Identification and expression patterns of 20-hydroxyecdysoneresponsive genes from Procambarus clarkii

Bao‑Jian Zhu,Lin Tang,Ying‑Ying Yu,Dao‑Jun Wang,Chao‑Liang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.6

Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.

A novel design method for improving collapse resistances of multi-story steel frames with unequal spans using steel braces

Zheng Tan,Wei-hui Zhong,Bao Meng,Shi-chao Duan,Hong-chen Wang3,Xing-You Yao,Yu-hui Zheng 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.2

The bearing capacities resisted by the two-bay beams of multi-story planar frames with unequal spans under column removal scenarios differ considerably owing to the asymmetric stress on the left and right beams connected to the failed column and cause the potential for beams with larger span-to-depth ratios to be unable to exert effectively, which is disadvantageous for resisting the vertical load in unequal-span frame structures. To address this problem, the structural measure of adding braces to the weak bays of multi-story unequal-span frames was proposed, with the objective of achieving a coordinated stress state in two-bay beams with unequal spans, thereby improving the collapse resistance of unequal-span frame structures. Before conducting the numerical simulation, the modeling methods were verified by previous experimental results of two multi-story planar frames with and without steel braces. Thereafter, the effects of the tensile and compressive braces on the collapse behavior of the frame structures were elucidated. Then, based on the mechanical action laws of the braces throughout the collapse process, a detailed design method for improving the collapse resistance of unequal-span frame structures was proposed. Finally, the proposed design method was verified by using sufficient example models, and the results demonstrated that the design method has good application prospects and high practical value.

Inhibition of Microcystis aeruginosa by the Extracellular Substances from an Aeromonas sp

( Yu Mei Liu ),( Ming Jun Chen ),( Meng Hui Wang1 ),( Rui Bao Jia ),( Li Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algaelysis effects of lysine and clavulanate were confirmed.

The detection efficiency study of NaI(Tl) scintillation detector with the different numbers of SiPMs

Wang Bao,Zhang Xiongjie,Wang Qingshan,Wang Dongyang,Li Dong,Xiahou Mingdong,Zhou Pengfei,Ye Hao,Hu Bin,Zhang Lijiao 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.7

SiPMs are generally coupled into whole columns in gamma energy spectrum measurement, but the relationship between the distribution of whole SiPM columns and the energy resolution of the measured energy spectra is rarely reported. In this work, ∅ 3 3 inch NaI scintillator is placed on an 8 8 SiPM array, and the energy resolution of the 137Cs peak at 662 keV corresponding to the g-ray is selected as a reference. Each SiPM is switched to explore the influence of the number of SiPM arrays, distribution position, and reflective layer on the energy resolution of SiPMs. Results show that without coupling, the energy resolution is greatly improved when the number of SiPMs ranges from 4 to 32. However, after 32 slices (the area covered by SiPMs relative to the scintillator reaches 25.9%), the improvement in energy resolution and total pulse count is not obvious. In addition, the position of SiPMs relative to the scintillator does not exert much impact on the energy resolution. Results also indicate that by adding a reflective film (ESR), the energy resolution of the tested group increases by 10.38% on average. This work can provide a reference for the design and application of miniaturized SiPM gamma spectrometers

Separation Technology, Thermodynamics : A new model for predicting solute solubility in supercritical fluids based on the Wilson equation

( Bao Hua Wang ),( Qun Sheng Li ),( Ze Ting Zhang ),( Jin Miao Yang ),( Yan Cheng Liu ) 한국화학공학회 2006 Korean Journal of Chemical Engineering Vol.23 No.1

Creep properties and damage model for salt rock under low-frequency cyclic loading

Wang, Jun-Bao,Liu, Xin-Rong,Liu, Xiao-Jun,Huang, Ming Techno-Press 2014 Geomechanics & engineering Vol.7 No.5

Triaxial compression creep tests were performed on salt rock samples using cyclic confining pressure with a static axial pressure. The test results show that, up to a certain time, changes in the confining pressure have little influence on creep properties of salt rock, and the axial creep curve is smooth. After this point, the axial creep curve clearly fluctuates with the confining pressure, and is approximately a straight line both when the confining pressure decreases and when it increases within one cycle period. The slope of these lines differs: it is greater when the confining pressure decreases than when it increases. In accordance with rheology model theory, axial creep equations were deduced for Maxwell and Kelvin models under cyclic loading. These were combined to establish an axial creep equation for the Burgers model. We supposed that damage evolution follows an exponential law during creep process and replaced the apparent stress in creep equation for the Burgers model with the effective stress, the axial creep damage equation for the Burgers model was obtained. The model suitability was verified using creep test results for salt rock. The fitting curves are in excellent agreement with the test curves, so the proposed model can well reflect the creep behavior of salt rock under low-frequency cyclic loading. In particular, it reflects the fluctuations in creep deformation and creep rate as the confining pressure increasing and decreasing under different cycle periods.

Prognostic Role of Hepatoma-derived Growth Factor in Solid Tumors of Eastern Asia: a Systematic Review and Meta-Analysis

Bao, Ci-Hang,Liu, Kun,Wang, Xin-Tong,Ma, Wei,Wang, Jian-Bo,Wang, Cong,Jia, Yi-Bin,Wang, Na-Na,Tan, Bing-Xu,Song, Qing-Xu,Cheng, Yu-Feng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Hepatoma-derived growth factor (HDGF) is a novel jack-of-all-trades in cancer. Here we quantify the prognostic impact of this biomarker and assess how consistent is its expression in solid tumors. A comprehensive search strategy was used to search relevant literature updated on October 3, 2014 in PubMed, EMBASE and WEB of Science. Correlations between HDGF expression and clinicopathological features or cancer prognosis was analyzed. All pooled HRs or ORs were derived from random-effects models. Twenty-six studies, primarily in Eastern Asia, covering 2,803 patients were included in the analysis, all of them published during the past decade. We found that HDGF overexpression was significantly associated with overall survival (OS) ($HR_{OS}=2.35$, 95%CI=2.04-2.71, p<0.001) and disease free survival (DFS) ($HR_{DFS}=2.25$, 95%CI =1.81-2.79, p<0.001) in solid tumors, especially in non-small cell lung cancer, hepatocellular carcinoma and cholangiocarcinoma (CCA). Moreover, multivariate survival analysis showed that HDGF overexpression was an independent predictor of poor prognosis ($HR_{OS}=2.41$, 95%CI: 2.02-2.81, p<0.001; $HR_{DFS}=2.39$, 95%CI: 1.77-3.24, p<0.001). In addition, HDGF overexpression was significantly associated with tumor category (T3-4 versus T1-2, OR=2.12, 95%CI: 1.17-3.83, p=0.013) and lymph node status (N+ versus N-, OR=2.37, 95%CI: 1.31-4.29, p=0.03) in CCA. This study provides a comprehensive examination of the literature available on the association of HDGF overexpression with OS, DFS and some clinicopathological features in solid tumors. Meta-analysis results provide evidence that HDGF may be a new indicator of poor cancer prognosis. Considering the limitations of the eligible studies, other large-scale prospective trials must be conducted to clarify the prognostic value of HDGF in predicting cancer survival.

≪漢韓成語學習詞典≫編纂的基礎硏究

( Bao Xia Wang ) 한국중국언어학회 2014 중국언어연구 Vol.0 No.50

본고는 <중한성어학습사전>을 편찬하기 위한 기초 연구로서 사전 편찬의 방향과 기술 방식을 거시 구조와 미시 구조로 나누어 제안하는 데 목적이 있다. 2장에서는 중국어 성어의 개념과 사전학 특성을 서술하였다. <중한성어학습사전>은 제2외국어로서 중국어 성어를 학습하는 한국인을 위한 이중 언어 사전이다. 이는 사전 사용자가 중국어 성어의 의미를 이해하는 데에 도움을 주어야 할 뿐만 아니라 사용자가 중국어 성어를 정확하게 사용할 수 있도록 도와주는 역할도 해야 한다. 위와 같은 성격의 사전을 편찬하기 위해 3장에서는 사전의 거시 구조 중에 가장 중요한 문제인 중국어 성어 표제어 선정에 대해 살폈다. 중국어 성어 표제어 선정에서는 기존 사전이나 신문, 교재에 두루 등재된 성어를 데이터베이스화한 뒤, 학습용 성어, 고빈도 성어를 사전의 규모와 목적에 따라 그 수를 조정하는 안을 제시하였다. 그리고 규범적인 성어를 선택하여 주표제어로 등재하고 주표제어와 부표제어를 선택하는 데에 엄격하고 통일된 기준을 설정해야 한다고 주장하였다. 4장에서는 미시 항목 기술에 관해 다루었다. 중국어 성어의 병음 정보에서는 성조의 변화, 구어와 문어의 발음 정보를 제시한다고 제안하였다. 뜻풀이에서는 성어의 파생의미나 비유의미는 물론, 기본의미도 제시해야 하고, 성어의 구성요소인 형태소의 대역어, 성어의 어원도 제시해야 한다고 주장하였다. 그리고 중국어 성어와 한국어 대역 표현 간의 여러 유형을 나누고 그에 따른 뜻풀이 방식을 제시하였다. 문법 정보에서는 성어의 품사 성격, 구조, 문형 정보와 제약 정보 제시 방법을 예를 통해 밝혔다. 그밖에 용례 처리 원칙과 세부 지침, 관련어 정보 기술 방안도 구체적인 예를 통해 제시하였다.

ODV-Associated Proteins of the <i>Pieris rapae</i> Granulovirus

Wang, Xiao-Feng,Zhang, Bao-Qin,Xu, Hai-Jun,Cui, Ying-Jun,Xu, Yi-Peng,Zhang, Min-Juan,Han, Yeon Soo,Lee, Yong Seok,Bao, Yan-Yuan,Zhang, Chuan-Xi American Chemical Society 2011 JOURNAL OF PROTEOME RESEARCH Vol.10 No.6

<P><I>Alphabaculovirus</I> (lepidopteran-specific nucleopolyhedroviruses, NPV) and <I>Betabaculovirus</I> (granuloviruses, GV) are two main genera of the family Baculoviridae. The virion proteomes of <I>Alphabaculovirus</I> have been well studied; however, the <I>Betabaculovirus</I> virion compositions remain unclear. <I>Pieris rapae</I> granulovirus (PrGV) can kill larvae of <I>P. rapae</I>, a worldwide and important pest of mustard family crops. In this study, the occlusion-derived virus (ODV)-associated proteins of PrGV were identified using three mass spectrometry (MS) approaches. The MS analyses demonstrated that 47 proteins were present in PrGV-ODV. Of the 47 PrGV-ODV proteins, 33 have homologues identified previously in other baculovirus ODV/BVs, whereas 14 (P10, Pr21, Pr29, Pr35, Pr42, Pr54, P45/48, Pr83, Pr84, Pr89, Pr92, Pr111, Pr114 and FGF3) were newly identified ODV proteins. Seven of the 14 newly identified ODV proteins are specific to <I>Betabaculovirus</I>, including Pr35, Pr42, Pr54, Pr83, Pr84, Pr111 and Pr114. Furthermore, the data derived from these MS approaches were validated by immunoblotting analysis using antisera prepared from 11 randomly selected recombinant PrGV-ODV proteins (including 5 <I>Betabaculovirus</I>-unique proteins). Comparison analyses revealed the similar and different compositions between <I>Betabaculovirus</I> and <I>Alphabaculovirus</I> virions, which deepen our understanding of the baculovirus virion structure and provide helpful information on <I>Betabaculovirus</I>–host interaction studies.</P><P>We used three mass spectrometry (MS) approaches to identify the occlusion-derived virus (ODV)-associated proteins of the <I>Pieris rapae</I> Granulovirus. A total of 47 proteins were identified; 14 of them were first identified in the ODV, and 7 are specific to Granulovirus.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2011/jprobs.2011.10.issue-6/pr2000804/production/images/medium/pr-2011-000804_0002.gif'></P>

An improved Maxwell creep model for salt rock

Wang, Jun-Bao,Liu, Xin-Rong,Song, Zhan-Ping,Shao, Zhu-Shan Techno-Press 2015 Geomechanics & engineering Vol.9 No.4

The creep property of salt rock significantly influences the long-term stability of the salt rock underground storage. Triaxial creep tests were performed to investigate the creep behavior of salt rock. The test results indicate that the creep of salt rock has a nonlinear characteristic, which is related to stress level and creep time. The higher the stress level, the longer the creep time, the more obvious the nonlinear characteristic will be. The elastic modulus of salt rock decreases with the prolonged creep time, which shows that the creep damage is produced for the gradual expansion of internal cracks, defects, etc., causing degradation of mechanical properties; meanwhile, the creep rate of salt rock also decreases with the prolonged creep time in the primary creep stage, which indicates that the mechanical properties of salt rock are hardened and strengthened. That is to say, damage and hardening exist simultaneously during the creep of salt rock. Both the damage effect and the hardening effect are considered, an improved Maxwell creep model is proposed by connecting an elastic body softened over time with a viscosity body hardened over time in series, and the creep equation of which is deduced. Creep test data of salt rock are used to evaluate the reasonability and applicability of the improved Maxwell model. The fitting curves are in excellent agreement with the creep test data, and compared with the classical Burgers model, the improved Maxwell model is able to precisely predict the long-term creep deformation of salt rock, illustrating our model can perfectly describe the creep property of salt rock.