzed the physical structure of ice bath, determine the parameters of ice bath, put forward the control as well as the output of the ice tank. Good model will be built using TRNSYS and MATLAB joint simulation, and the model simulation results and the widely used now BAC coil and TSQINGHUA TONGFANG ice coil characteristic has carried on the contrast, determine the tank model is built by ice in the most good ice tank was simulated under the condition of outlet temperature, and focused on the top 20% of the ice melted and the error of 20%, after put forward the reasonable solution, the interpretation of the ice tank established ice model has practical significance.