http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Antioxidant and Antiaging Assays of Hibiscus sabdariffa Extract and Its Compounds
Wahyu Widowati,Andani Puspita Rani,R. Amir Hamzah,Seila Arumwardana,Ervi Afifah,Hanna Sari W. Kusuma,Dwi Davidson Rihibiha,Hayatun Nufus,Annisa Amalia 한국생약학회 2017 Natural Product Sciences Vol.23 No.3
Skin aging is a complex biological process due to intrinsic and extrinsic factors. Free radical oxidative is one of extrinsic factors that induce activation of collagenase, elastase and hyaluronidase. Natural product from plants has been used as antioxidant and antiaging. This study aimed to evaluate antioxidant and antiaging properties of Hibiscus sabdariffa extract (HSE) and its compounds including myricetin, ascorbic acid, and b carotene. The phytochemical of H. sabdariffa was determined using modified Farnsworth method and presence of phenols, flavonoids and tannins were in moderate content, whereas triterpenoids and alkaloids were in low content. Total phenolic content performed using Folin-Ciocalteu method, was 23.85 ?g GAE/mg. Quantitative analysis of myricetin, b-carotene, and ascorbic acid of HSE was performed with Ultra-High Performance Liquid Chromatography (UHPLC) that shows 78.23 ?g/mg myricetin, 0.034 ?g/mg b-carotene, whilst ascorbic acid was not detected. HSE has lower activity on DPPH (IC50 = 195.73 ?g/mL) compared to b-carotene, the lowest in ABTS assay (IC50 = 74.58 ?g/mL) and low activity in FRAP assay (46.24 ?M Fe(II)/?g) compared to myricetin, b-carotene. Antiaging was measured through inhibitory activity of collagenase, elastase, and hyaluronidase. HSE had weakest collagenase inhibitory activity (IC50 = 750.33 ?g/mL), elastase inhibitory activity (103.83 ?g/mL), hyaluronidase inhibitory activity (IC50 = 619.43 ?g/mL) compared to myricetin, b-carotene, and ascorbic acid. HSE contain higher myricetin compared to b-carotene. HSE has moderate antioxidants and lowest antiaging activities. Myricetin is the most active both antioxidant and antiaging activities.
Adaptive Gaussian Low-pass Pre-filtering for Perceptual Video Coding
Wahyu Wiratama,Yong-Jo Ahn,Ismail Marzuki,Donggyu Sim 대한전자공학회 2018 IEIE Transactions on Smart Processing & Computing Vol.7 No.5
This paper proposes adaptive Gaussian low-pass pre-filtering for perceptually highquality video encoding, considering the characteristics of human visual perception. The proposed pre-filtering adaptively removes high-frequency components that are not visually perceptible. In the proposed algorithm, a pixel-wise adaptive Gaussian filter is generated based on a local contrast threshold for a block that is not highly textured. Each pixel for the block is adaptively filtered, and the original pixel is replaced by the filtered value or a weighted one that is a mixture of the filtered and original values for edge preservation. The proposed algorithm can remove visually imperceptible components, and leads to bit reduction without visual deterioration of the coded videos. Experimental results show that the proposed algorithm can reduce the bitrate by up to 30% with minimal computational complexity and minimal visual quality loss for several video sequences.
< 전시-P-18 > Viscoelastic properties of bamboo
( Wahyu Dwianto ),( Danang Sudarwoko Adi ),( Teguh Darmawan ),( Mohamad Gopar ),( Lisman Suryanegara ) 한국목재공학회 2018 한국목재공학회 학술발표논문집 Vol.2018 No.1
This study relates to measurement of the viscoelastic properties of bamboo to answer why bamboo is more easily bent than wood, whether this is related to its anatomical structure or its chemical components. To answer this question, static bending testing on the bamboo was done in dry, fresh, and after softening conditions. Andong (Gigantochloa pseudoarundinaceae (Steud.) Widjaja) bamboo which has potential as raw material of bamboo lamination was used in this experiments. For static bending tests, the bamboo internode was cut 30cm in length, 2cm in width and with varying thickness depending on the location of the internode on the stem (20 internodes/stem). Measurement results indicating that the diameter of the internode and its wall thickness decreases from the bottom to the top of internode. This causes moisture content of fresh bamboo decreases from the bottom to the top of internode. Modulus of rupture (MOR) increases, but its modulus of elasticity (MOE) decreases. This is due to the increased density towards the top of internode. Increased density is associated with anatomical structure, where in the top of internode, the portion of vascular bundles are greater than the parenchyma cells. Elastic limits, in-elastic limits, and maximum deflections before fracture is gradually decreases with different bamboo conditions from dry, fresh, and after softening. Anatomical structures will further observed from various curved radius after bent as well as the contents of chemical components analyzed to know the effect on softening condition of bamboo. Furthermore, the viscoelastic properties of bamboo will be compared with wood with the same density. However, it can be predicted that bamboo can be more easily bent because there are many parenchyma cells with thin-walled cells in the inner parts and more easily softened.
Wahyu Widowati,Lusiana Darsono,Jo Suherman,Nurul Fauziah,Maesaroh Maesaroh,Pande Putu Erawijantari 한국생약학회 2016 Natural Product Sciences Vol.22 No.3
Inflammation plays an important role in host defense against external stimuli such as infection by pathogen, endotoxin or chemical exposure by the production of the inflammatory mediators that produced by macrophage. Anti-inflammatory factor is important to treat the dangers of chronic inflammation associated with chronic disease. This research aims to analyze the anti-inflammatory effects of Garcinia mangostana L. peel extract (GMPE), a-mangostin, and g-mangostin in LPS-induced murine macrophage cell line (RAW 264.7) by inhibiting the production of inflammatory mediators. The cytotoxic assay of G. mangostana L. extract, a-mangostin, and g-mangostin were performed by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to determine the safe and non-toxic concentration in RAW 264.7 for the further assay. The concentration of inflammatory mediators (COX-2, IL-6, and IL-1b) were measured by the ELISA-based assay and NO by the nitrate/nitrite colorimetric assay in treated LPS-induced RAW 264.7 cells. The inhibitory activity was determined by the reducing concentration of inflammatory mediators in treated LPS-induced RAW 264.7 over the untreated cells. This research revealed that GMPE, a-mangostin, and g-mangostin possess the anti-inflammatory effect by reducing COX-2, IL-6, IL-1b, and NO production in LPS-induces RAW 264.7 cells.
Isolation and characterization of AtXBP1, a functional homolog of XBP1/Hac1, in Arabidopsis
Wahyu Indra Fanata,Jeong Chan Moon,Joo Mi Jeon,Bo Hwa Son,In Jung Jung,Jae Yong Yoo,Jae Ho Cha,Je Hein Kim,Rikno Harmoko,Ki Seong Ko,Sang Yeol Lee,Kyun Oh Lee 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
A number of environmental and physiological stimuli, such as perturbation in calcium homeostasis, shift of endoplasmic reticulum (ER) redox potential, altered glycosylation and elevated protein synthesis lead to accumulation of unfolded or misfolded proteins in the ER lumen, and subsequently impose stress to ER. Such condition activates a set of signaling pathway termed the unfolded protein response (UPR). To date, a large number of components for UPR signaling pathway has been isolated and characterized in yeast or mammals. Plants also show a quite similar response compared to the UPR in yeast and mammals when they are exposure to the stimuli. However, the plant UPR and its signaling pathway are rarely studied. In this report, we present our research regarding to the characterization of a UPR signaling component AtXBP1 in Arabidopsis. AtXBP1 is alternatively spliced generating two isoforms; long isoform (AtXBP1L) and short isoform (AtXBP1S). Overexpression of AtXBP1S has shown to up regulate the expression of ER stress responsive genes. Futhermore, expression of the luciferase gene, driven by BiP3 promoter, under the ER stress condition has been significantly decreased in the atxbp1 mutant. Electrophoretic mobility shift assay revealed that only AtXBP1S is able to interact with putative XBP1 binding cis-elements in AtBiP2 and AtBiP3 promoters. These results support our hypothesis that the AtXBP1 is an important regulatory component in the UPR signaling in Arabidopsis. [Supported by BK21 program]
N-Acetylglucosaminyltransferase I (GnTI) is Developmentally essential in Rice (Oryza sativa)
Wahyu Indra Fanata,Jeong Chan Moon,Joo Mi Jeon,Bo Hwa Son,In Jung Jung,Jae Yong Yoo,Jae Ho Cha,Je Hein Kim,Rikno Harmoko,Ki Seong Ko,Sang Yeol Lee,Kyun Oh Lee 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
N-acetylglucosaminyltransferase I (GnTI) is an enzyme that controls the formation of hybrid and complex type N-glycans. Plant produce complex N-glycans with β1,2-xylose and core α1,3-fucose residues linked to the conserved core oligosaccharide. Production of heterologous glycoprotein in plant for therapeutic purposes is limited by the presence of plant specific oligosaccharide residues that are considered to arouse immunogenic response in the recipient body. Therefore, efforts for developing humanized N-glycosylation system in plants are required for the large scale production of safe therapeutic glycoproteins. In this regard, we isolated a rice mutant lacking GnTI activity. Biochemical analyses showed that rice gnt1 mutant predominantly produced high mannose type glycans and β1,2-xylose and core α1,3-fucose were absent on the endogenous glycoproteins. However, the rice gnt1 mutant showed severe developmental defects such as retarded shoot and root development, failure in tiller formation, and finally resulted in early developmental lethality. Interestingly, callus induced from gnt1 seeds was maintainable and proliferation rate of gnt1 callus was similar with that of WT. However, gnt1 calli were relatively smaller than that of WT. [Supported by EB-NCRC & BK21 program]
Plant made pharmaceuticals (PMP) as a convenient, safe and economical alternative to the shortfall
Wahyu Indra Fanata,In Jung Jung,Bo Hwa Son,Jae Yong Yoo,Je Hein Kim,Rikno Harmoko,Ki Seong Ko,Sang Yeol Lee,Kyun Oh Lee 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1
Since the completion of the human genome project, development of new therapeutic and diagnostic proteins have been exponentially increased, but widespread use of these molecules has been hampered by production bottlenecks such as low yields, poor and inconsistent product quality and a shortage of production capacity. Especially, we are facing a growing demand for protein diagnostics and therapeutics, but lack the capacity to meet those demands using established facilities. Over the last decade, plants have emerged as a convenient, safe and economical alternative to mainstream expression systems which are based on the large-scale culture of microbes or animal cells, or transgenic animals. The production of plant-made pharmaceuticals and technical proteins is known as Molecular Farming. The objective is to harness the power of agriculture to cultivate and harvest plants or plant cells producing recombinant therapeutics, diagnostics, industrial enzymes and green chemicals. Molecular Farming has the potential to provide virtually unlimited quantities of recombinant antibodies, vaccines, blood substitutes, growth factors, cytokines and enzymes for use as diagnostic and therapeutic tools in health care, the life sciences and the chemical industry. The overall aim of our research is to develop new plant molecular farming systems which are economic, efficient, stable and safe. To accomplish the purpose, we are performing specific research objectives in regards to plant molecular farming.
Poster Session : Characterization of DJ-1 Like gene in arabidopsis
( Wahyu Indra Duwi Fanata ),( Min Hee Jung ),( Joon Yung Cha ),( Netty Ermawati ),( Ying Shi Liang ),( Mukhamad Su`udi ),( Sun Ping ),( Sun Shin Cha ),( Kon Ho Lee ),( Dae Young Son ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-