Series D : Construction of Further Education Curricula System for Math Teachers of Senior High Schools

( Xiao Ya He ) 한국수학교육학회 2005 수학교육연구 Vol.9 No.2

Gene-metabolite network analysis in different nonalcoholic fatty liver disease phenotypes

Xiao-Lin Liu,Ya-Nan Ming,Jing-Yi Zhang,Xiao-Yu Chen,Min-De Zeng,Yi-Min Mao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

We sought to identify common key regulators and build a gene-metabolite network in different nonalcoholic fatty liver disease (NAFLD) phenotypes. We used a high-fat diet (HFD), a methionine-choline-deficient diet (MCDD) and streptozocin (STZ) to establish nonalcoholic fatty liver (NAFL), nonalcoholic steatohepatitis (NASH) and NAFL+type 2 diabetes mellitus (T2DM) in rat models, respectively. Transcriptomics and metabolomics analyses were performed in rat livers and serum. A functional network-based regulation model was constructed using Cytoscape with information derived from transcriptomics and metabolomics. The results revealed that 96 genes, 17 liver metabolites and 4 serum metabolites consistently changed in different NAFLD phenotypes (42-fold, Po0.05). Gene-metabolite network analysis identified ccl2 and jun as hubs with the largest connections to other genes, which were mainly involved in tumor necrosis factor, P53, nuclear factor-kappa B, chemokine, peroxisome proliferator activated receptor and Toll-like receptor signaling pathways. The specifically regulated genes and metabolites in different NAFLD phenotypes constructed their own networks, which were mainly involved in the lipid and fatty acid metabolism in HFD models, the inflammatory and immune response in MCDD models, and the AMPK signaling pathway and response to insulin in HFD+STZ models. Our study identified networks showing the general and specific characteristics in different NAFLD phenotypes, complementing the genetic and metabolic features in NAFLD with hepatic and extra-hepatic manifestations.

Relationship Between the SER Treatment Period and Prognosis of Patients with Small Cell Lung Cancer

Xiao, Xiao-Guang,Wang, Shu-Jing,Hu, Li-Ya,Chu, Qian,Wei, Yao,Li, Yang,Mei, Qi,Chen, Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Purpose: To explore the relationship between SER (time between the start of any treatment and the end of radiation therapy) and the survival of patients with limited-stage small cell lung cancer. Materials and Methods: Between 2008 and 2013, 135 cases of limited-stage small cell lung cancer (LS-SCLC) treated with consecutively curative chemoradiotherapy were included in this retrospective analysis. In terms of SER, patients were divided into early radiotherapy group (SER<30 days, n=76) and late radiotherapy group ($SER{\geq}30$ days, n=59) with a cut-off of SER 30 days. Outcomes of the two groups were compared for overall survival. Results: For all analyzable patients, median follow-up time was 23.8 months and median overall survival time was 16.8 months. Although there was no significant differences in distant metastasis free survival between the two groups, patients in early radiotherapy group had a significantly better PFS (p=0.003) and OS (p=0.000). Conclusions: A short SER may be a good prognostic factor for LD-SCLC patients treated with concurrent chemoradiotherapy.

Incorporating Serviceability Limit State Requirements into Reliability-based Analysis and Design of Piles

Xiao-ya Bian,Jun-jie Zheng,Zhi-jun Xu 대한토목학회 2015 KSCE JOURNAL OF CIVIL ENGINEERING Vol.19 No.4

This study develops a novel reliability-based approach for the analysis and design of piles by incorporating Serviceability Limit State (SLS) requirements into Load and Resistance Factor Design (LRFD) for Ultimate Limit States (ULS). Three methods for reliability-based analysis and design, namely the Mean Value First Order Second Moment (MVFOSM) method, the Advanced First Order Second Moment (AFOSM) method, and the Monte Carlo Simulation (MCS) method, are employed for comparison. In order to expediently quantify model uncertainties for SLS and incorporate them into the process of reliability-based analysis and design of piles, the SLS model factor (or bias factor) is established based on a two-parameter, hyperbolic curve-fitting equation describing the load-settlement relation. Herein, the limiting tolerable foundation settlement (slt) is treated as a random variable. Four load test databases from South Africa presented by Dithinde et al. (2011) are compiled again to conduct reliability analysis and calculate resistance factors. The case studies illustrate that the AFOSM method has the higher accuracy than the MVFOSM method as verified using the MCS method. This study recommends the AFOSM method to perform reliability analysis and determine resistance factors for Reliability-Based Design (RBD).

Enhancement of Butanol Tolerance and Butanol Yield in Clostridium acetobutylicum Mutant NT642 Obtained by Nitrogen Ion Beam Implantation

Xiao-Bo Liu,Qiu-Ya Gu,Xiao-Bin Yu,Wei Luo 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyperyield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

QTLs of Cold Tolerance-Related Traits at the Booting Stage for NIL-RILs in Rice Revealed by SSR

Ya Wen Zeng,Shu Ming Yang,Hong Cui,Xiao Juan Yang,Li Ming Xu,Juan Du,Xiao Ying Pu,Zi Chao Li,Zai Quan Cheng,Xing Qi Huang 한국유전학회 2009 Genes & Genomics Vol.31 No.2

QTLs for cold tolerance-related traits at the booting stage using balanced population for 1525 recombinant inbred lines of near-isogenic lines (viz. NIL-RILs for BC5F3 and BC5F4 and BC5F5) over 3 years and two locations by backcrossing the strongly cold-tolerant landrace (Kunmingxiaobaigu) and a cold-sensitive cultivar (Towada) was analyzed. In this study, 676 microsatellite markers were employed to identify QTLs conferring cold tolerance at booting stage. Single marker analysis revealed that 12 markers associated with cold tolerance on chromosome 1, 4 and 5. Using a LOD significance threshold of 3.0, compositive interval mapping based on a mixed linear model revealed eight QTLs for 10 cold tolerance-related traits on chromosomes 1, 4, and 5. They were tentatively designated qCTB-1-1, qCTB-4-1, qCTB-4-2, qCTB-4-3, qCTB-4-4, qCTB-4-5, qCTB-4-6, and qCTB-5-1. The marker intervals of them were narrowed to 0.3-6.8 cM. Genetic distances between the peaks of the QTL and nearest markers varied from 0 to 0.04 cM. We were noticed in some traits associated cold tolerance, such as qCTB-1-1 for 5 traits (plant height, panicle exsertion, spike length, blighted grains per spike and spikelet fertility), qCTB-4-1 for 8 traits (plant height, node length under spike, leaf length, leaf width, spike length, full grains per spike, total grains per spike and spikelet fertility), qCTB-4-2 for 3 traits (spike length, full grains per spike and spikelet fertility), qCTB-5-1 for 5 traits (plant height, panicle exsertion, blighted grains per spike, full grains per spike and spikelet fertility). The variance explained by a single QTL ranged from 0.80 to 16.80%. Three QTLs (qCTB-1-1, qCTB-4-1, qCTB-4-2) were detected in two or more trials. Our study sets a foundation for cloning cold-tolerance genes and provides opportunities to understand the mechanism of cold tolerance at the booting stage.

Pigment Distribution in Fresh Shoot Tissues of Four Colored-leaf Prunus Taxa

Ya Meng Li,Xiao Ling Qu,Xiao Lei Li,Ling Guo,Xiang Shen 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Grain Boundary-Mediated Reduction of Radiation Defects in Different W-Based Alloys

Xiao‑Tong Li,Xiao‑Zhi Tang,Ya‑Fang Guo 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.9

Grain boundaries (GBs) are believed as potent defect sinks that contribute to the radiation damage reduction of materials. The defect-GB interaction has been extensively studied in pure metals, but only a few works shed light on the influence ofmaterial component such as alloying elements. In this study, the GB-mediated reduction of radiation defects in four W-basedalloys (W–Re, W–Ta, W–Mo and W–V) is systematically investigated by atomistic simulations. Quantitative results showthat the defect reduction in W-based alloys is significantly decreased comparing with elementary W. The principal reasonfor this decrease is the presence of various micro configurations where the solute-defect binding interactions are strong. Solutes with high local stress for each atom have a positive effect on the absorption of interstitials by GB. The ability ofGB to absorb interstitials is enhanced with the increasing temperature, and also the accumulation of vacancies is increasedwhen the temperature is as high as 1200 K. The findings in this work provide useful information on the design of radiationtolerance materials.

Reliability Analysis for Serviceability Limit State of Pile Groups Foundation

Xiao-ya Bian,Jun-jie Zheng,Rong-Jun Zhang,Zhi-jun Xu 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.1

The aim of this paper is to present an approach for the assessment of the Serviceability Limit State (SLS) reliability of pile groups foundation combining the Ultimate Limit State (ULS) reliability method of pile groups. To this end, the linear relationship between the ULS and SLS reliability indices is presented using the formulas for calculating the ULS and SLS reliability indices of single piles. Meanwhile, the influence of system effects, correlation coefficient between pile group efficiency and system effects (ρζ,χ), and limiting tolerable settlement (Slt) on the SLS reliability analysis of pile groups foundation is studied using pile load test data compiled for illustration. The results indicate that both the ULS reliability index (βG-uls) and the SLS reliability index (βG-sls) of pile groups foundation decrease with ρζ,χ, that powerful system effects can enhance the ULS and SLS reliability of pile groups foundation, and that βG-sls increases with Slt. the reliability level estimated by pile load test data is equivalent to that incorporating the SLS requirements with a certain value of Slt between 15 mm and 25 mm. For Slt larger than this value, Reliability-based Design (RBD) of pile groups foundation will depend on the ULS requirements, but, conversely, RBD will depend on the SLS requirements.

Metabolomic analysis of biochemical changes in the tissue and urine of proteoglycan-induced spondylitis in mice after treatment with moxibustion

Xiao Xu,Ya-Nan Shi,Rong-Yun Wang,Cai-Yan Ding,Xiao Zhou,Yu-Fei Zhang,Zhi-Ling Sun,Zhi-Qin Sun,Qiu-Hua Sun 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.1

Background: Moxibustion is widely used in East Asian countries to manage the symptom of rheumatic diseases. The aim of this study was to identify potential metabolic profiles of moxibustion on relieving ankylosing spondylitis (AS) mice through UHPLC-Q-TOF/MS metabolomic study. Methods: Thirty-two female Balb/c mice were randomized into healthy control (HC), AS model, moxibustion at acupuncture points (MA) in AS model, and moxibustion at non-acupuncture points (MNA) AS model groups. Moxibustion was administered daily at GV4, bilateral BL23 and bilateral ST36 acupuncture points for four weeks in the MA group. The overall health status, the thickness of hind paws and the tissue concentrations of IL-1β, PGE2, IL-6 and TNF-α were assessed. The UHPLC-Q-TOF/MS was used to explore the perturbations of endogenous metabolites in tissue and urine of AS model mice intervened by moxibustion. Results: Compared with the AS group, the overall health status was significantly improved after 4-week moxibustion intervention (p < 0.05). The results also showed that MA significantly reduced the levels of paw thickness and decreased the levels of four cytokines in the tissue (p < 0.01). Thirty-seven endogenous metabolites identified by the OPLS-DA were considered to be contributing to therapeutic effects of moxibustion. Moreover, metabolic pathway analysis further revealed that the identified metabolites were mainly involved in TCA cycle, Lipid metabolism, Amino Acid metabolism, Intestinal flora metabolism and Purine metabolism. Conclusions: UHPLC-Q-TOF/MS based metabolomics approach, as a novel and powerful tool, can help us to gain the insights into potential mechanisms of action of moxibustion for AS.