Molecular cloning and characterization of two novel DREB genes encoding dehydration-responsive element binding proteins in halophyte Suaeda salsa

Xiao-Bo Sun,Hong-Xiang Ma,Xin-Ping Jia,Yu Chen,Xiao-Qing Ye 한국유전학회 2015 Genes & Genomics Vol.37 No.2

The dehydration-responsive element-binding(DREB) proteins play an important role in regulatingexpression of stress-inducible genes under abiotic stresses. In this study, two genes encoding putative DREB proteins,named SsDREBa and SsDREBb, were cloned from halophyteSuaeda salsa L. using RACE method. The deducedSsDREBa and SsDREBb proteins contain a typical AP2/ERF domain. Multiple sequence alignments and phylogeneticanalysis revealed that the two SsDREB genes of S. salsa were highly similar in AP2/ERF domains at thenucleotide and amino acid levels and belong to the A-6subgroup of the DREB transcription factor subfamily. Asubcellular localization assay showed that both SsDREBslocalized to the nucleus. Yeast one-hybrid experimentstestified that both proteins were able to specifically bind tothe DRE sequence and activate the expression of the downstreamHIS reporter gene in yeast. Quantitative real-timePCR analysis demonstrated that under normal conditions,the expression level of SsDREBa was the most high in theroots and no SsDREBa mRNAs were detected in the stems;SsDREBb expressed at relatively higher levels in the leavesthan in the roots and stems. The expression of SsDREa andSsDREBb genes in S. salsa roots and leaves was remarkablyinduced by high-salt and dehydration treatments, butnot by cold and ABA, and exhibited stronger induction inroots and leaves, respectively. These results indicate thatthe SsDREBa and SsDREBb are novel stress-responsivetranscription factors, which are involved in the drought andhigh-salt stress responses through ABA-independent pathwaysand could be used for production of stress-toleranttransgenic crops.

Intrusion Detection System based on Support Vector Machine

Xiao-yun Ye(엽효운),Myung-Mook Han(한명묵) 한국지능시스템학회 2013 한국지능시스템학회 학술발표 논문집 Vol.23 No.1

A Systematic Approach to Improve Fuzzy C-Mean Method based on Genetic Algorithm

Ye, Xiao-Yun,Han, Myung-Mook Korean Institute of Intelligent Systems 2013 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.13 No.3

As computer technology continues to develop, computer networks are now widely used. As a result, there are many new intrusion types appearing and information security is becoming increasingly important. Although there are many kinds of intrusion detection systems deployed to protect our modern networks, we are constantly hearing reports of hackers causing major disruptions. Since existing technologies all have some disadvantages, we utilize algorithms, such as the fuzzy C-means (FCM) and the support vector machine (SVM) algorithms to improve these technologies. Using these two algorithms alone has some disadvantages leading to a low classification accuracy rate. In the case of FCM, self-adaptability is weak, and the algorithm is sensitive to the initial value, vulnerable to the impact of noise and isolated points, and can easily converge to local extrema among other defects. These weaknesses may yield an unsatisfactory detection result with a low detection rate. We use a genetic algorithm (GA) to help resolve these problems. Our experimental results show that the combined GA and FCM algorithm's accuracy rate is approximately 30% higher than that of the standard FCM thereby demonstrating that our approach is substantially more effective.

Functional Roles of Long Non-coding RNA in Human Breast Cancer

Ye, Ni,Wang, Bin,Quan, Zi-Fang,Cao, San-Jie,Wen, Xin-Tian,Huang, Yong,Huang, Xiao-Bo,Wu, Rui,Ma, Xiao-Ping,Yan, Qi-Gui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

The discovery of long noncoding RNA (LncRNA) changes our view of transcriptional and posttranscriptional regulation of gene expression. With application of new research techniques such as high-throughput sequencing, the biological functions of LncRNAs are gradually becoming to be understood. Multiple studies have shown that LncRNAs serve as carcinogenic factors or tumor suppressors in breast cancer with abnormal expression, prompts the question of whether they have potential value in predicting the stages and survival rate of breast cancer patients, and also as therapeutic targets. Focusing on the latest research data, this review mainly summarizes the tumorigenic mechanisms of certain LncRNAs in breast cancer, in order to provide a theoretical basis for finding safer, more effective treatment of breast cancer at the LncRNA molecular level.

Statistical experimental design optimization of rhamsan gum production by Sphingomonas sp. CGMCC 6833

Xiao-Ying Xu,Shu-Hao Dong,Sha Li,Xiao-Ye Chen,Ding Wu,Hong Xu 한국미생물학회 2015 The journal of microbiology Vol.53 No.4

Rhamsan gum is a type of water-soluble exopolysaccharideproduced by species of Sphingomonas bacteria. The optimalfermentation medium for rhamsan gum production bySphingomonas sp. CGMCC 6833 was explored definition. Single-factor experiments indicate that glucose, soybean meal,K2HPO4 and MnSO4 compose the optimal medium alongwith and initial pH 7.5. To discover ideal cultural conditionsfor rhamsan gum production in a shake flask culture, responsesurface methodology was employed, from which thefollowing optimal ratio was derived: 5.38 g/L soybean meal,5.71 g/L K2HPO4 and 0.32 g/L MnSO4. Under ideal fermentationrhamsan gum yield reached 19.58 g/L ± 1.23 g/L,42.09% higher than that of the initial medium (13.78 g/L ±1.38 g/L). Optimizing the fermentation medium results inenhanced rhamsan gum production.

Kinetics Study of Enzymatic Hydrolysis of Paulownia by Dilute Acid, Alkali, and Ultrasonic-assisted Alkali Pretreatments

Xiao-kun Ye,Yuancai Chen 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2

Paulownia, a fast-growing and high-fiber plant (cellulose: 41.66% and hemicellulose: 19.61%), has the potential to serve as an interesting source for production of bioethanol. The aim of this paper is to study and compare the kinetics of enzymatic hydrolysis of Paulownia pretreated by dilute acid (DA), alkali (AL) and ultrasonic-assisted alkali (UA). The enzymatic hydrolysis was performed at 50°C, atmospheric pressure with 40 FPU/g-cellulose cellulase and 80 CBU/g-cellulose cellobiase. The hydrolysis process can be successfully described by the Michaelis-Menten model under the three pretreatment conditions. Due to the high removal of lignin and increased porosity of the substrate, UA pretreatment is proved to be the most effective method in improving enzymatic saccharification, followed by DA pretreatment and alkali (AL) pretreatment. Inhibition constant KI of all experiments (DA: 2.16 g/L, AL: 3.12 g/L and UA: 1.83 g/L) suggests that glucose has a strong inhibition for enzymatic hydrolysis, for lower KI is proportional to higher inhibition performance. The experimental date fits well with kinetics model. This indicates that the model is suitable for performance monitoring, conditions optimization and process control at full-scale studies.

Status and Determinants of Health Literacy among Adolescents in Guangdong, China

Ye, Xiao-Hua,Yang, Yi,Gao, Yan-Hui,Chen, Si-Dong,Xu, Ya Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: Previous studies for non-communicable disease cotrol, including cancer, have mostly relied on health literacy in adults. However, limited studies are available for adolescents. This study aimed to assess the status and determinants of health literacy in in-school adolescents in Guangdong, China. Materials and Methods: A total of 3,821 students aged 13-25 years were selected by multi-stage cluster sampling. After the questionnaire of health literacy was answered, the total scores for health knowledge (18 questions), skills (5 questions) and behaviors (14 questions) were determined. The total scores for health literacy and each subscale were recoded into adequate and inadequate subgroups, and logistic regression models were used to identify factors associated with each outcome variable. Results: The prevalence of adequate health literacy was 14.4%, and the prevalences for adequate knowledge, skills and behavior were 22.4%, 64.7% and 6.6%, respectively. Students coming from prestigious schools and having parents with higher education had higher odds of having adequate knowledge, skills and behaviors. Female students had higher odds of having adequate knowledge and behaviors. Students in grade 7-8 had higher odds of having adequate knowledge and skills. The health knowledge was positive associated with health skills (odds ratio [OR] =2.1, 95% confidence interval [CI] 1.7-2.5) and behaviors (OR=3.0, 95%CI 2.3-4.0), and health skills were positive associated with health behaviors (OR=2.6, 95%CI 1.8-3.8). Conclusions: Further efforts should be made to increase adolescents' health knowledge and behaviors, especially for low grade and male students in non-prestigious schools.

A Study on the Intervention of Property Management Enterprises in Community Governance Model - Based on Property Management of Public Product in Attribute Perspective -

Ye, Zhi-Xiao,Choi, Myeong-Cheol,Kim, Seung-jin The International Promotion Agency of Culture Tech 2020 International Journal of Advanced Culture Technolo Vol.8 No.3

At the end of 2019, there was the major outbreak of "COVID-19" in China. In this outbreak prevention and control campaign, the property management enterprises(PME) performed actively, playing a vital role in China. This paper puts forward that the property management enterprises(PME) should be included as subjects of national urban and rural community governance from the perspective of the public product attribute of property management. Based on the properties of public goods in property management, this paper redefines the functions and positioning of PME with public affairs management, establishes the qualification of PME to participate in one of the main bodies of community public governance. And it forms a new model of community governance with community residents committees, owner' committee and PME as the main body. This research provides implications for national urban and rural community governance models by applying existing theories and practical examples.

A Systematic Approach to Improve Fuzzy C-Mean Method based on Genetic Algorithm

Xiao-Yun Ye,Myung-Mook Han 한국지능시스템학회 2013 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.13 No.3

As computer technology continues to develop, computer networks are now widely used. As a result, there are many new intrusion types appearing and information security is becoming increasingly important. Although there are many kinds of intrusion detection systems deployed to protect our modern networks, we are constantly hearing reports of hackers causing major disruptions. Since existing technologies all have some disadvantages, we utilize algorithms, such as the fuzzy C-means (FCM) and the support vector machine (SVM) algorithms to improve these technologies. Using these two algorithms alone has some disadvantages leading to a low classification accuracy rate. In the case of FCM, self-adaptability is weak, and the algorithm is sensitive to the initial value, vulnerable to the impact of noise and isolated points, and can easily converge to local extrema among other defects. These weaknesses may yield an unsatisfactory detection result with a low detection rate. We use a genetic algorithm (GA) to help resolve these problems. Our experimental results show that the combined GA and FCM algorithm’s accuracy rate is approximately 30% higher than that of the standard FCM thereby demonstrating that our approach is substantially more effective.

siRNA-mediated Silencing of Notch-1 Enhances Docetaxel Induced Mitotic Arrest and Apoptosis in PCa Cells

Ye, Qi-Fa,Zhang, Yi-Chuan,Peng, Xiao-Qing,Long, Zhi,Ming, Ying-Zi,He, Le-Ye Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Purpose: Notch is an important signaling pathway that regulates cell fate, stem cell maintenance and the initiation of differentiation in many tissues. It has been reported that activation of Notch-1 contributes to tumorigenesis. However, whether Notch signaling might have a role in chemoresistance of prostate cancer is unclear. This study aimed to investigate the effects of Notch-1 silencing on the sensitivity of prostate cancer cells to docetaxel treatment. Methods: siRNA against Notch-1 was transfected into PC-3 prostate cancer cells. Proliferation, apoptosis and cell cycle distribution were examined in the presence or absence of docetaxel by MTT and flow cytometry. Expression of $p21^{waf1/cip1}$ and Akt as well as activation of Akt in PC-3 cells were detected by Western blot and Real-time PCR. Results: Silencing of Notch-1 promoted docetaxel induced cell growth inhibition, apoptosis and cell cycle arrest in PC-3 cells. In addition, these effects were associated with increased $p21^{waf1/cip1}$ expression and decreased Akt expression and activation in PC-3 cells. Conclusion: Notch-1 promotes chemoresistance of prostate cancer and could be a potential therapeutic target.