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Marcelle Michelotti Bettoni,Átila Francisco Mogor,Volnei Pauletti,Nieves Goicoechea 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.5
The combined application of humic substances and mycorrhizal inocula under increased CO2 in the air is apromising horticultural technique for improving the quality of greenhouse-grown onion seedlings. The objective of thisstudy was to evaluate if the development and qualitative characteristics of bulbs from the yellow onion Allium cepaL. Alfa São Francisco improved with the application of the abovementioned agronomic, biotic, and environmentalfactors. The lowest dry matter was achieved in bulbs of onions not inoculated with mycorrhizal fungi, non-amendedwith humic acids (HA), and grown at ambient CO2. Mycorrhizal inoculation significantly increased bulb biomass inplants cultivated at ambient CO2, and not fertilized with HA, which may be a consequence of the enhanced acidphosphatase activity in roots. The application of HA always increased bulb dry matter, which were more evident atelevated rather than ambient CO2 conditions and correlated with improved acid phosphatase activity in the plants atelevated CO2. Mycorrhizal inoculation, HA supplementation, and elevated CO2 in the air had an additive effect ofincreasing the content of soluble sugars, proteins, and phenolics in bulbs. While high levels of sugars and proteinsenhanced the energetic value of onions, the increase in phenolics improved their antioxidant properties. The tripleinteraction between mycorrhization, HA fertilization, and elevated CO2 also increased the ratio between soluble solidsand total titratable acidity, which may enhance the perception of sweetness and make onions more pleasant forconsumption.
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Trametes villosa Lignin Peroxidase (TvLiP): Genetic and Molecular Characterization
( Rita Terezinha De Oliveira Carneiro ),( Maiza Alves Lopes ),( Marilia Lordelo Cardoso Silva ),( Veronica Da Silva Santos ),( Volnei Brito De Souza ),( Aurizangela Oliveira De Sousa ),( Carlos Primin 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1
White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7<sup>th</sup> day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.
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