Extremely sensitive and selective sub-ppm CO detection by the synergistic effect of Au nanoparticles and core–shell nanowires

Kim, Jae-Hun,Mirzaei, Ali,Kim, Hyoun Woo,Kim, Sang Sub Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.249 No.-

<P><B>Abstract</B></P> <P>An extremely sensitive CO sensor based on novel hybrid SnO<SUB>2</SUB>–ZnO core–shell (C–S) nanowires (NWs) functionalized by Au nanoparticles (NPs) was synthesized. First, networked SnO<SUB>2</SUB> NWs were successfully prepared using the vapor–liquid–solid growth method on an electrode layer with special patterns. A ZnO shell was subsequently coated on the SnO<SUB>2</SUB> core using atomic layer deposition, and Au NPs were then attached onto the ZnO shell using γ-ray radiolysis. The resulting sensor exhibited a very high response of 26.6–100ppb CO gas. Furthermore, the responses to other gases such as C<SUB>6</SUB>H<SUB>6</SUB> and C<SUB>6</SUB>H<SUB>7</SUB> were extremely low, indicating the very good selectivity of the sensor to CO gas. Besides acting as heterojunctions, the catalytic effect of the Au NPs on CO gas greatly improved the CO sensing capability of the Au-functionalized SnO<SUB>2</SUB>–ZnO C–S NWs. The high sensitivity and selectivity of this sensor can open a path for prompt toxic monitoring and early detection of fatal diseases related to CO.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel hybrid SnO<SUB>2</SUB>–ZnO core–shell nanowires functionalized by Au nanoparticles. </LI> <LI> The resulting sensor exhibited a very high response of 26.6–100ppb CO gas. </LI> <LI> The very good selectivity of the sensor to CO gas. </LI> <LI> Heterojunctions and the catalytic effect of Au greatly improved the CO sensing capability. </LI> </UL> </P>

A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

CPT column을 이용한 석탄 품위향상 기술 연구

김우람 ( Woo Ram Kim ),이윤섭 ( Yun Sub Lee ),한오형 ( Oh Hyung Han ) 조선대학교 공학기술연구원 2015 공학기술논문지 Vol.8 No.2

Development of commercial technology for expansion in domestic anthracite coal utilization is urgent due to the mass reserves of low grade anthracite in Korea. Therefore, a series of experiments were conducted in this study to resolve this problem using CPT column in order to check the possibility of high-quality production for the fine coal that is impossible to treat by dry separation method. Particle size analysis was conducted for the sample using disk mill and vibro screen, and preliminary experiment was conducted for +100 mesh of the product obtained through this process by batch flotation(Sub-A type). Comparison experiment was conducted for -100 mesh and 100 × 325 mesh by each factor-specific condition applying to CPT Column. The result of CPT column flotation by using the proper reagent confirmed through batch flotation was ash rejection 83.15% and recovery 91.44% under the conditions of pulp density 5%, frother temperature 26°C, Kerosene collector (200mL/ton), Pine oil frother (1L./ton), Lime depressant (3kg/ton), air flow rate (1,400mL/ton) and wash water (300mL/ton).

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

우취(牛膵) Ribonuclease가 자궁암 세포의 염색체수에 미치는 영향에 관한 연구

이석우,박혜숙,장남섭 최신의학사 1970 最新醫學 Vol.13 No.7

Chroniosome numbers have been studied in a Hela cell population sensitive to the inhibitory influences of RNase(strain S3) and in its sub-lines-,r resistant to RNase according to the length of time and the density of concentration, respectively. It has been found that strain S3 has a stem-line of 59 chromosomes, while the RNase-resistant sub lines of exhibit stem-lines of 56,53 and 50. The data suggest that RNase "resistancy" in the cell strain and sub-lines is due to subler varj4tion than a net decrease of 3,6 and I9 chromosomes, respectively.

바른체형운동 지도가 정신장애인의 우울감 및 폐활량에 미치는 효과

우경희(Kyung-Hee Woo),양정옥(Jeong-Ok Yang),이중숙(Joong-Sook Lee),이범진(Bom-Jin Lee),김의숙(Eui-Suk Kim),정숙희(Sook-Hee Jung),곽이섭(Yi-Sub Kwak) 한국생명과학회 2016 생명과학회지 Vol.26 No.10

본 연구는 바른체형운동 프로그램이 정신장애인의 폐활량 및 우울감에 미치는 영향을 알아보고자 경북 P시에 거주하고 정신장애 판정을 받았으며 정신재활프로그램에 참여하는 정신장애인을 대상으로 실시하였다. 참여한 피험자에게 연구목적과 내용을 설명하였고, 연구 참가동의서를 받은 19명을 최종 선정하여 10명은 운동군, 9명은 통제군으로 분류하여 12주간 바른체형운동 프로그램을 실시하였고, 연구결과는 다음과 같다. 우선 폐활량의 변화를 살펴본 결과 PEF (최대호기속도)와 FEV1/FVC (1초간 노력성 호기량의 노력성 폐활량에 대한 비)가 바른체형운동 적용 후에 증가하여 호기기능이 유의하게 향상됨을 확인하였다. 또한, 바른체형운동 프로그램 참여에 따른 운동군의 우울감의 변화는 통계적으로 유의한 차이는 나타나지 않았으나 우울정도의 감소폭이 통제군보다 운동군에서 훨씬 크게 나타나 바른체형운동이 정신장애인의 우울감 정도의 감소에 긍정적인 변화를 나타냈다고 할 수 있다. 그리고 본 연구에서는 프로그램 참가자들의 특수성으로 인한 표본 수의 한계로 인해 통계적 검증의 한계가 명확히 존재함을 고려하여 참여자들의 주관적 경험을 심층적으로 이해하는 것을 목적으로 한 질적분석(qualitative research)을 병행하여 실시하였다. 프로그램 참가자 중 동의하는 사람에 한해 심층적 면담을 실시하였으며 이를 통해 참여자들이 표현하는 내용의 의미단위를 검토하여 분류하고 세분화하는 작업을 통해 주제분석(thematic analysis)을 실시한 결과, 전반적으로 바른체형운동 프로그램에 참여한 정신장애인들의 우울감 정도가 줄어든 것을 알 수 있었다. 우울감의 변화과정은 ‘변화의 시작’, ‘프로그램에 대한 흥미’, ‘신체적 건강의 필요성에 대한 인식’, ’신체적 건강증진’, ’신체적 기능의 회복’, ’부정적인 습관(자세)의 변화’, ’우울감의 감소’, ’할 수 있다는 자신감’, ’미래에 대한 희망’과 같은 3개의 주제와 6개의 하위 주제로 나타났다. 따라서 바른체형운동이 정신장애인들의 신체적 건강증진을 위해 심폐지구력을 높여 신체적 건강증진을 도모할 뿐 아니라 더 나아가 정신적 우울감의 감소에도 효과성을 높여 정신적 건강증진에도 도움을 줄 수 있는 프로그램으로 정신장애인의 삶의 질 증진을 위해서는 보급이 반드시 필요할 것으로 사료된다. The purpose of this study was to investigate the effects of the regular Upright Body Type Excercise on posture, muscle strength of leg, lung capacity and depression of people with mental illness. The subjects of this study were recruited from B mental health center (5 years and more mental illness patients, n=19) located in P city. For the exercise group, They were carried out the Upright Body Type Excercise Program during 8 weeks (60 min/time, 3 times/week). They were randomly divided into two groups. Exercise group (n=10) and Control group (n=9). And also, they were agreed with consent forms before the experiments. Research results were as follows. Through the upper body type exercise, there was significantly difference in PEF and FEV1/FVC in the trained group. And also, there was much improved in depression level in the trained group. The change of melancholy feeling before and after the program was not statistically significant. However, because of limitations of sampling size due to the peculiarities of the program participants, in consideration of the limit of statistical validation exists clearly, intended to better understand the subjective experience of attendees, qualitative analyzing(qualitative research) was carried out in parallel. It was conducted a deep interview only person accepted among program participants, thematic analysis, subject analysis tasks to be subdivided by classifying by considering the semantic units of what participants expressed, was thus carried out. It found that the degree of melancholy feeling of mental disorders who participated in the Upright Body Typed Exercise Program was reduced. The course of the experience of change in depression appered the three subjects and six sub-themes such as “the start of the change”, “interest of the program”, “recognition of the need of the body`s health”, “physical health promotion”, “recovery of physical function”, “negative change of habits (attitude)”, “reduction of sense of depression”, “confidence that it is possible to”, “hope for the future”. Therefore, upper body type exercise is much helpful in lung capacity and mental health of people with mental illness. So, this type of exercise mostly needed in the people with mental illness group than the other group for the quality of life.

A 12-b Asynchronous SAR Type ADC for Bio Signal Detection

Lim, Shin-Il,Kim, Jin Woo,Yoon, Kwang-Sub,Lee, Sangmin The Institute of Electronics and Information Engin 2013 Journal of semiconductor technology and science Vol.13 No.2

This paper describes a low power asynchronous successive approximation register (SAR) type 12b analog-to-digital converter (ADC) for biomedical applications in a 0.35 ${\mu}m$ CMOS technology. The digital-to-analog converter (DAC) uses a capacitive split-arrays consisting of 6-b main array, an attenuation capacitor C and a 5-b sub array for low power consumption and small die area. Moreover, splitting the MSB capacitor into sub-capacitors and an asynchronous SAR reduce power consumption. The measurement results show that the proposed ADC achieved the SNDR of 68.32 dB, the SFDR of 79 dB, and the ENOB (effective number of bits) of 11.05 bits. The measured INL and DNL were 1.9LSB and 1.5LSB, respectively. The power consumption including all the digital circuits is 6.7 ${\mu}W$ at the sampling frequency of 100 KHz under 3.3 V supply voltage and the FoM (figure of merit) is 49 fJ/conversion-step.

Haplogroup Classification of Korean Cattle Breeds Based on Sequence Variations of mtDNA Control Region

Kim, Jae-Hwan,Lee, Seong-Su,Kim, Seung Chang,Choi, Seong-Bok,Kim, Su-Hyun,Lee, Chang Woo,Jung, Kyoung-Sub,Kim, Eun Sung,Choi, Young-Sun,Kim, Sung-Bok,Kim, Woo Hyun,Cho, Chang-Yeon Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

Many studies have reported the frequency and distribution of haplogroups among various cattle breeds for verification of their origins and genetic diversity. In this study, 318 complete sequences of the mtDNA control region from four Korean cattle breeds were used for haplogroup classification. 71 polymorphic sites and 66 haplotypes were found in these sequences. Consistent with the genetic patterns in previous reports, four haplogroups (T1, T2, T3, and T4) were identified in Korean cattle breeds. In addition, T1a, T3a, and T3b sub-haplogroups were classified. In the phylogenetic tree, each haplogroup formed an independent cluster. The frequencies of T3, T4, T1 (containing T1a), and T2 were 66%, 16%, 10%, and 8%, respectively. Especially, the T1 haplogroup contained only one haplotype and a sample. All four haplogroups were found in Chikso, Jeju black and Hanwoo. However, only the T3 and T4 haplogroups appeared in Heugu, and most Chikso populations showed a partial of four haplogroups. These results will be useful for stable conservation and efficient management of Korean cattle breeds.

스테로이드로 유발된 쥐의 근골격계 변화에대한 성장호르몬의 치료 효과

최기섭,박창일,정우성,이상건,박용욱 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.5

마이크로-매크로 셀룰라 시스템을 위한 계층선정 기법분석

김장섭,이상헌,정우곤,최형진 成均館大學校 科學技術硏究所 1998 論文集 Vol.49 No.1

A control scheme to select the appropriate layer between macro- and microcell hierarchical structure is considered. With the assumption that the each mobile speed is known to the system. the algorithm determines the optimal velocity threshold by which the cell section performed. The optimal criterion is to minimize the Grade of Service(GOS), a service criterion in cellular telephone systems. Mobile station with faster speed relative to the determined optimal speed threshold are assigned to a macrocell, while slower moving terminals are assigned to the smaller cells, i,e.. microcells. For the evaluation purpose for the proposed scheme, two different evaluation perspectives are compared and the algorithm proves to work