Hypolipidemic and Antioxidative Effects of Glossogyne tenuifolia in Hamsters Fed an Atherogenic Diet

Yi-Ning Lee,Guoo-Shyng Wang Hsu,Wan-Teng Lin,Yi-Fa Lu 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.5

Glossogyne tenuifolia (GT) Cassini is a special herbal tea in the Penghu Islands, Taiwan, and has a long history of being used as an antipyretic, detoxifying, and anti-inflammatory remedy in folk medicine among local residents. The aim of this study was to investigate the effect of hot water extracts from GT on oxidative stress and lipid metabolism in animals. Five- to 6-week-old male Syrian hamsters were divided into four groups (n = 14) for different treatments, that is: control group (C), high-fat/cholesterol (HF) group, HF diet containing 0.5% (GT0.5) and 1.5% (GT1.5) GT extracts for 4 weeks. Hamsters fed with 0.5% GT powder as well as 1.5% GT powder exhibited reduced serum total cholesterol (TC), conjugated diene of low-density lipoprotein (LDL), and increased serum antioxidant capacity, but 1.5% GT powder was more potent at loweringserum LDL cholesterol and thiobarbituric acid reactive substance concentrations than 0.5% GT. GT extracts significantly lowered liver triacylglycerol (TG) concentration by diminishing activities of fatty acid synthase (FAS) and glucose-6-phosphate dehydrogenase (G-6-PDH). In addition, fecal excretion of cholesterol and bile acids were increased in GT extract groups. In conclusion, GT extracts increase the antioxidative capacity, decrease serum TC, inhibit the activities of FAS and G-6-PDH, and further reduce liver TG accumulation in hamster fed on atherogenic diets.

Speech-driven talking face using embedded confusable system for real time mobile multimedia

Shih, Po-Yi,Paul, Anand,Wang, Jhing-Fa,Chen, Yi-Hung Springer-Verlag 2014 MULTIMEDIA TOOLS AND APPLICATIONS Vol.73 No.1

Synthesis of Thermoresponsive Hyperbranched Polyamidoamine and the Molecular Weight, pH, and Anion Sensitive Thermoresponsive Properties Thereof

Yi Liu,Yu Chen,Xun-Yong Liu,Hua-Ji Liu,Fa Cheng 한국고분자학회 2012 Macromolecular Research Vol.20 No.6

Hyperbranched polyamidoamine (HPAMAM) polymers, chemically analogous to the commercially available PAMAM dendrimer, were modified with isobutyric anhydride to result in isobutyramide (IBAm) terminated HPAMAMs (HPAMAM-IBAm). The aqueous solutions of HPAMAM-IBAm polymers had the lower critical solution temperature (LCST). The lower molecular-weight HPAMAM-IBAm exhibited higher LCST and the LCST difference was around 18 oC for one pseudo-generation variation. Further, the hyperbranched thermoresponsive polymers exhibited much lower LCSTs than the corresponding dendrimers with similar molecular weight. The LCST of HPAMAM-IBAm was pH sensitive. At pH below 10, the LCST increased significantly upon decreasing the pH,whereas, at pH above 10, the LCST decreased slowly with an increasing pH value. Nine sodium salts were used to measure the anion effect on the LCST of HPAMAM-IBAm. It was found that the LCST could also be modulated up or down in a broad range by simply adding a small amount of different kinds of inorganic anions. The specific ranking of inorganic anions in salting-out HPAMAM4-IBAm polymer was in accordance with the well-known Hofmeister series.

Mechanical behavior of outer square inner circular concrete-filled dual steel tubular stub columns

Fa-Xing Ding,Wenjun Wang,Xue-mei Liu,Liping Wang,Yi Sun 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.3

The mechanical behavior of the outer square inner circular concrete-filled dual steel tubular (SCCFT) stub columns under axial compression is investigated by means of experimental research, numerical analysis and theoretical investigation. Parameters such as diameter ratio, concrete strength and steel ratio were discussed to identify their influence on the mechanical properties of SCCFT short columns on the basis of the experimental investigation of seven SCCFT short columns. By establishing a finite element model, nonlinear analysis was performed to discuss the longitudinal and transverse stress of the dual steel tubes. The longitudinal stress characteristics of the core and sandwich concrete were also analyzed. Furthermore, the failure sequence was illustrated and the reasonable cross-section composition of SCCFT stub column was proposed. A formula to predict the axial load capacity of SCCFT stub column was advanced and verified by the results from experiment and the finite element.

Confinement coefficient of concrete-filled square stainless steel tubular stub columns

Fa-Xing Ding,Yi-xiang Yin,Liping Wang,Yujie Yu,Liang Luo,Zhi-wu Yu 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.30 No.4

The objective of this paper is to investigate the confinement coefficient of concrete-filled square stainless steel tubular (CFSSST) stub columns under axial loading. A fine finite 3D solid element model was established, which utilized a constitutive model of stainless steel considering the strain-hardening characteristics and a triaxial plastic-damage constitutive model of concrete with features of the parameter certainty under axial compression. The finite element analysis results revealed that the increased ultimate bearing capacity of CFSSST stub columns compared with their carbon steel counterparts was mainly due to that the composite action of CFSSST stub columns is stronger than that of carbon steel counterparts. A further parametric study was carried out based on the verified model, and it was found that the stress contribution of the stainless steel tube is higher than the carbon steel tube. The stress nephogram was simplified reasonably in accordance with the limit state of core concrete and a theoretical formula was proposed to estimate the ultimate bearing capacity of square CFSSST stub columns using superposition method. The predicted results showed satisfactory agreement with both the experimental and FE results. Finally, the comparisons of the experimental and predicted results using the proposed formula and the existing codes were illustrated.

Low temperature oxidation characteristics analysis of ultra-heavy oil by thermal methods

Yi-Bo Li,Ya-Fei Chen,Wan-Fen Pu,Hong Dong,Hao Gao,Fa-yang Jin,Bing Wei 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-

To study the oxidation behavior of heavy oils, low temperature oxidation experiments under differentialtemperature and pressure conditions were carried out for both heavy oils (ordinary heavy and ultraheavyoils) with thermogravimetry (TG–DTG)/differential scanning calorimeter (DSC) analyses. And theresults indicated that the differences of physical properties and oxidation reactions for the heavy oilsascribe to the SARA compositions, H/C ratio and specific oxidation path. Owing to the asphaltenescontent, the ultra-heavy oil after the oxidation has more obvious coke deposition with lower activationenergy demand to make it possible for the in-situ combustion without combustion adjuvants.

Primary Pericardial Angiosarcoma: an Insidious and Aggressive Malignant Tumor

Guan-Yi Li,Fa-Po Chung 대한심장학회 2020 Korean Circulation Journal Vol.50 No.10

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Optimization of Medium Composition for Improving Biomass Production of Lactobacillus plantarum Pi06 Using the Taguchi Array Design and the Box-Behnken Method

Chin-Fa Hwang,Jen-Han Chang,Jer-Yiing Houng,Cheng-Chih Tsai,Chien-Ku Lin,Hau-Yang Tsen 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4

An immune-enhancing strain, Lactobacillus plantarum Pi06, isolated from a healthy infant was used for biomass production following optimization of the medium in shake-flask culture. Preliminary studies showed that commercial MRS medium and cultivation under static conditions generated higher biomass production than four other tested media with or without a shaking condition. The selected medium composition, consisting of glucose,yeast extract, soy peptone, ammonium citrate, and corn steep liquor, was further optimized using a systematic method that integrated the Taguchi array design and the Box–Behnken method. The response effects of these factors were first investigated using Taguchi design under an L16 (45) array. The suggested medium composition,derived from Statistica 7.1 using the Taguchi design, was applied to cultivate cells and a biomass of 7.16 g dry cell weight (DCW)/L was obtained. Response surface methodology based on the Box–Behnken method for the three response variables of glucose, yeast extract, and corn steep liquor was then used to further increase the biomass level to 8.94 g DCW/L. The resulting optimum medium consisted of 35 g/L glucose, 35 g/L yeast extract, and 40 mL/L corn steep liquor. Compared with the initial medium, the biomass yield was improved from 4.31 to 8.94 g DCW/L, an enhancement of approximately 107%.

Cell Surface Sialylation Mediates the Binding of Enterovirus 71 to Host Cells

Pei-Yi Su,Yueh-Tung Liu,Chuan-Fa Chang 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Enterovirus 71 (EV71) is a major causative agent of hand-foot-and-mouth disease (HFMD). The clinical severity of EV71central nerve system (CNS) infection is mostly in children less than 5 years-old. Recently, the twoglycosylated membrane proteins SCARB2 and PSGL-1 have been identifiedas the cellular and functional receptors of EV71.But the role of glycosylation in EV71 infection remainsunclear. In this study, we demonstrated thatthe attachment of EV71 to two susceptible cell lines werediminished after the removal of cell surface sialic acids. Pre-incubatecells with sialic acid specific lectins, MAA and SNA,reducedthe binding of EV71. Additionally, we found that SCARB2 was a sialylated glycoprotein,andthe interaction of SCARB2and EV71was decreased after desialylation. Based on these results, we concluded that the attachment of EV71 to cell surface was assisted bysialic acids.Cell surface sialylation should be a key regulator that facilitates the binding and infection of EV71.

The dopamine D1–D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury

Bao Yi-Ni,Dai Wen-Ling,Fan Ji-Fa,Ma Bin,Li Shan-Shan,Zhao Wan-Li,Yu Bo-Yang,Liu Ji-Hua 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Dopamine D1 receptor (D1DR) and D2 receptor (D2DR) are closely associated with pain modulation, but their exact effects on neuropathic pain and the underlying mechanisms remain to be identified. Our research revealed that intrathecal administration of D1DR and D2DR antagonists inhibited D1–D2DR complex formation and ameliorated mechanical and thermal hypersensitivity in chronic constriction injury (CCI) rats. The D1–D2DR complex was formed in the rat spinal cord, and the antinociceptive effects of D1DR and D2DR antagonists could be reversed by D1DR, D2DR, and D1–D2DR agonists. Gαq, PLC, and IP3 inhibitors also alleviated CCI-induced neuropathic pain. D1DR, D2DR, and D1–D2DR complex agonists all increased the intracellular calcium concentration in primary cultured spinal neurons, and this increase could be reversed by D1DR, D2DR antagonists and Gαq, IP3, PLC inhibitors. D1DR and D2DR antagonists significantly reduced the expression of p-PKC γ, p-CaMKII, p-CREB, and p-MAPKs. Levo -corydalmine ( l -CDL), a monomeric compound in Corydalis yanhusuo W.T. Wang, was found to obviously suppress the formation of the spinal D1–D2DR complex to alleviate neuropathic pain in CCI rats and to decrease the intracellular calcium concentration in spinal neurons. l- CDL-induced inhibition of p-PKC γ, p-MAPKs, p-CREB, and p-CaMKII was also reversed by D1DR, D2DR, and D1–D2DR complex agonists. In conclusion, these results indicate that D1DR and D2DR form a complex and in turn couple with the Gαq protein to increase neuronal excitability via PKC γ, CaMKII, MAPK, and CREB signaling in the spinal cords of CCI rats; thus, they may serve as potential drug targets for neuropathic pain therapy.