Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

Terfenadine과 Ketoconazole 병용투여에 의해 유발된 Torsade de Pointes1예

오석규,국향,임수빈,정진원,박양규,박옥규 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.3

양측 쇄골하동맥 및 양측 신동맥 협착을 동반한 Takayasu 동맥염 1예

임선호,임수빈,오석규,이재홍,정진원,박양규,박옥규,소병준,노병석 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

Two Newly Recorded Species of the Genus Ypsolopha Latreille (Lepidoptera: Ypsolophidae) from Korea

나솔문,안능호,변봉규,배양섭,Na, Sol-Moon,Ahn, Neung-Ho,Byun, Bong-Kyu,Bae, Yang-Seop Korean Society of Applied Entomology 2016 한국응용곤충학회지 Vol.55 No.1

Ypsolopha속 (나비목:Ypsolophidae)의 2종, Ypsolopha vittella (Linnaeus, 1758)와 Ypsolopha nigrofasciata (Yang, 1977)를 우리나라에서는 처음으로 보고한다. 이 두 종의 진단형질, 기재, 분포, 기주식물, 성충사진 및 암컷 생식기 사진을 제시하였다. Two species of the genus Ypsolopha Latreille are reported for the first time from Korea: Ypsolopha vittella (Linnaeus) and Y. nigrofasciata Yang. The diagnosis, description, distribution, host plants, adult photographs, and pictures of female genitalia are provided.

한국성인에서의 Tweed Triangle과 Soft-Tissue에 관한 연구

양규호,김선희 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1

In this study, an effort was made to measure the Tweed triangle in assessing hard tissue, at the same time, to use the Z angle and Ricketts' E line in assessing facial esthetics. The cephalometric radiograms were taken from the Korean adult subjects, 21 to 27 years old, that consist of 30 males and 30 females with normal occlusion and harmonious profile, 8 items were statistically analysed. The results of this study were as follows : 1. In this study, standard values measured with 6 items in Korean were FMA 24.13˚, IMPA 94.42˚, FMIA 61.14˚, Z angle 73.95˚, Total chin 14.91mm, Upper lip thickness 12.73mm, UL to E line -1.56mm and LL to E line 0.28mm. 2. The mean of the Twed triangle in Korean males was FMA 23.95˚, IMPA 92.13˚ and FMIA 63.90˚. In females, FMA 24.32˚, IMPA 96.70˚ and FMIA 58.98˚. 3. The mean of measurements of the soft tissre in Korean males was Z angle 74.87˚, Total chin 15.05mm, Upper lip thickness 12.98mm, UL to E line -1.67mm and LL to E line 0.17mm. In females, Z angle 73.-3˚, Total chin 14.76mm and Upper lip thickness 12.48mm, UL to E line -1.45mm and LL to E line 0.40mm. 4. As compared 8 items by sex, there is no significant difference except the FMIA and IMPA among the 8 items(p<0.05). 5. As verified the correlation of the IMPA, FNIA, Z angle, UL to E line and LL to E line, there are correlations between FMIA and IMPA, FMIA and Z angle, FMIA and LL to E line, Z angle and UL to E line, Z angle and LL to E line(p<0.05). 6. As compared with the present study, Japanese and Caucasian, the mandibular incisor of Korean was proclined more than that of Caucasian, the FMA was smaller than in Japanese. The Z angle and Total chin of Korean was larger than those of Japanese, but the upper lip thickness of Korean was smaller than that of Japanese. The position of the upper lip and lower lip to Ricketts' E line in Korean adults was protruded more than those of Caucasian.

Face-Mask를 이용한 골격성 III급 부정교합 환아의 치험례

양규호,이영준 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

The conventional treatment of skeletal Class Ⅲ malocclusion has been focused on application of orthopedic force primarily to the mandible. However, In Class Ⅲ malocclusion with retrograde position or underdevelopment of Maxilla, this approach is not suitable treatment. These patients need an application of orthopedic forces via face-mask to the Maxilla to stimulate its growth and to change the direction of growth. In skeletal Class Ⅲ patients who were treated by Face-Mask, the following results were obtained. 1. Forward growth of Maxilla was enhanced. 2. Labioversion of upper incisors and linguoversion of lower incisors were observed. 3. Mandible was rotated to clockwise direction and remodeling of B point was observed. 4. Anterior crossbite was corrected by combining of the above results.

HAPS와 NGSO 시스템의 Service Link상의 간섭영향

양의장,김규환,함형일,강영흥 한국항행학회 2003 韓國航行學會論文誌 Vol.7 No.1

본 논문에서는 성층권 통신 시스템 (HAPS : High Altitude Platform System)과 NGSO (Non-Geostationary Orbit) 시스템간 간섭 영향을 두 가지의 경우로 나누어서 분석한다. 한가지는 HAPS 시스템이NGSO 시스템에 미치는 간섭이며, 다른 한가지는 NGSO위성이 HAPS시스템에 미치는 간섭 영향이다. 그 결과NGSO 이동국이 받는 간섭은 NGSO위성 위치가 95?일 때 가장 높게 나타났으며, 이때 HAPS와 NGSO모두에 의한 간섭은 사용자 수가 20명 이상부터 간섭 기준을 초과함을 알 수 있었다. 그리고 HAPS 이동국이 받는 간섭의 경우에는 NGSO 위성 양각이 87?일 때 가장 높은 간섭이 나타났으며, 이 때 NGSO와 HAPS 모두에 의한 간섭은 사용자 수가 10명 이상부터 간섭 기준을 초과함을 알 수 있었다. 또한 64?일 때 가장 낮은 간섭이 나타났으며, 이때 NGSO와 HAPS 의 사용자 수가 같을 경우에는 190명에서부터 간섭을 초과함을 알 수 있었다. In this paper, we analyze the interference effects between NGSO(Non-Geostationary Orbit) and HAPS(High Altitude Platform System) by dividing into two cases. The one is interference effects of HAPS into NGSO, the another is the interference effectes of NGSO into HAPS. At the result, we can know the interference effect into NGSO mobile station is the highest when position of NGSO is 95%. In this case, we can also know the interference effects from both HAPS and NGSO exceed interference criteria when the number of users are more than 20. And, in the case of the interference effect into HAPS mobile station, we can know it is the highest when elevation angle of NGSO is 87°, and the interference effects from both NGSO and HAPS exceed interference criteria when the number of users is more then 10. Moreover, the interference effect is the minimum, when elevation angle of NGSO is 64°, at this time, in case that the number of users of NGSO equal to the number of user of HAPS, the interference effects exceed interference criteria when the number of users is 190.

치면열구전색제와 유동성 복합 레진의 미세 경도 및 표면 조도의 비교

양규호,최남기,김선미,최지은 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

본 연구는 예방적 레진 수복술에 전통적으로 사용되던 소와열구 전색제를 대신하여, 유동성 복합 레진이 사용될 경우의 타당성을 검토하기 위해 시행되었다. 비커스 미세경도를 측정하기 위해 직경 10 mm, 높이 3 mm인 11개의 시편을 제작하여 비커스 미세경도 측정기(FM-7, FUTURE-TEC Inc., Japan)를 이용하여 압입하중 50 gf, 유지시간 15초의 조건 하에서 측정하여 각 군의 평균값을 산출하였다. 표면조도를 측정하기 위해 칫솔마모시험기(Toothbrush abrasive machine K236, Tokyo Machine Co., Japan)로 4개의 시편 표면에 200 rpm의 속도로 4000회의 칫솔질을 시행한 뒤 표면조도형상측정기(Form Tahrsurf series 2, Rank Taylor Hobson Co., England)를 이용해 표면조도를 측정하였다. α=0.05의 유의 수준에서 Kruskal-Wallis rank test와 Mann-Whitney U test를 사용하였다. 또한 주사전자현미경으로 관찰하여 각 군의 마모 양상을 비교하여 다음과 같은 결론을 얻었다. 1. 비커스 미세경도 측정 결과 Filtek Z350 flowable(3M ESPE, U.S.A), Palfique Estelite LV(Tokuyama Dental, Japan), Ultraseal XT plus(Ultradent, U.S.A) 순으로 높게 나타났으며 모든 군 간에 통계적으로 유의한 차이가 있었다(p<0.0001). 2. 표면 조도 측정 결과, Ultraseal^(R), Estelite^(R), Z350^(R) 순으로 높게 나타났으나 통계적으로 유의한 차이는 없었다(p=0.116). 3. 마모되지 않은 표면과 마모가 일어난 표면을 주사전자현미경으로 관찰한 결과, 전색제인 Ultraseal XT plus와 유동성 복합레진인 Palfique Estelite LV, 나노필러를 함유한 유동성 복합레진인 Filtek Z350 flowable이 서로 다른 마모 양상이 나타나는 것을 볼 수 있었다. 이상의 결과를 토대로 할 때, 유동성 복합 레진이 전색제에 비해 물성적 측면에서 더 우수한 것으로 나타났다. This study was performed to compare the wear resistance of sealant and flowable resins for analyzing the effect of flowable resin as a sealant in preventive resin restorations. Specimens were made and Vicker's hardness number and surface roughness were measured. SEM observations of the polished and abraded surfaces were established. Kruskal-Wallis rank test and Mann-Whitney U test at the significant level of α=0.05 were used. The following results were obtained: 1. The microhardness was decreased among groups in following order: Z350(3M ESPE, U.S.A), Estelite(Tokuyama Dental, Japan) and Ultraseal(Ultradent, U.S.A). There were significant differences in all groups(p<0.0001). 2. The surface roughness was decreased among groups in following order: Ultraseal XT plus, Palfique Estelite LV and Filtek Z350 flowable, However, there is no statistically significant differences in roughness among Estellite, Z350 and Ultraseal at the significance level of α=0,05, with p=0.116 3. SEM observation of the unworn and worn surfaces revealed the qualitative differences in the wear appearance among groups. The results in this study indicate that flowable resin is better than sealant in aspect of physical properties.

의류제품 구매에 있어서 유통채널갈등과 가격단서 지각

양원정;서민정;박재옥;이규혜 한양대학교 2006 韓國 生活 科學 硏究 Vol.26 No.2

Differentiated On Off line channel strategies are getting more attention due to conflicts between Off line channel and active E commerce. Choosing an appropriate channel strategy can prevent apparel products from clashing in market and manage each channel more efficiently. In fashion industry, product prices are flexible because of the short product life cycle and different distribution channels. It is essential to understand consumers’ apparel price cue perception when purchasing apparel in both On line and Off line. The objectives of this study was to examine the effects of apparel price cues on perceived savings and purchase intention in On line versus Off line retail channel. 303 quetionnaires from internet experienced women were used for the data analysis. Statistical analyses employed in this study were descriptive statistics, reliabilities conjoint analysis and regression. Overall, the perceived importance of apparel price cues were different between On line and Off line channels and each apparel price cue affected perceived savings and purchase intention differently according to distribution channels. Therefore, by considering characteristics of apparel price cue in On line versus Off line channels, differentiated channel strategies should be imposed to implement more effective marketing strategies.

산성 불소가 복합레진 표면에 미치는 영향

양규호,육근영,최남기,김선미 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

복합레진 수복은 치아우식 및 치아우식 예방치료를 위한 대표적인 술식으로 소아치과 영역에서 널리 시행되고 있으며 불소도포는 치아우식 예방을 위한 대표적인 술식으로 1년에 2번 정도 실시되고 있다. 하지만 이러한 이점에도 불구하고 Acidulated phosphate fluoride(APF)가 복합 레진의 표면을 거칠게 한다는 부작용에 대한 논란이 있다. 이에 본 연구에서는 불소도포가 복합레진의 표면에 미치는 영향을 평가하기 위해 시판용 레진인 Solitaire 2^(®)(Heraeus Kulzer, Germany), Metafil CX^(®)(Sun medical, Japan), Composan LCM^(®)(Promedica, Germany), Charmseal^(®)(DenKist, Korea)을 이용하여 시편을 제작하고, 불소젤은 Topex^(®)(Sultan Dental Products, Canada)를 각각 1분과 4분동안 도포시킨 후 변화를 관찰하여 다음과 같은 결과를 얻었다. 1. 불소젤을 도포시키지 않은 대조군에서 가장 평활한 표면을 보였으며 1분군, 4분군 순으로 표면 거칠기가 증가하였다. 그리고 Composan LCM^(®)을 제외한 모든 제품에서 유의한 차이를 보였다(p<0.05). 2. 국소도포용 불소젤을 도포한 각 레진 시편의 무게 차이를 비교한 결과 대조군, 1분군, 4분군 간에 유의한 차이는 없었다(p>0.05). 3. 주사전자현미경 관찰시 불소젤을 도포시킨 실험군은 대조군에 비해 거친 표면을 나타내었다. Composite resin restorations are widely used to restore decayed teeth or preventive restoration in children. Children often receive topical fluoride treatments on a semiannual basis. However there is concern that topical acidulated phosphate fluorides (APF) may cause deterioration of composite resins. The aim of this study was to evaluate the surface changes in composite resins due to topical fluoride application. Composite resins(Solitaire 2^(®), Metafil CX^(®), Composan LCM^(®), Charmseal^(®)) in topical fluoride agents were immersed and their surface roughness, weight loss and SEM findings were evaluated. The results were as follows: 1. The 4 minutes-immersion group showed more roughened surface than 1 minute-immersion group and the control group showed the smoothest surface among all the materials, and there was statistically significant difference between the groups except the Composan LCM^(®)(P<0.05). 2. There was no significant difference between the 1 minute-immersion group and 4 minutes-immersion group in weight loss (P>0.05). 3. The experimental group treated with topical fluoride gel showed generally more roughened surface than control group in the SEM findings.