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趙成九,崔香順,朴相一 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-
Diets containing 0.0%, 0.2%, 0.5%, and 1.0% of SLPBFL were fed to broiler chickens at 7 weeks age. The results of SLPBFL feeding are as follows; Body weight gain was significantly improved in 0.5% SLPBFL diet group ( p < 0.01 ). Because feed consumption was increased with adding of SLPBFL, broilers taste was not considered in this experiment. Feed requirement was analyzed same score to 1.99 in 0.2% and 0.5% SLPBFL added rations be improved about 8% than the control group. The amounts of carcass, drumsticks and breast meat were significantly(p<0.05) plenty in 0.5% treatment, and also the carcass ratio was enhanced to the highest score in 0.5% SLPBFL, In 0.5% treatment, birds were improved of growth rate, the head and neck weights, leg and shank weight, and amounts of blood and feather were higher appeared than other treatments. The weight of internal organs (liver, heart, spleen, gizzard) were appeared to be heavy in 0.5% treatment. The fat accumulation (abdominal and gizzard surrounding) was observed from the broiler chickens fed 0.5%, tended to increase with fat contents agreeably to live weight gain (P<0.05). Total serum protein, serum bilirubin and LDL-cholesterol concentrations in non- SLPBFL were lower than those in SLPBFL treatments. The concentrations of serum GPT, serum GOT, serum albumin and HDL- cholesterol in non-SLPBFL bird were the lowest in treatment. The concentrations of serum HDL-cholesterol and albumin in 0.5% treatment to be were appeared to be highest score and the concentration HDL-cholesterol was analyzed the lowest score, and total serum protein, serum GPT, serum GOT, serum bilirubin, total serum cholesterol, HDL-cholesterol and serum triglyceride concentrations were analyzed to be mean score in experimental diets. Key words : Bupleurum falcatum Linne, serum protein, serum GPT, serum GOT, serum Bilirubin, total serum cholesterol, HDL-cholesterol, serum triglyceride.
趙成九,崔香順,朴相一 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-
Studies on the utilization added in diet with a Stem and Leaf Powder of Angelicae Gigas Nakai(SLPAGN) in the broiler chickens. Diets added with 0.0%, 0.2%, 0.5%, and 1.0% of SLPAGN were fed to broiler chickens at 7 weeks age. The results of SLPAGN feeding are as follows; The body weight gain was significantly improved in 1.0% SLPAGN diet (P<0.01), and feed efficiency was effected with 1.0% SLPAGN ration(P<0.05). The amount of carcass was the heaviest in 0.2% SLPAGN diet. The amounts of breast meat and drumsticks product were the highest in 0.2% SLPAGN, and the weights of head and neck, leg and shank were lower than other treatments. The fat accumulation (abdominal and gizzard surrounding) was observed in broilers fed diets added with 0.2% SLPAGN. The weights of liver, spleen and gizzard were tended to increase with adding SLPAGN rations than control diet. The degradation of fabricius sac was delayed to non-SLPAGN treatment than SLPAGN groups. The blood contents were analyzed to be the highest level to the total serum, GOT, total serum cholesterol and LDL-cholesterol concentrations in the broiler chickens fed 1.0% SLPAGN diets, and then the level of total serum protein and HDL-cholesterol concentrations were appeared to be the lowest. In the birds fed non-SLPAGN treatments the concentration of serum GPT, serum albumin, total serum triglyceride, total serum cholesterol and LDL-cholesterol were analyzed to be the lowest levels. In 0.2% SLPAGN treatment, the contents of serum GOT and serum bilirubin contents were analyzed the lowest levels. Key words: Angelicae Gigas, feed efficiency. HDL-cholesterol. Carcass ratio, serum bilirubin, GPT, serum albumin, serum triglyceride, serum albumin, GOT
The Role of microRNAs on the Epigenetic Regulation of Fertilized and Cloned Embryo Development
Xiang-Shun Cui,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Cloning or somatic cell nuclear transfer (SCNT) using adult somatic cell to derive cloned embryos is a promising new technology with potential applications in both agriculture and regenerative medicine. Mammalian embryos derived by nuclear transfer are capable of development to the blastocyst stage with a relatively high efficiency of 30~ 50%. However, in full-time development, usually only 2% of NT embryos can result in live births due to abnormalities in placenta formation. In SCNT embryos, the donor cell nucleus is epigenetically reprogrammed by oocyte cytoplasm during development. Incomplete reprogramming of the donor cell genome is considered a major reason for low cloning efficiency. Aberrant epigenetic modifications include DNA methylation, histone modification and X-chromosome-inactivation. Due to a lack of basic knowledge regarding the embryos following nuclear transfer, the success rate of cloning is low. Therefore, elucidation of the molecular mechanism of SCNT embryo development will be of great value for further research. MicroRNAs (microRNA) are single-strand RNA molecules of about 19 23 nucleotides in length, which regulate gene expression by imperfect base pairing with target mRNA, subsequently guiding mRNA cleavage or translational repression. Since the first discovery and functional annotation in 1993 of the small RNA, lin-4 and let-7, which are involved in developmental timing and gene regulation during C. elegans larval development, microRNAs have received scientific attention. Now hundreds of microRNAs have been identified in various multicellular organisms, and many microRNAs have been shown to be evolutionarily conserved. The roles proposed for this novel class of tiny RNA molecules are diverse. They are likely to be involved in developmental timing, differentiation, cell proliferation, signaling pathways, apoptosis, metabolism, heterochromatin formation, genome rearrangement, brain development and carcinogenesis. Currently (2006- present) we are working to determine the role of microRNAs on the epigenetic regulation of fertilized and cloned embryo development. The general hypothesis of our research is that genetic and epigenetic factors regulate the development of preimplantation mammalian embryos, and aberrant modulations in cloned embryos are causes of abnormal development and low success rate of cloned embryos.
Cui, Xiang-Shun,Li, Xing-Yu,Shen, Xing-Hui,Bae, Yong-Ju,Kang, Jason-Jongho,Kim, Nam-Hyung JOHN WILEY & SONS LTD 2007 MOLECULAR REPRODUCTION AND DEVELOPMENT Vol.74 No.2
<P>To gain insight into early embryo development, we utilized microarray technology to compare gene expression profiles in four-cell (4C), morula (MO), and blastocyst (BL) stage embryos. Differences in spot intensities were normalized, and grouped by using Avadis Prophetic software platform (version 3.3, Strand Genomics Ltd.) and categories were based on the PANTHER and gene ontology (GO) classification system. This technique identified 622 of 7,927 genes as being more highly expressed in MO when compared to 4C (P < 0.05); similarly, we identified 654 of 9,299 genes as being more highly expressed in BL than in MO (P < 0.05). Upregulation of genes for cytoskeletal, cell adhesion, and cell junction proteins were identified in the MO as compared to the 4C stage embryos, this means they could be involved in the cell compaction necessary for the development to the MO. Genes thought to be involved in ion channels, membrane traffic, transfer/carrier proteins, and lipid metabolism were also identified as being expressed at a higher level in the BL stage embryos than in the MO. Real-time RT-PCR was performed to confirm differential expression of selected genes. The identification of the genes being expressed in here will provide insight into the complex gene regulatory networks effecting compaction and blastocoel formation. Mol. Reprod. Dev. © 2006 Wiley-Liss, Inc.</P>
Gene Expression of Cox5a, 5b, or 6b1 and Their Roles inPreimplantation Mouse Embryos1
Cui, Xiang-Shun,Li, Xing-Yu,Jeong, Yu-Jeong,Jun, Jin-Hyun,Kim,Nam-Hyung Society for the Study of Reproduction 2006 BIOLOGY OF REPRODUCTION Vol.74 No.3
To investigate the role of nuclear encoded genes in mitochondrialfunction during oocyte maturation and early embryogenesis weexamined the expression pattern and function of the cytochromeoxidase (Cox) subunits, Cox5a, 5b, and 6b1 during oocytematuration and early embryo development. Transcription of Cox5a,5b, or 6b1 was observed in oocytes and during early development;their expression levels were abundant in mature oocytes (MII) andzygotes (1C), and lowest at the 2-cell stage (2C), graduallyincreasing from 4-cell to blastocyst stage. Immunocytochemicalstudies revealed that COX5A, 5B, or 6B1 proteins were expressed inall blastomeres of the blastocyst. Silencing of mRNA expression byRNA interference (siRNA) did not inhibit oocyte maturation ordevelopmental events up to the morula and blastocyst stages, butdisrupted mitochondrial distribution. Significantly higherapoptosis and lower cell numbers were observed in siRNA-treatedblastocysts. Real time RT-PCR revealed that silencing of Cox5a,5b, or 6b1 did not alter mRNA levels of Bcl-xL (Bcl2l1), butincreased transcription levels of proapoptotic genes, Bax andcaspase 3 (Casp3). Furthermore, mRNA and protein levels ofE-cadherin (CDH1) were decreased in siRNA microinjectedblastocysts. These results suggest that gene expression of the Coxsubunits, Cox5a, 5b, and 6b1 is not required for embryodevelopmental events up to the blastocyst stage. The loss of thesegenes leads to mitochondrial dysfunction that results in apoptosisof the blastocyst stage embryos.