Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells

Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.

Synergistic Penetration of Ethosomes and Lipophilic Prodrug on the Transdermal Delivery of Acyclovir

Yan Zhou,Yu-Hui Wei,Guo-Qiang Zhang,Xin-An Wu 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.4

The aim of this study was to investigate the lipophilic prodrug as a means of promoting acyclovir (ACV) that exhibited biphasic insolubility into the ethosomes for optimum skin delivery. Acyclovir Palmitate (ACV-C16) was synthesized as the lipophilic prodrug of ACV. The ethosomal system and the liposomal system bearing ACV or ACV-C16 were prepared, respectively. The systems were characterized for shape, zeta potential value, particle size, and entrapment efficiency. Franz diffusion cells and confocal laser scanning microscopy were used for the percutaneous absorption studies. The results showed that the entrapment efficiency of ACV-C16ethosomes (87.75%) were much higher than that of ACV ethosomes (39.13%). The quantity of drug in the skin from ACV-C16 ethosomes at the end of the 24 h transdermal experiment (622.89 μg/cm2) was 5.30 and 3.43 times higher than that from ACV-C16 hydroalcoholic solution and ACV ethosomes, respectively. This study indicated that the binary combination of the lipophilic prodrug ACV-C16 and the ethosomes synergistically enhanced ACV absorption into the skin.

HOCl Oxidation-modified CT26 Cell Vaccine Inhibits Colon Tumor Growth in a Mouse Model

Zhou, Rui,Huang, Wen-Jun,Ma, Cong,Zhou, Yan,Yao, Yu-Qin,Wang, Yu-Xi,Gou, Lan-Tu,Yi, Chen,Yang, Jin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.

Application of data mining and statistical measurement of agricultural high-quality development

Yan Zhou Techno-Press 2023 Advances in nano research Vol.14 No.3

In this study, we aim to use big data resources and statistical analysis to obtain a reliable instruction to reach high-quality and high yield agricultural yields. In this regard, soil type data, raining and temperature data as well as wheat production in each year are collected for a specific region. Using statistical methodology, the acquired data was cleaned to remove incomplete and defective data. Afterwards, using several classification methods in machine learning we tried to distinguish between different factors and their influence on the final crop yields. Comparing the proposed models' prediction using statistical quantities correlation factor and mean squared error between predicted values of the crop yield and actual values the efficacy of machine learning methods is discussed. The results of the analysis show high accuracy of machine learning methods in the prediction of the crop yields. Moreover, it is indicated that the random forest (RF) classification approach provides best results among other classification methods utilized in this study.

Research on the Factors Affecting the Willingness to Pay for Digital Music

Yan Zhou 한국컴퓨터정보학회 2019 韓國컴퓨터情報學會論文誌 Vol.24 No.6

Based on the theory of planned behavior and the theory of legal deterrence, this study takes consumers" willingness to pay for digital music as the research object, investigates the consumers who have digital music consumption channels and behaviors, and discusses the willingness of consumers to pay for digital music and its influencing factors. The study attempts to achieve the following research purposes: First, explore the influencing factors of willingness to pay for digital music using domestic and foreign literature research and related content analysis. Second, we want to examine the effect of Attitude, Collective Specifications, Quality Sensitivity and Music affinity on willingness to pay. Third, Legal deterrence and resource availability tries to verify whether there is a moderating effect between Attitude, Collective Specifications, Quality Sensitivity and Music affinity and willingness to pay. The research data was collected in 2019 between April 6th to May 8th. Questionnaires were randomly distributed in fixed places, mainly in Hubei Province, China. A total of 393 questionnaires were selected for data analysis. Based on the previous theoretical review and empirical analysis, the study draws the following conclusions: Firstly, attitude, collective specifications, quality sensitivity and music affinity have an impact on the willingness to pay. Second, Legal deterrence has a regulatory effect on the relationship among quality sensitivity, musical affinity and the willingness to pay. Last the resource availability has a significant impact on the willingness to pay. It also has a regulatory effect on the relationship among quality sensitivity, music affinity and the willingness to pay.

Field-Like Spin Torque Term Governs Enhanced Synchronization in Magnetic Tunnel Junction based Spin Torque Oscillator

Yan Zhou,Johan Akerman 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Import Demand Functions : Evidence from CIBS

YAN ZHOU;SMILE DUBE 경제연구소 2011 Journal of Economic Development Vol.36 No.4

This paper adopts the bounds testing approach to test for the validity of the cointegration or stationarity restriction embodied in five import demand model specifications for CIBS during the period 1970-2007. It identifies long-run relationships in a subset of the five models for each CIBS countries. We find that long-run income elasticities are much higher compared to earlier studies and are higher than the short-run counterparts for CIBS. In addition, contrary to the traditional wisdom, price elasticities are not significantly negative for these countries.

Study on Competitive Advantages of Convention & Exhibition Industry of Beijing Capital Region (Jing-Jin-Ji Area) in China

Yan Zhou,Chuncai Wang 한국무역전시학회 2014 무역전시연구 Vol.9 No.3

Convention industry is one of the fastest growing segments in China in the last decades. As the capital city in China, Beijing became a leading convention city, and the industrial growth reported is around 20% per year. It has been more than 20 years since the regional tourism cooperation started in the Jing-Jin-Ji Economic Zone; however, the performance has not been so satisfactory because of various reasons. How to strengthen the cooperation in this field to better promote regional economic development is not only an important task for practice, but also one area of a focus in the academic field. There are three economic zones in China, which are Jing-Jin-Ji, Yangtze River Delta and Pearl River Delta, where the convention industry is also a booming sector of the economy. The article reviews the resources and attributes of these areas, and analyzes the regional competitive advantages in terms of some key indicators like venues, PEO, star hotels, international meetings and tourist attractions. Some suggestions are given too.

Molecular Cloning and Characterization of a Novel Exo-β-1,3-Galactanase from Penicillium oxalicum sp. 68

Zhou, Tong,Hu, Yanbo,Yan, Xuecui,Cui, Jing,Wang, Yibing,Luo, Feng,Yuan, Ye,Yu, Zhenxiang,Zhou, Yifa The Korean Society for Microbiology and Biotechnol 2022 Journal of microbiology and biotechnology Vol.32 No.8

Arabinogalactans have diverse biological properties and can be used as pharmaceutical agents. Most arabinogalactans are composed of β-(1→3)-galactan, so it is particularly important to identify β-1,3-galactanases that can selectively degrade them. In this study, a novel exo-β-1,3-galactanase, named PoGal3, was screened from Penicillium oxalicum sp. 68, and hetero-expressed in P. pastoris GS115 as a soluble protein. PoGal3 belongs to glycoside hydrolase family 43 (GH43) and has a 1,356-bp gene length that encodes 451 amino acids residues. To study the enzymatic properties and substrate selectivity of PoGal3, β-1,3-galactan (AG-P-I) from larch wood arabinogalactan (LWAG) was prepared and characterized by HPLC and NMR. Using AG-P-I as substrate, purified PoGal3 exhibited an optimal pH of 5.0 and temperature of 40℃. We also discovered that Zn²⁺ had the strongest promoting effect on enzyme activity, increasing it by 28.6%. Substrate specificity suggests that PoGal3 functions as an exo-β-1,3-galactanase, with its greatest catalytic activity observed on AG-P-I. Hydrolytic products of AG-P-I are mainly composed of galactose and β-1,6-galactobiose. In addition, PoGal3 can catalyze hydrolysis of LWAG to produce galacto-oligomers. PoGal3 is the first enzyme identified as an exo-β-1,3-galactanase that can be used in building glycan blocks of crucial glycoconjugates to assess their biological functions.

Human Visual System based Automatic Underwater Image Enhancement in NSCT domain

( Yan Zhou ),( Qingwu Li ),( Guanying Huo ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.2

Underwater image enhancement has received considerable attention in last decades, due to the nature of poor visibility and low contrast of underwater images. In this paper, we propose a new automatic underwater image enhancement algorithm, which combines nonsubsampled contourlet transform (NSCT) domain enhancement techniques with the mechanism of the human visual system (HVS). We apply the multiscale retinex algorithm based on the HVS into NSCT domain in order to eliminate the non-uniform illumination, and adopt the threshold denoising technique to suppress underwater noise. Our proposed algorithm incorporates the luminance masking and contrast masking characteristics of the HVS into NSCT domain to yield the new HVS-based NSCT. Moreover, we define two nonlinear mapping functions. The first one is used to manipulate the HVS-based NSCT contrast coefficients to enhance the edges. The second one is a gain function which modifies the lowpass subband coefficients to adjust the global dynamic range. As a result, our algorithm can achieve contrast enhancement, image denoising and edge sharpening automatically and simultaneously. Experimental results illustrate that our proposed algorithm has better enhancement performance than state-of-the-art algorithms both in subjective evaluation and quantitative assessment. In addition, our algorithm can automatically achieve underwater image enhancement without any parameter tuning.