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Mitchell Wendt,허강준 한국실험동물학회 2016 Laboratory Animal Research Vol.32 No.4
Our research sought to characterize the phylogeny of Pseudomonas aeruginosa isolated from pet Chinese stripe-necked turtles (Ocadia sinensis) to better understand its evolutionary relation to other isolates and increase understanding of a potential zoonotic pathogen transmitted through direct contact with pet turtles. Thirty-one Pseudomonas aeruginosa isolates were obtained from both immature and adult turtles sold in pet shops in Korea. To characterize the phylogenic position of Chinese stripe-necked turtle-borne P. aeruginosa relative to other strains, multilocus sequence typing (MLST) analysis was performed due to the accessibility and breadth of MLST databases. Seven housekeeping genes (acsA, aroE, guaA, mutL, nuoD, ppsA, and trpE) were sequenced and the results were compared with data from the MLST database. The genes were further used for phylogenetic analysis of P. aeruginosa using concatenated gene fragments. Both rooted and unrooted phylogenetic trees were generated. Eleven distinct sequence types were present within the isolates among which seven were new. Expanding an unrooted phylogenetic tree to include P. aeruginosa MLST sequences isolated from various other geographic locations and sources revealed a divergent cluster containing the majority of isolates obtained from turtles. This suggests that P. aeruginosa strains particularly well-adapted for inhabiting turtles occupy a distinct phylogenetic position.
PRIMENESS AND PRIMITIVITY IN NEAR-RINGS
Gerhard Wendt 대한수학회 2021 대한수학회지 Vol.58 No.2
In near-ring theory several different types of primitivity exist. These all imply several different types of primeness. In case of near-rings with DCCN most of the types of primeness are known to imply primitivity of a certain kind. We are able to show that also so called $1$-prime near-rings imply $1$-primitivity. This enables us to classify maximal ideals in near-rings with chain condition with the concept of $1$-primeness which leads to further results in the structure theory of near-rings.
Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria
박준우,Mitchell Wendt,허강준 한국실험동물학회 2016 Laboratory Animal Research Vol.32 No.2
The antibacterial activities of the essential oil of Eucalyptus globulus (EOEG) was determined against 7 fish pathogenic bacteria (Edwardsiella tarda, Streptococcus iniae, S. parauberis, Lactococcus garviae, Vibrio harveyi, V. ichthyoenteri and Photobacterium damselae) obtained from farmed olive flounder. The inhibitory activity was evaluated by three methods: Disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the disc diffusion test, as the concentration of EOEG (5-40 µg) rises, the inhibitory zone increases in size. Compared with amoxicillin, tetracycline and chloramphenicol, EOEG showed similar antibacterial activity. The MIC of EOEG ranged from 7.8 to 125 mg/mL and MBC values ranged from 62 to 250 mg/mL. These results show that EOEG has antimicrobial activity against all seven bacteria, but there was no marked difference between each genus. From these results, it is suggested that EOEG can be used as an antimicrobial agent against fish bacterial diseases in the fish industry.
Design Studies for a High Resolution Cold Cavity Beam Position Monitor
Seunghwan Shin,Wendt, Manfred IEEE 2010 IEEE transactions on nuclear science Vol.57 No.4
<P>In order to preserve a low emittance beam along the ~11 km long main linacs of the International Linear Collider (ILC), precise monitoring and control of the beam orbit is mandatory. A resolution <; 1 μm is required for the beam position monitors (BPM), which are located inside the cryomodules, i.e. operated at cryogenic temperatures. By using the electromagnetic field simulation programs (CST Studio Suite), we have designed a high resolution cold cavity BPM, operating in the L-Band at a 1.3 GHz dipole-mode frequency. This enables the measurement of the beam position with a Project-X like beam structure, as well as with ILC beam parameters at the superconducting RF test accelerator in the Fermilab New Muon Lab (NML) building. The results of the design studies predicts a resolution potential of a few hundred nanometers with a signal decay time of 50 ns.</P>
Prevalence of Salmonella spp. in pet turtles and their environment
백두산,신기욱,Mitchell Wendt,허강준 한국실험동물학회 2016 Laboratory Animal Research Vol.32 No.3
Pet turtles are known as a source of Salmonella infection to humans when handled in captivity. Thirty four turtles purchased from pet shops and online markets in Korea were examined to determine whether the turtles and their environment were contaminated with Salmonella spp. Salmonella spp. were isolated from fecal samples of 17 turtles. These isolates were identified as S. enterica through 16S rRNA gene sequencing. The isolation rate of Salmonella spp. from the soil and water samples increased over time. We concluded that a high percentage of turtles being sold in pet shops were infected with Salmonella spp., and their environments tend to become contaminated over time unless they are maintained properly. These results indicate that pet turtles could be a potential risk of salmonellosis in Korea.