Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

철근 콘크리트 전단벽과 강재 보 전단접합부의 내진거동

이원호,윤현도,강대언,송한범,태경훈,박완신 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Ductile coupled flexural wall is the primary seismic load resisting system of buildings. In these systems, connections are generally headed stud bolt connection. The purpose of experimental study is to evaluate the seismic behavior of these connection under cyclic loading. A comprehensive experimental test involving 4 specimens has been performed and this program is used to study the effect of value of moment and edge distance. Through experimental test, the seismic behavior of typical headed stud bolt connections was established.

Java/qmail을 활용한 인터넷 메시지 시스템 설계 및 구현

이호성,이충세,조완섭 충북대학교 컴퓨터정보통신 연구소 2001 컴퓨터정보통신연구 Vol.9 No.1

90년대 중반부터 국내에 인터넷 붐이 일어나고 사용이 보편화되면서 전자메일은 개인간의 메시지를 가능하게 해주는 기반기술 중 하나로 자리잡고 있다. 기존의 POP(Post Office Protocol) 프로토콜을 이용하던 전자메일 시스템은 별도의 환경설정 없이 지역, 언어, 시간에 제약이 없는 웹기반의 메시지 시스템으로 발전하고 있다. 현재 웹기반 메시지 시스템에 사용되어지는 MTA(Mail Transfer Agent)로는 sendmail이 사실상의 표준으로 받아 들여지고 있다. 그러나 sendmail은 단일 모듈로 작성되었으며, 사이즈가 매우 크고, 보안상의 문제점도 지적되고 있다. 이에 본 논문에서는 sendmail의 문제점을 효율적으로 해결할 수 있는 qmail의 활용방법을 제시하고, 웹과 qmail을 연계할 수 있도록 자바를 통한 웹기반 인터넷 메시지 시스템을 설계 및 구현한다. The rapid growth of the internet and World-Wide Web(WWW) has allowed more people access to global computer network and network applications. Internet Service Provider profit by offering many kinds of Internet services such as electronic mail, WWW, FTP, and others. Electronic mail service is one of the most popular and essential services in Internet. so, it called "killer application". Mail server is a computer system that sends and receives electronic message for a number of users in a certain management domain. Mail servers are connected to networks and should reliably process incoming and outgoing mail messages. Sending and receiving tasks are actually processed by a special software called mail transfer agent in the mail server(e.g., sendmail or qmail). In this paper, we present the design and implementation of Internet message system. The system integrates two different technologies - the qmail an Java servlet. Our system combines advantages from both technologies so users may remotely manage their mail system easily and effectively.

건설업 근로자의 스트레스 실태에 관한 조사연구

이영섭,엄정현,김완진 서울産業大學校 2005 논문집 Vol.54 No.1

In the modern society, job stresses are the one of the causes of diseases related to the job, such as the cardiovascular diseases, the musculoskeletal diseases and so on. An investigation study of job stresses in the construction industry has not been conducted yet in Korea, even though job stresses are the causes of significant job-related diseases. An investigation study in the job stresses are carried out through questionaire and analyzed statistically with being Appeared by Stress Index. The Stress level is calculated by statistical analysis of each stressor through interrelationship between variables, The result of this study could be contributed to the control management of job stresses of construction industry with basic data for job stresses of work-related diseases.

退溪先生의 學統과 竹川先生의 位相

李完栽 慶北大學校 退溪硏究所 2003 退溪學과 韓國文化 Vol.- No.32

退溪先生의 학통에 대해서는 간단히 언급을 하면, 첫째 퇴계의 학문 연원은 宋代의 유교를 철학화하여 性理學을 집대성한 朱子이고, 둘째 학문 내용은 1. 종래 유학의 철학적 이해, 2. 윤리의 근거 확립, 3. 윤리 도덕의 주체 확립으로 요약하고, 셋째 그 流派에 있어서 鶴峰 金誠一, 西厓 柳成龍, 寒岡 鄭逑 계통의 학맥을 소개했다. 그리고 竹川 선생의 位相에 대해서는 先儒의 규정에 따라 1. 연원의 올바름, 2. 학문의 순수함과 독실함, 3. 節義의 뚜렷함을 검증했다. 그리고 竹川先生 그러한 학문과 인격이 퇴계 선생의 교화에 크게 힘입었다는 점을 밝혔다. 요컨대, 퇴계 선생의 학문은 성리학적 학문 원리에 입각하여 유학적 인생과 즉 도덕적 인간의 확립을 도모하려 하였고, 竹川 선생의 삶이 또한 퇴계 선생이 추구했던 그 삶을 충실하게 실천한 것이었다. 여기서 두 선생의 사상과 삶은 首尾가 一貫하는 것이라고 할 수 있다. 특히 竹川 선생의 독실한 踐履는 퇴계 선생의 삶의 모습에서 더욱 철저하게 배운 것이라고 할 것이다. 끝으로 퇴계 선생의 학맥을 일반적으로 앞에 든 3학파로 분류하나, 湖南地方의 제자로서 竹川 朴光前 선생, 高峰 奇大升 선생, 山天齊 李咸亨 선생 등이 계셨으니 필연코 湖南지역에도 퇴계 선생의 한 학맥이 면면히 흐르고 있었으리라 짐작된다. This thesis clarifies academical relations between ToeGye and his pupil JookChun, Park-Guang Zhen. The summary of content is as following. I classified Toe Gye's scholastic mantle into 3 parts. That is--- first, academic origin, second, academic details, third, academic school. 1. ToeGye's academic origin, he declared, it came from JuJa. 2. His academic details can be summarized into 3 points. First, he accepted the theory of JuJa and made traditional confucianism philosophized. Second, he emphasized the 'li'(理) and made firm ground of ethics. Third, he asserted 'Gyung'(敬) and made it clear that he established an ethical subject. 3. In the various school of ToeGye's study, there are mainly three schools;(HakBong Kim-Sung-il's) school, (SueAe Liu Sung Ryong's) school, (HanGang Hung-gu's) school. but I commented (Go-Bong Gi Dae Sueng, JookChun Park-Guang Zheun)-oriented (Ho-Nam) school can also be called one of ToeGye's study. About (JookChun Park-Guang Zheun)'s status, 1. He learned under ToeGye's instruction, his study can be called to be originated from ToeGye's. 2. By analyzing his written materials, his study is sincere and faithful to ToeGye's. 3. From the fact that he raised an army in the cause of justice loyalty when Japanese troops invaded Korea over the two times, we can notice that he definately adhered to his principle. In the conclusion, I think JookChun's status as commented above, came from the result that he tried to follow ToeGye's example and learn his scholarship and personality.

계단 오르기와 내리기 동안 다축범위(multi-radius) 무릎인공관절 수술자의 운동역학적 비교분석

진영완,유병인,곽이섭 한국운동역학회 2006 한국운동역학회지 Vol.16 No.1

복합 병렬 전단벽 접합부의 전단강도

박완신,한병찬,황선경,윤현도,이재연,이원호 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

An theoretical study of the strength of hybrid coupled shear wall connections is achieved. The bearing stress at failure in the concrete below the steel coupling beam section is related to the concrete compressive strength and the ratio of the width of the steel coupling beam section to the width of the hybrid coupled shear wall. Also experimental studies are achieved to revise shear transfer strength across connections between coupled shear walls and steel coupling beam. The main test variables were auxiliary details of stud bolts. In this studies. these proposed equations are shown to be in good agreement with the test results reported in the paper and with other test data in the literature.

日本의 海岸砂防에 관한 연구(II) : 沖繩縣의 海岸保安林을 중심으로;On Seaside Protection Forest in Okinawa Prefecture

全槿雨,李在善,朴完根,幸喜善福,中島勇喜,江崎次夫 江原大學校 森林科學硏究所 2004 Journal of Forest Science Vol.20 No.-

우리나라에 있어서 海岸林의 多面的 機能과 時代的 價値觀의 변화를 파악하고, 海岸砂防의 참고자료를 얻기 위해 일본의 海岸林과 海岸砂防에 대한 각종 자료를 수집·분석하고 있다. 전보(全槿雨 등, 2002)에 이어 日本 沖繩縣의 海岸防災林을 중심으로 海岸林의 槪要, 海岸保安林의 實態와 각 島嶼別 주요 海岸砂防用 樹種 및 대표적인 海岸保安林에 대하여 분석·정리하였다. Management system of coastal erosion-control forest in Japan together with coast sand dune fixation and stabilization were investigated and analyzed to introduce to Korean researchers the many-sided importance and function of coastal forest and its chage from the standpoint of social value. In this study some suggestions and ideas were proposed for establishment of coastal forest including some suggestions and idea were proposed for establishment of coastal forest including seaside protection forest characteristics and tree species for coastal dune fixation, based on the analysis of date which were collected from the seaside protection forest in Okinawa Prefecture in Japan.

TERUDERMIS를 이용한 골 혹은 건이 노출된 창상의 치료

최민호,이상복,황정욱,양완석,이강길 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Deep skin and soft tissue defects with exposed bone and tendon is difficult to treat, because skin graft rarely survives and flap surgery is sacrifice of donor site. Since "Stage I" membrane was developed by Yannas and Bruke in 1980, numerous kinds of artificial skin have been developed. The adaptability of "Terudermis", developed by the Terumo Co., as an artificial skin composed of sponge made of a fibrillar atelocollagen and a heat-denatured atelocollagen, was clinically evaluated on application to 13 cases presenting deep skin and soft tissue defect with exposed bones and tendons from October 1997 to march 1998. Terudermis has the advantage of allowing early incorporation of fibroblasts and capillaries into its collagen sponge due to very weak dehydrothermal cross-linking. Before Terudermis graft, several days of wet dressing and debridement were required to prepare healthy well- vascularized bed because Terudermis was weak on unsanitary wounds. After bed preparation, Terudermis was grafted like usual skin graft. Tie-over bolster dressing or compressive dressing was used case by case. The dressing was opened 2~3 days after Terudermis grafting. Wet dressing was done daily until the skin graft was done. Autologous skin graft was done 2∼3 weeks after Terudermis graft. Our clinical results indicated that Terudermis was beneficial in treating 77% of our patients. Through the use of this new method, treatment of severe skin and soft tissue defects that are usually treated by musculocutaneous or other conventional skin flaps can be replaced by Terudermis as an new artificial dermis.

개선된 중성염 전해공정을 이용한 모의 방사성금속폐기물의 제염

이지훈,육완이,양호연,하종현 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.2

원자력발전소에서 주로 발생되는 금속폐기물인 탄소강을 중성염전해질인 1.7M의 황산나트륨(Na₂SO₄)과 질산나트륨(NaNO₃)을 이용하여 기존전해제염과 개선된 전해제염공정의 비교실험을 수행하였다. 양극은 인코넬, 음극은 티타늄으로 하여 상온에서 1시간동안 반응시켜 금속폐기물 모재의 weight loss, 두께변화, 전해질 내 침전물농도, SEM을 이용하여 제염전후의 금속폐기물 표면의 형상을 분석하였다. 실험결과 개선된 전해제염 적용시 전해질 종류별 전류밀도 변화에 따른 실험에서는 전류밀도가 0.1∼0.6A/cm²으로 증가함에 따라 1.7M의 황산나트륨 적용시 금속폐기물 모재의 두께변화는 0.48±0.005∼67.7±0.02um, 1.7M의 질산나트륨 적용시에는 0.06±0.005∼17.7±0.05로 나타나 같은 전류밀도에서 황산나트륨 적용시 금속폐기물 모재의 표면 제염효율이 더욱 높은 양상을 보였다. 또한 전류밀도 0.3 A/cm² 및 1.7M의 황산나트륨의 조건에서 개선된 전해제염 적용 시 9.8±0.01um의 금속폐기물 두께변화를 보여 기존전해제염 적용시인 3.7±0.03um의 금속폐기물 두께변화보다 2배 이상의 표면 제염효과를 보였다. Conventional and modified electrolytic decontamination experiment were performed in the 1.7 M solution of sodium sulfate and sodium nitrate for decontamination of carbon steel as the simulated metal wastes which have been produced in large amounts from nuclear power plants. Anode and cathode were used as inconel and titanium respectively. The reaction time and temperature were 1 hr and 25℃. The analyses were performed of the characteristics such as weight loss and thickness change of metal waste, suspended solid in electrolyte and SEM observation. In modified electrolyte decontamination system with increased current density ranged from 0.1 to 0.6 A/cm², the metal waste showed thickness changes of 0.48±0.005 to 67.7±0.02um in 1.7 M sodium sulfate and those of 0.06±0.005 to 17.7±0.05um in sodium nitrate. Metal waste in modified electrolyte decontamination system showed the thickness change of 9.8±0.01um while it reacted up to 3.7±0.03um in conventional system with 0.3 A/cm² of current density and 1.7 M sodium sulfate. Decontamination efficiencies of modified electrolytic process are much higher than that of conventional electrolytic process when both are applied to metal waste.