Influence of host plants on the development, survivorship, and fecundity of the summer fruit tortrix moth, Adoxophyes orana (Lepidoptera: Tortricidae)

Li Guang‐wei,Wang Hui‐min,Yang Wen‐tao,Chen Xiu‐lin,Li Bo‐liao,Chen Yu‐xin 한국곤충학회 2021 Entomological Research Vol.51 No.10

The summer fruit tortrix moth Adoxophyes orana is a seriously damaged leaf roller pest for fruit trees. In this study, we evaluated the effect of five host plants (apple, Malus domestica B.; peach, Prunus persica var. nectarina; apricot, Armeniaca vulgaris Lam.; mulberry, Morus alba L.; and jujube, Ziziphus jujuba M.) on the development, survival, and fecundity of A. orana under laboratory conditions. Results showed that the development times of A. orana on jujube were significantly longer than those on the other four plants. The preimaginal survival rate was influenced by the host plants, which were fed during the larval stage, and with the highest on peach, followed by apple, apricot, and mulberry, and with the lowest on jujube. Adult females from larvae reared on apricot laid the greatest numbers of eggs (318.11 eggs per female), while that on apple, jujube, and mulberry was smaller, and no significant difference was observed among them. The highest intrinsic rate of increase (rm) was observed on peach and apricot; these two fruit trees were considered to be the most suitable host plant for A. orana because of the shorter developmental duration, higher survival rate, and the highest fecundity.

Concurrent Weekly Docetaxel Chemotherapy in Combination with Radiotherapy for Stage III and IVA-B Nasopharyngeal Carcinoma

Wei, Wei-Hong,Cai, Xiu-Yu,Xu, Tao,Zhang, Guo-Yi,Wu, Yong-Feng,Feng, Wei-Neng,Lin, Li,Deng, Yan-Ming,Lu, Qiu-Xia,Huang, Zhe-Li Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Background and Purpose: Cisplatin is the most common chemotherapeutic agent for loco-regionally advanced nasopharyngeal carcinoma (NPC); however, toxicity is a limiting factor for some patients. We retrospectively compared the efficacy and toxicity of weekly docetaxel-based and cisplatin-based concurrent chemoradiotherapy in loco-regionally advanced NPC. Methods and Materials: Eighty-four patients with Stage III and IVA-B NPCs, treated between 2007 and 2008, were retrospectively analyzed. Thirty received weekly docetaxel-based concurrent chemotherapy, and 43 were given weekly cisplatin-based concurrent chemotherapy. Radiotherapy was administered using a conventional technique (seven weeks, 2.0 Gy per fraction, total dose 70-74 Gy) with 6-8 Gy boosts for some patients with locally advanced disease. Results: Median follow-up time was 42.3 months (range, 8.6-50.8 months). There were no significant differences in the 3-year loco-regional failure-free survival (85.6% vs. 92.3%; p=0.264), distant failure-free survival (87.0% vs. 92.5%; p=0.171), progression-free survival (85.7% vs. 88.4%; p=0.411) or overall survival (86.5% vs. 92.5%, p=0.298) of patients treated concurrently with docetaxel or cisplatin. Severe toxicity was not common in either group. Conclusions: Weekly docetaxel-based concurrent chemoradiotherapy is potentially effective and has a tolerable toxicity; however, further investigations are required to determine if docetaxel is superior to cisplatin for advanced stage NPC.

De novo assembly and transcriptome analysis of differentially expressed genes relevant to variegation in hawthorn flowers

Wei Ji,Wei Zhao,Rong‑Chen Liu,Xiao‑Bo Jiao,Kai Han,Zhong‑Yi Yang,Mei‑Ying Gao,Rui Ren,Xiu‑Juan Fan,Ming‑Xia Yang 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6

Flower color variegation has been observed in many plant species. However, pink flowers on the white-blooming hawthorn trees found by our group earlier have never been reported. To better understand the differentially expressed genes (DEGs) in variegated hawthorn flowers, white and pink flowers at different developmental stages (S1 and S2) underwent transcriptome sequencing separately. Approximately 34.28 Gb of high-quality data were obtained and assembled into 100,013 unigenes with an average length of 706.93 bp. These unigenes were further subjected to functional annotation and biochemical pathway analysis, and DEGs of two types of hawthorn flowers at different developmental stages were studied. Based on the enrichment analysis of DEGs, eight anthocyanin-modified enzyme genes or other enzyme genes that indirectly affect anthocyanin synthesis (5AT, 3GGT , and AI, β-Glu, two Aux/IAAs, two PODs), eight structural genes (UFGT, DFR, CHI, two F3Hs, and three PALs), and three transcription factors (one MYB and two bHLHs) were also identified. We randomly selected 15 genes, and the trends in the expression levels of these genes in the organs of white and pink flowers at different developmental stages were verified by quantitative real-time PCR. Mass sequence data obtained by RNA-seq of variegated hawthorn flowers provided basic sequence information and a unique opportunity to uncover the genetic mechanisms under-lying flower color variegation.

Microporous Zn(II)-MOF for Selective C₂H₂ Adsorption and Anti-cancer Activity on Liver Cancer Combined with LL-37

Xiu-Qin Wei,Shuan-Yuan Yang,Xiao-Xia Li,Mei-Li Xu 한국고분자학회 2021 폴리머 Vol.45 No.6

By employment of a semi-rigid carboxylic acid ligand 5,5" -((5-carboxy-1,3-phenylene)bis(azanediyl))diiso- phthalic acid (H5L) with the -NH- joints, a new porous metal-organic framework (MOF) based on Zn(II) ions as nodes has been generated in success, and its chemical formula is {[Zn₃(μ₃-OH)(L)(H₂O₃)]·3DEF·H₂O}n (1). Owing to its Lewis basic -NH- joints and the π-electron-rich benzene rings on the skeleton, the activated complex 1 offers a favourable pore environment for binding C₂H₂ in preference to CO₂ and CH₄. The inhibitory activity of compound on the liver cancer viability was determined through exploiting the Cell Counting Kit-8 kit. Afterwards, the invasion and migration ability for the liver cancer cells was measured after indicated treatment. Multiple binding interactions are identified by performing the molecular docking simulation, indicating the stabilization effect of the Zn complex towards the target protein, and therefore, the proposed drug molecule exhibits good anticancer activities.

The nonconserved N-terminus of protein phosphatases 1 influences its active site

( Xiu Jie Xie ),( Wei Huang ),( Cheng Zhe Xue ),( Qun Wei ) 한국생화학분자생물학회 (구 한국생화학회) 2008 BMB Reports Vol.41 No.12

Novel Cross-Linked Films Prepared from Hyperbranched Poly(amine-ester)

Wei, Xiu-Zhen,Zhu, Bao-Ku,Xiao, Ling,Xu, You-Yi,Geckeler, Kurt E. Hu@thig & Wepf 2005 Macromolecular Rapid Communications Vol.26 No.15

<P>Summary: Novel hyperbranched poly(amine-ester) (HPAE) cross-linked films were prepared by cross-linking the terminal hydroxyl groups of HPAE using glutaraldehyde (GA). Atom force microscope and scanning electron microscope revealed their smooth surfaces, dense and homogenous matrices. Property characterizations indicated that these cross-linked films had good hydrophilicity, relative low protein adsorption, and high tensile strength. Also, their swelling behavior varied with the solvent.</P><P> <img src='wiley_img/10221336-2005-26-15-MARC200500242-gra001.gif' alt='wiley_img/10221336-2005-26-15-MARC200500242-gra001'> Graphic Structure of the hyperbranched poly(amine-ester). </P>

CCNG2 Suppressor Biological Effects on Thyroid Cancer Cell through Promotion of CDK2 Degradation

Li, Wei-Juan,Liu, Ge-Ling,Yu, Fang,Xiang, Xiu-Xiu,Lu, Yi-Fang,Xiao, Hong-Zhen,Shi, Yan-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

This study aimed to analyze the expression and clinical significance of cyclin G2 (CCNG2) in thyroid carcinoma and the biological effects of CCNG2 overexpression in a cell line. Immunohistochemistry and Western blotting were used to analyze CCNG2 protein expression in 63 cases of thyroid cancer and normal tissues to allow the relationship with clinical factors to be assessed. CCNG2 lentiviral and empty vectors were transfected into the thyroid cancer K1 cell line. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were applied to detect the mRNA and protein levels of CCNG2. MTT assay and cell cycle were also conducted to assess the influence of up-regulated expression of CCNG2 on K1 cell biology. The level of CCNG2 protein expression was found to be significantly lower in thyroid cancer tissue than normal tissues (P<0.05). Western blot: The relative amount of CCNG2 protein in thyroid cancer tissue was respectively found to be significantly lower than in normal tissues (P<0.05), correlating with lymph node metastasis, clinic stage and histological grade (P<0.05), but not gender, age or tumor size (P>0.05). Loss of CCNG2 expression correlated significantly with poor overall survival time on Kaplan-Meier analysis (P<0.05). The results for biological functions showed that K1 cell transfected CCNG2 had a lower survival fraction, a greater percentage in the G0/G1 phases, and lower cyclin-dependent kinase 2 (CDK2) protein expression compared with K1 cells non-transfected with CCNG2 (P<0.05). CCNG2 expression decreased in thyroid cancer and correlated significantly lymph node metastasis, clinic stage, histological grade and poor overall survival, suggesting that CCNG2 may play important roles as a negative regulator in thyroid cancer K1 cells by promoting degradation of CDK2.

Altered mRNA Levels of MOV10, A3G, and IFN-α in Patients with Chronic Hepatitis B

Zhi-Wei Song,Yan-Xiu Ma,Li-Juan Fu,Bao-qing Fu,Xu Teng,Si-Jia Chen,Wei-Zhen Xu,Hong-Xi Gu 한국미생물학회 2014 The journal of microbiology Vol.52 No.6

To explore the relationship of the MOV10, A3G, and IFN-αmRNA levels with chronic hepatitis B virus (HBV) infection,Blood samples from 96 patients with chronic hepatitis B(CHB) and 21 healthy individuals as control were collected. HBV DNA load and aminotransferase in the serum weretested using real time PCR and velocity methods, respectively. The MOV10, A3G, and IFN-α mRNA levels in theperipheral blood mononuclear cells (PBMC) were examinedthrough qRT-PCR. The MOV10, A3G, and IFN-α mRNAlevels in CHB group was significantly lower than those inthe control group (P<0.01, P<0.05, P<0.01, respectively). TheA3G mRNA level in the high-HBV DNA load group waslower than that in the low-HBV DNA load group (P<0.05). However, no statistical difference was found in the MOV10and IFN-α mRNA levels between the two HBV DNA loadgroups. Furthermore, the MOV10 mRNA level showed positivecorrelation with IFN-α in the control group. These resultsindicated that the expression of the innate immune factorsMOV10, A3G, and IFN-α is affected by chronic HBV infection.

스마트폰 아이콘 디자인의 식별성에 관한 연구

위수풍(Wei, Xiu Feng),김희현(Kim, Hee Hyun) 한국디지털디자인협의회 2014 (사)한국디지털디자인협의회 conference Vol.2014 No.5

Today the smart phones are designed as small as possible, and they are designed just according to the visual characteristics of young people. That is not suitable for the olds. The study is based on the visual characteristics of the elderly and the visual elements of the icon(shape,colour,size,motion,text,layout)to make guideline of icon. At first, I investigated which function the elderly use frequently and also which function they want to use. Then I took the button as an object of study, and tried to evaluate the button design based on the visual characteristics of the elderly. I made the survey into two groups, one group has 55 old people who use mobile phones for less than 2 years, and the other one has 10 old people who have experience more than 2 years. Both of them are not satisfied with the design of the size and the layout of icon, So I designed three kind of buttons with different sizes, each one is 0.5time larger than the previous one, the layout has to be changed because of the size of the buttons in the later survey, it turned out the most popular button size is 2 time larger than the icon of the Samsung"s Galaxy S5 is using, and this is the layout of 2*2.

Expression of DOG1, CD117 and PDGFRA in Gastrointestinal Stromal Tumors and Correlations with Clinicopathology

Sun, Xiu-Wei,Feng, Zhan-Jun,Huang, Peng,Hao, Wang,Sui, Xing-Ling Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To discuss the significance of DOG1, CD117 and PDGFRA in the diagnosis of gastrointestinal stromal tumors (GISTs), and analyze their correlations with clinicopathological features and risk ranking. Method: DOG1, CD117 and PDGFRA were detected with IHC Envision ldpe-g-nvp in 63 GISTs and 43 cases of non-GISTs, and analyzed for relations with clinicopathological factors (gender, age, location, tumor size, mitotic phase, histology) and risk degree. Results: The positive expression rate of DOG1, CD117 and PDGFRA in GISTs was 84.1% (53/63), 90.5% (57/63), 53.2% (33/63), respectively. Among the 6 CD117 negative cases, all were DOG1 positive and 5 were PDGFRA positive. Rates in patients with non-GISTs was 11.6%, 16.3%, 6.98%, respectively. Expression of DOG1 and PDGFRA demonstrated no significant variation with gender, age, position, tumor size, mitotic phase, histology, and risk rank. However, CD117 was related with position and histology (P=0.008 and P=0.045), those in the mesentery having a higher positive rate than those derived from stomach, small intestine, colon and rectum (50.0% vs 94.7%, P=0.008). Furthermore CD117 was also highly expressed in spindle and epithele types. Conclusions: DOG1 had a good sensitivity and specificity as a kind of newly discovered marker, especially for KIT negative GISTs. However, DOG1, CD117 and PDGFRA cannot be used for assessing the rish of patients.