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Wei Lian,Min Gan 한국생물공학회 2024 Biotechnology and Bioprocess Engineering Vol.29 No.1
The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using Coff ea arabica leaf extract. The AgNPs prepared by green route from C. arabica leaf were characterized through UV–visible, X-ray diff raction, transmission electron microscopy and energy-dispersive electron spectroscopy. Later, the prepared NPs were conjugated with cysteamine– folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the eff ective inhibition of folic acid receptor using free folic acid substrate. The outcome of the present study suggests that the fabricated cysteamine–folic acid-conjugated silver nanoparticles could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.
Lian-feng Wei,Chen Bao,Shi-zhong Wang,Yong Zheng,Meng-bin Zhou 한국원자력학회 2020 Nuclear Engineering and Technology Vol.52 No.8
Low cycle fatigue tests on the hydrogenated welding seam of ZreSneNb alloy at room temperature and 360 C had been carried out by using the funnel-shaped flat specimens. The relationships between nominal stress & strain directly measured across the funnel and local stress & strain at the root of the funnel are given by considering cyclic plasticity correction. The results show that the fatigue resistance of welding seam at room temperature is only slightly better than that at 360 C. Probabilistic fatigue life curves are obtained by using a two-parameter power function.
Wei Lian,Yan Liu,Wenjie Wang,Yangtao Dong,Sheng Wang,Zhenying Liu,Yin Liu 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.2
Environmentally friendly low-cost porous ceramics are prepared with 98.2 wt% mining waste as the raw materials at 1180 °C. The glass powder forms liquid phase at high temperatures, promoting the densification of the materials. X-ray diffraction (XRD) results show that the formation of mullite is affected by sintering temperature and content of liquid phase. The enhancement in densification is verified by the scanning electron microscopy (SEM) analysis results. According to the evolution of pore structure, the effect of the glass powder on the structure is confirmed. The number ratio of pores of < 8 μm to those of 8 ~ 20 μm is varied from 7:2 to 2:7. The minimum apparent porosity is 31.22 %, while the maximum density and linear shrinkage are 1.75 g/cm 3 and 8.48 %, respectively. The flexural strength (three-point bending method) was increased from 7.47 MPa to 28.36 MPa, indicating that the addition of glass powder could increase mechanical properties of the porous ceramics. Therefore, solid wastes can be used as the resource to develop porous mullite ceramics.
Lian, Hai-Wei,Zhou, Yun,Jian, Zhi-Hong,Liu, Ren-Zhong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23
Background: MicroRNAs, small noncoding RNA molecules, can regulate mammalian cell growth, apoptosis and differentiation by controlling the expression of target genes. The aim of this study was to investigate the function of miR-323-5p in the glioma cell line, U251. Materials and Methods: After over-expression of miR-323-5p using miR-323-5p mimics, cell growth, apoptosis and migration were tested by MTT, flow cytometry and cell wound healing assay, respectively. We also assessed the influence of miR-323-5p on the mRNA expression of IGF-1R by quantitative real-time reverse transcriptase PCR (qRT-PCR), and on the protein levels by Western blot analysi. In addition, dual-luciferase reporter assays were performed to determine the target site of miR-323-5p to IGF-1R 3'UTR. Results: Our findings showed that over-expression of miR-323-5p could promote apoptosis of U251 and inhibit the proliferation and migration of the glioma cells. Conclusions: This study demonstrated that increased expression of miR-323-5p might be related to glioma progression, which indicates a potential role of miR-323-5p for clinical therapy.
Wei Zhou,Zhi-yuan Wei,Guang-fei Wang,Kang-ning Han,Ran Liu,Lian-Hua Ma 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4
Accurate characterisation of transverse tensile deformation and damage evolution is of importance for evaluatingthe failure behaviors of three-dimensional (3D) braided composites. In the present study, a finite element method (FEM) andseveral non-destructive testing methods including acoustic emission, digital image correlation, and infrared thermography aredeveloped to investigate the transverse tensile deformation and damage evolution of 3D five-directional braided composites. In the finite element approach, a matrix-impregnated fiber bundles (MIFB) model and a representative volume cell (RVC)model, which take into account the fiber bundles and matrix, are respectively established to predict the effective mechanicalproperties of fiber bundles and simulate the deformation and progressive damage of such composites. The damaged locationsand the failure modes including matrix crack, fiber debonding and shear fracture of fiber are predicted and verified byexperimental tests. The non-destructive tests show that the transverse tensile fracture process can be divided into four stageswhich correspond to acoustic emission signals severally. The combination of the FEM based numerical modeling andmultiple non-destructive characterisation methods can accurately monitor the deformation and damage behaviors of 3Dbraided composites under transverse tensile loads and thus provide a reference for structural health monitoring of compositesin practical application.
( Wei Lian Hu ),( Dehui Dai ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2
Sand tubules, made up of sand grains cemented by microbe-induced calcium carbonate precipitation, have been found in China``s Ningxia Province. Sand tubules grow like a tree``s roots about 40-60 cm below the surface. The properties of sand tubules and their bacterial community were examined. X-Ray diffraction analysis revealed that the sand tubules were associated with crystalline calcite. Scanning electron microscopy showed that the crystalline solid had a lamellar structure and lacked the presence of cells, suggesting that no bacteria acted as nucleation sites, nor that the crystalline solid was formed by the aggregation of bacteria. Denaturing gradient gel electrophoresis analysis showed 11 of the 12 detectable bands were uncultured bacteria by BLAST analysis in the GenBank database, and the rest were closely related to Paenibacillus sp. (100% identity). By cultivation techniques, the only strain isolated from the sand tubule was suggested to be related to Paenibacillus sp.; no archaea were found. Furthermore, Paenibacillus sp. was demonstrated to induce calcium carbonate precipitation in vitro.
염위화(Lian, Wei-Hua),남경숙(Nam, Kyoung-Sook) 한국실내디자인학회 2019 한국실내디자인학회 학술대회논문집 Vol.21 No.1
The downward trend in household size is a global trend. The increase in the number of single applicants is due to a decrease in the marriage rate, an increase in the number of single single households due to the delay of the first marriage, an increase in the number of single households due to divorce or separation, and an increase in the number of elderly households due to aging. It is worth noting that the increase in the number of one-person households is proceeding at a rapid pace, as well as in a variety of marital status and sex and age. Since the beginning of a lifetime in the 20s and 30s by employment or by marriage, the number of people who maintain one"s life till 40-60 is increasing. Based on the background of this study, it is aimed to derive a residential model for middle - aged (39-60) elderly people who are relatively stable. The purpose of this project is to improve the stability of housing and quality of life of the middle-aged 1 person through improvement of the basic performance of housing by developing additional facilities and a resting house space model.
노인요양시설의 색채 시지각디자인이 노인에게 미치는 영향 연구
염위화(Lian, Wei-Hua),남경숙(Nam, Kyung-Sook) 한국실내디자인학회 2019 한국실내디자인학회 학술대회논문집 Vol.21 No.3
Korea entered an aging society in the 21st century. Along with the increase in the elderly population, a variety of nursing homes for the elderly atr on the rise. These elderly care facilities ate designed to accommodate the elderly with various facilities are designed to accommodate the elderly with carious facilities and programs. However, there have not been many studies yet on how these designs affect the elderly onlookers. Therefore, the author wants to study the effects of the design of the aged care facility on the perception of the elderly and be used as a base material for better elderly care facilities in the future. In order to study the effects of visual perception design on the elderly, we want to slaughter the results through brain wave measurememt technology. Analyzing the effects of design on the true elderly, not the visual element, and also on the mind and body, the design will be the basis for the proper introduction of visual design into the aged facility in the future. For the growing elderly population, this is the basic data for a better environment and better life in the future.