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Xiaolong Huang,Huiqing Yan,Yanjing Liu,Yin Yi 한국유전학회 2020 Genes & Genomics Vol.42 No.6
Background LBDs, as the plant-specific gene family, play essential roles in lateral organ development, plant regeneration, as well as abiotic stress and pathogen response. However, the number and characteristic of LBD genes in Pyscomitrella patens were still obscure. Objective This study was performed to identify the LBD family gene in moss and to determine the expression profiles of LBDs under the abiotic and pathogen stress. Methods Complete genome sequences and transcriptomes of P. patens were downloaded from the Ensembl plant database. The hidden Markov model-based profile of the conserved LOB domain was submitted as a query to identify all potential LOB domain sequences with HMMER software. Expression profiles of PpLBDs were obtained based on the GEO public database and qRT-PCR analysis. Results In this study, a total of 31 LBDs were identified in the P. patens genome, divided into two classes based on the presence of the leucine zipper-like coiled-coil motif. A phylogenetic relationship was obtained between 31 proteins from P. patens and 43 proteins from the Arabidopsis thaliana genome, providing insights into their conserved and potential functions. Furthermore, the exon–intron organization of each PpLBD were analyzed. All PpLBD contain the conserved DNA binding motif (CX2CX6CX3C zinc finger-like motif), and were predicted to be located in cell nuclear. The 31 PpLBD genes were unevenly assigned to 18 out of 27 chromosomes based on the physical positions. Among these genes, PpLBD27 was not only remarkably highest expressed in desiccation, but also a susceptible gene to pathogens through jasmonic acid-mediated signaling pathway. Most of PpLBDs were up-regulated with the treatment of mannitol. These results showed they were differentially induced and their potential functions in the environmental stimulus of the early terrestrial colonizers. Conclusion Despite significant differences in the life cycle in P. patens and flowering plants, their functions involved in abiotic and biotic stress-regulated by LBDs have been identified and appear to be conserved in the two lineages. These results provided a comprehensive analysis of PpLBDs and paved insights into studies aimed at a better understanding of PpLBDs.
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Prediction of tectonic fractures in coal reservoirs using geomechanical method
Xiaolong Fu,Junsheng Dai,Jianwei Feng 한국지질과학협의회 2018 Geosciences Journal Vol.22 No.4
Coalbed methane (CBM) resources are one of the most important and broadly developed unconventional resources. Considering the particularity of the coal, an adapted geomechanical method involving elastic-plastic deformation is badly needed to calculate the fracture parameters of the coal reservoir. Based on the rock mechanics experiments and three-dimensional μCT scan, the fracture evolution of coals can be summed up to initial compaction, propagation and coalescence stage. The microfractures will rapidly connect to each other when reaching 85% peak strength. Based on the plenty of measurement data, the relationship between fracture volume density and stress-strain of coals is established, and the acquisition of the quantitative geomechanical models made it possible to characterize fracture parameters after various tectonic movements under different stress state. Furtherly, the preparation of procedural language for the finite element (FE) simulation platform made it more popular and effective in the prediction of the generation of fractures and spatial distribution. The simulated results of No. 3 coal seam of Shizhuangbei area, both of the fracture density and direction, were in agreement with field observation results, which can be a compelling evidence for the effectiveness of the method.
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Xiaolong Yuan,Yunqing L,Ting Luo,Wei Bi,Jiaojun Yu,Yi Wang 한국균학회 2023 Mycobiology Vol.51 No.1
Xanthoria elegans is a lichen symbiosis, that inhabits extreme environments and can absorb UV-B. We reported the de novo sequencing and assembly of X. elegans genome. The whole genome was approximately 44.63 Mb, with a GC content of 40.69%. Genome assembly gen- erated 207 scaffolds with an N50 length of 563,100 bp, N90 length of 122,672 bp. The gen- ome comprised 9,581 genes, some encoded enzymes involved in the secondary metabolism such as terpene, polyketides. To further understand the UV-B absorbing and adaptability to extreme environments mechanisms of X. elegans, we searched the secondary metabolites genes and gene-cluster from the genome using genome-mining and bioinformatics analysis. The results revealed that 7 NR-PKSs, 12 HR-PKSs and 2 hybrid PKS-PKSs from X. elegans were isolated, they belong to Type I PKS (T1PKS) according to the domain architecture; phylogen- etic analysis and BGCs comparison linked the putative products to two NR-PKSs and three HR-PKSs, the putative products of two NR-PKSs were emodin xanthrone (most likely parietin) and mycophelonic acid, the putative products of three HR-PKSs were soppilines, (þ)-asperlin and macrolactone brefeldin A, respectively. 5 PKSs from X. elegans build a correlation between the SMs carbon skeleton and PKS genes based on the domain architecture, phylo- genetic and BGC comparison. Although the function of 16 PKSs remains unclear, the findings emphasize that the genes from X. elegans represent an unexploited source of novel polyke- tide and utilization of lichen gene resources.
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Weak fault detection for wind turbine bearing based on ACYCBD and IESB
Xiaolong Wang,Xiaoli Yan,Yuling He 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.4
To detect the rolling bearing fault of a wind turbine at incipient injury phase, a novel method combining adaptive cyclostationary blind deconvolution (ACYCBD) with instantaneous energy slice bispectrum (IESB) is proposed. Cuckoo search algorithm (CSA) is fused with cyclostationary blind deconvolution (CYCBD) to optimize cyclic frequency and filter length parameters, and the satisfied processing result is able to adaptively use this developed ACYCBD method. Besides, fusing the complementary superiorities of frequency weighted energy operator and slice bispectrum, a new frequency domain analysis method named IESB is put forward to identify the characteristic frequency components. During fault diagnosis, the deconvolution signal with higher signal to noise ratio is first separated from the collected signal by applying ACYCBD method. However, owing to the intense background noises, external interferences still remain in the deconvolution signal. Then the IESB is further used as a post-processing method, the residual noises are suppressed effectively, and the characteristic spectral lines are highlighted immensely. In the end, the incipient fault injury of wind turbine bearing can be diagnosed by analyzing the acquired instantaneous energy slice bispectrum. The analyzed results of wind turbine bearing fault signals from the experimental rig and the actual engineering testing field demonstrate the feasibility and the superiority of this novel detection method.
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Collaborative Innovation in Construction Project: A Social Network Perspective
Xiaolong Xue,Ruixue Zhang,Liang Wang,Hongqin Fan,Rebecca J. Yang,Jason Dai 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.2
Successful innovation requires effective cooperation and working relationships among different parties within construction projects. In order to promote construction innovation performance, it is important to shed light on the internal mechanism of innovation through investigating collaborative relationships from a network perspective. In this case, the formation of collaborative relationship can be viewed as a potential generator of innovation processes, and relationship network indicates information exchanges among organizations. This article investigates the collaborative relationship network in a commercial complex by using social network method and in-depth quantitative data analysis. Structural Equation Modeling (SEM) is usually used to analyze the impacts of collaborative relationship on innovation performance in construction projects. There are more and more stakeholders in construction projects, and organization relationship presents a significant network trend. Social network method is widely applied in innovative research. Combined with quantitative data, it is able to quantify and visual the interaction relations of innovation stakeholder. The analytical results will be more objective and reliable. Social network analysis can describe and analyzed collaborative relationship combining qualitative and quantitative method. The results illustrate the relatively dense collaborative relationship networks and highlight the roles that the key members played in the innovation process. The decomposition of collaborative relationship with network analysis contributes to a better understanding of innovation process in construction projects. In particular, key nodes which influence construction innovation through collaborative relationships are revealed and analyzed.
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The Secure IEEE 11073:20601 Protocol and Agent Structure for Protecting PHD
Xiaolong Xu,Zhonghe Gao,Lijuan Han 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.12
In this paper, we suggest improvements of security in IEEE 11073:20601 protocol and the structure of its agent for protecting user’s healthcare information safely. Current IEEE 11073:20601 standard does not provide any method to ensure security of data exchange, and it assumes that data exchange is secured by other means. The suggested improvements include mechanism to enhance security in exchanging and storing data in PHD. While providing those advantages, it also satisfies availability by effective encoding rules and data protecting algorithm.
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