Identification of Quantitative Trait Loci (QTLs) for Flowering Time Using SSR Marker in Maize under Water Stress

Xiao, Yan-Nong,Li, Xin-Hai,Zhang, Shi-Huang,Wang, Xiang-Dong,Li, Ming-Shun,Zheng, Yong-Lian 한국유전학회 2004 Genes & Genomics Vol.26 No.4

The selection of reduction of anthesis-silking interval (ASI) in maize breeding is an efficient way to develop maize varieties more tolerant to dry growing conditions. Characterization of the quantitative trait loci (QTL) that controlled the flowering time will be helpful for selection in maize breeding. In this study, flowering time of individuals in a 234 F_(2:3) family, derived from the cross between inbred lines X178 and B73, was evaluated under well-watered and water-stressed conditions at the same location. SSR (microsatellite) was used to identify flowering time QTL. The results showed that the broad-sense heritability for male flowering time (MFT), female flowering time (FFT) and ASI were 0.72, 0.72 - 0.74 and 0.40 - 0.42, respectively, and ASI was significantly correlated to FFT. Under water-stressed condition, 9, 6 and 6 QTLs were identified for MFT, FFT and ASI, respectively, and individual QTL accounted for approximately 2.88% - 31.65% of the phenotypic variation. Some QTLs for MFT were mapped overlapping with those for FIT and ASI. One QTL on chromosome 9 (near nc134) had the strongest effect on MFT, FTT and ASI. It was suggested that the epistasis contributed to the phenotypic variation of flowering time.

Azo dye removal from aqueous solution by organic-inorganic hybrid dodecanoic acid modified layered Mg-Al hydrotalcite

Lanping Nong,Chengliang Xiao,Wei Jiang 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.3

Hydrotalcite (HTC), a typical layered compound, is a promising adsorbent for removal of organic pollutants. To partition azo dye from aqueous solution, Mg-Al HTCs intercalated with dodecanoic acid (DA) modifier, DAHTCs,were prepared by ion exchange and calcination-rehydration methods. The structures of HTCs and DAHTCs were characterized by powder XRD and FT-IR techniques. The introduction of DA broadened the spacing of interlayers and provided more space for ion exchange. The effects of pH value, contact time, adsorbent amount, temperature and different intercalated modifiers on the adsorption of azo dye onto HTCs and DAHTCs were determined. The optimum pH of uptake was around 3.0 and all the lower or higher pH values proved to decrease the adsorption properties. The pseudo-second-order model was found to best describe the adsorption dynamics of all adsorbents. Meanwhile, the size and polarity of intercalated modifiers might be crucial for adsorption of azo dye.

Screening of Anti-Biofilm Compounds from Marine-Derived Fungi and the Effects of Secalonic Acid D on Staphylococcus aureus Biofilm

( Jie Wang ),( Xu-hua Nong ),( Xiao-yong Zhang ),( Xin-ya Xu ),( Muhammad Amin ),( Shu-hua Qi ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.6

Biofilm formation of Staphylococcus aureus is one of its mechanisms of drug resistance. Antibiofilm screening of 106 compounds from marine-derived fungi displayed that 12 compounds inhibited S. aureus biofilm formation by >50% at the concentration of 100 μg/ml, and only secalonic acid D (SAD) and B inhibited by >90% at 6.25 μg/ml without inhibiting cell growth after 24-h incubation. Meanwhile, it was found that the double bond between C-1 and C-10 of citrinin derivatives and the C-C connection position of two chromone monomers may be important for their anti-biofilm activities. Moreover, SAD slightly facilitated biofilm eradication and influenced its architecture. Furthermore, SAD slowed the cell growth rate in the preceding 18-h incubation and differentially regulated transcriptional expression of several genes, such as agr, isaA, icaA, and icaD, associated with biofilm formation in planktonic and biofilm cells, which may be the reason for the anti-biofilm activity of SAD. Finally, SAD acted synergistically against S. aureus growth and biofilm formation with other antibiotics. These findings indicated that various natural products from marine-derived fungi, such as SAD, could be used as a potential biofilm inhibitor against S. aureus.

CAWR: Buffer Replacement with Channel-Aware Write Reordering Mechanism for SSDs

Ronghui Wang,Zhiguang Chen,Nong Xiao,Minxuan Zhang,Weihua Dong 한국전자통신연구원 2015 ETRI Journal Vol.37 No.1

A typical solid-state drive contains several independent channels that can be operated in parallel. To exploit this channel-level parallelism, a variety of works proposed to split consecutive write sequences into small segments and schedule them to different channels. This scheme exploits the parallelism but breaks the spatial locality of write traffic; thus, it is able to significantly degrade the efficiency of garbage collection. This paper proposes a channel-aware write reordering (CAWR) mechanism to schedule write requests to different channels more intelligently. The novel mechanism encapsulates correlated pages into a cluster beforehand. All pages belonging to a cluster are scheduled to the same channels to exploit spatial locality, while different clusters are scheduled to different channels to exploit the parallelism. As CAWR covers both garbage collection and I/O performance, it outperforms existing schemes significantly. Trace-driven simulation results demonstrate that the CAWR mechanism reduces the average response time by 26% on average and decreases the valid page copies by 10% on average, while achieving a similar hit ratio to that of existing mechanisms.

Analysis of Small Fragment Deletions of the APC gene in Chinese Patients with Familial Adenomatous Polyposis, a Precancerous Condition

Chen, Qing-Wei,Zhang, Xiao-Mei,Zhou, Jian-Nong,Zhou, Xin,Ma, Guo-Jian,Zhu, Ming,Zhang, Yuan-Ying,Yu, Jun,Feng, Ji-Feng,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

Research on Modeling Improvement of Rubber Isolators by Considering Multi-loading Conditions

Yin-Yan Zhao,Wen-Bin Shangguan,Nong Cheng,Xiao-Jing He 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

The stress-strain relationships of a rubber material in uniaxial tension, equi-biaxial tension and plane shear loading conditions are tested here. Constitutive parameters in different constitutive models, such as Mooney-Rivlin model, Van Der Waals model and Marlow model can be obtained by least square method using measured and calculated stress-strain relations. The rubber isolators in two rubber mounts are investigated here. Rubber mount I bears stretching/compression deformation or shear deformation, while mount II bears both deformations simultaneously. Using obtained constitutive constants, the rubber isolators’ static stiffness values in three mutually perpendicular directions are calculated, analyzed and compared with their measured results. Based on Mooney-Rivlin model, the effects of these constitutive constants on static stiffness of the two mounts are discussed by comparison. The effects of different models on static force-displacement characteristics of rubber mount I are also analyzed. The conclusion is that it is necessary to choose a relatively accurate constitutive model and appropriate methods for obtaining the model constants in describing rubber characteristic.

Preparation and High Degradation Activity of Supported Nano-Bi2WO6-TiO2/Nickel Foam Photocatalyst

Qin Tong,Ya-Mei Dong,Xiao-Jia Wang,Peng Yan,Dan-Nong He 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.6

The supported nano-Bi2WO6–TiO2/nickel foam photocatalyst was synthesized via spraying method with silica sol as a binder. The as-prepared photocatalyst with large surface and thermal stability exhibited enhanced photocatalytic performance for degradation of Rhodamine B (RhB) solution in sunlight irradiation. The results showed that the RhB solution could be degraded to 71.6% within 60 min, exhibiting that improved photocatalytic activity increased by 77.6%, compared with that of pure Bi2WO6–TiO2 nanoparticles. Its high photocatalytic activity was due to the presence of nickel foam in the catalytic process, making the Bi2WO6–TiO2 highly dispersed and increasing the contact area of the photocatalysts with the organic pollutants. At the same time, a possible photocatalytic mechanism for the enhanced photocatalytic performance was investigated.

Aberrant Expression of Markers of Cancer Stem Cells in Gastric Adenocarcinoma and their Relationship to Vasculogenic Mimicry

Zhou, Lei,Yu, Lan,Feng, Zhen-Zhong,Gong, Xiao-Meng,Cheng, Ze-Nong,Yao, Nan,Wang, Dan-Na,Wu, Shi-Wu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Background: Gastric cancer is the second leading cause of cancer-related death in Asia, and the majority type is gastric adenocarcinoma (GAC). Most GAC patients die of recurrence and metastasis. Cancer stem cells (CSCs) have been thought to be responsible for the initiation, development, metastasis, and ultimately recurrence of cancer. In this study, we aimed to investigate expression and clinical significance of CSCs markers, CD133 and Lgr5, and vasculogenic mimicry (VM) in primary GAC. Materials and Methods: Specimens from 261 Chinese patients with follow-up were analyzed for CD133, Lgr5 protein expression and VM by immunohistochemical and histochemical staining. The Pearson Chi's square test was used to assess the associations among the positive staining of these markers and clinicopathological characteristics. Postoperative overall survival time was were studied by univariate and multivariate analyses. Results: In GAC tissues, positive rates of 49.0%, 38.7%, and 26.8% were obtained for CD133, Lgr5, and VM, respectively. The mean score of microvessel density (MVD) was $21.7{\pm}11.1$ in GAC tissues. There was a significantly difference between the positive and negative groups. There was a positive relationship between the VM, the expression of CD133 and Lgr5, and the score of MVD and the grades of tumor, lymph node metastasis, TNM stages (all p<0.05). The overall mean survival time of the patients with CD133, Lgr5, VM, and MVD (${\geq}22$) positive expression was lower than that of patients with negative expression. The score of MVD, positive expression of CD133 and VM were independent prognostic factors of GAC (p<0.05). Conclusions: VM, and expression of CD133, Lgr5, and the score of MVD are related to grades of tumor, lymph node metastasis, TNM stages, and overall mean survival time. It is suggested that CSCs and VM could play an important role in the evolution of GAC.

Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis

Qing-Li Yang,Ji-Qing Shen,Yan Xue,Xiao-Bing Cheng,Zhi-Hua Jiang,Yi-Chao Yang,Ying-Dan Chen,Xiao-Nong Zhou 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6

The nitric oxide (NO) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. We still do not know much about the responses of inducible NO synthase (iNOS) induced by Clonorchis sinensis infection. This study was conducted to explore the pathological lesions and iNOS expressions in the liver of mice with different infection intensity levels of C. sinensis. Extensive periductal inflammatory cell infiltration, bile duct hyperplasia, and fibrosis were commonly observed during the infection. The different pathological responses in liver tissues strongly correlated with the infection intensity of C. sinensis. Massive acute spotty necrosis occurred in the liver parenchyma after a severe infection. The iNOS activity in liver tissues increased, and iNOS-expressing cells with morphological differences were observed after a moderate or severe infection. The iNOS-expressing cells in liver tissues had multiple origins.

Growth and Differentiation Effects of Homer3 on a Leukemia Cell Line

Li, Zheng,Qiu, Hui-Ying,Jiao, Yang,Cen, Jian-Nong,Fu, Chun-Mei,Hu, Shao-Yan,Zhu, Ming-Qing,Wu, De-Pei,Qi, Xiao-Fei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

The Homer protein family, also known as the family of cytoplasmic scaffolding proteins, which include three subtypes (Homer1, Homer2, Homer3). Homer3 can regulate transcription and play a very important role in the differentiation and development for some tissues (e.g. muscle and nervous systems). The current studies showed that Homer3 abnormal expression changes in acute myeloid leukemia (AML). Forced expression of Homer3 in transfected K562 cells inhibited proliferation, influenced the cell cycle profile, affected apoptosis induced by $As_2O_3$ through inhibition of Bcl2 expression, and also promoted cell differentiation induced by 12-O-tetra decanoylphorbol-acetate (TPA). These results showed that Homer3 is a novel gene which plays a certain role in the occurrence and development of AML.