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Xue‑Lian Song,Fei‑Fei Zhang,Wen‑Jing Wang,Xin‑Ning Li,Yi Dang,Ying‑Xiao Li,Qian Yang,Mei‑Jing Shi,Xiao‑Yong Qi 한국유전학회 2020 Genes & Genomics Vol.42 No.12
Background: Myocardial ischemia and reperfusion injury (MI/RI) is a complex pathophysiological process, which can lead to severe myocardial injury. The long noncoding RNA alpha-2-macroglobulin antisense RNA 1 (A2M-AS1) has been revealed to be abnormally expressed in MI, However, its function in MI and the potential mechanism are still unclear. Objective: To evaluate the functional role of A2M-AS1 in hypoxia/reoxygenation (H/R)-induced neonatal cardiomyocytes and its potential molecular mechanism. Methods: Dataset GSE66360 was obtained from GEO database for analyzing the RNA expression of A2M-AS1 and interleukin 1 receptor type 2 (IL1R2). KEGG pathway enrichment analysis of the genes that co-expressed with A2M-AS1 was performed. Human neonatal cardiomyocytes were subjected to H/R to construct in vitro models. QRT-PCR and Western blot were adopted to test the levels of mRNA and protein. The viability and apoptosis of cardiomyocytes were tested by CCK-8 and flow cytometry assays, respectively. Results: The expression of A2M-AS1 was notably downregulated in H/R-treated cardiomyocytes. Overexpression of A2M-AS1 can notably enhance the cell viability of H/R-damaged cardiomyocytes, whereas knockdown of A2M-AS1 showed the opposite outcomes. Besides, a negative correlation was showed between A2M-AS1 and IL1R2 expression. In H/R-treated cardiomyocytes, overexpression of IL1R2 weakened the promoting proliferation and anti-apoptosis effects caused by overexpressing A2M-AS1, however, IL1R2-knockdown abolished the anti-proliferation and pro-apoptosis effects caused by silencing A2M-AS1. Conclusion: This study demonstrates the potential regulatory role of A2M-AS1/ IL1R2 axis in cardiomyocytes suffered from H/R, and provides insight into the protection of MI/RI.
회수수에 처리되는 당류계 안정화제의 혼입률 변화가 콘크리트의 품질에 미치는 영향
文學龍,金虎林,우종완,한민철,한천구 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)
The objective of present paper is to investigate the engineering properties of concrete using recycling water incorporating saccharic based stabilizing agent in order to improve qualities of concrete in the region of normal strength and high strength. According to test results, fluidity and air content do not exhibit significant difference with an increase in stabilizing agent contents. Bleeding was not observed at 30% of W Ie, regardless of stabilizing agent contents, nonetheless, concrete with stabilizing agent of 0.15% was observed to occur the least bleeding at 40 and 50% of W IC. For setting time, although presence of recycled water accelerates setting time, setting time shows to be retarded gradually with an increase in stabilizing agent content. For compressive strength, use of stabilizing agent enhanced compressive strength of concrete compared with that of concrete without stabilizing agent. In the side of strength enhancement, adequate dosage of stabilizing agent is found to be 0.15%. As dosage of stabilizing agent increased above 0.15%, drying shrinkage exhibited a tendency to decrease.
시멘트의 분말도 변화에 따른 시멘트 모르터의 역학적 특성
文學龍,김경민,전충근,윤기원,양성환,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2
This study discusses the influence of fineness of cement on mechanical properties of cement mortar. According to the result, compressive strength increases in order of C>B>OPC>A and C>B>A>OPC at mixture proportion of 1:1 and 1:3 respectively. An increase of compressive strength by increase of fineness of cement is because internal structure of concrete grows dense by an increase of hydration reaction. As for curing condition, compressive strength is higher in standard curing than in air curing at mixture proportion of 1:1 and 1:3. There is not any tendency of strength development with age and fineness of cement by 91days. Length change by drying shrinkage increases in order of C>B>OPC>A at mixture proportion of 1:1 and 1:3 due to an increase of water content by variation of W/C.
文學龍,裵正烈,황인성,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)
This study is to investigate the influence of W/C and fineness modulus of aggregate on the bleeding of concrete. According to the result, the lower W/C is, the less bleeding occurs. As for the properties of bleeding with the variation of fineness modulus, the amount of bleeding is small with the continuous grading in the middle of the standard grading range in the case of coarse aggregate, and it grows smaller with a decrease of fineness modulus in the case of fine aggregate. Therefore, it proves that using coarse aggregate with the continuous grading in the middle of the standard grading range and fine aggregate with smaller fineness modulus in the standard grading range is the effective method for reducing bleeding at the same W/C.
황인성,文學龍,金光華,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)
This study is to investigate the influence of contents of mineral admixture on bleeding of concrete. According to the results, as W/B increases, bleeding occurs rapidly at early age regardless of replacement of mineral admixture, and when slump and air content are kept equal, the amount of bleeding decreases proportionately with an increase of replacing ratio of fly ash and blast furnace slag powder. Bleeding speed is highest at 60 minutes after placing concrete, and grow slower after 180 minutes. Bleeding speed also grows high with an increase of W/B, and is lower in the case of fly ash than in the case of blast furnace slag powder.
운반시간 지연에 의해 저하된 레미콘의 성능회복에 관한 기초적 연구
전충근,文學龍,황인성,양성환,한천구 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)
In this paper, availability of deteriorated remicon due to delaying delivery time is discussed. According to test results, fluidity and air content of plain concrete exhibited a tendency to decline with a elapse of time. After 90 minutes since mixed, use of superplasticizer and AE admixture resulted in an increase in fluidity and air content. Adequate dosage of superplasticizer and AE adimixture is found to add with 0.4% and 0.002% respectively to restore the level of original slump and air content from loss one. Setting time of concrete exhibited an acceleration with an increase in leaving time of concrete. Compressive strength of plain concrete is enhanced with and increase of leaving time of concrete. Therefore, it is found that the use of superplasticizer with 0.4% and AE admixture with 0.002% has a desirable effect on restoring the deteriorated quality of concrete related to fluidity, air content and setting time without strength loss caused by delivery time delaying.
콘크리트의 블리딩에 미치는 시멘트 제조사 및 분말도의 영향
金恩浩,文學龍,황인성,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)
This study is to investigate the influence of the manufacturing company and fineness of cement on bleeding of concrete. According to the results, as fineness of comment increases, slump and air content of fresh concrete decrease, and setting time is shortened. The amount of bleeding abates with an increase of fineness, and it is confirmed that bleeding does not occur especially in the case of B8000. it is confirmed that bleeding does not occur. Bleeding speed is higher in the case of OPC A than in the case OPC B, and grows low with an increase of fineness. It also becomes highest at about 60 minutes, and shows slow tendency in W/C of 45% in comparison with W/C of 50%. And It is confirmed that relativity between fineness of cement and the final amount of bleeding is very favorable. As the properties of hardened concrete, compressive strength do not make much difference between OPC A and B is little, and increases with an increase of fineness.
Genomic Screening for Targets Regulated by Berberine in Breast Cancer Cells
Wen, Chun-Jie,Wu, Lan-Xiang,Fu, Li-Juan,Yu, Jing,Zhang, Yi-Wen,Zhang, Xue,Zhou, Hong-Hao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Berberine, a common isoquinoline alkaloid, has been shown to possess anti-cancer activities. However, the underlying molecular mechanisms are still not completely understood. In the current study, we investigated the effects of berberine on cell growth, colony formation, cell cycle distribution, and whether it improved the anticancer efficiency of cisplatin and doxorubicin in human breast cancer estrogen receptor positive (ER+) MCF-7 cells and estrogen receptor negative (ER-) MDA-MB-231 cells. Notably, berberine treatment significantly inhibited cell growth and colony formation in the two cell lines, berberine in combination with cisplatin exerting synergistic growth inhibitory effects. Accompanied by decreased growth, berberine induced G1 phase arrest in MCF-7 but not MDA-MB-231 cells. To provide a more detailed understanding of the mechanisms of action of berberine, we performed genome-wide expression profiling of berberine-treated cells using cDNA microarrays. This revealed that there were 3,397 and 2,706 genes regulated by berberine in MCF-7 and MDA-MB-231 cells, respectively. Fene oncology (GO) analysis identified that many of the target genes were involved in regulation of the cell cycle, cell migration, apoptosis, and drug responses. To confirm the microarray data, qPCR analysis was conducted for 10 selected genes based on previously reported associations with breast cancer and GO analysis. In conclusion, berberine exhibits inhibitory effects on breast cancer cells proliferation, which is likely mediated by alteration of gene expression profiles.
( Wen-wen Zhang ),( Xue-fei Zhou ),( Ya-lei Zhang ),( Peng-fei Cheng ),( Rui Ma ),( Wen-long Cheng ),( Hua-qiang Chu ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Natural astaxanthin mainly derives from a microalgae producer, Haematococcus pluvialis. The induction of nitrogen starvation and high light intensity is particularly significant for boosting astaxanthin production. However, the different responses to light intensity and nitrogen starvation needed to be analyzed for biomass growth and astaxanthin accumulation. The results showed that the highest level of astaxanthin production was achieved in nitrogen starvation, and was 1.64 times higher than the control group at 11 days. With regard to the optimization of light intensity utilization, it was at 200 μmo/m2/s under nitrogen starvation that the highest astaxanthin productivity per light intensity was achieved. In addition, both high light intensity and a nitrogen source had significant effects on multiple indicators. For example, high light intensity had a greater significant effect than a nitrogen source on biomass dry weight, astaxanthin yield and astaxanthin productivity; in contrast, nitrogen starvation was more beneficial for enhancing astaxanthin content per dry weight biomass. The data indicate that high light intensity synergizes with nitrogen starvation to stimulate the biosynthesis of astaxanthin.
( Wen Fei Xue ),( Jing Fu Peng ),( Xiao Li Yu ),( Shu Lin Zhang ),( Boping Zhou ),( Dan Qing Jiang ),( Jian Bo Chen ),( Bing Bing Ding ),( Bin Zhu ),( Yao Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem- PCR) was applied to avoid interference between different primers in conventional multiplex- PCR. We applied this assay to 276 clinical specimens (including 27 sputum, 4 alveolar lavage fluid, 2 pleural effusion, and 243 culture isolate specimens; 40 of the 276 were non-tuberculosis mycobacteria specimens and 236 were M. tuberculosis specimens). The testing process took 4.5 h. A sensitivity of 50 copies per PCR was achieved, while the sensitivity was 500 copies per PCR when tem-PCR was used. Allele sequences could be detected in mixed samples at aproportion of 10%. Detection results showed a concordance rate of 97.46% (230/236) in rifampicin resistance detection (sensitivity 95.40%, specificity 98.66%) and 96.19% (227/236) in isoniazid (sensitivity 93.59%, specificity 97.47%) detection with those of DST assay. Concordance rates of testing results for sputum, alveolar lavage fluid, and pleural effusion specimens were 100%. The assay provides a potential choice for TB diagnosis and treatment.