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( Wei Bing Zhang ),( Xiao Ling He ),( Hong Na Liu ),( Hui Yuan Guo ),( Fa Zheng Ren ),( Wei Dong Gao ),( Peng Cheng Wen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett?Burman experimental design. Subsequently, an optimal medium was obtained using the Box?Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4?7H2O, 0.1 g/l of MgSO4?7H2O, 0.1 g/l of MnSO4?2H2O, 0.1 g/l of ZnSO4?7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett?Burman design and Box?Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.
CCDC26 Gene Polymorphism and Glioblastoma Risk in the Han Chinese Population
Wei, Xiao-Bing,Jin, Tian-Bo,Li, Gang,Geng, Ting-Ting,Zhang, Jia-Yi,Chen, Cui-Ping,Gao, Guo-Dong,Chen, Chao,Gong, Yong-Kuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
Background: Glioblastoma (GBM) is an immunosuppressive tumor whose median survival time is only 12-15 months, and patients with GBM have a uniformly poor prognosis. It is known that heredity contributes to formation of glioma, but there are few genetic studies concerning GBM. Materials and Methods: We genotyped six tagging SNPs (tSNP) in Han Chinese GBM and control patients. We used Microsoft Excel and SPSS 16.0 statistical package for statistical analysis and SNP Stats to test for associations between certain tSNPs and risk of GBM in five different models. ORs and 95%CIs were calculated for unconditional logistic-regression analysis with adjustment for age and gender. The SHEsis software platform was applied for analysis of linkage disequilibrium, haplotype construction, and genetic associations at polymorphism loci. Results: We found rs891835 in CCDC26 to be associated with GBM susceptibility at a level of p=0.009. The following genotypes of rs891835 were found to be associated with GBM risk in four different models of gene action: i) genotype GT (OR=2.26; 95%CI, 1.29-3.97; p=0.019) or GG (OR=1.33; 95%CI, 0.23-7.81; p=0.019) in the codominant model; ii) genotypes GT and GG (OR=2.18; 95%CI, 1.26-3.78; p=0.0061) in the dominant model; iii) GT (OR=2.24; 95%CI, 1.28-3.92; p=0.0053) in the overdominant model; iv) the allele G of rs891835 (OR=1.85; 95%CI, 1.14-3.00; p=0.015) in the additive model. In addition, "CG" and "CGGAG" were found by haplotype analysis to be associated with increased GBM risk. In contrast, genotype GG of CCDC26 rs6470745 was associated with decreased GBM risk (OR=0.34; 95%CI, 0.12-1.01; p=0.029) in the recessive model. Conclusions: Our results, combined with those from previous studies, suggest a potential genetic contribution of CCDC26 to GBM progression among Han Chinese.
Wei Gu,Yong Liu,Li-Rong Wei,Bing-Kun Dong 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.3
This paper presents a hybrid algorithm for traveling salesman problem. The algorithm is a combination of the genetic algorithm and simulated annealing algorithm; in other words, it is a hybrid algorithm. The combination overcomes the deficiencies of the two algorithms when acting separately. The real distance between customers has been used on the basis of geographical information system (GIS) in order to make the result more suitable in real-life. The algorithm has tested on the examples of international standards. We made a comparison with the result of second nearest neighbor algorithm and genetic optimization algorithm. The test showed that the algorithm proposed in this paper has improved the results.
( Wei-bing Liu ),( Zhi-wei Lin ),( Ying Zhou ),( Bang-ce Ye ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.11
Exopolysaccharides (EPSs) such as capsular polysaccharide (CPS) are important bioactive carbohydrate compounds and are often used as bioenrichment agents and bioabsorbers to remove environmental pollutants like di-n-butyl phthalate (DBP). Among the EPS-producing bacteria, lactic acid bacteria (LAB) have gained the most attention. As generally recognized as safe (GRAS) microorganisms, LAB can produce EPSs having many different structures and no health risks. However, EPS production by LAB does not meet the needs of large-scale application on an industrial scale. Here, the capA gene (encoding CPS biosynthesis protein) was overexpressed in Lactobacillus plantarum P1 to improve the production of EPSs and further enhance the DBP adsorption capability. Compared with P1, the CPS production in capA overexpressed strain was increased by 11.3 mg/l, and the EPS thickness was increased from 0.0786 ± 0.0224 μm in P1 to 0.1160 ± 0.0480 μm in P1-capA. These increases caused the DBP adsorption ratio of P1-capA to be doubled. Overall, the findings in this study provide a safe method for the adsorption and removal of DBP.
Intelligent energy-saving operation of wheel loader based on identifiable materials
Bing-wei Cao,Xinhui Liu,Wei Chen,Kuo Yang,Dan Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.3
This study applies image recognition technology to the shovel loading operation of a wheel loader for the first time. In the proposed method, the wheel loader can automatically select the corresponding engine power curve according to different identifiable materials, thus achieving intelligent energy saving. This study first analyzed the working resistance of the wheel loader, and the analysis results provided theoretical support to the energy-saving operation of the wheel loader. The shovel loading test was carried out for different materials. The test results validated the feasibility of intelligent energy-saving operation of the wheel loader based on identifiable materials. A neural network algorithm based on material identification model was then built and verified, whose material identification rate was 92 %. The model was used as the carrier, and the wheel loader-operating characteristics were considered in view of the existing engine energy-saving mode. Finally, the boom cylinder pressure and bucket angle were used as feedback to future discussion about the intelligent energy-saving operation of the wheel loader. The basic conditions for subsequent designs of wheel loaders are provided.
Bing Wei,Laiming Lu,Hao Li,Yan Xue 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-
A “star-like” architectural wax-crystal modifier based on b-cyclodextrin (P-CD) was successfullydeveloped through a catalytic esterification. This paper mainly investigated the behavior of thiscompound in a waxy oil. The results indicated that P-CD can significantly reduce the oil yield stress anddecrease the wax appearance temperature (WAT). The wax inhibition ratio of P-CD reached up to 50.9%. Cross-Polarized Microscopy (CPM) observations demonstrated that P-CD was capable of hindering waxgrowth. The density function theory (DFT) study verified thefindings from molecular scale, i.e., paraffinswere prone to incorporate with P-CD due to the formation of an extra hydrogen bonding.
Mechanism analysis of pressure fluctuation in wheel loader articulated steering hydraulic system
Bing-wei Cao,Xinhui Liu,Wei Chen,Chen Zhou,Fei-xiang Xu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.10
This paper studies the influence of hinge point positioning of a steering cylinder on the pressure fluctuation of the steering system of a wheel loader. Affected by the hinge point position of the steering cylinder, the main causes of the pressure fluctuation are found to be the stroke difference and the force arm difference. Based on theoretical analysis, the hinge point positioning of the steering cylinder was optimized by using genetic algorithm (GA). The AMEsim simulation model of the steering system was built to verify the accuracy of the GA. The wheel loader was reformed according to the optimized articulated points position. Steering tests showed a significant drop in system pressure fluctuation, the energy consumed is also reduced. Further, the analysis shows that the force arm difference is the main factor causing the pressure fluctuation, providing the theoretical and experimental basis for the design of a steering system of wheel loaders.
Wei Han,Cong Zhang,Xiao-jiao Gao,Hua-bing Wang,Fang Chen,Fang Cao,Yong-wei Hu,Jun Ma,Xing Gu,Hou-zhong Ding 한국유방암학회 2018 Journal of breast cancer Vol.21 No.1
Purpose: Five members of the zinc finger of the cerebellum (ZIC) family—ZIC1, ZIC2, ZIC3, ZIC4, and ZIC5—have been shown to be involved in various carcinomas. Here, we aimed to explore the clinicopathologic and prognostic roles of ZIC family members in invasive breast cancer patients using immunohistochemical analysis, western blotting analysis, and real-time quantitative polymerase chain reaction (RT-qPCR). Methods: A total of 241 female invasive breast cancer patients who underwent radical mastectomy between 2009 and 2011 were enrolled. ZIC proteins in 241 pairs of breast tumors and corresponding normal tissues were investigated using immunohistochemistry and the clinicopathologic roles of proteins were analyzed using Pearson’s chi-square test. Kaplan-Meier curves and Cox regression analysis were also used to analyze the prognostic value of the ZIC proteins. In addition, 12 pairs of fresh-frozen breast tumors and matched normal tissues were used in the western blotting analysis and RT-qPCR. Results: Only ZIC1 expression in normal tissues was obviously higher than that in tumors (p<0.001). On multivariate analysis, ZIC1 expression (in overall survival analysis: hazard ratio [HR], 0.405, 95% confidence interval [CI], 0.233–0.702, p=0.001; in disease-free survival analysis: HR, 0.395, 95% CI, 0.234–0.669, p=0.001) was identified as a prognostic indicator of invasive breast cancer. Conclusion: ZIC1, but not the other proteins, was obviously decreased in breast tumors and associated with clinicopathologic factors. Thus, ZIC1 might be a novel indicator to predict the overall and disease-free survival of invasive breast cancer patients.
Rapid reactive synthesis of Ti₂AlC-TiB₂ composites by spark plasma sintering
Wei-Bing Zhou,Bing-Chu. Mei,Jiao-Qun. Zhu 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.1
In this paper, dense Ti₂AlC/TiB₂ composites were successfully fabricated by a rapid reactive sintering process by a spark plasma sintering (SPS) technique using Ti, Al, TiC and B₄C powders. The microstructure, flexural strength and fracture toughness of the composites were investigated. The experimental results indicate that the Vickers hardness increased with an increase in TiB₂ content. The maximum flexural strength (573 MPa) and fracture toughness (6.2 MPa·m½) were achieved through the addition of 10 vol%TiB₂. The incorporation of a TiB₂ phase makes a positive contribution to its electrical conductivity. In this paper, dense Ti₂AlC/TiB₂ composites were successfully fabricated by a rapid reactive sintering process by a spark plasma sintering (SPS) technique using Ti, Al, TiC and B₄C powders. The microstructure, flexural strength and fracture toughness of the composites were investigated. The experimental results indicate that the Vickers hardness increased with an increase in TiB₂ content. The maximum flexural strength (573 MPa) and fracture toughness (6.2 MPa·m½) were achieved through the addition of 10 vol%TiB₂. The incorporation of a TiB₂ phase makes a positive contribution to its electrical conductivity.