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( Xue Liang Niu ),( Xiao Ran Hao ),( Zhang Yong Hong ),( Long Fei Chen ),( Xi Yu ),( Xu Dong Zhu ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5
Fungi of the genus Pestalotiopsis have drawn attention for their capability to produce an array of bioactive secondary metabolites that have potential for drug development. Here, we report the determination of a polyketide derivative compound, pestalotiollide B, in the culture of the saprophytic fungus Pestalotiopsis microspora NK17. Structural information acquired by analyses with a set of spectroscopic and chromatographic techniques suggests that pestalotiollide B has the same skeleton as the penicillide derivatives, dibenzodioxocinones, which are inhibitors of cholesterol ester transfer protein (CETP), and as purpactins A and C’, inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT). Strain NK17 can make a fairly high yield of pestalotiollide B (i.e., up to 7.22 mg/l) in a constitutive manner in liquid culture. Moreover, we found that a putative histone deacetylase gene, designated as hid1, played a role in the biosynthesis of pestalotiollide B. In the hid1 null mutant, the yield of pestalotiollide B increased approximately 2-fold to 15.90 mg/l. In contrast, deletion of gene hid1 led to a dramatic decrease of conidia production of the fungus. These results suggest that hid1 is a modulator, concerting secondary metabolism and development such as conidiation in P. microspora. Our work may help with the investigation into the biosynthesis of pestalotiollide B and the development for new CETP and ACAT inhibitors.
Xue-Liang Liu,Hai-Hui Ye,Hui-Yang Huang,Jie Gong,Ya-Nan Yang 한국유전학회 2014 Genes & Genomics Vol.36 No.1
Large conductance calcium-activated potassiumchannels (Slo) play important roles in controllingneuronal excitability. At present, very little is known aboutthe function of Slo channels on ovarian development. Wecloned the SPSlo gene from the mud crab, Scylla paramamosain. This gene shows 91 and 93 % sequence identityto PISlo from the spiny lobster, Panulirus interruptus andCBSlo from the jonah crab, Cancer borealis, respectively. We isolated six variants of the SPSlo cDNA within S. paramamosain ovary tissue. Sequence analysis indicatedthat there were at least seven alternative sites in SPSlo,each with multiple alternative segments. Real-time PCRshowed that the SPSlo gene was expressed in various tissues,and highly expressed in brain and ovary. In addition,the expression of SPSlo changed throughout ovariandevelopment, highest at the early-developing stage (StageII) followed by a slow decrease in subsequent stages. Theseresults suggested that SPSlo channels may be implicated inthe ovarian development of the mud crab.
장이머우 감독 영화의 색채 미학 연구 -<붉은 수수밭>, <홍등>, <영웅>을 중심으로-
량쉬에칭 ( Xue-qing Liang ) 한국영화교육학회 2023 영화교육연구 Vol.17 No.3
미학 형식으로 존재하는 영화 색채는 영화 예술을 구성하는 요소 중의 하나이다. 5세대 감독의 선두 주자 중 한 명인 장이머우 감독은 색채를 매우 잘 사용하여 화면의 내포를 풍부하게 하고 빨간색을 사랑한다. 본 연구는 장이머우 감독의 대표적인 세 작품인 <붉은 수수밭>, <홍등>, <영웅>을 선정하였다. 도상학, 색채 심리학 등 관련 지식을 활용하여 빨간색이 영화에서 상징적 의미와 민족적 가치를 분석하고 영화에서 색채의 시각적 과잉과 구축된 영화의 시각적 식 별도를 분석한다. 장이머우 감독이 색채 운용과 미학 표현에서 끊임없이 돌파하고 혁신하는 것을 더욱 체득하기 위한 것이다. The aesthetic form of film color is one of the elements that constitute film art. As one of the leaders of the fifth generation directors, Director Zhang Yimou is very good at using colors, enriching the content of the screen, and loves red. This study selected three representative works directed by Zhang Yimou, namely Red Sorghum Land, Red Lantern, and Hero. Using relevant knowledge such as graphics and color psychology, analyze the symbolic significance and national value of red in movies, analyze the visual excess of color in movies, and construct visual recognition of movies. The aim is to further experience Director Zhang Yimou's continuous breakthroughs and innovations in color application and aesthetic expression.
Estimation of Sensible Heat Flux using Thermal Infrared Data in the Arid Area
Guo,Xue-Liang,Chung,Yong-Seung 한국교원대학교 환경과학연구소 2000 환경연구논문집 Vol.- No.5
중국 서부의 사막과 오아시스 지역에서 eddy 상관 방법을 이용 현열 flux (H)와 지면의 열적외선 온도 (??) 관측을 수행하여 H와 ??-??(??2m 높이의 기온)의 관계를 정량화 하였다. 연구결과에 의하면, 오아시스와 사막지역의 불안정한 대기조건에서는 ??-??차이로부터 H를 계산 할 수 있는 간단한 관계가 이용될 수 있다. 풍속이 강할 때는 H와 ??-??관계가 풍속에 큰 영향을 받는다. 이에 관련된 풍속의 효과는 열적외선 자료를 이용하여 현열 flux의 정확한 산출에 활용될 수 있음이 제안되었다. The relationship between H and (??-??) (?? being the air temperature at 2 m) was established in this study from the measurements of eddy fluxes of sensible heat (H), and surface thermal infrared temperature (??) both in desert and oasis area of western China. Results show that under unstable condition a simple relationship can be used to estimate H from the difference(??-??) overoasis area and desert region. The relationship between H and(??-??) was greatly influenced when wind speed was high. The effect of wind speed on this relationship was suggested to consider in accurate estimation of sensible heat flux using thermal infrared data.
Guo,Xue-Liang,Huang,Mei-Yuan,Chung,Yong-Seung,Zhou,Ling 한국교원대학교 환경과학연구소 2000 환경연구논문집 Vol.- No.5
중국 북부지방의 관측자료와 강우 category model을 이용하여 강수 발생의 미세과정과 전형적인 층운 3가지 종류가 형성될 때의 빙정 역할이 분석되었다. 종류 Ⅰ는 강한 전선면의 광범위한 상승에 따른 층운의 형성으로서 강우의 발생은 주로 수증기의 침전과 상층 구름 속 빙정위의 riming과정에 좌우된다. 이때 빙정은 높은 구름에 매우 큰 농도로 존재하여 강수의 형성에 중요한 역할을 하며 이 구름 종류의 seeder-feeder 과정으로 고려된다. 종류 Ⅱ는 저기압 규모의 수렴에 의해 형성되는 층운으로서 riming에 의해 성장되는 빙정과정에 따라 발생되는 강수이다. 종류 Ⅲ은 약한 전선면상에 발생되는 광범위한 상승 기류에 동반되어 낮은 구름층에서 형성된다. 구름 방울들의 충돌과 구름 방울 및 빗방울 사이의 충돌은 이런 강우 형성의 중요 과정임이 밝혀졌다. Based on the observations in northern China, the microphysical process of precipitation formation and the role of ice crystal playing in this process for three kinds of typical stratiform clouds are analyzed using rain category model. Results show that for type one, namely stratiform cloud system formed by widespread lifting of a strong frontal surface, the original formation of rainfall depends mainly on vapor deposition and riming process on ice particles from aloft. Ice crystal has a high number concentration in high level of cloud due to the lower temperature and plays a very important role in the formation of precipitation and shows clearly the "seeder-feeder" process in this kind of cloud; For type two, namely stratiform cloud system produced by cyclonic scale convergence which provides liquid water, the primary precipitation is due to the process of ice crystal growing by riming; Type three is the shallow layer cloud system associated with widespread lifting of a weak frontal surface system.