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      • Bifidobacterium angulatum KCTC3236의 α-galactosidase에 의한 Oligo당 생성의 최적조건

        왕지원,김경민,강대중,강국희,정재록 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

        Bifidobacterium angulatum KCTC3236 showed the highest α-galactosidase activity among the tested strains of Bifidobacteria( data not shown). Optimum culture conditions for the maximum production of α-galactosidase were as follows. The culture media was MRS broth containing 1% galactose as a carbon source and its initial pH was 7.0. The incubation temperature and time were 37℃, 21 hours respectively. Melibiose have been used as substrate. The formation of oligosaccharides was maximum at 40℃ when the substrate concentration was 30%. And the unit of α-galactosidase was 40 unit. Finally, we could observe the maximum production of oligosaccharides after 14 hours' enzyme reaction.

      • KCI등재

        수종의 저장용액에서 치주인대세포의 생존율 비교

        최원경,최병재,최형준,이종갑 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2

        치아탈구 발생시 사고 현장에서 쉽게 구할 수 있는 저장용액을 이용하여 치주인대세포의 생존율을 비교해보고자 치주인대세포를 10%FBS함유 α-MEM에서 배양한 후 α-MEM에서 저장한 군을 대조군으로 하고 동일한 조건하에서 저온살균된 우유군, 멸균된 생리적 식염수군, 비자극성 타액군, 건조된 상태로 방치한 군을 실험군으로 하여 25℃ 실온에서 세포 배양 30, 60, 90, 120, 180분 후에 각 군에 대해 MTTassay를 시행하여 다음과 같은 결론을 얻었다. 1. 각 조사시점에서 실험군간의 비교에서는 모두 유의성있는 차이를 보였으며 저온살균된 우유군에서 치주인대세포의 생존율이 가장 높았고, 멸균된 생리적 식염수군, 비자극성 타액군, 건조된 상태로 방치한 군 순으로 점차 감소하였다.(P<0.05) 2. 각 실험군내에서 조사시점간의 비교에서는 치주인대세포의 생존율이 시간이 경과함에 따라 유의성있게 감소하였으나(p<0.05). 저온살균된 우유군에서 90-120분 경과 사이. 멸균된 생리적 식염수군에서 60-90분과 120-180분 경과 사이에서는 유의성있는 차이는 없었다.(P>0.05) 이상의 결론을 종합해볼 때 외상으로 인한 치아탈구 발생시 저장용액으로 HBSS가 추천되고 있으나 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 저온살균된 우유에 탈구된 치아를 담가오도록 추천할 수 있겠다. Preservation of the remaining periodontal ligament cells on an avulsed tooth is very important to the successful outcome of replantation. HBSS is recommended as the most suitable storage medium for the avulsed tooth that cannot be replanted immediately. But their availability near the site of an accident is doubtful. The purpose of this in vitro study was to compare periodontal ligament cells stored in different storage media obtained easily on th spot. Human periodontal ligament cells were collected from the premolar teeth extracted for orthodontic treatment. Cells were cultured in α-MEM culture medium containing 20% FBS, at 37℃ 100% humidity, in a 5% CO₂2 incubator. Cells were cultured in 96 well culture plate, 5×10⁴cells per well with α-MEM and incubated for 24 hours.After discarding the medium, those cells were cultured in α-MEM contained with 10% FBS, pasteurized milk, sterilized saline, unstimulated saliva and bench-dried state at 25℃ room temperature for 30, 60, 90, 120, 180 minutes respectively. And then each group was measured using MTT assay. The results were as follows. 1. Between the group of each time, there was statistically significant difference. Periodontal ligament cells viability was highest in pasteurized milk and was reduced stepwisely in sterilized saline, unstimulated saliva and bench-dried state(p<0.05) 2. between the time of each group, there was statistically significant difference (p<0.05) but was no statistically significant difference at 90 - 120 minutes in pasteurized milk and at 60 - 90 minutes and 120 - 180 minutes in sterilized saline(p>0.05). In conclusion, HBSS as storage medium of an avulsed tooth is not practical on the spot. Insteadily pasteurized milk can be recommended to maintain the periodontal ligament cells viability.

      • 骨髓炎 原因菌의 感受性에 對한 細菌學的 考察

        張元在,尹太鉉 조선대학교 1981 The Medical Journal of Chosun University Vol.6 No.-

        Osteomyelitls is the most common bone infection of which pathogenic organisms reach the bone by direct extension from neighboring infected soft tissues, through penetrating wounds and open fractures, or by the blood stream. Hematogenous osteomyelitis, by far the most common type that caused by staphylococcus aureus, is predominantly a disease of infants and children than of adults. The author analysed pathogenic organisms on the 121 cases of osteomyelitis who had been treated at the Department of Orthopedic Surgery, Chosun University Hospital, during the period of 6 years from Jan. 1974 to Sept. 1980 and the following results were obtained. 1. The sex ratio between the male and female was 2.4:1 and the average age was 26 years old. 2. The most common site of the osteomyelitis was the tibia (40.5%) and the next was the femur(28.1%) and the humerus(14.1%). 3. The most common causative organism was staphylococcus aureus(61.5%), and the next was pseudomonas species(17.0%) 4. The drug of choice for antibiotics on the sensitivity test for the staphylococcus aureus was the cephalosporin and the minocin. But penicillin and tetracycline were resistant. 5. The gentamicin and dibekacin sulfate were very sensitive antibiotics for the pseudomonas species. Other antibiotics were resistant. 6. Gentamicin, minocin and cephalosporin were very sensitive antibiotics for Escherichia coli. But penicillin, tetracycline and liacocin were resistant. 7. Gentamicin, cephalosporin and minocin were very sensitive antibiotics for the mixed infection.

      • 중소도시 토지이용수요추정의 실증적 분석

        김재원 대불대학교 2001 論文集 Vol.7 No.1

        Most of cities in Korea are using the following method of estimating demands for residential, commercial and industrial land within their urbanization ares: They are putting to use a calculation formula for by-use land areas on the basis of per capita areas required and the scale of the population. But the method above fails to take into consideration the features of indexes presenting the characteristics of cities and is being unanimously applied to most of the cities. Accordingly, this study have extracted factors related to areas in use on the basis of various kinds of city composition materials, calculating models designed to estimate demands for land use with those factors as variables. Residential areas turned out to be subjected to the greatest differences in the areas of land in use, while commercial and residential areas turned out to make few differences. And the findings show that the areas of residential regions are most influenced by the exterior factors of cities in urban planning and that it is necessary that the areas of residential areas should first be expanded for the cause of the artificial development of cities. And the calculation formula for by-use land areas turned out be appropriate for the calculation of the areas of industrial regions. The models for calculating residential areas should take into consideration the exterior factors of cities as well as urban-organization materials.

      • 開途國 外債問題: 債務不履行과 債務輕減

        柳在元 建國大學校 經濟經營硏究所 1996 商經硏究 Vol.21 No.1

        A striking feature of international borrowing is the existence of sovereign risk. The deveolping-country debt crisis in the 1980s highlights the risk of default. This paper aims to clarify key issues central to the debt crisis and draw some lessons from it for coping with the debt problem. After reviewing causes and development of the debt crisis, it focuses on interrelated, but seemingly contradictory concepts : default and debt reduction. The paper proposes a two-period interemporal model under uncertainty of second-period income. The utility maximizing debtor compares the cost of default with the benefits from default. Accordingly, the default risk is assumed to depend on factors affecting the debt servicing capacity and the debt burden. A empirical study on the 16 highly indebted countries confirms that the default risk is positively related to the amount of debt and negatively to the economic growth rate. The debt reduction was adopted in the lated 1980s in order to deal with the debt crisis. It is shown that the market-based debt reduction is possible only if the debt reduces the default risk so that the expected amount of debt service increases. The following empirical study reveals that debt reduction during the debt crisis was actually concentrated on a few countries with the relatively sound debt servicing capacity. The debt burden is still too high for many developing countries, and negative external shocks may rekindle the debt crisis. For developing countries, it is prerequisite to improve the debt-servicing capacity in order to induce foreign capital. From the creditor's viewpoint, a more effective enforcement mechanism ensuring repayment of debt is needed in order to resolve the debt problem once and for all.

      • KCI등재후보

        석유화학공업 종사자의 유전독성 위험성 평가

        안기원,박정선,서순팔,문재동,조진형 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

        In order to evaluate the genotoxic hazard among workers potentially exposed to low level petrochemical substances, the analyses of micronuclei(MN) and sister chromatid exchanges(SCEs) in lymphocytes were performed in 46 male workers(as exposed group) and 46 nonexposed subjects(as control group). Mean frequencies of MN and SCEs(respectively, 12.9/1000 cells and 6.5/cell) in exposed group were very significantly higher than those(10.2/1000 cells and 5.4/cell) in control group. And there were also significant differences in mean frequencies of MN and SCEs adjusted for age, employment duration, smoking, and drinking between two groups. Median frequencies of MN and SCEs in exposed group were very significantly higher than those in control group. Frequencies of SCEs were higher in smokers than in non-smoker. Frequencies of MN in smokers, however, were similiar to those of non-smoker. Interaction between exposure and smoking on MN and SCEs induction was not observed. The results suggest that there is genotoxic hazard in high risk group like workers handling carcinogens in petrochemical plants and the analyses of MN and SCEs are useful biomarkers for the exposure to hazard substances even at the level below the exposure limit.

      • 핸드볼 選手의 上肢 伸筋 및 持久力 向上을 위한 等速性 트레이닝의 效果 미치는 效果

        柳在忠,尹誠源 慶熙大學校 社會體育硏究所 1994 體育科學論叢 Vol.- No.7

        The purpose of this study examined the effect on Isokinetic training on the muscle strength and endurance of the upper limb for Hand Ball player. Subjects were 8 handball players(22.7±1.84yrs) of H University team Orthoton(LUmax : U.S.A) were used for 8 weeks of training and Cybex 6000 system made use of in measurement every 2 weeks. The results of analysis on the data obtained from each measurement were as follows: 1. Isoknetic training during 8 weeks is effective in the development of muscular strength and endurance of upper limb. 2. The effective on the muscular strength and endurance of upper limb appered from 2 weeks for the isokinetic training. From the results, it is suggested that the isokinetic training is effective in improving the muscle strength and endurance of the extension of the upper limb. However Muscle function includes muscle strength, muscle power and muscle endurance and its function can work more improve when the careful isokinetic training is programing.

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