Advanced polymer hydrogels that promote diabetic ulcer healing: mechanisms, classifications, and medical applications

Yamei Xu,Qiyuan Hu,Zongyun Wei,Yi Ou,Youde Cao,Hang Zhou,Mengna Wang,Kexiao Yu,Bing Liang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Diabetic ulcers (DUs) are one of the most serious complications of diabetes mellitus. The application of a functional dressing is a crucial step in DU treatment and is associated with the patient’s recovery and prognosis. However, traditional dressings with a simple structure and a single function cannot meet clinical requirements. Therefore, researchers have turned their attention to advanced polymer dressings and hydrogels to solve the therapeutic bottleneck of DU treatment. Hydrogels are a class of gels with a three-dimensional network structure that have good moisturizing properties and permeability and promote autolytic debridement and material exchange. Moreover, hydrogels mimic the natural environment of the extracellular matrix, providing suitable surroundings for cell proliferation. Thus, hydrogels with different mechanical strengths and biological properties have been extensively explored as DU dressing platforms. In this review, we define different types of hydrogels and elaborate the mechanisms by which they repair DUs. Moreover, we summarize the pathological process of DUs and review various additives used for their treatment. Finally, we examine the limitations and obstacles that exist in the development of the clinically relevant applications of these appealing technologies.

Optimal Routing Strategy on Scale-free Networks with Heterogeneous Delivering Capacity

Zou Yamei,Li Zuoming 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.1

We proposed the information traffic dynamics on scale-free networks considering the heterogeneous delivering capacity. In the previous researches, the delivering capacity is the same for all nodes in the system, which obviously contradicts the real observations. In this paper, a heuristic algorithm for the optimization of transport is proposed to enhance traffic efficiency in complex networks, where each node's capacity is set as ci = ki and ki is the degree of node i. Our algorithm balances traffic on a scale-free network by minimizing the maximum effective betweenness, which can avoid or reduce the overload in some busy nodes. The simulation result shows that the network capacity can reach a very high value, which is four times more than that of the efficient routing strategy. The distribution of traffic load is also studied and it is found that our optimal routing strategy can make a good balance between hub nodes and non-hub nodes, resulting in high network capacity.

Estrogen negatively regulates the renal epithelial sodium channel (ENaC) by promoting Derlin-1 expression and AMPK activation

Xue Zhang,Yamei Ge,Ashfaq-Ahmad-Shah Bukhari,Qian Zhu,Yachen Shen,Min Li,Hui Sun,Dongming Su,Xiubin Liang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The main functions of the epithelial sodium channel (ENaC) in the kidney distal nephron are mediation of sodium and water balance and stabilization of blood pressure. Estrogen has important effects on sodium and water balance and on premenopausal blood pressure, but its role in the regulation of ENaC function is not fully understood. Female Sprague–Dawley rats were treated with 17β-estradiol for 6 weeks following bilateral ovariectomy. Plasma estrogen, aldosterone, creatinine, and electrolytes were analyzed, and α-ENaC and derlin-1 protein expression in the kidney was determined by immunohistochemistry and western blotting. The expression levels of α-ENaC, derlin-1, AMPK, and related molecules were also examined by western blotting and real-time PCR in cultured mouse renal collecting duct (mpkCCDc14) epithelial cells following estrogen treatment. Immunofluorescence and coimmunoprecipitation were performed to detect α-ENaC binding with derlin-1 and α-ENaC ubiquitination. The results demonstrated that the loss of estrogen elevated systolic blood pressure in ovariectomized (OVX) rats. OVX rat kidneys showed increased α-ENaC expression but decreased derlin-1 expression. In contrast, estrogen treatment decreased α-ENaC expression but increased derlin-1 expression in mpkCCDc14 cells. Moreover, estrogen induced α-ENaC ubiquitination by promoting the interaction of α-ENaC with derlin-1 and evoked phosphorylation of AMPK in mpkCCDc14 cells. Our study indicates that estrogen reduces ENaC expression and blood pressure in OVX rats through derlin-1 upregulation and AMPK activation.

Small Double-Stranded RNA Mediates the Anti-Cancer Effects of p21WAF1/ClP1 Transcriptional Activation in a Human Glioma Cell Line

Zhiqiang Dong,Yamei Dang,Yirong Chen 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.2

Purpose: This study was conducted to investigate the small double-stranded RNA (dsRNA) mediated anti-tumor effects of p21WAF1/ClP1 (p21) transcriptional activation in vitro in the human glioma SHG-44 cell line. Materials and Methods: Human glioma SHG-44 cells were transfected with dsRNA using LipofectAMINE 2000 transfection reagent. Real-time PCR and Western blot analysis were conducted to detect p21 and survivin mRNA and protein levels, respectively. Cell proliferation was examined by MTT assay. Cell cycle distribution and apoptosis were detected by flow-cytometric analysis. Results: We found that dsRNA targeting p21 promoter (dsP21) significantly induced the expression of p21 at transcription and protein levels,and reduced the expression of survivin. AS well, dsP21 transcription significantlyinhibited human glioma SHG-44 cell proliferation. Analysis of cell cycle distributionrevealed that dsP21 transfection increased accumulation of cells in the G0/G1 phase and reduced accumulation of cells in the S phase. Further analysis revealed that dsP21 transcription led to an increase in both early and late stages of apoptosis in human glioma SHG-44 cells. Conclusion: In the present study, P21 activation by RNA-induced gene activation (RNAa) induced anti-tumor activity in vitro in a humanglioma SHG-44 cell line. The results suggested that RNAa could be used for human glioma treatment by targeted activation of tumor suppressor genes.

Genome-wide Transcriptome Profiling Reveals the Mechanism of the Effects of Uniconazole on Root Development in Glycine Max

Yiqiang HAN,Yamei GAO,Ying Shi,Jidao Du,Dianfeng Zheng,Guifeng Liu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.4

Uniconazole, a plant growth retardant, possessesthe ability to improve quality and increase tolerance of plant. However, it is known little about the effects of uniconazoleon root. In this study, uniconazole treatments that were appliedthrough seed soaking, promoted soybean root development,and the microstructure of root showed increase of corticalthickness and cortex cell width. Meanwhile, the endogenoushormone content also altered in root after uniconazoletreatments. To obtain the molecular mechanism underlyingthe effects of uniconazole on root, we performed an RNAseqof roots harvested 3 days after uniconazole treatment. Through analyses of phytohormone-associated genes forendogenous hormones changes, we found that not only GAbiosynthesis pathway but also the regulation genes of thepathway were affected. Above all, the dominant pathwayplant hormone signal transduction may be the main factor ofthe cambium proliferation, in especial ethylene/ERF signalingpathway. Moreover, the transcriptome demonstrated differentiallyexpressed genes that determined cell division and cell wallmodification may be regulators of root growth. CLE signalingand receptor-like kinases may play a crucial role in the rootelongation. Besides, 177 transcription factors (TFs) wereinvolved in response to uniconazole. Taken together, allthese findings provide insights into the complex molecularmechanisms associated with root development after uniconazoletreatment.

Optimal Power Allocation for Wireless Uplink Transmissions Using Successive Interference Cancellation

( Liaoyuan Wu ),( Yamei Wang ),( Jianghong Han ),( Wenqiang Chen ),( Lusheng Wang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.5

Successive interference cancellation (SIC) is considered to be a promising technique to mitigate multi-user interference and achieve concurrent uplink transmissions, but the optimal power allocation (PA) issue for SIC users is not well addressed. In this article, we focus on the optimization of the PA ratio of users on an SIC channel and analytically obtain the optimal PA ratio with regard to the signal-to-interference-plus-noise ratio (SINR) threshold for successful demodulation and the sustainable demodulation error rate. Then, we design an efficient resource allocation (RA) scheme using the obtained optimal PA ratio. Finally, we compare the proposal with the near-optimum RA obtained by a simulated annealing search and the RA scheme with random PA. Simulation results show that our proposal achieves a performance close to the near-optimum and much higher performance than the random scheme in terms of total utility and Jain`s fairness index. To demonstrate the applicability of our proposal, we also simulate the proposal in various network paradigms, including wireless local area network, body area network, and vehicular ad hoc network.

Selective Electrocatalytic Reduction of Nitrite to Dinitrogen Based on Decoupled Proton–Electron Transfer

He, Daoping,Li, Yamei,Ooka, Hideshi,Go, Yoo Kyung,Jin, Fangming,Kim, Sun Hee,Nakamura, Ryuhei American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.6

<P>The development of denitrification catalysts which can reduce nitrate and nitrite to dinitrogen is critical for sustaining the nitrogen cycle. However, regulating the selectivity has proven to be a challenge, due to the difficulty of controlling complex multielectron/proton reactions. Here we report that utilizing sequential proton–electron transfer (SPET) pathways is a viable strategy to enhance the selectivity of electrochemical reactions. The selectivity of an oxo-molybdenum sulfide electrocatalyst toward nitrite reduction to dinitrogen exhibited a volcano-type pH dependence with a maximum at pH 5. The pH-dependent formation of the intermediate species (distorted Mo(V) oxo species) identified using operando electron paramagnetic resonance (EPR) and Raman spectroscopy was in accord with a mathematical prediction that the p<I>K</I><SUB>a</SUB> of the reaction intermediates determines the pH-dependence of the SPET-derived product. By utilizing this acute pH dependence, we achieved a Faradaic efficiency of 13.5% for nitrite reduction to dinitrogen, which is the highest value reported to date under neutral conditions.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2018/jacsat.2018.140.issue-6/jacs.7b12774/production/images/medium/ja-2017-12774b_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja7b12774'>ACS Electronic Supporting Info</A></P>

Fracture process of rubberized concrete by fictitious crack model and AE monitoring

Chao Wang,Zhe Zhao,Yamei Zhang 사단법인 한국계산역학회 2012 Computers and Concrete, An International Journal Vol.9 No.1

According to the results of three-point bending tests of rubberized concrete and plain concrete, the parameters such as total fracture energy (GF), initial fracture energy (Gf), and tensile strength (ft) are obtained for concrete material. Using ABAQUS software and a bilinear softening fictitious crack model, the crack propagation process was simulated and compared to the experimental results. It is found that the increase of AE hit count has a similar trend with the increase of energy dissipation in FEM simulation. For two types of concretes, both experimental results and numerical simulation indicate that the rubberized concrete has a better fracture resistance.

The Image Multi Feature Retrieval based on SVM Semantic Classification

Che Chang,Yu Xiaoyang,Bai Yamei 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.3

The image retrieval performance based on single feature is limited. For different kinds of images, it can not a better retrieval result. This paper raises image retrieval method based on weighted multi feature. In each kind of images, each feature precision is the weight evidence. On this basis, we research the existing semantic retrieval technology. Choosing the SVM classification theory which is more mature. Selecting parts of images as training set. Doing the training classification. From the research of different characteristics priority, it raises the image retrieval technology which synthesizes SVM and multi feature. From that, it can get a higher retrieval efficiency.

Anti-oxidative-initiated cognitive impairment amelioration in Alzheimer's disease model rats through preventive transcutaneous electrical acupoint stimulation

Heng Weng,Qing Wang,Ran Ye,Yamei Bai,Hui Yang,Guihua Xu,Qiuqin Wang 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.2

Background: Alzheimer's disease (AD) is a chronic and irreversible neurodegenerative disease. Oxidative stress emerges at the early AD stage. As a non-invasive therapy with few adverse reactions, transcutaneous electrical acupoint stimulation (TEAS) combines acupuncture points of traditional Chinese medicine (TCM) and electrical stimulation. This study aimed to investigate the amelioration effects of preventive TEAS treatment (P-TEAS) on cognitive impairment and oxidative stress in AD model rats. Methods: The AD model was established via subcutaneous injections of D-galactose (D-gal, 120 mg/kg/d) into the back of neck for 9 weeks in Sprague Dawley (SD) rats to simulate the oxidative stress in the early AD stage. On the first day of the 10th week, Aβ1–42 (1 μg/μl) was injected into the CA1 regions of the bilateral hippocampus. P-TEAS was synchronized from the first day of subcutaneous D-gal injections for 9 weeks. Results: Empirical measurements showed that P-TEAS can improve the spatial memory ability of AD model rats in the Morris water maze. Superoxide dismutase (SOD) was upregulated in the P-TEAS group. Through the detection of the anti-oxidative stress signaling pathway, namely, Kelch-like ECH-associated protein 1 (Keap1)/ NFE2-related factor 2 (Nrf2), it was found that P-TEAS could promote Nrf2 entering into the nucleus and upregulating the production of protective factors heme oxygenase 1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1). It was also found that P-TEAS could downregulate the expressions of BCL2-associated X-protein (Bax), caspase 3, and caspase 9 to inhibit neuronal apoptosis. Conclusions: P-TEAS has similar efficacy to electroacupuncture in preventing AD occurrence and development. P-TEAS is a new non-invasive intervention therapy for the prevention of AD.