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Vivian Molina,Miriam Noa,Lourdes Arruzazabala,Daisy Carbajal,Rosa M? 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.4
D-003 is a mixture of very-high-molecular-weight aliphatic acids purified from sugar cane wax (Saccharumofficinarum), which inhibits platelet aggregation and lipid peroxidation. The objective of the present study was to evaluatethe effect of D-003 on cerebral ischemia induced by ischemia-reperfusion (I-R) in Mongolian gerbils. Two experimental se-ries were conducted. The first series investigated the effects of D-003 on cerebral edema, neurological symptoms, and mor-tality in Mongolian gerbils with cerebral ischemia induced by I-R, while the second series investigated the effects on histo-logical markers of cerebral injury, such as edema intensity (vacuolization) and cerebral necrosis. Animals were randomlydistributed in five experimental groups: a sham-operated group experiencing surgical handling except the clamping and orallytreated with Tween/water vehicle and four groups subjected to the I-R surgical procedure. One of these groups was treatedwith the same vehicle, and the other three groups received D-003 at 25, 100, and 200 mg/kg, respectively. All treatments wereadministered for 14 days. D-003 (200 mg/kg) significantly reduced the cerebral edema and clinical symptoms provoked by I-R compared with the positive control group, whereas lower doses (25 and 100 mg/kg) were not effective. Positive controlanimals showed an injury profile characterized by swelling (tissue vacuolization) and necrosis of neurons in all areas of thebrain studied (frontal cortex, hippocampus, and striatum). The results of the histological study were consistent with those ob-served by determining cerebral edema and symptoms observation. Thus, D-003 at 200 mg/kg significantly reduced histolog-ical markers of brain injury (swelling and necrosis) compared with the control group. It is concluded that D-003 administeredorally at 200 mg/kg for 14 days protected against cerebral damage caused by bilateral cerebral ischemia in Mongolian ger-bils.
USAGE OF COLORED LED (LIGHT-EMITTING DIODE) ON SIGHT TRAINING OF RASBORA (RASBORA DANICONIUS)
Vivian LEE 국제과학영재학회 2014 APEC Youth Scientist Journal Vol.6 No.2
Environmental movements to return captured wildlife to nature are accelerating globally. From dolphins to killer whales, many species of marine organisms receive training to adjust to life in the wild. Different techniques can be utilized in behavioral training process including exposure to light sources (light training). Using classical training to teach Rasbora (Rasbora daniconius) to associate green light and food, other colors were tested to observe the optimum wavelength for behavioral training. Colored LED lights of blue, red, and white with short, long, and mixed wavelengths respectively were tested after complete training with green light which lasted ten days. The group of Rasbora was successfully trained to show feeding behavior when conditioned stimulus of green light was presented even in the absence of food. When introduced light of red and white, Rasbora showed feeding behavior almost identical to that of green light exposure. However, blue light elicited no clear feeding behavior in the group. As Rasbora have preference over light with long and mixed wavelengths such as red and white light, it can be inferred that red and white light is more effective in behavioral training. Blue light of short wavelength was found to be ineffective for detection of stimulus.The results demonstrated that the potential programs for returning wildlife back to nature can achieve success based on the understanding visual perceptions of marine animals such as those of rasbora.