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Byeong-chunLee,Kwang-teakLim,Sung-keunKang,Woo-sukHwang 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.1
In this study,we examined the effects of a two-stepculture system, which involves the use of differentculture media for early cleavage and later stageembryos, on the in vitro development of bovineembryos. We also investigated the effect of glucose,phosphate and citrate on the in vitro early develop-mental period of bovine embryos in a two-stepculture system. Moreover, the supplementation ofdifferent protein sources (BSA-V, BSA-FAF and FBS)during IVC did not affect the frequency of blastocystdevelopment. Using two-step culture, embryos werecultured in protein-free media for an initial 5 days.This was then followed by the same culture media oran FBS supplemented media. The developmentalrates of blastocysts in the FBS containing group weresignificantly higher than in the replaced with noserum containing group. Embryos cultured in mSOFsupplemented with 1.5 mM glucose plus 1.2 mMphosphate were significantly inhibited.The inhibitionof developmental competence by glucose plus phos-phate was consistent with the existence of 0.5 mMsodium citrate. This study indicates that a two-stepculture system,which applies different conditions forearly cleavage embryos, i.e., serum-free media, vs.later stage embryos, with serum containing media,may be effective for in vitro production systems. Inaddition, the developmental competence of bovineembryos was depressed in the presence of glucoseplus phosphate as compared to either alone or theabsence of both. Therefore, the avoidance of thisnegative effect should allow more optimal conditionsto be developed for in vitro production.*Corresponding author: Byeong-chun Lee
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