Design and Evaluation of a Social Networking Site (SNS) Supported Collaborative Learning Environment for Vocabulary Learning

YANG Wanping,PAN Yingying 한국교육공학회 2022 Educational Technology International Vol.23 No.1

Vocabulary learning plays an important role in language learning. This study explored a new paradigm based on social networking site (SNS) supported collaborative learning for vocabulary learning. SNS supported collaborative learning (SSCL) can effectively promote learners’ engagement, interest and motivation by providing a more communicative and interactive environment. However, vocabulary learning studies on SSCL mainly focused on the effectiveness and influencing factors, lacking specific instructional strategies. Therefore, this study aims to develop instructional strategies that guide instructors to create an SSCL environment for facilitating vocabulary learning. The final instructional strategies are composed of three stages according to the course process, consisting of 8 general strategies and 21 specific guidelines. The content validity was ensured by four experts in the field of educational technology. The instructional strategies were then applied in an actual classroom with 16 students. The positive responses from the instructor and learners indicated that SSCL can be reasonably incorporated into the current curriculum to provide effective learning opportunities and to promote learners’ vocabulary learning.

Large pore phenylene-bridged mesoporous organosilica with bicontinuous cubic <i>Ia</i>3&cmb.macr;<i>d</i> (KIT-6) mesostructure

Guo, Wanping,Kleitz, Freddy,Cho, Kanghee,Ryoo, Ryong Royal Society of Chemistry 2010 Journal of materials chemistry Vol.20 No.38

<P>Assembly of mesostructured silica using Pluronic P123 triblock copolymer (EO<SUB>20</SUB>-PO<SUB>70</SUB>-EO<SUB>20</SUB>) and n-butanol is a facile synthesis route to the MCM-48-like ordered large mesoporous silicas with the cubic <I>Ia</I>3&cmb.macr;<I>d</I> mesostructure, which are designated KIT-6. This synthesis route has been successfully extended to phenylene-bridged organosilicas from the limit so far for silica, using 1,4-bis(triethoxysilyl)benzene as an organosilica source and re-optimizing the synthesis conditions. In particular, optimal acid concentration and reagent ratios were determined to allow facile synthesis of the bicontinuous cubic mesophase in high yield and high phase purity. The products were characterized by powder X-ray diffraction (XRD), transmission electron microscopy, solid state NMR spectroscopy and analysis of nitrogen sorption at −196 °C using modern non-local density functional theory methods. This synthesis procedure enabled easy fine tuning of pore volume, specific surface area and mesopore size by variation of the hydrothermal aging temperature between 80 and 130 °C. Furthermore, wide angle XRD data suggest short range molecular-scale periodicity in the framework walls originating from regular arrangement of the bridging aromatic groups. These KIT-6 organosilica materials with fully interconnected nanoporous structure would be readily available for applications in heterogeneous catalysis, selective adsorption, and nanostructure design <I>via</I> solid templating approaches.</P> <P>Graphic Abstract</P><P>Phenylene-bridged mesoporous organosilica with a highly ordered <I>Ia</I>3&cmb.macr;<I>d</I> mesostructure was synthesized under acidic conditions by using triblock copolymer and butanol as mesopore-directing surfactant. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0jm01518k'> </P>

A Monascus pilosus MS-1 strain with high-yield monacolin K but no citrinin

Yanli Feng,Wanping Chen,Fusheng Chen 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

Monacolin K (MK) produced by Monascus spp. is mostly used to regulate cholesterol level,while it can be contaminated by citrinin, a mycotoxin yielded by some Monascus strains. To developproduction of citrinin-free MK, an M. sp. MS-1 strain, identified as M. pilosus by morphologies andinternal transcribed spacer sequences analysis, was isolated from red fermented rice. After 14 days offermentation, yields of citrinin-free MK could be up to 0.58 mg/mL and 16.45 mg/g in liquid- and solidstatefermentation products under optimal conditions, respectively. Further determination revealedthat no citrinin biosynthesis related genes such as ctnA, pksCT, ctnE, and ctnR were detected. Thus,HPLC combined with citrinin-related gene analyses can be used for rapid screening of non-citrininproduction Monascus strains.

Identification and expression analysis of ERECTA family genes in grape (Vitis vinifera L.)

Min Liu,Wanping Li,Zhuo Min,Xianghan Cheng,Yulin Fang 한국유전학회 2019 Genes & Genomics Vol.41 No.6

Background ERECTA family (ERf) genes are found in many dicots and monocots, and play important roles in plant developmental processes and stress responses. However, there is little known on ERf genes in grape (Vitis vinifera L.). Objectives The primary objective of this study was to identify the ERf genes in grape, and to analyze their expression profiles in different organs, during development, and in response to hormone treatments and abiotic/biotic stresses. Methods ERf protein sequences of dicots were aligned in the grape genome (V. vinifera cv. Pinot Noir, PN40024, 12X) with Blast server. The locus tags obtained were inputted in NCBI to retrieve corresponding nucleotide and protein accession numbers. The subcellular localization experiment was performed by the transient expression of VvERECTA-GFP and VvERL2-GFP in mesophyll protoplasts of Arabidopsis. The expression levels of ERf genes in grape leaves were detected by qRT-PCR after hormone treatments and abiotic/biotic stresses. Results We first identified the ERf genes in the grape genome, including VvERECTA and VvERL2. Their cDNA full-length sequences were obtained with the accession numbers MG601756 and MG601757. The result of subcellular localization indicated that the fusion proteins of VvERECTA and VvERL2 were localized in the plasma membrane. There were four conserved domains identified in VvERECTA and VvERL2, including a LRRNT-2, a LRR, a transmembrane and a protein kinase domain. The grape ERf genes expressed highly in young aboveground organs. As grape leaves or berries becoming mature, VvERECTA expressed in a declining trend. The transcript abundance of VvERL2 decreased during leaves development, but showed no significant differences during berries development. The hormone treatments of ABA, SA, MeJA and BR could induce the expression of VvERECTA and VvERL2. The treatments of heat, drought, downy and powdery mildew significantly increased the expression levels of the grape ERf genes. Conclusion The grape ERECTA gene family might play crucial roles in response to abiotic and biotic stresses. We provide the first description of the grape ERf genes and the most comprehensive analysis of their expressions in different biological processes.

Facile preparation of a multifunctional fluorescent nanosensor for chemical and biological applications

Wang, Jing,Guo, Wanping,Bae, Jae-Ho,Kim, Sun-Hee,Song, Jiale,Ha, Chang-Sik The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.47

<P>To prepare a versatile solid-state fluorescent sensor for chemical and biological applications is still a challenge. In this report, we describe a multifunctional fluorescent nanosensor that is capable of highly sensitive and selective detecting of the heavy metal ion Hg<SUP>2+</SUP> in a range of pH from 4.0 to 6.6, sensitive probing of α-amino acids by a metal ion-mediated approach, turn-on sensing of the protein BSA under physiological conditions and <I>in vitro</I> monitoring of Hg<SUP>2+</SUP> within living cells. This all-round smart sensor <B>1</B> has been designed by functionalizing highly ordered mesoporous SBA-15 nanoparticles with 3-isocyanatopropyltriethoxysilane and dansylcadaverine, followed by the characterization of the pore structure, textural property, microscopic morphology, molecular composition of grafted organic fluorophore and the colorimetric property of <B>1</B>. The quenching fluorescence emission of <B>1</B> in the presence of Hg<SUP>2+</SUP> could be attributed to the photoinduced electron transfer (PET) from the exited dansyl moiety to the proximate mercuric ion. The successful sensing of α-amino acids has been realized by an indirect approach, in which the quenched fluorescence of <B>1</B> by Hg<SUP>2+</SUP> was recovered upon the addition of α-amino acids. The turn-on sensing of BSA under physiological conditions is based on the hydrophobic interaction between BSA and the naphthalene ring on the dansyl fluorophore of <B>1</B>. This interaction also makes <B>1</B> become an efficient adsorbent for storing and retaining BSA. Finally, <B>1</B> has been demonstrated to have application in living cells by detecting intracellular Hg<SUP>2+</SUP>.</P> <P>Graphic Abstract</P><P>A versatile solid-state fluorescent sensor has been designed and prepared for chemical and biological applications. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm33103a'> </P>

Novel fluorinated polysilsesquioxane hollow spheres: synthesis and application in drug release

Dong, Fuping,Guo, Wanping,Chu, Sang-Wook,Ha, Chang-Sik Royal Society of Chemistry 2010 Chemical communications Vol.46 No.40

<P>Fluorinated polysilsesquioxane (FPSQ) hollow spheres with a large empty interior were synthesized in an aqueous medium by using (trifluoropropyl)trimethoxysilane as the sole precursor. The drug release applications of these spheres were demonstrated, and the materials have great potential as fluorinated drug release carriers.</P> <P>Graphic Abstract</P><P>Novel fluorinated polysilsesquioxane hollow spheres with a large empty interior were synthesized <I>via</I> hydrolytic polycondensation in aqueous medium for drug delivery application. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0cc01658f'> </P>

Uniform and monodisperse polysilsesquioxane hollow spheres: synthesis from aqueous solution and use in pollutant removal

Dong, Fuping,Guo, Wanping,Park, Sung-Soo,Ha, Chang-Sik Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.29

<P>A series of organofunctional polysilsesquioxane hollow spheres were prepared by an environment-friendly method. The use of this simple and reproducible synthetic approach resulted in highly uniform and monodisperse hollow spheres with high coverage of functional groups, namely, fluoroalkyl, phenyl, vinyl and methyl groups. By adjusting the precursor concentration, the particle size and shell thickness could be precisely controlled. The structure and functionality of these hollow spheres were characterized by field-emission scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, solid-state NMR spectroscopy, X-ray diffraction and nitrogen adsorption–desorption studies. These materials were used as adsorbents to treat the benzophenone-containing wastewater, and it was found that the hydrophobic contaminants could be effectively removed. The hydrophobic surface property of the polysilsesquioxane hollow spheres was found to be an important factor that influences the adsorption capacity.</P> <P>Graphic Abstract</P><P>A series of uniform and monodisperse polysilsesquioxane hollow spheres were synthesized in aqueous medium for pollutant removal. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm11337b'> </P>

A Monascus pilosus MS-1 strain with high-yield monacolin K but no citrinin

Feng, Yanli,Chen, Wanping,Chen, Fusheng 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

Monacolin K (MK) produced by Monascus spp. is mostly used to regulate cholesterol level, while it can be contaminated by citrinin, a mycotoxin yielded by some Monascus strains. To develop production of citrinin-free MK, an M. sp. MS-1 strain, identified as M. pilosus by morphologies and internal transcribed spacer sequences analysis, was isolated from red fermented rice. After 14 days of fermentation, yields of citrinin-free MK could be up to 0.58 mg/mL and 16.45 mg/g in liquid- and solid-state fermentation products under optimal conditions, respectively. Further determination revealed that no citrinin biosynthesis related genes such as ctnA, pksCT, ctnE, and ctnR were detected. Thus, HPLC combined with citrinin-related gene analyses can be used for rapid screening of non-citrinin production Monascus strains.

Triblock Copolymer Synthesis of Periodic Mesoporous Organosilicas

( Chang-sik Ha ),( Wanping Guo ),( Ii Kim ) 한국공업화학회 2003 한국공업화학회 연구논문 초록집 Vol.2003 No.-

Cytochrome P450 monooxygenase analysis in free-living and symbiotic microalgae Coccomyxa sp. C-169 and Chlorella sp. NC64A

Ntsane Trevor Mthakathi,Ipeleng Kopano Rosinah Kgosiemang,Wanping Chen,Molikeng Eric Mohlatsane,Thebeyapelo Jacob Mojahi,Jae-Hyuk Yu,Samson Sitheni Mashele,Khajamohiddin Syed 한국조류학회I 2015 ALGAE Vol.30 No.3

Microalgae research is gaining momentum because of their potential biotechnological applications, including the generation of biofuels. Genome sequencing analysis of two model microalgal species, polar free-living Coccomyxa sp. C-169 and symbiotic Chlorella sp. NC64A, revealed insights into the factors responsible for their lifestyle and unravelled biotechnologically valuable proteins. However, genome sequence analysis under-explored cytochrome P450 monooxygenases (P450s), heme-thiolate proteins ubiquitously present in species belonging to different biological kingdoms. In this study we performed genome data-mining, annotation and comparative analysis of P450s in these two model algal species. Sixty-nine P450s were found in two algal species. Coccomyxa sp. showed 40 P450s and Chlorella sp. showed 29 P450s in their genome. Sixty-eight P450s (>100 amino acid in length) were grouped into 32 P450 families and 46 P450 subfamilies. Among the P450 families, 27 P450 families were novel and not found in other biological kingdoms. The new P450 families are CYP745-CYP747, CYP845-CYP863, and CYP904-CYP908. Five P450 families, CYP51, CYP97, CYP710, CYP745, and CYP746, were commonly found between two algal species and 16 and 11 P450 families were unique to Coccomyxa sp. and Chlorella sp. Synteny analysis and gene-structure analysis revealed P450 duplications in both species. Functional analysis based on homolog P450s suggested that CYP51 and CYP710 family members are involved in membrane ergosterol biosynthesis. CYP55 and CYP97 family members are involved in nitric oxide reduction and biosynthesis of carotenoids. This is the first report on comparative analysis of P450s in the microalgal species Coccomyxa sp. C-169 and Chlorella sp. NC64A.