The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny

Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7

In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Investigation of Cu Interlayer on Joint Formation of Ti/Mg Bimetal Fabricated by Liquid–Solid Compound Casting Process

Fulin Wen,Jianhua Zhao,Kaiqing Feng,Miaowang Yuan,Dengzhi Zheng,Cheng Gu,Bei Xu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.7

In this paper, TC4/AZ91D bimetallic composites has been prepared by liquid–solid compound casting process adaptingvarious thickness of Cu coating. The effects of Cu coating thickness on the evolution of interface microstructures and jointmechanical properties are investigated. The results indicate that metallurgical bonded joints are obtained with Cu coatingthickness ranging from 36.7 to 51.4 μm. With the increase of Cu coating thickness, the interface microstructure evolvesfrom δ-Mg + Mg2Cueutectic structure to Mg–Cu intermetallic compound (IMC) Cu2Mg+ Mg2Cuand Mg–Al–Cu ternaryintermetallic compound. The calculation results of formation enthalpy and chemical potential of Mg–Al–Cu system suggestthat Cu element prefers to react with Mg element and formed Mg–Cu IMC. In particular, when Cu coating thickness reaches36.7 μm, the average shear strength of the bimetal reaches a maximum of 65.3 MPa. Further increasing Cu coating thicknessleads to the generation of thick Mg2CuIMC layer and Mg2Cu+ Cu2Mgmixed IMC layer which are proved to be harmful tothe shear strength of TC4/AZ91D bimetals. All the fracture surfaces of the bimetallic composites exhibite to have a brittlefracture morphology. However, the fracture location is different with each other. For Cu coating thickness of 36.7 μm, theinterface fractures at the δ-Mg + Mg2Cueutectic structure, while the interface fractures at the Cu2Mg+ Mg–Al–Cu ternaryintermetallic layers when Cu coating thickness is 44.2 μm and 51.4 μm.

경영에서 grab start와 track start의 운동학적 분석

김연정,이민형,원문학,김헌수 한국운동역학회 2000 한국운동역학회지 Vol.10 No.1

본 연구는 남녀 수영 선수 6명씩을 대상으로 grab start와 track start를 실시하여 두 집단간과 두 출발법간의 동작 특성을 운동학적으로 분석한 후 변인들을 정량화 하여 선수들에게 출발법에 대한 심층적인 이해를 제공하고 효과적인 훈련에 기여하도록 하는 것이 목적이며 실험으로 카메라 4대를 이용하였으며 분석을 위해 권(1993)이 개발한 Kwon3D 동작 분석 프로그램으로 3차원 영상 분석을 실시하였다. 결과의 통계 처리 자료로는 SPSS 프로그램을 사용하였으며 출발에 관련된 운동학적 변인을 수평방향과 수직방향에서의 인체 중심점 위치와 속도의 변화를 측정하였다. 위치 변화에서는 출발대를 떠나는 지점에서 남자 선수들은 수직 지향을 여자 선수들은 수평 지향을 함을 알 수 있었고 속도 변화에서 출발대를 떠나는 순간 track start가 속도값이 크기는 하나 두 출발법 사이에 유의한 차이는 나타나지 않았다. The research has the aim to analyze and find out kinematic features of grab start and track start between male and femail. With having selected 6 swimmers form each for this experiment four video-cameras(60 frames/sec) and control-box(length-2m, width-1m, hight-1m) to get real space coordinates were used. The DLT method was employed to obtain the 3-dimensional coordinates of the 25 body points for digitizing with Kwon3D Motion Analysis Package developed by Kwon, Young-hoo (1993). SPSS was used for statistic process. Kinematic variable were measured in start: The center of mass of body in horizontal and vertical, variation of velocity, entry distance. The following are the results. 1. In change of the body-center, the starting methods of man and women are same in horizontal directions, but in vertical directions the amount of women´s body changement is decreasing ever, while the amount of man´s body changement is increasing continuously with his foot off the start line. The change of the position and velocity in vertical directions is changing with the same tendency in case of starting ways of both man and women, but in vertical directions man´s amount of changement has steep increasing curve, and women´s one has decreasing curve. 2. Horizontal ANOVA analysis of body-center shows that starting method represent not such difference at taking-off moment but significant difference at initial moving point(F=237.016, p<.01). Vertical analysis shows significant difference in gender and starting method(for each F=31.132, p<.01, f=22.591, p<.01)

Methylenetetrahydrofolate Reductase Genetic Polymorphisms and Esophageal Squamous Cell Carcinoma Susceptibility: A Meta-analysis of Case-control Studies

Wen, Yuan-Yuan,Yang, Shu-Juan,Zhang, Jian-Xing,Chen, Xin-Yue Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Genetic factors and environmental factors play a role in pathogenesis of esophageal squamous cell carcinoma (ESCC). Previous studies regarding the association of folate intake and Methylenetetrahydrofolate reductase C677T polymorphism with ESCC was conflicting. We conducted a meta-analysis to investigate the association of MTHFR C677T and folate intake with esophageal cancer risk. Methods: MEDLINE, EMBASE and the Chinese Biomedical Database were searched in our study. The quality of studies were evaluated by predefined scale, and The association of polymorphisms of MTHFR C677T and folate intake and ESCC risk was estimated by Odds ratio (ORs) with 95% confidence intervals (CIs). Results: 19 studies (4239 cases and 5575 controls) were included for meta-analysis. A significant association was seen between individuals with MTHFR 677 CT [OR(95%)=1.47(1.32-1.63)] and TT [OR(95%)=1.69(1.49-1.91)] genotypes and ESCC risk (p<0.05). Low intake of folate had significantly higher risk of esophageal cancer among individuals with CT/TT genotype [OR(95%)=1.65(1.1-2.49)], while high intake of folate did not find significant high risk of esophageal cancer among individuals with CT/TT genotype [OR(95%)=1.64 (0.82-3.26)]. Conclusions: Our meta-analysis indicated the folate intake and MTHFR 677CT/TT are associated with the risk of ESCC, and folate showed a significant interaction with polymorphism of MTHFR C677T.

Large-scale production of (2,4-DHB)nM micro-nano spheres by spray drying and their application as catalysts for ammonium perchlorate

Wen-yuan Zhao,Tong-lai Zhang,Li-nong Zhang,Li Yang,Zun-ning Zhou 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.38 No.-

A series of 2,4-dihydroxybenzoates {(2,4-DHB)nMs, M = Pb(II), Cu(II), Fe(III), Ni(II) and Co(II), n = 2 or 3}micro-nano spheres with controllable size and morphology were successfully prepared by spray dryingmethod with water or acetone as a solvent. TEM and SEM illustrated that the (2,4-DHB)nMs micro-nanospheres had core–shell or solid structure. The formation of the core–shell structure of the samples wasinterpreted in terms of the mechanism of spray drying. The results of laser particle analyzer revealed thatprecursor concentration (PC) and particle size distribution (PSD) of the samples were well fitted withpower function. The thermal decomposition of ammonium perchlorate (AP) in the presence of the (2,4-DHB)nMs micro-nano spheres, investigated by differential scanning calorimetry (DSC), showed theyeffectively lowered the decomposition temperature of AP and exhibited various catalytic performancefor AP decomposition.

454 Pyrosequencing Analysis of Bacterial Diversity Revealed by a Comparative Study of Soils from Mining Subsidence and Reclamation Areas

( Yuan Yuan Li ),( Long Qian Chen ),( Hong Yu Wen ),( Tian Jian Zhou ),( Ting Zhang ),( Xia Li Gao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Significant alteration in the microbial community can occur across reclamation areas suffering subsidence from mining. A reclamation site undergoing fertilization practices and an adjacent coal-excavated subsidence site (sites A and B, respectively) were examined to characterize the bacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomic groups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, and Firmicutes. However, the bacterial communities` abundance, diversity, and composition differed significantly between the sites. Site A presented higher bacterial diversity and more complex community structures than site B. The majority of sequences related to Proteobacteria, Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria, and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes, Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria, and Phycisphaerae originated from site B. The distribution of some bacterial groups and subgroups in the two sites correlated with soil properties and vegetation due to reclamation practice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and total nitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN were important factors shaping the underlying microbial communities. Furthermore, the specific plant functional group (legumes) was also an important factor influencing soil microbial community composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soil bacterial diversity was validated and an association between land ecological system restoration, mostly mediated by microbial communities, and an improvement in soil properties in coalmining reclamation areas was suggested.

ADPRT Val762Ala and XRCC1 Arg194Trp Polymorphisms and Risk of Gastric Cancer in Sichuan of China

Wen, Yuan-Yuan,Pan, Xiong-Fei,Loh, Marie,Tian, Zhi,Yang, Shu-Juan,Lv, Si-Han,Huang, Wen-Zhi,Huang, He,Xie, Yao,Soong, Richie,Yang, Chun-Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: Gastric cancer remains a major health problem in China. We hypothesized that XRCC1 Arg194Trp and ADPRT Val762Ala may be associated with risk. Methods: We designed a multicenter 1:1 matched case-control study of 307 pairs of gastric cancers and controls between October 2010 and August 2011. XRCC1 Arg194Trp and ADPRT Val762Ala were sequenced, and demographic data as well as lifestyle factors were collected using a self-designed questionnaire. Results: Individuals carrying XRCC1 Trp/Trp or Arg/Trp variant genotype had a significantly increased risk of gastric cancer (OR, 1.718; 95% CI, 1.190-2.479), while the OR for ADPRT Val762Ala variant genotype (Ala/Ala or Val/Ala) was 1.175 (95% CI, 0.796-1.737). No gene-gene or gene-environment interactions were found. In addition, family history of cancer and drinkers proportion were higher among cases than among controls (P<0.05). Conclusions: XRCC1 194 Arg/Trp or Trp/Trp genotype, family history of cancer, and drinking are suspected risk factors of gastric cancer from our study. Our findings may offer insight into further similar large gene-environment and gene-gene studies in this region.

Canonical transient receptor potential channels and their modulators: biology, pharmacology and therapeutic potentials

Yuan-Yuan Gao,Wen Tian,Hui-Nan Zhang,Yang Sun,Jing-Ru Meng,Wei Cao,Xiao-Qiang Li 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4

Canonical transient receptor potential channels(TRPCs) are nonselective, high calcium permeability cationicchannels. The TRPCs family includes TRPC1, TRPC2,TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7. These channelsare widely expressed in the cardiovascular and nervoussystems and exist in many other human tissues and celltypes, playing several crucial roles in the human physiologicaland pathological processes. Hence, the emergenceof TRPCs modulators can help investigate these channels’applications in health and disease. It is worth noting that theTRPCs subfamilies have structural and functional similarities,which presents a signifi cant diffi culty in screening anddiscovering of TRPCs modulators. In the past few years,only a limited number of selective modulators of TRPCswere detected; thus, additional research on more potent andmore selective TRPCs modulators is needed. The presentreview focuses on the striking desired therapeutic eff ectsof TRPCs modulators, which provides intel on the structuralmodifi cation of TRPCs modulators and further pharmacologicalresearch. Importantly, TRPCs modulators cansignifi cantly facilitate future studies of TRPCs and TRPCsrelated diseases.

Spaceflight of Platycodon grandiflorum Seeds Changes Ultrastructure of Pedicel and Style Cells

Wen-Yuan Gao(高文遠),Lei Fan(范磊),Kee-Yoeup Paek(白基燁),Shu-Ping Zhao(趙淑萍) 한국원예학회 2000 Horticulture, Environment, and Biotechnology Vol.41 No.6

Color Harmony on 3D Color Configuration

Wen-Yuan Lee,Sin-Jhe Huang,Yi-Wen Chang 한국색채학회 2011 한국색채학회 학술대회 Vol.2011 No.10

The purpose of this study is to see the "harmonious- disharmonious" feeling of color combination on 3D color configuration. A cuboid with side circle shape was used to display 122 color combinations. Each color combination was evaluated on "harmonious-disharmonious" scale by 32 observers. The results showed that color harmony for the color combinations having an achromatic color differed from those having two chromatic colors. For the color combinations having an achromatic color, the color harmony was evoked by sum of lightness. The most disharmonious feeling was evoked when the sum of lightness equal to 85. When both neighboring colors are chromatic colors, color harmony was determined by hue angle difference. Two colors having similar hue appeared harmonious feeling.