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      • KCI우수등재
      • KCI등재

        Characterization of the global transcriptome using Illumina sequencing and novel microsatellite marker information in seashore paspalum

        Xinping Jia,Yanming Deng,Xiaobo Sun,Lijian Liang,Xiaoqing Ye 한국유전학회 2015 Genes & Genomics Vol.37 No.1

        Seashore paspalum (Paspalum vaginatum O. Swartz) is a halophytic warm-season perennial grass,which plays an important role in protection of environment. However, very limited transcriptomic and genomic informationare currently available for seashore paspalum. Inthis study, the seashore paspalum transcriptome weresequenced using Illumina paired-end sequencing technology. In total, 32,603 unigenes with an average length of970 bp were obtained by de novo assembly. Of the unigenes,25,411 unigenes (77.94 %) had significant similaritywith known proteins in the NCBI non-redundant proteinand Swiss-Prot databases. Of these annotated unigenes,20,962 and 10,620 unigenes were assigned to gene ontologycategories and clusters of orthologous groups,respectively. A total of 4,699 unigenes were mapped into118 Kyoto Encyclopedia of Genes and Genomes pathways. In addition, 3,010 potential simple sequence repeats (SSRs)were predicted for microsatellite analyse. Tri-nucleotidewas the dominant repeat (1,583, 52.58 %), followed by dinucleotide(975, 32.39 %) and tetra-nucleotide (217,7.21 %). Fifty SSR sites were randomly selected for validationand development of EST–SSR markers. This studyprovided the global sequence data for seashore paspalumand demonstrated that the Illumina paired-end sequencingis a fast and cost-effective approach to gene discovery andmolecular marker development.

      • KCI등재

        The Effects of Adenoviral Transfection of the Keratinocyte Growth Factor Gene on Epidermal Stem Cells: an In Vitro Study

        Xinping Li,Mingsheng Zhang,Ling Liang,Pin Zhao,Kenzo Uchida,Hisatoshi Baba,Hong Huang,Wenfang Bai,Liming Bai 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

        Epidermal stem cells (ESCs) are characterized as slow-cycling, multi-potent, and self-renewing cells that not only maintain somatic homeostasis but also participate in tissue regeneration and repair. To examine the feasibility of adenoviral vector-mediated keratinocyte growth factor (KGF) gene transfer into in vitro-expanded ESCs, ESCs were isolated from samples of human skin, cultured in vitro, and then transfected with recombinant adenovirus (Ad) carrying the human KGF gene (AdKGF) or green fluorescent protein gene (AdGFP). The effects of KGF gene transfer on cell proliferation, cell cycle arrest, cell surface antigen phenotype, and -catenin expression were investigated. Compared to ESCs transfected with AdGFP, AdKGF-transfected ESCs grew well, maintained a high proliferative capacity in keratinocyte serum-free medium, and expressed high levels of -catenin. AdKGF infection increa-sed the number of ESCs in the G0/G1 phase and promoted ESCs entry into the G2/M phase, but had no effect on cell surface antigen phenotype (CD49f+/CD71-). The results suggest that KGF gene transfer can stimulate ESCs to grow and undergo cell division, which can be applied to enhance cutaneous wound healing.

      • KCI등재

        Adsorption Behaviors and Mechanism of Single/Multi-Metals by Ion Exchange Resins

        Xinping Qian,Shaohui Lin,Xianshe Feng,Garry L. Rempel,Henry Grace,Qinmin Pan 한국고분자학회 2019 폴리머 Vol.43 No.6

        Adsorption of Fe(Ш), Co(II), Cu(II), Zn(II) and K(I) by strong acid resins, Dowex 50WX2 200-400 (H) and Amberlite IRP-69, was studied to investigate the adsorption behaviors and mechanism in single-metal and multi-metal systems. The results showed that 99.9% Fe(III) can be removed by Dowex 50WX2 200-400 (H) in 100 min ([Fe(Ш)] < 40 mg/L, resin amount = 50 mg, pH = 3.0, 60 ºC). Mechanism studies revealed that Langmuir model and pseudo-second- order kinetic equation illustrate better fit for the adsorption of Fe(Ш). Furthermore, the investigations on multi-metal adsorption indicated that resins display a similar adsorption ratio for the same-valence metal ions (Co(II) = Cu(II) = Zn(II)), and a more selective adsorption for high-valence metal ions than low-valence metal ions (Fe(III) > Co(II) > K(I)). The above results can be used for the treatment of wastewater and the recovery of metals from the spent catalysts.

      • KCI등재

        Wind pressure on a solar updraft tower in a simulated stationary thunderstorm downburst

        Xinping Zhou,Fang Wang,Chi Liu 한국풍공학회 2012 Wind and Structures, An International Journal (WAS Vol.15 No.4

        Thunderstorm downbursts are responsible for numerous structural failures around the world. The wind characteristics in thunderstorm downbursts containing vortex rings differ with those in 'traditional' boundary layer winds (BLW). This paper initially performs an unsteady-state simulation of the flow structure in a downburst (modelled as a impinging jet with its diameter being Djet) using a computational fluid dynamics (CFD) method, and then analyses the pressure distribution on a solar updraft tower (SUT) in the downburst. The pressure field shows agreement with other previous studies. An additional pair of low-pressure region and high-pressure region is observed due to a second vortex ring, besides a foregoing pair caused by a primary vortex ring. The evolutions of pressure coefficients at five orientations of two representative heights of the SUT in the downburst with time are investigated. Results show that pressure distribution changes over a wide range when the vortices are close to the SUT. Furthermore, the fluctuations of external static pressure distribution for the SUT case 1 (i.e., radial distance from a location to jet center x=Djet) with height are more intense due to the down striking of the vortex flow compared to those for the SUT case 2 (x=2Djet). The static wind loads at heights z/H higher than 0.3 will be negligible when the vortex ring is far away from the SUT. The inverted wind load cases will occur when vortex is passing through the SUT except on the side faces. This can induce complex dynamic response of the SUT.

      • KCI등재

        The Effect of Gender Imbalance on Housing Price in China

        Xinping HAN,W,N,W,AZMAN-SAINI,Anitha ROSLAND,Yasmin BANI,Siong Hook LAW 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.7

        House ownership is considered as one of the important pre-conditions for marriage in China. Given that gender imbalance is a prominent issue in the country, competition for marriage partners might motivate males to look for a house and probably bigger and more expensive house. This is believed to have caused house price hikes in recent years. This study aims to investigate the impact of gender imbalance on house prices using data from 30 provinces in China for the 2000–2017 period. The results based on the generalized method of moments (GMM) estimations show that house price is strongly influenced by gender imbalance. However, there is no evidence to support differential effects across eastern and mid-western regions. One potential reason is that pre-marriage house ownership has become a common culture for the whole community and therefore it does not vary significantly across regions. There are several important policy implications. Firstly, the issues should be addressed by the policymakers at national level and not regional level. Secondly, the government should intervene to bring back gender ratio to its normal level. Finally, the government should limit the number of houses people can buy and increase the supply of houses in the market.

      • KCI등재

        Numerical Experiments on the Impacts of Surface Evaporation and Fractionation Factors on Stable Isotopes in Precipitation

        Xinping Zhang,Huade Guan,Xinzhu Zhang,Wanjun Zhang,Tianci Yao 한국기상학회 2016 Asia-Pacific Journal of Atmospheric Sciences Vol.52 No.3

        The isotope enabled atmospheric water balance model is applied to examine the spatial and temporal variations of δ18O in precipitation, amount effect and meteoric water lines (MWL) under four scenarios with different fractionation nature and surface evaporation inputs. The experiments are conducted under the same weather forcing in the framework of the water balance and stable water isotope balance. Globally, the spatial patterns of mean δ18O and global MWLs simulated by four simulation tests are in reasonably good agreement with the Global Network of Isotopes in Precipitation observations. The results indicate that the assumptions of equilibrium fractionation for simulating spatial distribution in mean annual δ18O and the global MWL, and kinetic fractionation in simulating δ18O seasonality are acceptable. In Changsha, four simulation tests all reproduce the observed seasonal variations of δ18O in precipitation. Compared with equilibrium fractionation, the depleted degree of stable isotopes in precipitation is enhanced under kinetic fractionation, in company with a decrease of isotopic seasonality and inter-event variability. The alteration of stable isotopes in precipitation caused by the seasonal variation of stable isotopes in vapour evaporated from the surface is opposite between cold and warm seasons. Four simulations all produce the amount effect commonly observed in monsoon areas. Under kinetic fractionation, the slope of simulated amount effect is closer to the observed one than other scenarios. The MWL for warm and humid climate in monsoon areas are well simulated too. The slopes and intercepts of the simulated MWLs decrease under kinetic fractionation.

      • KCI등재

        Influence of nozzle exit tip thickness on the performance and flow field of jet pump

        Xinping LONG,Ning HAN,Qian CHEN 대한기계학회 2008 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.22 No.10

        The influence of exit tip thickness of nozzle δ e on the flow field and performance of a jet pump was studied numerically in this paper. It is found that δ e has influence on the distribution of turbulence kinetic energy k. If δ e is ignored, k takes the highest value but dissipates rapidly than that of nozzle with a certain tip thickness. δ e also affect apparently the development of tip vortex, which will occur near the exit tip of nozzle. The bigger the δ e is, the larger the vortex is. The tip vortex develops with the increase of flow rate ratio q. When q=1 and δ e=0.6~0.8mm, a small vortex will be found downstream the tip vortex. And a concomitant vortex happens down the tip vortex in the case of q=1 and δ e=0.8mm. As q increases to 2, the downstream small vortex disappears and the concomitant vortex becomes bigger. It is also found that the tip vortex might interact with the possible backflow that formed in the throat tube and parts of suction chamber. The center of backflow was affect evidently by δ e. With the increase of δ e, the center of backflow under the same q will go downstream. When δ e=0.4mm, the center of backflow goes farthest. Then, as the further increase of δ e, the center of backflow will go back some distance. Although, δ e has relatively great influence on the flow field within the jet pump, it exerts only a little impact on the performance of jet pump. When δ e=0.2~0.6mm, the jet pump possess better performance. In most case, it is reasonable to ignore the nozzle exit tip thickness in performance prediction for the purpose of simplicity.

      • KCI등재

        Adaptive neural network sliding mode control for serially connected hydraulic cylinders of a heavy-duty hydraulic manipulator

        Xinping Guo,Hengsheng Wang,Liang Wang,Hua Liu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.7

        A sliding mode control based on adaptive neural network is proposed aiming at the automatic control problem of the heavy-duty hydraulic manipulator, which is widely applied in construction machinery. The simplified state space model is established for the two hydraulic cylinders connected in series for the parallel movement of the boom of a rock drilling jumbo manipulator. By using the square of the norm of the neural network weight vector to replace the elements of the weight vector as the adaptive parameter, the computational burden of the controller is reduced and hence becomes more suitable for practical applications. The control law is designed by combining adaptive neural network with sliding mode control, and Lyapunov stability analysis is performed theoretically for the proposed control algorithm. Simulations are conducted to verify the feasibility of the designed controller. Extensive experimental studies are carried out on the heavy-duty hydraulic manipulator of a rock drilling jumbo. When tracking sinusoidal position, the error of the proposed controller is reduced by 53 % and 71 % compared with the traditional sliding mode controller and PID controller, respectively, thereby proving the effectiveness and practicality of the proposed control algorithm.

      • KCI등재

        Study on Applicability of Double Layer Superimposed Primary Support Arch Cover Method for Large-Span Station in Upper Soft and Lower Hard Stratum

        Xinping Guo,Annan Jiang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.3

        The Qingniwaqiao Station from Dalian’s 5th Metro Line in China has exhibited extremely complicated stratum structure, named as the upper soft lower hard (US-LH) stratum. In accordance with the characteristics of the stratum in which the station is located, the special structure form of double layer superimposed primary support arch cover method is presented. Numerical simulations and field monitoring are carried out to study the construction process. Subsequently, the double-layer primary lining, anchor and steel pipe pile are compared and analyzed respectively. The results show that the double-layer primary lining increases the safety reserve when the initial support is removed, and it can beneficially inhibit the deformation of the arch structure resulting in smaller ground surface subsidence. Anchor and steel pipe pile enhance the bearing capacity of rock mass on both sides of the station. The method has advantages in ground surface subsidence control and supporting structure safety. It is safe and feasible to create a large-span subway station using double layer superimposed primary support arch cover method in US-LH relative stratum.

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