Mitochondrial NDUFA4L2 attenuates the apoptosis of nucleus pulposus cells induced by oxidative stress via the inhibition of mitophagy

Wen-Ning Xu,Huo-Liang Zheng,Run-Ze Yang,Tao Liu,Wei Yu,Xin-Feng Zheng,Bo Li,Sheng-Dan Jiang,Lei-Sheng Jiang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The main pathological mechanism of intervertebral disc degeneration (IVDD) is the programmed apoptosis of nucleus pulposus (NP) cells. Oxidative stress is a significant cause of IVDD. Whether mitophagy is induced by strong oxidative stress in IVDD remains to be determined. This study aimed to investigate the relationship between oxidative stress and mitophagy and to better understand the mechanism of IVDD in vivo and in vitro. To this end, we obtained primary NP cells from the human NP and subsequently exposed them to TBHP. We observed that oxidative stress induced mitophagy to cause apoptosis in NP cells, and we suppressed mitophagy and found that NP cells were protected against apoptosis. Interestingly, TBHP resulted in mitophagy through the inhibition of the HIF-1α/NDUFA4L2 pathway. Therefore, the upregulation of mitochondrial NDUFA4L2 restricted mitophagy induced by oxidative stress. Furthermore, the expression levels of HIF-1α and NDUFA4L2 were decreased in human IVDD. In conclusion, these results demonstrated that the upregulation of NDUFA4L2 ameliorated the apoptosis of NP cells by repressing excessive mitophagy, which ultimately alleviated IVDD. These findings show for the first time that NDUFA4L2 and mitophagy may be potential therapeutic targets for IVDD.

Behavioral Responses of Pregnant Women to the Early Stage of COVID-19 Pandemic in the Network Era in China: Online Questionnaire Study

Wen-sheng Hu,Sha Lu,Meng-yan Xu,Min-cong Zhou,Zhen-ming Yuan,Yue-yue Deng 한국간호과학회 2021 Asian Nursing Research Vol.15 No.3

Purpose: The aim of this study was to examine the behavioral responses of pregnant women during the early stage of Coronavirus Disease 2019 (COVID-19) outbreak. Methods: We recruited 1,099 women to complete an online questionnaire survey from February 10 to February 25, 2020. The subjects were divided into two groups (the pregnant women group and the control group). Results: Concerns about infection: most of the participants watched the COVID-19 news at least once a day. Protective behaviors: the utilization rate of pregnant women (often using various measures) was higher than that of nonpregnant women. Exercise: 30.6% of the pregnant women continued to exercise at home, whereas in the control group, this percentage was 8.4%. Spouse relationship: 38.8% of the subjects’ relationship improved, whereas only 2.3% thought the relationship was getting worse. Conclusion: Pregnant women had some unique behavioral responses different from that of nonpregnant women. It is important to understand the behavioral responses of pregnant women in this network era.

Prediction of Mortality after Emergent Transjugular Intrahepatic Portosystemic Shunt Placement: Use of APACHE II, Child-Pugh and MELD Scores in Asian Patients with Refractory Variceal Hemorrhage

Wen-Sheng Tzeng,Reng-Hong Wu,Ching-Yih Lin,Jyh-Jou Chen,Ming-Juen Sheu,Lok-Beng Koay,Chuan Lee 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.5

Objective: This study was designed to determine if existing methods of grading liver function that have been developed in non-Asian patients with cirrhosis can be used to predict mortality in Asian patients treated for refractory variceal hemorrhage by the use of the transjugular intrahepatic portosystemic shunt (TIPS) procedure. Materials and Methods: Data for 107 consecutive patients who underwent an emergency TIPS procedure were retrospectively analyzed. Acute physiology and chronic health evaluation (APACHE II), Child-Pugh and model for end-stage liver disease (MELD) scores were calculated. Survival analyses were performed to evaluate the ability of the various models to predict 30-day, 60-day and 360-day mortality. The ability of stratified APACHE II, Child-Pugh, and MELD scores to predict survival was assessed by the use of Kaplan-Meier analysis with the log-rank test. Results: No patient died during the TIPS procedure, but 82 patients died during the follow-up period. Thirty patients died within 30 days after the TIPS procedure; 37 patients died within 60 days and 53 patients died within 360 days. Univariate analysis indicated that hepatorenal syndrome, use of inotropic agents and mechanical ventilation were associated with elevated 30-day mortality (p < 0.05). Multivariate analysis showed that a Child-Pugh score > 11 or an MELD score > 20 predicted increased risk of death at 30, 60 and 360 days (p < 0.05). APACHE II scores could only predict mortality at 360 days (p < 0.05). Conclusion: A Child-Pugh score > 11 or an MELD score > 20 are predictive of mortality in Asian patients with refractory variceal hemorrhage treated with the TIPS procedure. An APACHE II score is not predictive of early mortality in this patient population. Objective: This study was designed to determine if existing methods of grading liver function that have been developed in non-Asian patients with cirrhosis can be used to predict mortality in Asian patients treated for refractory variceal hemorrhage by the use of the transjugular intrahepatic portosystemic shunt (TIPS) procedure. Materials and Methods: Data for 107 consecutive patients who underwent an emergency TIPS procedure were retrospectively analyzed. Acute physiology and chronic health evaluation (APACHE II), Child-Pugh and model for end-stage liver disease (MELD) scores were calculated. Survival analyses were performed to evaluate the ability of the various models to predict 30-day, 60-day and 360-day mortality. The ability of stratified APACHE II, Child-Pugh, and MELD scores to predict survival was assessed by the use of Kaplan-Meier analysis with the log-rank test. Results: No patient died during the TIPS procedure, but 82 patients died during the follow-up period. Thirty patients died within 30 days after the TIPS procedure; 37 patients died within 60 days and 53 patients died within 360 days. Univariate analysis indicated that hepatorenal syndrome, use of inotropic agents and mechanical ventilation were associated with elevated 30-day mortality (p < 0.05). Multivariate analysis showed that a Child-Pugh score > 11 or an MELD score > 20 predicted increased risk of death at 30, 60 and 360 days (p < 0.05). APACHE II scores could only predict mortality at 360 days (p < 0.05). Conclusion: A Child-Pugh score > 11 or an MELD score > 20 are predictive of mortality in Asian patients with refractory variceal hemorrhage treated with the TIPS procedure. An APACHE II score is not predictive of early mortality in this patient population.

On the signed total domination number of generalized Petersen graphs P(n,2)

Wen-Sheng Li,손무영,Hua-Ming Xing 대한수학회 2013 대한수학회보 Vol.50 No.6

Let G = (V,E) be a graph. A function f : V → {−1,+1} defined on the vertices of G is a signed total dominating function if the sum of its function values over any open neighborhood is at least one. The signed total domination number of G, γs t (G), is the minimum weight of a signed total dominating function of G. In this paper, we study the signed total domination number of generalized Petersen graphs P(n, 2) and prove that for any integer n ≥ 6, γs t (P(n, 2)) = [수식], where t ≡ n(mod 3) and 0 ≤ t ≤ 2.

Development of Sensing Signal Transmission and System in Intelligent Field

( Wen-luh Huang ),( Shu-yu Yang ),( Wen Sheng Chen ),( Yan-ru Huang ),( Yi-chen Wu ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

There is a problem which is large manpower consumption on contract sweet potato field when measure the soil moisture, control weeds, pests and diseases currently. In addition, serious problems of aging and labor shortage are especially troublesome in domestic rural areas. Therefore, we develop a management system combined sensor, single-chip control module and real-time irrigation module to meet the needs. Throughout the wireless signal transmission, the management of operating system with instant control and output data achieves intelligent control. At present, the system has been able to collect moisture data from field and to do the corresponding treatment immediately. At the same time, upload the data to cloud, then we can monitor field in real-time. With the establishment of the database, we have been to achieve long-term or short-term analysis and management. We really hope that the result can enhance the intelligence of agricultural technology, increase farmers profits, sustain development of agriculture and keep competitiveness.

Endovascular Treatment for Iliac Vein Compression Syndrome: a Comparison between the Presence and Absence of Secondary Thrombosis

Wen-Sheng Lou,Jian-Ping Gu,Xu He,Liang Chen,Hao-Bo Su,Guo-Ping Chen,Jing-Hua Song,Tao Wang 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.2

Objective: To evaluate the value of early identification and endovascular treatment of iliac vein compression syndrome (IVCS), with or without deep vein thrombosis (DVT). Materials and Methods: Three groups of patients, IVCS without DVT (group 1, n = 39), IVCS with fresh thrombosis (group 2, n = 52) and IVCS with non-fresh thrombosis (group 3, n = 34) were detected by Doppler ultrasonography, magnetic resonance venography, computed tomography or venography. The fresh venous thrombosis were treated by aspiration and thrombectomy, whereas the iliac vein compression per se were treated with a self-expandable stent. In cases with fresh thrombus, the inferior vena cava filter was inserted before the thrombosis suction, mechanical thrombus ablation, percutaneous transluminal angioplasty, stenting or transcatheter thrombolysis. Results: Stenting was performed in 111 patients (38 of 39 group 1 patients and 73 of 86 group 2 or 3 patients). The stenting was tried in one of group 1 and in three of group 2 or 3 patients only to fail. The initial patency rates were 95% (group 1), 89% (group 2) and 65% (group 3), respectively and were significantly different (p = 0.001). Further, the six month patency rates were 93% (group 1), 83% (group 2) and 50% (group 3), respectively, and were similarly significantly different (p = 0.001). Both the initial and six month patency rates in the IVCS patients (without thrombosis or with fresh thrombosis), were significantly greater than the patency rates of IVCS patients with non-fresh thrombosis. Conclusion: From the cases examined, the study suggests that endovascular treatment of IVCS, with or without thrombosis, is effective. Objective: To evaluate the value of early identification and endovascular treatment of iliac vein compression syndrome (IVCS), with or without deep vein thrombosis (DVT). Materials and Methods: Three groups of patients, IVCS without DVT (group 1, n = 39), IVCS with fresh thrombosis (group 2, n = 52) and IVCS with non-fresh thrombosis (group 3, n = 34) were detected by Doppler ultrasonography, magnetic resonance venography, computed tomography or venography. The fresh venous thrombosis were treated by aspiration and thrombectomy, whereas the iliac vein compression per se were treated with a self-expandable stent. In cases with fresh thrombus, the inferior vena cava filter was inserted before the thrombosis suction, mechanical thrombus ablation, percutaneous transluminal angioplasty, stenting or transcatheter thrombolysis. Results: Stenting was performed in 111 patients (38 of 39 group 1 patients and 73 of 86 group 2 or 3 patients). The stenting was tried in one of group 1 and in three of group 2 or 3 patients only to fail. The initial patency rates were 95% (group 1), 89% (group 2) and 65% (group 3), respectively and were significantly different (p = 0.001). Further, the six month patency rates were 93% (group 1), 83% (group 2) and 50% (group 3), respectively, and were similarly significantly different (p = 0.001). Both the initial and six month patency rates in the IVCS patients (without thrombosis or with fresh thrombosis), were significantly greater than the patency rates of IVCS patients with non-fresh thrombosis. Conclusion: From the cases examined, the study suggests that endovascular treatment of IVCS, with or without thrombosis, is effective.

多義構造に関する中日対照研究

盛文忠(Sheng, Wen-zhong) 한국일본어학회 2015 日本語學硏究 Vol.0 No.45

다의적 구조와 어순유형론은 밀접한 연관이 있으며 어순유형론은 많은 언어표현들이 다의성을 띠는 원인이다. 보편적 어순을 갖고 있는 언어(대다수 언어와 일치하는 어순을 갖고 있는 언어)와 보편적이지 않은 어순을 갖고 있는 언어(대다수 언어의 어순과 일치하지 않은 언어)를 비교할 때 전자의 다의적 구조의 수량은 비교적 적은 편이나 후자의 다의적 구조는 많은 편이다. 그 외, 전자(예하면 일본어)의 다의적 구조는 기본적으로 생성능력이 없는 반면에 후자(예하면 중국어)의 다의적 구조는 생성능력이 아주 강하다. Ambiguous constructions are closely related with types of word orders. Many ambiguous constructions coming into being are due to the typology of word order. By virtue of the comparison between harmonious word order language (the word order of whichis similar to the ones of most languages in the world) and inharmonious word order language (the word order of which is dissimilar to the ones of most languages in the world), this study attempts to show that the former possesses less amounts of ambiguous constructions, andthe latter has more amounts of ambiguous constructions. In addition, the former’s (such as Japanese) ambiguous constructions hardly have any productivity, while the latter’s (such as Chinese) ambiguous constructions mostly have very high productivity.

Pyogenic Liver Abscess as a Warning Sign for Primary Liver Cancer: A Nationwide Population-based Study

Huang, Wen-Kuan,Lin, Yung-Chang,Chiou, Meng-Jiun,Yang, Tsai-Sheng,Chang, John Wen-Cheng,Yu, Kuang-Hui,Kuo, Chang-Fu,See, Lai-Chu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Background: There have been no large-scale population-based studies to estimate the subsequent risk of primary liver cancer (PLC) among patients with pyogenic liver abscess (PLA). This study aimed to provide relevant data. Materials and Methods: The Taiwan Longitudinal Health Insurance Database for the years 2000 and 2005 was used. The PLA group were adult inpatients who were newly diagnosed with PLA from 2000 to 2008. The control group was randomly selected and matched with the PLA group in terms of age, sex, and date in which medical treatment was sought other than for PLA. Results: There were 1,987 patients each in the PLA and control groups. In total, 56 had PLC, 48 (2.4%, 601.5 per 100,000 person-years) from the PLA group, and 8 from the control group. After adjusting for potential covariates, the hazard ratio of PLC for the PLA group was 3.4 times that of the control group (95% confidence interval = 1.6-7.3, p <0.001). The PLC risk for the PLA group was significantly higher within the first year after PLA diagnosis (hazard ratio: 35.4) as compared with the control group and became insignificant (hazard ratio: 2.0, 95% confidence interval = 0.8-4.9) more than one year after PLA diagnosis. Conclusions: Patients with PLA have a higher rate of PLC than matched controls, especially within the first year after the diagnosis of PLA, suggesting PLA is a warning sign for PLC.

ON THE SIGNED TOTAL DOMINATION NUMBER OF GENERALIZED PETERSEN GRAPHS P(n, 2)

Li, Wen-Sheng,Xing, Hua-Ming,Sohn, Moo Young Korean Mathematical Society 2013 대한수학회보 Vol.50 No.6

Let G = (V,E) be a graph. A function $f:V{\rightarrow}\{-1,+1\}$ defined on the vertices of G is a signed total dominating function if the sum of its function values over any open neighborhood is at least one. The signed total domination number of G, ${\gamma}^s_t(G)$, is the minimum weight of a signed total dominating function of G. In this paper, we study the signed total domination number of generalized Petersen graphs P(n, 2) and prove that for any integer $n{\geq}6$, ${\gamma}^s_t(P(n,2))=2[\frac{n}{3}]+2t$, where $t{\equiv}n(mod\;3)$ and $0 {\leq}t{\leq}2$.

The Research and Application of Modular Mobile Phone Web Front-end Based on MVCS Mode

Zhang Wen-sheng,Chen Hong 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.5

With the popularity of mobile devices in recent years, the application of mobile phone Web front-end has become a research hotspot. This paper has constructed the MVCS (Model-View-Controller-Store) model by taking the MVC model as the framework, the ExtJS modular as the basis, and data storage (Store) as the data container, explained by taking the book information system the methods and steps of adopting such model to complete the design of modular mobile phone Wed front-end, and displayed the real-time data chart of mobile phone web front-end. Finally, it also has discussed the research trend.