Preliminary Proteomic Analysis of Indomethacin's Effect on Tumor Transplanted with Colorectal Cancer Cell in Nude Mice

Wang, Yu-Jie,Zhang, Gui-Ying,Xiao, Zhi-Qiang,Wang, Hong-Mei,Chen, Zhu-Chu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.2

Nonsteroidal anti-inflammatory drugs such as indomethacin (IN) can exert anti-colorectal cancer (CRC) activity through cyclooxygenase independent mechanism, but the exactly biological mechanism is not completely known. Here we use proteomic tools to investigate the molecular mechanism of this action. First, nude mice bearing tumors derived from subcutaneous injection with human CRC cell line HCT116 were randomly allocated to groups treated with or without indomethacin. Later, tumor lumps were incised and then total proteins extracted. After separated with two-dimensional electrophoresis, thirty-one differently expressed spots were found between IN-treated and non-IN-treated groups, of which 25 spots decreased and 6 spots increased in abundance in IN-treated group. Through matrix-assisted laser desorption ionization time of flight mass spectrometry and then NCBInr and SWISS-PROT databases searching, 12 protein spots were finally identified including galectin-1, annexin A1, annexin IV, trancription factor BTF3A, calreticulin. Most of the identified proteins are correlated with tumor's biological prosperities of proliferation, invasion, apoptosis and immunity, or take part in cell's signal transduction. From above we thought that indomethacin can exert its effect on colorectal cancer through regulating several proteins' expression directly or indirectly. Further study of these proteins may be helpful in founding new targets of drugs for cancer chemotherapy.

從《東医宝鑒》的編撰特点探討許浚的學術思想

Ying, Wang(王英) 한국의사학회 2010 한국의사학회지 Vol.23 No.2

Dong-Eui-Bo-Gam is a general medical literature,writing by Korea physician Xujun,who makes reference of Chinese medical literatures、Taoist literatures、historical records、Confucian literatures and so forth prior Ming Dynasty. It coveres many fields,such as medical theory、etiology、pulse theory、herb、prescription、internal medicine、surgery、gynecology、pediatrics、acupuncture、regimen、YunQi and so forth. Dong-Eui-Bo-Gam combines medical science and many others,using clustering arrangements,fully reflects Xujun’s academic thoughts,and his rich clinical experiences.

Updating Smartphone’s Exterior Orientation Parameters by Image-based Localization Method Using Geo-tagged Image Datasets and 3D Point Cloud as References

Wang, Ying Hsuann,홍승환,배준수,최윤조,손홍규 한국측량학회 2019 한국측량학회지 Vol.37 No.5

With the popularity of sensor-rich environments, smartphones have become one of the major platforms for obtaining and sharing information. Since it is difficult to utilize GNSS (Global Navigation Satellite System) inside the area with many buildings, the localization of smartphone in this case is considered as a challenging task. To resolve problem of localization using smartphone a four step image-based localization method and procedure is proposed. To improve the localization accuracy of smartphone datasets, MMS (Mobile Mapping System) and Google Street View were utilized. In our approach first, the searching for candidate matching image is performed by the query image of smartphone’s using GNSS observation. Second, the SURF (Speed-Up Robust Features) image matching between the smartphone image and reference dataset is done and the wrong matching points are eliminated. Third, the geometric transformation is performed using the matching points with 2D affine transformation. Finally, the smartphone location and attitude estimation are done by PnP (Perspective-n-Point) algorithm. The location of smartphone GNSS observation is improved from the original 10.204m to a mean error of 3.575m. The attitude estimation is lower than 25 degrees from the 92.4% of the adjsuted images with an average of 5.1973 degrees.

Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin

Ying Zhu,Hao-liang Zhang,Qi-ying Wang,Min-jing Chen,Lin-bo Liu 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.2

microRNA (miR)-612 shows anticancer activity in several types of cancers, yet its function in melanoma is still unclear. This study was undertaken to investigate the expression of miR-612 and its biological relevance in melanoma cell growth, invasion, and tumorigenesis. The expression and prognostic significance of miR-612 in melanoma were examined. The effects of miR-612 overexpression on cell proliferation, colony formation, tumorigenesis, and invasion were determined. Rescue experiments were conducted to identify the functional target gene(s) of miR-612. miR-612 was significantly downregulated in melanoma tissues compared to adjacent normal tissues. Low miR-612 expression was significantly associated with melanoma thickness, lymph node metastasis, and shorter overall, and disease-free survival of patients. Overexpression of miR-612 significantly decreased cell proliferation, colony formation, and invasion of SK-MEL-28 and A375 melanoma cells. In vivo tumorigenic studies confirmed that miR-612 overexpression retarded the growth of A375 xenograft tumors, which was coupled with a decline in the percentage of Ki-67-positive proliferating cells. Mechanistically, miR-612 targeted Espin in melanoma cells. Overexpression of Espin counteracted the suppressive effects of miR-612 on melanoma cell proliferation, invasion, and tumorigenesis. A significant inverse correlation (r = -0.376, P = 0.018) was observed between miR-612 and Espin protein expression in melanoma tissues. In addition, overexpression of miR-612 and knockdown of Espin significantly increased the sensitivity of melanoma cells to doxorubicin. Collectively, miR-612 suppresses the aggressive phenotype of melanoma cells through downregulation of Espin. Delivery of miR-612 may represent a novel therapeutic strategy against melanoma.

Investigating the Value of Information in Mobile Commerce: A Text Mining Approach

Wang, Ying,Aguirre-Urreta, Miguel,Song, Jaeki The Korea Society of Management Information System 2016 Asia Pacific Journal of Information Systems Vol.26 No.4

The proliferation of mobile applications and the unique characteristics of the mobile environment have attracted significant research interest in understanding customers' purchasing behaviors in mobile commerce. In this study, we extend customer value theory by combining the predictors of product performance with customer value framework to investigate how in-store information creates value for customers and influences mobile application downloads. Using a data set collected from the Google Application Store, we find that customers value both text and non-text information when they make downloading decisions. We apply latent semantic analysis techniques to analyze customer reviews and product descriptions in the mobile application store and determine the embedded valuable information. Results show that, for mobile applications, price, number of raters, and helpful information in customer reviews and product descriptions significantly affect the number of downloads. Conversely, average rating does not work in the mobile environment. This study contributes to the literature by revealing the role of in-store information in mobile application downloads and by providing application developers with useful guidance about increasing application downloads by improving in-store information management.

Seoul Road Network Traffic State Estimation from Floating Car Data

Ying Hsuan Wang,Suhong Yoo,Jisang Lee,Hong-Gyoo Sohn 대한공간정보학회 2018 한국공간정보학회 학술대회 Vol.2018 No.11

The International Influence of China’s Equipment Manufacturing Industry: Evidence from the WIOT

Wang Ying,Li Lan 한국유통과학회 2016 유통과학연구 Vol.14 No.10

Purpose - This study analyzes economic relations and mutual influence in the global equipment manufacturing industry (EMI) and China’s influence. Research design, data, and methodology - Data were collected from the World Input-Output Database (WIOT), looking at 16 countries. The sample time period was 2002-2011. Influence and motivation coefficients were calculated. Results - 1) China’s EMI showed a very strong influence coefficient, even surpassing world industrial powers like Japan, the USA, Germany, and Korea. 2) As for influence on added-value, China’s EMI motivation coefficient was ranked third in 2011, which meant it had a negligible effect on added-value. 3) From 2002 to 2011, both the influence and motivation coefficients of China’s EMI rose. Conclusions – China’s EMI has strong influence and motivation coefficients. It has a significant impact on the world EMI, especially on the total output of the global EMI. Additionally, during 2002 to 2011, the ranking of China’s EMI motivation coefficient improved year over year, and its economic efficiency obviously improved. By 2011, China’s EMI’s international influence was second only to the US and Japan.

Deficiency of Follistatin-Like Protein 1 Accelerates the Growth of Breast Cancer Cells at Lung Metastatic Sites

Ying Zhang,Xiaozhou Xu,Ying Yang,Jie Ma,Lulu Wang,Xiangzhi Meng,Bing Chen,Ling Qin,Tao Lu,Yan Gao 한국유방암학회 2018 Journal of breast cancer Vol.21 No.3

Purpose: Follistatin-like protein 1 (FSTL1) is a secreted glycoprotein that has been shown to play a role in various types of cancer. However, the clinical significance and function of FSTL1 in breast cancer have not been reported. We investigated the role of FSTL1 in breast cancer in this study. Methods: Enzyme-linked immunosorbent assays, western blot analysis, and reverse transcription polymerase chain reaction were used to monitor the expression of FSTL1 in breast cancer tissue and in serum samples from breast cancer patients. We employed a 4T1 breast cancer model and Fstl1+/− mice for in vivo studies. Hematoxylin and eosin staining, western blot analysis, and RNA sequencing were used to analyze the effect of FSTL1 on primary tumor growth and lung metastasis. Results: We demonstrated that the expression of FSTL1 is reduced in both the breast cancer tissue and the serum of breast cancer patients. We showed that reduced levels of FSTL1 in serum correlate with elevated expression of Ki-67 and epidermal growth factor receptor (EGFR) in cancer tissues. Moreover, lowered expression of FSTL1 was associated with decreased survival in breast cancer patients. Experiments on the Fstl1+/− mouse model established that FSTL1 deficiency had no effect on primary tumor growth, but increased the lung metastases of breast cancer cells, resulting in reduced survival of tumor-bearing mice. RNA sequencing found significantly reduced expression of Egln3 and increased expression of EGFR in Fstl1+/− mice. Thus, our results suggest that FSTL1 may affect the expression of EGFR through Egln3, inhibiting the proliferation of breast cancer cells at lung metastatic sites. Conclusion: In conclusion, we suggest a suppressor role of FSTL1 in breast cancer lung metastasis. Furthermore, FSTL1 may represent a potential prognostic biomarker and a candidate therapeutic target in breast cancer patients.

Restoration of dysregulated intestinal barrier and inflammatory regulation through synergistically ameliorating hypoxia and scavenging reactive oxygen species using ceria nanozymes in ulcerative colitis

Ying Zhang,Hengyu Lei,Pengchong Wang,Qinyuan Zhou,Jie Yu,Xue Leng,Ruirui Ma,Danyang Wang,Kai Dong,Jianfeng Xing,Yalin Dong 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Reactive oxygen species (ROS) overproduction and excessive hypoxia play pivotal roles in the initiation and progression of ulcerative colitis (UC). Synergistic ROS scavenging and generating O2 could be a promising strategy for UC treatment. Methods Ceria nanozymes (PEG-CNPs) are fabricated using a modified reverse micelle method. We investigate hypoxia attenuating and ROS scavenging of PEG-CNPs in intestinal epithelial cells and RAW 264.7 macrophages and their effects on pro-inflammatory macrophages activation. Subsequently, we investigate the biodistribution, pharmacokinetic properties and long-term toxicity of PEG-CNPs in mice. PEG-CNPs are administered intravenously to mice with 2,4,6-trinitrobenzenesulfonic acid-induced colitis to test their colonic tissue targeting and assess their anti-inflammatory activity and mucosal healing properties in UC. Results PEG-CNPs exhibit multi-enzymatic activity that can scavenge ROS and generate O2, promote intestinal epithelial cell healing and inhibit pro-inflammatory macrophage activation, and have good biocompatibility. After intravenous administration of PEG-CNPs to colitis mice, they can enrich at the site of colonic inflammation, and reduce hypoxia-induced factor-1α expression in intestinal epithelial cells by scavenging ROS to generate O2, thus further promoting disrupted intestinal mucosal barrier restoration. Meanwhile, PEG-CNPs can effectively scavenge ROS in impaired colon tissues and relieve colonic macrophage hypoxia to suppress the pro-inflammatory macrophages activation, thereby preventing UC occurrence and development. Conclusion This study has provided a paradigm to utilize metallic nanozymes, and suggests that further materials engineering investigations could yield a facile method based on the pathological characteristics of UC for clinically managing UC.

“Moonlight on the Left Hand” book redesign

Wang Ying 한국콘텐츠학회 2023 ICCC International Digital Design Invitation Exhib Vol.2023 No.12